A contrast between tense and lax vowels is a main feature of many Tibeto-Burman (TB) languages spoken in Southwest China. This study investigated the articulation and acoustics of tense-lax contrast in two Northern Yi dialects: Suondi and Yinuo. Both dialects have five pairs of phonologically-defined tense and lax vowels. Previous studies have shown that tense and lax vowels in TB had distinct patterns in phonation types and f0, while the difference in other acoustic dimensions—vowel quality, duration, and intensity are rarely examined, and the difference in the lingual gestures is largely unknown. The current study examined the articulatory and acoustic characteristics of tense-lax contrast using ultrasound imaging and spectrogram analysis. Our preliminary results showed that articulatorily, tense vowels were produced with more retracted tongue root. Acoustically, tense vowels had higher F1 and lower F2, which was caused by the lowered and retracted tongue position. The intensity of tense vowels was lower than that of lax vowels for front vowels. No significant difference was found in duration. Based on the articulatory and acoustic data, as well as the vowel harmony phenomenon in Yi, we propose that the tense-lax distinction in Northern Yi can be better represented as [+RTR] phonologically.