Stress Levels Research Articles

The Impact of Different Interruptions on Perceived Stress: Developing a Multimodal Measurement for Early Detection

This study presents the experimental findings investigating the effect of different interruptions on stress levels and validates indicators for interruption detection based on machine learning. Data were collected from one hundred and four subjects across simulated office-like tasks (i.e., writing and problem-solving) by four interruption types (i.e., distraction, intrusion, break, and multitasking). Behavioral data (RT and error rate), physiological signals (HR and EDR), and subjective ratings were recorded. Features based on ANOVA were extracted and entered into the classification models as inputs to examine how well different variables could be used to evaluate interruptions. Different stress outcomes were found on objective and subjective measures by interruption type. Also, we found that a combination of physiological signals, behavioral data, and subjective ratings provides the best accuracy. Additionally, the results suggest that task performance is a more reliable indicator than subjective ratings for interruption measurement and classification. This study uses methods that could be adapted for office workers, and the results provide preliminary support for developing an early interruption detection system. The methods employed in this research may benefit office staff, and the results offer preliminary support for developing an early interruption detection system to enhance worker health and company productivity.

Impact of Psychosocial Factors on the Activity of Crohn’s Disease: A Cross-Sectional Analysis of Social Support, Stress, and Flare-Up Incidence

Background/Objectives: Crohn’s disease is a chronic and debilitating intestinal disorder that alternates between remission and active flare-ups, often leading to hospitalization. Social support is known to enhance adaptation to the disease and modulate stress perception in patients, while stress may exacerbate symptoms. The aim of this study was to examine the roles of perceived stress and social support in Crohn’s disease and their impact on the frequency of flare-ups. Methods: A cross-sectional observational study was conducted, assessing stress and social support in a cohort of 91 patients with Crohn’s disease during flare-up and remission phases. The Perceived Stress Scale (PSS-14) and a Social Support Questionnaire were utilized for evaluation. We examined the relationship between stress and social support in Crohn’s disease. The interaction between the variables studied was also observed, considering the stage of the disease. Finally, we carried out an analysis of the influence of these two variables on the development of flare-ups in Crohn’s disease. Results: The study revealed that patients experience higher stress levels during flare-ups and that these levels are amplified by a lack of social support. A significant relationship was identified between the levels of social support and the occurrence of flare-ups, indicating that better social support is associated with fewer flare-ups. Conclusions: Patients with Crohn’s disease in the flare-up phase are subject to considerable stress. A deficit in social support is linked to an increase in stress levels. The interaction between social support and stress plays a critical role in the development of flare-ups.

Academic Stress among College Students

Academic stress refers to the strain experienced by students, resulting in a state of distress that often manifests itself as various psychological and behavioral challenges. This research investigates into the pervasive issue of academic stress among college students, emphasizing its consequences and gender disparities. Employing a comprehensive mixed-methods approach, the study utilized a structured questionnaire to quantitatively assess academic stress level among selected students of a college using the Academic Stress Scale for College Students (ESSCS) that featured 16 items with six response choices. Findings indicated a notable gender difference in academic stress levels, highlighting the importance of addressing societal factors rather than inherent gender distinctions. Female students reported higher stress levels, emphasizing the need to address sources like pressure, anxiety, conflict, and frustration to improve their mental well-being. The study calls for collaborative efforts among educational institutions, parents, and educators to reduce workloads and implement innovative teaching methods, fostering divergent thinking and supporting diverse abilities. It serves as a call to action for institutions to prioritize students' mental health and create a more balanced and nurturing academic experience, advocating for a holistic approach to address the multifaceted challenges associated with academic stress.

A New Remote Sensing Index for Forest Dryness Monitoring Using Multi-Spectral Satellite Imagery

Canopy water content is a fundamental indicator for assessing the level of plant water stress. The correlation between changes in water content and the spectral reflectance of plant leaves at near-infrared (NIR) and short-wave infrared (SWIR) wavelengths forms the foundation for developing a new remote sensing index, the Infrared Canopy Dryness Index (ICDI), to monitor forest dryness that can be used to predict the consequences of water stress. This study introduces the index, that uses spectral reflectance analysis at near-infrared wavelengths, encapsulated by the Normalized Difference Infrared Index (NDII), in conjunction with specific canopy conditions as depicted by the widely recognized Normalized Difference Vegetation Index (NDVI). Development of the ICDI commenced with the construction of an NDII/NDVI feature space, inspired by a conceptual trapezoid model. This feature space was then parameterized, and the spatial region indicative of water stress conditions, referred to as the dry edge, was identified based on the analysis of 10,000 random observations. The ICDI was produced from the combination of the vertical distance (i.e., under consistent NDVI conditions) from an examined observation to the dry edge. Comparisons between data from drought-affected and non-drought-affected control plots in the Australian Northern Jarrah Forest affirmed that the ICDI effectively depicted forest dryness. Moreover, the index was able to detect incipient water stress several months before damage from an extended drought and heatwave. Using freely available satellite data, the index has potential for broad application in monitoring the onset of forest dryness. This will require validation of the ICDI in diverse forest systems to quantify the efficacy of the index.

P.027 KNOW BRAIN EMBRACE CARE: A study investigating young adult stroke patients’ knowledge and behaviour around lifestyle

Background: Stroke incidence is rising among younger adults (≤65yrs). Modifiable and behavioural risk factors are linked to stroke; however, limited understanding of knowledge and behaviour exists around preventative/lifestyle medicine (LSM) among this patient population. Study aim was to assess younger adult stroke patients’ lifestyle knowledge, habits, and barriers. Methods: A cross-sectional design was employed. Data were collected through an online, self-reported survey following a routine stroke prevention clinic visit and analyzed using descriptive and inferential statistics. Results: Sample included 103 participants (56.3% women, 60% white, mean age 47.6, 54.5% prior stroke). Majority (63%) understood current healthy lifestyle recommendations around blood pressure, sleep, and alcohol use, but fewer (<24.3%) around exercise and diet. Almost 70% ate processed food weekly, with emotions and social/family situations influencing eating habits. Interestingly, despite not understanding the current recommendations, >80% exercised moderately (3.5d/wk) with work and family responsibilities as main barriers. Over 50% slept <7hrs/night, had moderate to high stress levels, and implemented different coping strategies (food, TV, video games, and exercise). Majority (82.4%) reported willingness to change habits. Conclusions: Our findings provide valuable insight on young adult stroke patients’ preventative/LSM-related knowledge, habits, and barriers and provide new opportunities for the development of brain care-related initiatives.

A role for curcumin in preventing liver fibrosis in animals: a systematic review and meta-analysis

ObjectiveThis meta-analysis aimed to determine the efficacy of curcumin in preventing liver fibrosis in animal models.MethodsA systematic search was conducted on studies published from establishment to November 2023 in PubMed, Web of Science, Embase, Cochrane Library, and other databases. The methodological quality was assessed using Sycle’s RoB tool. An analysis of sensitivity and subgroups were performed when high heterogeneity was observed. A funnel plot was used to assess publication bias.ResultsThis meta-analysis included 24 studies involving 440 animals with methodological quality scores ranging from 4 to 6. The results demonstrated that curcumin treatment significantly improved Aspartate aminotransferase (AST) [standard mean difference (SMD) = -3.90, 95% confidence interval (CI) (−4.96, −2.83), p < 0.01, I2 = 85.9%], Alanine aminotransferase (ALT)[SMD = − 4.40, 95% CI (−5.40, −3.40), p < 0.01, I2 = 81.2%]. Sensitivity analysis of AST and ALT confirmed the stability and reliability of the results obtained. However, the funnel plot exhibited asymmetry. Subgroup analysis based on species and animal models revealed statistically significant differences among subgroups. Furthermore, curcumin therapy improved fibrosis degree, oxidative stress level, inflammation level, and liver synthesis function in animal models of liver fibrosis.ConclusionCurcumin intervention not only mitigates liver fibrosis but also enhances liver function, while concurrently modulating inflammatory responses and antioxidant capacity in animal models. This result provided a strong basis for further large-scale animal studies as well as clinical trials in humans in the future.Systematic Review Registration:https://www.crd.york.ac.uk/prospero/, identifier CRD42024502671.

Prevalence of Stress Level in Physiotherapy Professors in Gujarat - An Observational Study

BACKGROUND: Physiotherapist as a professional, is impacting professionally and personally. Due to job instability and job viability especially for private practitioners which affecting psychological status. AIM: The aim of this study was to find out the prevalence of stress level in physiotherapy professors in Gujarat METHOD: The observational study was carried out among 103 physiotherapy professors. In which 69 female and 34 male who had willingness to participate in the study according to inclusion and exclusion criteria. Written consent was taken by individuals. The participants filled a Google self-designed, close-ended questionnaire of PSS (10 items) which was circulated via WhatsApp in different physiotherapy groups. Descriptive analysis was done using Microsoft excel. RESULT: 8.73% high stress,72.81% moderate stress,18.45% low stress found in physiotherapy professors. In this study 67.94% stress found in assistant professors,28.15% in associate professors and 3.88% in Principle. CONCLUSION: So, this study concluded that high prevalence of stress is found in Assistant Professors, moderate prevalence in Associate Professors and low prevalence in Principal. Study also depicting female perceiving high stress rather male. Keywords: Stress, Physiotherapy, Professor, Gujarat

Effects of Magnetized, Chelated Iron Foliage Treatments, and Metal Halide Lamps on Plant Water Structure, Water Vapor Dynamics, and Resilience for Legumes under Water Stress

A greenhouse study was conducted to determine the effects of foliar applications of magnetized, chelated liquid iron fertilizer for increasing the drought tolerance of two legumes. The study objectives were to determine the drought tolerance effects of four treatments on foliar gas exchange, soil moisture, and plant growth for soybean (Glycine max) and velvet bean (Mucuna pruriens) plants. The plant treatments included applications with chelated liquid iron fertilizer (2.5 and 5%) with a conventional boom sprayer, with and without magnets in the spray lines, and metal halide lamps. Three gas exchange measurements were collected before applying the foliage treatments and after two water stress treatments. A foliage and metal halide lamp treatment deactivated or unlinked nine interconnected gas exchange parameters that are correlated with plant defense activities during water stress conditions. The deactivation of interconnected regulatory gas exchange functions improved metabolic efficiency, reduced stress levels, and boosted plant resilience to abiotic stressors. Also, the study findings suggest that the study treatments maintained or increased the level of biologically structured water in plant tissues and vascular systems.

Comparison of stress, anxiety and depression levels of health, education and security sector employees: The effect of psychological resilience.

This study compared the stress, anxiety and depression levels in employees working in the health, education and security sectors. The study also investigated the effect of psychological resilience on stress, anxiety, and depression levels and whether the stress, anxiety, depression and psychological resilience levels of employees differ according to occupational and demographic variables. In this cross-sectional study, 1222 employees participated, comprising 50.8% from the health sector, 37.7% from education, and 11.5% from security. Data were collected using the Stress, Anxiety, and Depression Scale-21 and the Brief Resilience Scale. The study revealed varying rates of severe stress (49.1%), anxiety (74.0%), and depression (53.2%) among participants. Health employees experienced higher stress (52.1%), anxiety (77.0%), and depression (58%) rates compared to those in education (46.4%, 72.4%, and 48.4%) and security sectors (44.3%, 66.4%, and 48.5%). Health employees exhibited higher stress, anxiety, and depression levels, signifying a significant difference. Furthermore, the research identified psychological resilience as a crucial predictor of stress, anxiety, and depression. Additionally, these mental health issues were more prevalent among younger individuals with less work experience, females, private sector employees, singles, and those without children. The findings of the study showed that the level of stress, anxiety and depression was high in employees working in all three sectors (health, education and security), but mental problems were more common in health employees. The fact that psychological resilience is a significant predictor of stress, anxiety and depression levels indicates that this factor should be taken into account.

Understanding the demographics, training experiences and decision-making practices of UK triage nurses.

International research suggests that triage nurses' decision-making practices and training experiences vary significantly across emergency departments (EDs). However, there does not appear to be similar research published in the UK. Understanding factors, such as demographics, training and decision-making could provide a picture of triage nurses working in UK EDs, identify the interventions required to improve practice and inform further research. To explore the demographics, training experiences and decision-making practices of registered nurses who assess patient acuity at triage in UK EDs. The study used an online, descriptive, cross-sectional survey design. A total of 51 triage nurses from across the UK responded to the survey. Most (61) had achieved a bachelor's degree as their highest qualification, while 3 had postgraduate qualifications. Respondents had a median of seven years since qualifying, six years working in their current ED and five years working in triage and used a range of titles to describe their role. Low staffing and busy ED environments increased respondents' stress levels, which affected confidence in triage decision-making abilities among less experienced nurses. More experienced respondents coped with their stress by relying on their knowledge and skills. Not all respondents had received triage training, and for those that had, the training varied in type and frequency across EDs. Overall, respondents had low satisfaction with the amount, quality and content of the training they had received. There is a need for safer staffing levels in EDs and greater support for staff welfare. The development of national standards, incorporating defined knowledge and skills and set time periods for refresher training, is required to enhance triage practice in EDs.

A study on optimizing public spaces in rural heritage tourist sites from spatial perception: The case of Jinggang historic town

The development of cultural and rural tourism has fostered the expansion of heritage sites while concurrently presenting challenges such as resource wastage and cultural degradation. Optimizing spatial use requires a comprehensive understanding of the dynamics inherent to these sites. An emerging trend involves the integration of human perception technology into spatial perception research. Thus, to examine the correlation between alterations in physical and visual environments, tourists’ perceived changes, and primary influencing elements in rural heritage tourism sites, Jinggang – a historic and cultural town in Changsha, Hunan province, China – was selected as the research site. A comprehensive interdisciplinary analysis was conducted by integrating physical environment data from public spaces. These data include air temperature (Ta), relative humidity (RH), wind speed (WS), equivalent sound pressure level (ESPL), visual environment data (including green view index [GVI], sky view index [SVI], road and pavement view index (R&PVI), and construction view index [CVI]), along with biosensing data (level of stress [LS]) collected from tourists. The findings indicate the following: (i) the LS of the public space in Jinggang falls within the normal range but remains generally high, with the humanistic landscape, dominated by traditional architecture, scoring higher than the natural landscape; (ii) regarding spatial perception, the quantity of physical environment elements influencing LS surpasses that of visual environment elements, and the most relevant indicators, including ESPL, R&PVI, and CVI, all contribute to tourists’ LS; and (iii) ESPL significantly influences LS within the environment domain, where higher environmental noise decibels correspond to tourists’ increased LS. The study concludes that historic towns possess distinct characteristics as types of rural heritage tourism sites. This study provides valuable insights into enhancing spatial environmental quality and promoting an appealing and sustainable rural setting by implementing public space optimization strategies in rural heritage sites.

Factors influencing stress and resilience among Egyptian medical students: a multi-centric cross-sectional study

BackgroundPsychological stress is a common psychological comorbidity among medical students and worsens their quality of life. Psychological resilience is thought to have a protective role against stress. However, evidence regarding the prevalence of stress and resilience alongside their associated factors is scarce, especially in the Middle East. This is the first multicenter, cross-sectional study to investigate resilience and stress among Egyptian medical students.MethodsThe current cross-sectional study was conducted on 2465 university students in seven public universities in Egypt. The universities were selected using the simple randomization method. The data was collected using a self-administered questionnaire consisting of four parts: demographic data, socioeconomic tool represented in the Family Affluence Scale (FAS), the Kessler Psychological Distress Scale (K10), and the Brief Resilience Scale (BRS). Data was analyzed in SPSS version 26 software.ResultsThe majority of the students were stressed (86.5%), most of whom had severe stress (48.9%). Most of the students had low resilience (49.9%), while only 3.2% had high resilience. In the logistic regression analysis, being a female, living alone, spending long hours on social media, and thinking of suicide or leaving medicine were associated with being stressed and having low resilience. Medical students with low resilience were significantly more liable to stress [Adjusted odds ratio (AOR) = 3.667, confidence interval (CI): 2.709–4.965, P = 0.000], and vice versa [AOR = 3.709, CI: 2.746–5.009, P = 0.000]. Interestingly, high socioeconomic status showed a significant association with high resilience (P = 0.004); nonetheless, it was not associated with stress (P = 0.993). Academic grades were not associated with both the level of stress and resilience. Aging, being in clinical or academic stages, smoking, having a chronic disease, and being financially-supported are neither associated with stress nor resilience.ConclusionsThe present study revealed that Egyptian medical students had low resilience and high stress, with a significant relationship between both of them. Further investigations via longitudinal study design to understand the resilience-stress relationship are recommended. Developing and implementing resilience-improving strategies in medical schools is highly recommended to decrease the prevalence of stress and its subsequent burdens.

Differences in childhood stress between Neanderthals and early modern humans as reflected by dental enamel growth disruptions

Neanderthals’ lives were historically portrayed as highly stressful, shaped by constant pressures to survive in harsh ecological conditions, thus potentially contributing to their extinction. Recent work has challenged this interpretation, leaving the issue of stress among Paleolithic populations highly contested and warranting in-depth examination. Here, we analyze the frequency of dental enamel hypoplasia, a growth disruption indicator of early life stress, in the largest sample of Neanderthal and Upper Paleolithic dentitions investigated to date for these features. To track potential species-specific patterns in the ontogenetic distribution of childhood stress, we present the first comprehensive Bayesian modelling of the likelihood of occurrence of individual and matched enamel growth disruptions throughout ontogeny. Our findings support similar overall stress levels in both groups but reveal species-specific patterns in its ontogenetic distribution. While Neanderthal children faced increasing likelihoods of growth disruptions starting with the weaning process and culminating in intensity post-weaning, growth disruptions in Upper Paleolithic children were found to be limited around the period of weaning and substantially dropping after its expected completion. These results might, at least in part, reflect differences in childcare or other behavioral strategies between the two taxa, including those that were advantageous for modern humans’ long-term survival.

Stress testing for IInd pillar life-cycle pension funds using hidden Markov model

AbstractThis paper presents a stress testing technique based on a hidden Markov regime switching model and scenario generations. Firstly, we assume that investor’s risk preferences and beliefs are primarily observed in main world indices and then transmitted to other markets such as IInd pillar life-cycle pension funds. This enables both regime (market state) identification and regime-switching detection. Second, we apply stress to the transition matrix by increasing probabilities of moving to a worse market state, i.e., we increase the transition probabilities on the upper-right side of the main diagonal and decrease the diagonal probabilities correspondingly. Third, future scenarios (evolution) of returns of pension funds are generated using stressed transition matrices and basic risk and performance measures of the final wealth are presented. Finally, three different strategies for pension managers are considered and compared to each other. The results show how the best strategy depends on the stress level.

Multiple factor assessment for determining resting metabolic rate in young adults

Existing formulas cannot fully explain the variation of resting metabolic rate (RMR). This study aims to examine potential influencing factors beyond anthropometric measurements and develop more accurate equations using accessible parameters. 324 healthy adults (230 females; 18–32 years old) participated in the study. Height, fat-free mass (FFM), fat mass (FM) and RMR were measured. Menstrual cycle, stress levels, living habits, and frequency of consuming caffeinated foods were collected. Measured RMR were compared with predictive values of the new equations and previous 11 equations. Mean RMR for men and women was 1825.2 ± 248.8 and 1345.1 ± 178.7 kcal/day, respectively. RMR adjusted for FFM0.66FM0.066 was positively correlated with BMI. The multiple regression model showed that RMR can be predicted in this population with model 1 (with FFM, FM, age, sex and daily sun exposure duration) or model 2 (with weight and height replacing FFM and FM). The accuracy was 75.31% in the population for predictive model 1 and 70.68% for predictive model 2. The new equations had overall improved performance when compared with existing equations. The predictive formula that consider daily sun exposure duration improve RMR prediction in young adults. Additional investigation is required among individuals in the middle-aged and elderly demographic.

#1656 Ezetimibe improves diabetic nephropathy by upregulating Nrf2 to alleviate endoplasmic reticulum stress

Abstract Background and Aims Diabetic nephropathy (DN), a prevalent complication of diabetes mellitus, stands as the primary contributor to chronic kidney disease and end-stage renal disease. The progression of diabetic nephropathy is facilitated by endoplasmic reticulum stress and the excessive generation of reactive oxygen species (ROS). In addition to its cholesterol-lowering properties, recent research has revealed that ezetimibe also ameliorates insulin resistance and glucose intolerance abnormalities, and is also beneficial in chronic kidney disease. However, the specific impact of ezetimibe on diabetic nephropathy and the underlying mechanism remain unknown. Therefore, the objective of this research is to investigate the effect of ezetimibe on diabetic nephropathy and elucidate its mechanism. Method In vivo experiments were conducted using C57BL/6J mice, with a total of 24 mice randomly assigned to three groups: NC, DM, and EZE (10 mg/kg/day). In vitro experiments involved the treatment of human renal proximal tubular (HK-2) cells with ezetimibe (50 μM) and high glucose (30 mmol/L) for 48 hours. Then, combined with histopathological examination, biochemical evaluation, TUNEL stain, western blot analysis, Si-RNA transfection, transmission electron microscopy and ROS probe were used to detect the renal morphology and function, oxidative stress and endoplasmic reticulum homeostasis level. Results The results demonstrated that a 10-week treatment with ezetimibe led to significant improvements in urine volume, beta-N-acetylglucosaminidase (NAG), and the urinary albumin/creatinine ratio (ACR). The administration of ezetimibe in the treatment exhibited enhancements in glomerular hypertrophy and renal tubular vacuolization, as well as mesangial matrix expansion and glomerular basement membrane thickness, in diabetic mice. Additionally, ezetimibe treatment resulted in a reduction in ROS formation by upregulating the expression of antioxidant enzymes, such as superoxide dismutase (SOD) and catalase (CAT), through the activation of NF-E2-related factor-2 (Nrf2). Furthermore, ezetimibe administration partially reversed the increased expression of endoplasmic reticulum stress-related proteins, including activating transcription factor 6 (ATF6), glucose-regulated protein 78 (GRP78), and C/EBP homologous protein (CHOP), in DN mice. Ezetimibe demonstrated inhibitory effects on the accumulation of ROS and alleviated endoplasmic reticulum stress in HK-2 cells treated with high glucose. Furthermore, electron microscopy analysis revealed that ezetimibe effectively reduced the distension and dilation of the endoplasmic reticulum. However, the anti-endoplasmic reticulum stress effect of ezetimibe was significantly diminished following transfection with si-Nrf2. These findings suggest that ezetimibe exerts its anti-endoplasmic reticulum stress effect, at least in part, by enhancing antioxidant capacity and reducing ROS levels through the upregulation of Nrf2. Conclusion In summary, our research findings demonstrate the notable contribution of endoplasmic reticulum stress to the progression of DN. Through the up-regulation of Nrf2, ezetimibe effectively mitigates ROS generation in the context of DN, consequently ameliorating the adverse consequences of ERS, restoring equilibrium within the endoplasmic reticulum, and ultimately safeguarding against renal cell demise. Ezetimibe is an attractive potential therapy for the treatment of DN.

Mind-mindedness in mothers of infants with excessive crying/sleeping/eating disorders

IntroductionExcessive crying, sleeping, and eating disorders are among the most prevalent mental health diagnoses in the first 3 years of life and involve significant health service use. Parents of infants with excessive crying/sleeping/eating disorders report high levels of stress, since they feel incapable of soothing and/or nurturing their baby. Infants' distress can lead to a breakdown in parents' mentalizing abilities and, more specifically, parental mind-mindedness in the parent-child interaction. Moreover, the signals of infants with excessive crying/sleeping/eating disorders tend to be equivocal and difficult to read. This also might contribute to lower parent-child interaction quality. Until now, parental mind-mindedness, which is regarded as a prerequisite for sensitivity, has not been investigated in mothers of infants with excessive crying/sleeping/eating disorders. We investigated whether mind-mindedness in mothers of infants with excessive crying, sleeping and/or eating disorders differed from a healthy control group. We supposed that mothers of infants with excessive crying/sleeping/eating disorders would use (1) less appropriate mind-related comments (AMRCs), and (2) more non-attuned mind-related comments (NAMRCs) than mothers in the control group.MethodsOur sample consisted of 44 mothers and their infants who were patients in a socio-paediatric clinic in Germany. The children were diagnosed with excessive crying, sleeping and/or eating disorders according to DC:0-5 (= clinical group). The control group was composed of 64 healthy children and their mothers. Maternal mind-mindedness was coded during a free-play interaction.ResultsResults showed that mothers of infants with excessive crying, sleeping and/or eating disorders used both more AMRCs (p = .029) as well as more NAMRCs (p = .006) than mothers in the control group.DiscussionThe findings are discussed in terms of implications for interventions (e.g., enhancing mind-mindedness trough video-feedback).

High-impact chronic pain in sickle cell disease: insights from the Pain in Sickle Cell Epidemiology Study (PiSCES).

The US National Pain Strategy recommends identifying individuals with chronic pain (CP) who experience substantial restriction in work, social, or self-care activities as having high-impact chronic pain (HICP). High-impact chronic pain has not been examined among individuals with CP and sickle cell disease (SCD). We analyzed data from 63 individuals with SCD and CP who completed at least 5 months of pain diaries in the Pain in Sickle Cell Epidemiology Study (PiSCES). Forty-eight individuals met the definition for HICP, which was operationalized in this study as reporting pain interference on more than half of diary days. Compared with individuals without HICP, individuals with HICP experienced higher mean daily pain intensity, particularly on days without crises. They also experienced a greater proportion of days with pain, days with healthcare utilization, and days with home opioid use and higher levels of stress. They did not have a statistically significantly higher proportion of days with crises or experience higher mean daily pain intensity on days with crises. Individuals with HICP experienced worse physical functioning and worse physical health compared with those without HICP, controlling for mean pain intensity, age, sex, and education. The results of this study support that HICP is a severely affected subgroup of those with CP in SCD and is associated with greater pain burden and worse health outcomes. The findings from this study should be confirmed prospectively in a contemporary cohort of individuals with SCD.

Cornuside ameliorates cognitive impairments via RAGE/TXNIP/NF-κB signaling in Aβ1-42 induced Alzheimer's disease mice.

Cornuside has been discovered to improve learning and memory in AD mice, however, its underlying mechanism was not fully understood. In the present study, we established an AD mice model by intracerebroventricular injection of Aβ1-42, which were treated with cornuside (3, 10, 30mg/kg) for 2weeks. Cornuside significantly ameliorated cognitive function of AD mice in series of behavioral tests, including Morris water maze test, nest building test, novel object recognition test and step-down test. Additionally, cornuside could attenuate neuronal injury, and promote cholinergic synaptic transmission by restoring the level of acetylcholine (ACh) via inhibiting acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE), as well as facilitating choline acetyltransferase (ChAT). Furthermore, cornuside inhibited oxidative stress levels amplified as decreased malondialdehyde (MDA), by inhibiting TXNIP expression, improving total anti-oxidative capacity (TAOC), raising activities of superoxide dismutase (SOD) and catalase (CAT). Cornuside also reduced the activation of microglia and astrocytes, decreased the level of proinflammatory factors TNF-α, IL-6, IL-1β, iNOS and COX2 via interfering RAGE-mediated IKK-IκB-NF-κB phosphorylation. Similar anti-oxidative and anti-inflammatory effects were also found in LPS-stimulated BV2 cells via hampering RAGE-mediated TXNIP activation and NF-κB nuclear translocation. Virtual docking revealed that cornuside could interact with the active pocket of RAGE V domain directly. In conclusion, cornuside could bind to the RAGE directly impeding the interaction of Aβ and RAGE, and cut down the expression of TXNIP inhibiting ROS production and oxidative stress, as well as hamper NF-κB p65 mediated the inflammation.

Gut Microbiome-Mediated Mechanisms in Alleviating Opioid Addiction with Aqueous Extract of Anacyclus pyrethrum

The escalating rates of morbidity and mortality associated with opioid use disorder (OUD) have spurred a critical need for improved treatment outcomes. This study aimed to investigate the impact of prolonged exposure to Fentanyl, a potent opioid, on behavior, biochemical markers, oxidative stress, and the composition of the gut microbiome. Additionally, we sought to explore the therapeutic potential of Anacyclus pyrethrum in mitigating the adverse effects of Fentanyl withdrawal. The study unveiled that chronic Fentanyl administration induced a withdrawal syndrome characterized by elevated cortisol levels (12.09 mg/mL, compared to 6.3 mg/mL for the control group). This was accompanied by heightened anxiety, indicated by a reduction in time spent and entries made into the open arm in the Elevated Plus Maze Test, as well as depressive-like behaviors, manifested through increased immobility time in the Forced Swim Test. Additionally, Fentanyl exposure correlated with decreased gut microbiome density and diversity, coupled with heightened oxidative stress levels, evidenced by elevated malondialdehyde (MDA) and reduced levels of catalase (CAT) and superoxide dismutase (SOD). However, both post- and co-administration of A. pyrethrum exhibited substantial improvements in these adverse effects, effectively alleviating symptoms associated with OUD withdrawal syndrome and eliciting positive influences on gut microbiota. In conclusion, this research underscores the therapeutic potential of A. pyrethrum in managing Fentanyl withdrawal symptoms. The findings indicate promising effects in alleviating behavioral impairments, reducing stress, restoring gut microbiota, and mitigating oxidative stress, offering valuable insights for addressing the challenges of OUD treatment.