Stress Tolerance Research Articles

Screening of Psidium Species and Interspecific Hybrid Progenies for Salinity Stress Tolerance

Screening of Psidium Species and Interspecific Hybrid Progenies for Salinity Stress Tolerance

Ectopic expression of HvbHLH132 from hulless barley reduces cold tolerance in transgenic Arabidopsis thaliana.

Overexpression of HvbHLH132 from hulless barley impairs in chilling and freezing tolerance at the seedlings stage in Arabidopsis thaliana The basic helix-loop-helix (bHLH) transcription factors (TF) are ubiquitously existed in eukaryote and play crucial roles in numerous biological processes. However, the characterization of their members and functions in hulless barley remains limited. Here, we conducted a genome-wide identification of the HvbHLH gene family and assessed the role of HvbHLH132 in cold stress tolerance. We identified 141 HvbHLH genes, which were categorized into twelve subfamilies. Subcellular localization predictions indicated that the majority of HvbHLH proteins were localized in the nucleus. cis-Acting element analysis revealed that the promoter regions of the HvbHLH family contain diverse elements associated with various biological processes. Expression profiling of the 141 HvbHLH genes in two extreme varieties revealed that HvbHLH132 was significantly induced and exhibited substantial differential expression under cold stress. Analyses of subcellular localization and transactivation activity confirmed that HvbHLH132 specifically localized in the nucleus and contributed to transcriptional activation. Furthermore, overexpression of HvbHLH132 in Arabidopsis resulted in impaired chilling and freezing tolerance at the seedling stage, leading to biochemical changes unfavorable for freezing stress. Additionally, the expression of some cold-responsive genes (COR) genes was significantly less induced compared to wild type under freezing stress. This study provides comprehensive insight into the HvbHLH gene family and reveals a critical role of HvbHLH132 in regulating cold tolerance in plants.

The Impact of Individual and Team‐Level Variables on Burnout in Healthcare Providers

ABSTRACTThis study evaluates the relationships between individual and team‐level factors in influencing burnout among clinical healthcare providers. Focusing on psychological safety, perceived autonomy, perceived team effectiveness, and emotional intelligence, the research aims to understand how these elements contribute to the prevalence and severity of burnout symptoms. Using electronic questionnaires analyzed through Confirmatory Factor Analysis (CFA) and Structural Equation Modeling (SEM), the study sampled 180 healthcare providers from one large US medical center. The study results found that psychological safety significantly decreases levels of burnout, particularly emotional exhaustion. The results on team effectiveness suggest a complex relationship with burnout, with different dimensions having varied impacts. The study did not find support for the negative prediction of burnout by perceived autonomy and emotional intelligence, contrary to expectations based on prior research. These findings have practical implications for healthcare management, stressing the importance of psychological safety and effective team dynamics in reducing burnout. Overall, this study contributes significantly to understanding burnout in healthcare, emphasizing the critical role of team structures and individual emotional resilience in managing workplace well‐being.

Stress-Easing Effect of Diacyl Glyceryl Ethers on Anxiety-Related Behavior in Mice

Stress and anxiety are significant psychological challenges in modern society, which have led to a rapidly growing market for functional foods, including those reported to relieve stress, as alternatives to psychoactive drugs. Among these, diacyl glyceryl ethers (DAGE) derived from deep-sea shark liver oil have gained attention for their strong antioxidant properties and potential mental health benefits. Building on preliminary evidence suggesting DAGE’s efficacy in enhancing stress resilience and modulating biochemical pathways associated with reduced oxidative stress, the present study aimed to examine their effects on stress responses in two specific mouse strains. Each mouse was fed either a DAGE-infused diet or a control diet for three weeks. Their stress responses were evaluated using three behavioral tests: the elevated plus maze, open-field, and forced swimming tests. The DAGE-fed mice displayed lower stress responses than the control mice in the initial trial of each test. Specifically, DAGE-fed mice demonstrated increased time spent in the open arms in the elevated plus maze and more time spent in the center of the open field, suggesting reduced anxiety. Additionally, in the forced swimming test, DAGE-treated mice displayed reduced immobility times, indicating potential antidepressant effects on the mice. These findings suggest the potential of DAGE to bolster stress resilience in mice, emphasizing their promise for further studies in human stress management.

Psychological resilience and its relationship to coping with psychological stress strategies among a sample of battered women who benefited from a single service or multi-sectoral services in the North Governorate

Psychological resilience and its relationship to coping with psychological stress strategies among a sample of battered women who benefited from a single service or multi-sectoral services in the North Governorate

Promoting Lifelong Learning in Arts and Health

This paper examines the intersection of lifelong learning with the arts and health, focusing on how continuous education in these areas’ benefits individuals and society. Lifelong learning in arts and health not only promotes personal development and professional growth but also fosters well-being, emotional resilience, and social connectivity. The arts can be a critical tool in health care, improving mental health outcomes and fostering creativity among practitioners. Despite these benefits, barriers such as funding, time constraints, and lack of resources hinder the integration of arts into health education and practice. These study outlines strategies for overcoming these challenges through innovative pedagogical approaches, digital learning, and policy initiatives. It emphasizes the importance of creating environments that support both formal and informal learning, enabling broader participation in arts-based health interventions. Case studies and best practices illustrate successful models of lifelong learning in arts and health, demonstrating their potential to reduce social exclusion and enhance the quality of care. Keywords: Lifelong learning, arts in health, creativity, well-being, health education, community arts, professional development.

The Role of Psychological Factors and Resilience in Mediating Sports Passion in Triathletes

The present study aims to analyze the psychological characteristics associated with the performance of amateur athletes, exploring their relationships with key variables such as resilience, harmonious and obsessive passion, and perception of discomfort. The main objective is to establish the relationship between psychological characteristics and each of the variables used (resilience, personal competence, and acceptance of self and life), and to consider whether these are related to passion (harmonious and obsessive) through a structural equation analysis. The sample was composed of 110 persons: 87 adults (22 females, mean age 40.7 ± 9.1 years; 65 males, mean age in years 42.1 ± 11.9) and 23 adolescents (16 females, mean age 14.8 ± 1.3 years; 7 males, mean age 15.3 ± 2.1 years). Psychological characteristics were assessed using the Psychological Characteristics Related to Performance (CPRD) questionnaire, passion using the Passion Scale instrument, and resilience using the Resilience Scale (RS-14) questionnaire. The analyses were based on a structural equation model analysis (PLS-SEM). The results showed adequate coefficients of determination (R index2) and Stone–Geisser predictive relevance (Q2) for the personal competence resilience factors (R2 = 0.517; Q2 = 0.218) and acceptance of self and life (R2 =.415; Q2 = 0.231), as well as for the passion dimensions harmonious passion (R2 = 0.357; Q2 = 0.168) and obsessive passion (R2 = 0.085; Q2 = 0.034). In conclusion, a close relationship was demonstrated between psychological characteristics related to sports performance and the variables of resilience and passion (both harmonious and obsessive). In particular, it was confirmed that mental skills and stress management are linked to the dimensions of resilience, and these dimensions influence both types of passion. In addition, team cohesion and personal competence also play a crucial role in the development of passion, highlighting the importance of these factors in the assessment of sports performance and influencing the well-being of amateur athletes in their personal and sports development.

The RALF1-FERONIA complex phosphorylates FREE1 to attenuate abscisic acid signaling during seedling establishment.

The receptor-like kinase FERONIA (FER), together with its ligand rapid alkalinization factor 1 (RALF1) peptide, plays a crucial role in regulating stress responses, including its involvement in modulating abscisic acid (ABA) signaling. FER has been shown to activate ABA Insensitive 2 (ABI2) in the cytoplasm, leading to the suppression of ABA signaling. However, its regulation of nucleus events in the ABA response remains unclear. FREE1, identified as a plant-specific component of the endosomal sorting complex required for transport (ESCRT) in eukaryotes, serves as an important negative regulator in ABA signaling. In this study, we elucidate that upon RALF1 treatment, FER phosphorylates FREE1, promoting the accumulation of FREE1 protein in the nucleus in Arabidopsis (Arabidopsis thaliana). Consequently, FREE1 suppresses ABA sensitivity by inhibiting the expression of ABA-response genes. Mutating the six identified phosphorylation sites on FREE1, mediated by FER, to non-phosphorylable residues results in reduced nucleus localization of FREE1 and increased hypersensitivity to ABA. Our data also show that these six phosphorylation sites are likely involved in regulating plant survival under salt stress. Collectively, our study not only unveils an additional function of FER in attenuating ABA signaling in the nucleus but also provides a possible insight into the role of the RALF1-FER-FREE1 module in coordinating plant growth and salt stress tolerance.

Polyamine Seed Priming: A Way to Enhance Stress Tolerance in Plants

Polyamines (PAs), such as putrescine, spermine, and spermidine, are bioactive molecules that play a vital role in plant responses to stresses. Although they are frequently applied to achieve higher levels of stress tolerance in plants, their function in seed biology is still not fully understood. PAs have been described in only a limited number of studies as seed priming agents, but most of the data report only the physiological and biochemical PA effects, and only a few reports concern the molecular mechanisms. In this review, we summarized PA seed priming effects on germination, seedling establishment, and young plant response to abiotic stresses, and tried to draw a general scheme of PA action during early developmental plant stages.

Engineering cold resilience: implementing gene editing tools for plant cold stress tolerance.

This paper highlights the need for innovative approaches to enhance cold tolerance. It underscores how genome-editing tools can deepen our understanding of genes involved in cold stress. Cold stress is a significant abiotic factor in high-altitude regions, adversely affecting plant growth and limiting crop productivity. Plants have evolved various mechanisms in response to low temperatures that enable resistance at both physiological and molecular levels during chilling and freezing stress. Several cold-inducible genes have been isolated and characterized, with most playing key roles in providing tolerance against low-temperature stress. However, many plants fail to survive at low temperatures due to the absence of cold acclimatization mechanisms. Conventional breeding techniques, such as inter-specific or inter-genic hybridization, have had limited effectiveness in enhancing the cold resistance of essential crops. Thus, it is crucial to develop crops with improved adaptability, high yields and resistance to cold stress using advanced genomic approaches. The current availability of gene editing tools offers the opportunity to introduce targeted modifications in plant genomes efficiently, thereby developing cold-tolerant varieties. This review discusses advancements in gene editing tools, including zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)/Cas12a(Cpf1), prime editing (PE) and retron library recombineering (RLR). We focus specifically on the CRISPR/Cas system, which has garnered significant attention in recent years as a groundbreaking tool for genome editing across various species. These techniques will enhance our understanding of molecular interactions under low-temperature stress response and highlight the progress of genome editing in designing future climate-resilient crops.

A C2H2-type zinc finger protein TaZFP8-5B negatively regulates disease resistance

BackgroundZinc finger proteins (ZFPs) are important regulators in abiotic and biotic stress tolerance in plants. However, the role of the ZFPs in wheat responding to pathogen infection is poorly understood.ResultsIn this study, we found TaZFP8-5B was down-regulated by Puccinia striiformis f. sp. tritici (Pst) infection. TaZFP8-5B possesses a single C2H2-type zinc finger domain with a plant-specific QALGGH motif, and an EAR motif (LxLxL) at the C-terminus. The EAR motif represses the trans-activation ability of TaZFP8-5B. Knocking down the expression of TaZFP8 by virus-induced gene silencing increased wheat resistance to Pst, whereas TaZFP8-5B-overexpressing reduced wheat resistance to stripe rust and rice resistance to Magnaporthe oryzae, suggesting that TaZFP8-5B plays a negative role in the modulation of plant immunity. Using bimolecular fluorescence complementation, split-luciferase, and yeast two-hybrid assays, we showed that TaZFP8-5B interacted with a wheat calmodulin-like protein TaCML21. Knock-down of TaCML21 reduced wheat resistance to Pst.ConclusionsThis study characterized the function of TaZFP8-5B and its interacting protein TaCML21. Our findings provide a new perspective on a regulatory module made up of TaCML21-TaZFP8-5B in plant immunity.

Deciphering the ABA and GA biosynthesis approach of Bacillus pumilus, mechanistic approach, explaining the role of metabolic region as an aid in improving the stress tolerance

Bacillus pumilus plays an essential role in agricultural applications as a beneficial microbe and for sustainable agriculture production. However, the underlying mechanisms of B. pumilus strains remain unclear as to how they are beneficial for plants as stress tolerant and growth promoters. Bacillus pumilus was isolated from the rhizosphere soil of Artemisia vulgaris. NGS (next-generation sequencing) was performed for the strain to gain new insights into the molecular mechanisms underlying plant-microbial interactions. NGS revealed 3,910 genes, 3294 genes with protein-coding, and 11 functional genomic regions related to diverse agronomic traits including stress tolerance. We identified the two possible phytohormone biosynthesis approaches from metabolic regions1(terpense→diterpense→betacarotene→xanthoxin→ABA)2(terpense→diterpense→geranyl diphosphate →C20 →GA). Several gene clusters related to the biosynthesis of phytohormones, stress tolerance, and agricultural diversification were predicted. The genome provides insights into the possible mechanisms of this bacterium for stress tolerance and its future applications. The genomic organization of B. pumilus revealed several hallmarks of its plant growth promotion and pathogen suppression activities. Our results provide detailed genomic information for the strain and reveal its potential stress tolerance mechanisms, laying the foundation for developing effective stress tolerance strategies against abiotic stress.

Molecular and functional adaption of Arabidopsis villins.

Villins are versatile, multifunctional actin regulatory proteins. They promote actin stabilization and remodeling mainly via their actin bundling and Ca2+-dependent severing activities, respectively. Arabidopsis subclass II and III villins normally coexist in cells, but the biological significance of their coexistence remains unknown. Here we demonstrate that subclass II villin binds to Ca2+ with high affinity and exhibits strong severing but weak bundling activity compared to subclass III villin. Subclass II villin plays a dominant role in promoting actin remodeling, which requires its Ca2+-dependent severing activity. Subclass II villin is also strictly required for physiological processes including oriented organ growth and stress tolerance. By comparison, subclass III villin binds to Ca2+ with low affinity and exhibits weak severing but strong bundling activity, and acts as the major player in controlling actin stabilization and organization. Thus, we demonstrate that multifunctional villin isovariants have diverged biochemically to ensure exquisite control of the actin cytoskeleton to meet different cellular needs in plants. This study provides new insights into the role of villins in fine-tuning actin dynamics and plant development.

The role of mobile applications and online platforms in the rehabilitation process of patients with coronary heart disease: a literature review

IntroductionNowadays, diseases of the circulatory system, such as coronary heart disease, are among the leading causes of death worldwide. Cardiac rehabilitation, a complex, interdisciplinary intervention, aims to improve cardiovascular function, enhance quality of life, and reduce mortality risk. In the era of mobile technology development, mobile applications and online platforms supporting cardiac rehabilitation are gaining increasing importance. Aim of the study The aim of this study is to review the literature on the effectiveness of digital tools as support mechanisms in the rehabilitation of patients with coronary heart disease, and to compare their effectiveness with traditional medical care. Materials and MethodsA review of randomized clinical trials (RCTs) published in 2024 regarding the use of mobile and online applications in cardiac rehabilitation was conducted. Five studies meeting specific selection criteria were identified. Results Research shows that mobile applications and online platforms effectively support the rehabilitation of patients with coronary artery disease, achieving results comparable to or better than traditional care. Programs such as RehaPlus+ and mHealth improve physical activity, mental resilience, quality of life, and reduce stress and anxiety. Users of applications such as eMOTIVA and MCARE achieve better results in terms of diet, physical activity, and general well-being. These interventions also promote regular communication with the therapist, which supports the individualization of rehabilitation. Conclusions Rehabilitation of patients with coronary heart disease using mobile applications or online platforms is equally effective as, or more effective than, standard medical care.

Assessing Salinity, Drought and High Temperature Stress in Maize (Zea mays L.) and Wheat (Triticum aestivum L.) Varieties: Theoretical Combination as Multifactorial Stress

ABSTRACTMaize and wheat are two important cereal crops for the food security of the world population. However, constant climate change and the intensification of anthropic activities have intensified the emergence of stressful environmental in the various agricultural production systems around the world. Therefore, in this study we evaluate the chlorophyll content, photosynthesis, transpiration and grain yield of maize and wheat crops exposed to soil salinity, drought and high temperatures and determine the damage intensity of these stressing conditions and the theoretical multifactorial damage intensity. Field experiments were conducted during the 2022 and 2023 agricultural seasons in the Yaqui Valley, Sonora, Mexico. The treatments consisted of the cultivation of maize and wheat in three stressful production environments (soil salinity, drought and high temperatures) and a non‐stressful production environment (Control), with four repetitions. The tolerance and intensity index of abiotic stresses, as well as the intensity of theoretical multifactorial stress (salinity, drought and high temperatures), for morphological traits and grain yield, were calculated. The results reported that physiological traits and yield of maize and wheat are severely affected by drought stress conditions. High temperatures are the second abiotic stress factor that most limits physiological traits and grain yield of maize and wheat crops, being more harmful than soil salinity. The theoretical multifactorial stress has a greater negative impact on the yield of the elite maize and wheat varieties. The sum of a stressful environmental factor increases the intensity of multifactorial stress on grain yield of both cereal crops, especially for maize crop.

Music and Adolescent Mental Health: A Journey of Healing, Growth, and Self-Discovery

Music, as an emotional expression and adjustment tool, has a significant impact on the mental health of teenagers. Through a four-week experiment, this study explored the influence of music on teenagers' emotional state, psychological resilience, anxiety level, self-cognition and life satisfaction. The subjects were divided into experimental group and control group. The experimental group listened to healing music for 30 minutes every day, while the control group conducted quiet meditation activities. The mental health status of the two groups of teenagers was measured and compared by psychological scales such as PANAS, RS, STAI, self-concept and life satisfaction. The results show that the experimental group is significantly better than the control group in emotion regulation, psychological resilience improvement and self-cognition, which proves that music has the potential to promote the mental health of teenagers. This paper discusses the theoretical and practical significance of these findings.

Evaluating Drought Tolerance and Yield Stability of Sorghum Genotypes for Sustainable Agriculture in Sohag, Egypt

ABSTRACTThe major problem in the cultivation of sorghum in the Egyptian agricultural system includes arid climate and water shortage. Thus, the present study was undertaken to investigate the effect of drought conditions on the productivity of 21 different sorghum (Sorghum bicolor L.) genotypes at Sohag, Egypt. A set of drought tolerance metrics employed in the present study were Tolerance Index (TOL); Mean Production (MP); Sensitivity to Drought Index (SSI); Stress Tolerance Index (STI); Geometric mean performance (GMP) and Harmonic mean of yield (HARM). A field‐controlled experiment was conducted on the two growth seasons (2021 and 2022) concerning the impact of different drought levels on (ET0 = 0.8, 0.6 and 0.4) Grain Yields (GY) and Water Use Efficiency (WUE) for the various studied sorghum genotypes. Principal Component Analysis (PCA) was also conducted on the obtained data of the different yield parameters with the aim of identifying the most effective tolerance indices related to the different genotypes of sorghum under both conditions, optimal and stressed. Drought tolerance varies among different sorghum varieties, which, by their genetic and physiological nature, possess efficient stomatal regulation and deep‐rooted systems, enhancing water conservation and physiological functions and hence higher WUE. These are mainly influenced by environmental conditions, such as soil type and moisture levels in the region of Sohag, where such genotypes may exhibit different performances regarding drought stress. It was observed that Giza‐15 and Hybrid Sh1 were among the high performers under well‐irrigated conditions, and L38 was outstanding during a severe drought in terms of WUE. Therefore, proper genotype selection depends on the irrigation strategy. Hence, the Sohag region presents a good opportunity for further improvement of drought tolerance through designed selection and hybridization activities in sorghum breeders, and this contributes to climate‐resilient cultivars by addressing the present agricultural challenges and food security for the present world. Emphasis will be placed by breeders on the development of arid‐condition‐adapted genotypes and the development of models that can be applied within comparable climates to boost productivity and sustainability for those farmers dependent on this vital crop.

Early-life cold stress and broiler breeder age: effects on metabolism and performance in broiler chicks

Context Understanding the interaction between breeder age and environmental stressors, such as cold, is crucial for poultry production. Previous research has highlighted the impact of these factors on chick development, but there is limited information on how breeder age specifically influences stress resilience and growth performance in broilers, particularly under suboptimal conditions. Aims The study aimed to assess the impact of broiler breeder age and 8-h exposure to cold stress on chicks during the first 7 days of rearing. Methods A total of 600 male chicks from two Cobb flocks were divided into groups based on breeder age (29 or 42 weeks) and rearing temperature (thermoneutral or cold-stressed for 8 h/day during the first week). Key results Chicks from 42-week-old breeders exhibited faster yolk sac absorption under thermal comfort conditions (P < 0.05). Corticosterone levels were higher in chicks from 29-week-old breeders exposed to cold stress (P < 0.05). Cold-exposed birds showed elevated heterophil/lymphocyte ratios regardless of breeders’ age (P < 0.05). Feed intake and weight gain were superior in birds from 42-week-old breeders up to 35 days, whereas cold stress negatively impacted the feed conversion ratio (P < 0.05). Viability was lower in chicks from 29-week-old breeders and those subjected to cold stress (P < 0.05). Conclusion Although cold stress did not affect weight gain at 35 days, it impaired the feed conversion ratio. Moreover, chicks from 29-week-old breeders were more susceptible to cold stress during the first week of life compared with those from 42-week-old breeders. Implications The study reveals that breeder age significantly influences chick resilience to cold stress, with older breeders (42-week-old) producing more robust offspring. The findings suggest that optimizing the management of thermal conditions is critical for enhancing feed efficiency, growth performance and overall viability in broiler production.

Genome-Wide Identification of the PAP Gene Family in Potatoes and Its Expression Response to Phosphorus-Solubilizing Bacteria, Freezing Stresses, and Phytohormonal Regulation

Purple acid phosphatases (PAPs) are multifunctional proteins implicated in various aspects of plant biology, including flowering, seed development, senescence, carbon metabolism, and the response to biotic and abiotic stresses. Although PAPs have been reported in potatoes, the gene features and expression under phosphorus-solubilizing bacteria and freezing stresses remain unclear. In the present study, we identified 29 StPAPs with molecular weights ranging from 27.96 to 85.40 kDa, which were classified into three distinct groups based on a phylogenetic analysis. Integrating gene chromosomal localization, gene duplication events, intergenic collinearity, and gene selection pressure analysis, we found that the expansion of the potato PAP gene family was primarily driven by genome-wide duplication events, with the majority of the PAP genes undergoing purifying selection. Promoter cis-acting element analysis and RNA-Seq data revealed that a total of 89 cis-acting elements were associated with plant hormones, and the seven StPAP genes for low phosphorous response were associated with responses to abscisic acid, gibberellin, and abiotic stresses. Tissue expression pattern analyses indicated that the expression of StPAP genes were regulated in response to varying phosphorus levels. A co-expression network analysis identified eight StPAP genes involved in symbiosis with phosphorus-solubilizing bacteria, and seven StPAP genes exhibited significantly higher expression in response to freezing stress and abiotic stresses (drought, heat, darkness, and N/K/Ca/Fe/Mg/Zn deficiencies). In conclusion, the StPAP may synergistically modulate phytohormone levels in response to stress by regulating plant phosphorus homeostasis; StPAP12 and abscisic acid are key phosphorus-regulated genes and phytohormones in response to freezing stress. This study represents the first report to demonstrating the responsiveness of StPAPs to phytohormones, revealing a potential new function for these genes in plant stress responses and nutrient management. These findings provide novel insights into the multifaceted roles of PAPs in plant adaptation and stress tolerance.

Role of Hog1-mediated stress tolerance in biofilm formation by the pathogenic fungus Trichosporon asahii

Trichosporon asahii, a dimorphic fungus, causes bloodstream infections in immunocompromised patients with neutropenia. Biofilms are formed on the surfaces of medical devices such as catheters as T. asahii transitions morphologically from yeast to hyphae in the host environment. Oxidative stress tolerance and morphological changes of T. asahii are regulated by Hog1, a mitogen-activated protein kinase. The role of Hog1 in the biofilm formation by T. asahii, however, has remained unknown. In the present study, we demonstrated that a hog1 gene-deficient T. asahii mutant formed excess biofilm under a rich medium in vitro, but did not form biofilm in an in vivo evaluation system using silkworms. The hog1 gene-deficient T. asahii mutant formed a greater amount of biofilm than the parent strain in vitro. Under an oxidative stress condition in vitro, however, lower amounts of biofilm were formed by the hog1 gene-deficient T. asahii mutant than by the parent strain. In an in vivo evaluation system using silkworms, lower amounts of biofilm were formed by the hog1 gene-deficient T. asahii mutant than by the parent strain. Our findings suggest that Hog1 regulates biofilm formation by T. asahii in response to host environmental conditions, including oxidative stress.