Stress In Regions Research Articles

Effects of halotolerant rhizobacteria on rice seedlings under salinity stress

Avirulent halotolerant plant growth-promoting rhizobacteria (HPGPR) located on the roots' periphery can reduce abiotic stressors (such as salinity and drought), enhance plant productivity. Salinity poses a significant challenge for growing agricultural products, like rice, in the coastal regions. It is crucial to enhance production because of limited arable land and the high growth rate of the population. This study targeted to identify HPGPR from legume root nodules and assessed their effects on rice plants experiencing salt stress in coastal regions of Bangladesh. Based on the culture morphology, biochemical, salt, pH, and temperature tolerance traits, sixteen bacteria were isolated from the root nodules of leguminous plants (Common bean, Yardlong bean, Dhaincha, and Shameplant). All the bacterial strains can tolerate 3 % salt concentration, and capable to survive at the highest 45 °C temperature and pH 11 (without isolate 1). Three preeminent bacteria, Agrobacterium tumefaciens (B1), Bacillus subtilis (B2), and Lysinibacillus fusiformis (B3) were specified through morpho-biochemical and molecular (16S rRNA gene sequence) exploration for inoculation. To assess the plant growth-promoting activities, germination tests are applied where bacterial inoculation increased germination in saline and non-saline conditions. Control group (C) showed 89.47 % and bacterial treated groups (C + B1, C + B2, and C + B3) 95 %, 90 %, and 75 % germination after 2 days of inoculation. In (1 % NaCl) saline condition control group revealed 40 % whereas three groups with bacteria showed 60 %, 40 %, and 70 % germination after 3 days, which increased 70 %, 90 %, 85 %, and 95 % respectively after 4 days of inoculation. The HPGPR significantly improved plant development metrics such as root length, shoot length, fresh and arid biomass yield, chlorophyll content, etc. Our results suggest that the salt-resistant bacteria (Halotolerant) have a great potential role in recuperating plant growth and would be cost-effective as a bio-inoculant in saline conditions to be used as a prospective bio-fertilizer for rice production. These findings indicate that the HPGPR has a substantially promising function in reviving plant development in an eco-friendly manner.

Biomechanics of calcaneus impacted by talus: a dynamic finite element analysis

This paper aimed to investigate the biomechanical changes during the talus impact with the calcaneus at varying velocities. Various three-dimensional reconstruction software was utilized to construct a finite element model that consisted of the talus, calcaneus, and ligaments. The explicit dynamics method was used to explore the process of the talus impacting on the calcaneus. The velocity of impact was altered from 5 m/s to 10 m/s with a 1 m/s interval. Stress readings were collected from the posterior, intermediate, and anterior subtalar articular (PSA, ISA, ASA), calcaneocubic articular (CA), Gissane Angle (GA), calcaneal base (BC), medial wall (MW), and lateral wall (LW) of the calcaneus. The changes in the amount and distribution of stress in the different regions of the calcaneus that varied with velocity were analysed. The model was validated through comparison with findings from the existing literature. During the process of impact between the talus and calcaneus, the stress in the PSA reached its peak first. Notably, stress was concentrated mainly in the PSA, ASA, MW, and LW of the calcaneus. At varying impact velocities of the talus, the mean maximum stress of the PSA, LW, CA, BA, and MW exhibited statistically significant differences (P values were 0.024, 0.004, <0.001, <0.001, and 0.001, respectively). However, the mean maximum stress of the ISA, ASA, and GA was not statistically significant (P values were 0.289, 0.213, and 0.087, respectively). In comparison with the velocity at 5 m/s, the mean maximum stress increases in each region of the calcaneus at a velocity of 10 m/s were as follows: PSA 73.81%, ISA 7.11%, ASA 63.57%, GA 89.10%, LW 140.16%, CA 140.58%, BC 137.67%, MW 135.99%. The regions of stress concentration were altered, and the magnitude and sequence of peak stress in the calcaneus also varied according to the velocity of the talus during impact. In conclusion, the velocity of the talus during impact had a significant influence on the magnitude and distribution of stress within the calcaneus, which was a crucial factor in the development of calcaneal fractures. It was possible that the magnitude and sequence of stress peaks played a vital role in determining the emergence of fracture patterns.

Seed priming with plant growth-promoting bacteria (PGPB) improves growth and water stress tolerance of Secalemontanum

Abiotic and biotic stresses are major global threats to food security in the 21st century. Application of plant growth-promoting bacteria (PGPB) in rangeland plants is the only possible alternative that supports plant growth and development to combat environmental stress and successfully restoring rangelands. PGPBs were also found to be a potential substitute for chemical fertilizers and pesticides. The challenge is to determine which biofertilizers can be used for Secale montanum in normal and under water stress conditions. We sought to determine the benefits of PGPB for S. montanum under water stress conditions in terms of seedling growth traits, growth indicators, and nutrient uptake in the research greenhouse. Therefore, a completely randomized factorial design was conducted with two treatments of PGPB inoculation, including the control (no PGPB inoculation), PGPBs Bacillus cereus, Pseudomonas aeruginosa, Azospirillum lipoferm, and Azotobacter chroococcum, and water stress in the research greenhouse. Overall, the results of the current study showed that water stress greatly reduced the above-ground fresh weight of above-ground plant parts and the nitrogen and potassium content of S. montanum. The present study confirms the positive effects of PGPB on fresh and dry weights of above- and below-ground parts and seedling, vigor index, quality index, and nitrogen and potassium content of S. montanum, except for below-ground parts length, compared with the controls, which shows that PGPB usually improves some indicators of plant growth and development. We suggest that restoration of S. montanum seed inoculation with PGPB should be supported in degraded rangelands and marginal drylands in low rainfall years, which may cause water scarcity and consequently water stress in arid and semi-arid regions.

LMP2 deficiency causes abnormal metabolism, oxidative stress, neuroinflammation, myelin loss and neurobehavioral dysfunctions

BackgroundSubstantial evidence suggests that immunoproteasome is implicated in the various neurological diseases such as stroke, multiple sclerosis and neurodegenerative diseases. However, whether the immunoproteasome itself deficiency causes brain disease is still unclear. Therefore, the aim of this study was to explore the contribution of the immunoproteasome subunit low molecular weight protein 2 (LMP2) in neurobehavioral functions.MethodsMale LMP2 gene completed knockout (LMP2-KO) and littermate wild type (WT) Sprague–Dawley (SD) rats aged 12-month-old were used for neurobehavioral testing and detection of proteins expression by western blotting and immunofluorescence. A battery of neurobehavioral test tools including Morris water maze (MWM), open field maze, elevated plus maze were used to evaluate the neurobehavioral changes in rats. Evans blue (EB) assay, Luxol fast blue (LFB) and Dihydroethidium (DHE) staining were applied to explore the blood–brain barrier (BBB) integrity, brain myelin damage and brain intracellular reactive oxygen species (ROS) levels, respectively.ResultsWe firstly found that LMP2 gene deletion did not cause significantly difference in rats’ daily feeding activity, growth and development as well as blood routine, but it led to metabolic abnormalities including higher levels of low-density lipoprotein cholesterol, uric acid and blood glucose in the LMP2-KO rats. Compared with the WT rats, LMP2-KO rats displayed obviously cognitive impairment and decreased exploratory activities, increased anxiety-like behavior and without strong effects on gross locomotor abilities. Furthermore, multiple myelin loss, increased BBB leakage, downregulation of tight junction proteins ZO-1, claudin-5 and occluding, and enhanced amyloid-β protein deposition were observed in brain regions of LMP2-KO rats. In addition, LMP2 deficiency significantly enhanced oxidative stress with elevated levels of ROS, caused the reactivation of astrocytes and microglials and markedly upregulated protein expression levels of interleukin (IL)-1 receptor-associated kinase 1 (IRAK1), IL-6 and tumor necrosis factor-α (TNF-α) compared to the WT rats, respectively.ConclusionThese findings highlight LMP2 gene global deletion causes significant neurobehavioral dysfunctions. All these factors including metabolic abnormalities, multiple myelin loss, elevated levels of ROS, increased BBB leakage and enhanced amyloid-β protein deposition maybe work together and eventually led to chronic oxidative stress and neuroinflammation response in the brain regions of LMP2-KO rats, which contributed to the initial and progress of cognitive impairment.

Uptake Routes and Biodistribution of Polystyrene Nanoplastics on Zebrafish Larvae and Toxic Effects on Development

Polystyrene (PS) is the most widely used plastic polymer. It is mainly used to produce disposable products. Due to its resistance to degradation, PS can remain in the environment for a long time. Its mechanical, physical and biological actions determine the release of smaller fragments, which are able to penetrate organisms and accumulate in target organs. Fertilized Danio rerio eggs were exposed to concentrations of 10 and 20 mg/L of fluorescent, amino-modified polystyrene nanoplastics (nPS-NH2) with diameters of 100 and 50 nm for 96h, according to OECD guidelines (2013). Uptake, biodistribution, toxicity, oxidative stress and apoptosis were evaluated; moreover, we carried out a simulation to study the interactions between nPS-NH2 and defined regions of three receptors: STRA6, Adgrg6 and CNTN4/APLP2. We demonstrated that after being internalized, nPS-NH2 could reach the head and bioaccumulate, especially in the eyes. Moreover, they could lead to oxidative stress and apoptosis in the several regions where they bioaccumulated due to their interaction with receptors. This study confirmed the danger of nanoplastic wastes released in the environment.

Osmoregulatory and Antioxidants Modulation by Salicylic Acid and Methionine in Cowpea Plants under the Water Restriction.

Global climate changes have intensified water stress in arid and semi-arid regions, reducing plant growth and yield. In this scenario, the present study aimed to evaluate the mitigating action of salicylic acid and methionine in cowpea cultivars under water restriction conditions. An experiment was conducted in a completely randomized design with treatments set up in a 2 × 5 factorial arrangement corresponding to two cowpea cultivars (BRS Novaera and BRS Pajeú) and five treatments of water replenishment, salicylic acid, and methionine. After eight days, water stress decreased the Ψw, leaf area, and fresh mass and increased the total soluble sugars and catalase activity in the two cultivars. After sixteen days, water stress increased the activity of the superoxide dismutase and ascorbate peroxidase enzymes and decreased the total soluble sugars content and catalase activity of BRS Pajeú plants. This stress response was intensified in the BRS Pajeú plants sprayed with salicylic acid and the BRS Novaera plants with salicylic acid or methionine. BRS Pajeú is more tolerant to water stress than BRS Novaera; therefore, the regulations induced by the isolated application of salicylic acid and methionine were more intense in BRS Novaera, stimulating the tolerance mechanism of this cultivar to water stress.

Spatial and temporal variations of gross primary production simulated by land surface model BCC_AVIM2.0

Gross primary production (GPP) is the largest flux and a crucial player in the terrestrial carbon cycle and has been studied extensively, yet large uncertainties remain in the spatiotemporal patterns of GPP in both observations and simulations. This study evaluates the performance of the second version of the Beijing Climate Center Atmosphere−Vegetation Interaction Model (BCC_AVIM2.0) in simulating GPP on multiple spatial and temporal scales in the Coupled Model Intercomparison Project Phase 6 (CMIP6) experiments. Model simulations driven by two meteorological datasets were compared with two observation-based GPP products covering 1982–2008. Spatial patterns of annual GPP show a significant latitudinal gradient in each dataset, increasing from cold (tundra) and dry (desert) biomes to warm (temperate) and humid (tropical rainforest) biomes. BCC_AVIM2.0 overestimates GPP in most parts of the globe, especially in boreal forest regions and Southeast China, while underestimating GPP in subhumid regions in eastern South America and tropical Africa. The four datasets broadly agree on the GPP seasonal cycle, but BCC_AVIM2.0 predicts an earlier beginning of spring growth and a larger amplitude of seasonal variations than those in the observations. The observation-based datasets exhibit slight interannual variability (IAV) and weak GPP linear trends, while the BCC_AVIM2.0 simulations demonstrate relatively large year-to-year variability and significant trends in the low-latitudes and temperate monsoon regions in North America and East Asia. Regarding the possible relationships between annual means of GPP and climate factors, BCC_AVIM2.0 predicts more extensive regions of the globe where the IAV of annual GPP is dominated by precipitation, especially in mid-to-high latitudes of the Northern Hemisphere and tropical Africa, while the observed GPP in the above regions is temperature- or radiation-dominant. The positive GPP biases due to earlier spring growth in boreal forest regions and negative GPP biases in off-equator tropical areas in the BCC_AVIM2.0 simulations imply that cold stress on biomes in boreal mid-to-high latitudes should be strengthened to restrain plant growth, while drought stress in low-latitude regions might be eased to enhance plant production in the future version of BCC_AVIM.

Regional brain glucose metabolism is differentially affected by ketogenic diet: a human semiquantitative positron emission tomography.

Ketogenic diet (KD) is recommended to avoid intense [18F]FDG myocardial physiologic uptake in PET imaging. Neuroprotective and anti-seizure effects of KD have been suggested, but their mechanisms remain to be elucidated. This [18F]FDG PET study aims to evaluate the effect of KD on glucose brain metabolism. Subjects who underwent KD prior to whole-body and brain [18F]FDG PET between January 2019 and December 2020 in our department for suspected endocarditis were retrospectively included. Myocardial glucose suppression (MGS) on whole-body PET was analyzed. Patients with brain abnormalities were excluded. Thirty-four subjects with MGS (mean age: 61.8 ± 17.2years) were included in the KD population, and 14 subjects without MGS were considered for a partial KD group (mean age: 62.3 ± 15.1years). Brain SUVmax was first compared between these two KD groups to determine possible global uptake difference. Semiquantitative voxel-based intergroup analyses were secondarily performed to determine possible inter-regional differences by comparing KD groups with and without MGS, separately, to 27 healthy subjects fasting for at least 6h (mean age of 62.4 ± 10.9years), and KD groups between them (p-voxel < 0.001, and p-cluster < 0.05, FWE-corrected). A 20% lower brain SUVmax was found in subjects under KD with MGS in comparison to those without MGS (Student's t-test, p = 0.02). Whole-brain voxel-based intergroup analysis revealed that patients under KD with and without MGS had relative hypermetabolism of limbic regions including medial temporal cortices and cerebellum lobes and relative hypometabolism of bilateral posterior regions (occipital), without significant difference between them. KD globally reduces brain glucose metabolism but with regional differences, requiring special attention to clinical interpretation. On a pathophysiological perspective, these findings could help understand underlying neurological effects of KD through possible decrease of oxidative stress in posterior regions and functional compensation in the limbic regions.

Biomechanical Analysis of Femoral Stem Features in Hinged Revision TKA with Valgus or Varus Deformity: A Comparative Finite Elements Study

Hinged total knee arthroplasty (TKA) is a valid option to treat patients during revision of an implant; however, in case of varus/valgus deformity, the force transmission from the femur to the tibia could be altered and therefore the performance of the implant could be detrimental. To be able to evaluate this, the goal of this study was to investigate, using a validated finite element analysis, the effect of varus/valgus load configurations in the bones when a hinged TKA is used. In detail, short and long stem lengths (50 mm, and 120 mm), were analyzed both under cemented or press-fit fixation under the following varus and valgus deformity: 5°, 10°, 20°, and 30°. The main outputs of the study were average bone stress in different regions of interest, together with tibio-femoral contact pressure and force. Results demonstrated that changes in the varus or valgus deformity degrees induce a change in the medio-lateral stress and force distribution, together with a change in the contact area. The effect of stem length and cement do not alter the tibio-femoral contact biomechanics but its effect is mainly localized in the distal femoral region, and it is negligible in the proximal regions.

Monitoring of Drought Stress in Chinese Forests Based on Satellite Solar-Induced Chlorophyll Fluorescence and Multi-Source Remote Sensing Indices

Greenhouse gas emissions have largely changed the global climate, leading to an increase in the frequency and extent of droughts. Forests are essential natural resources, and they play an important role in maintaining ecological security. Effectively monitoring drought stress in forests can help promote sustainable forestry development. Solar-induced chlorophyll fluorescence is a spectral signal released by vegetation photosynthesis after light absorption. In this study, we used solar-induced chlorophyll fluorescence data (SIF), canopy fluorescence yield (SIFyield) data, vegetation indices (NDVI, EVI), leaf area index (LAI), and fraction of absorbed photosynthetically active radiation (fPAR) to study forest drought stress in the Yunnan, Fujian, Shaanxi, and Heilongjiang provinces in China, respectively. The temporal and spatial ranges of drought stress indicated by the Standardized Precipitation-Evapotranspiration Index (SPEI) values were used as a reference (SPEI ≤ −0.5 indicates the occurrence of drought). Firstly, the standardized anomalous values of SIF, SIFyield, NDVI, EVI, LAI, and fPAR were calculated. The temporal and spatial response abilities of each variable to drought stress were analyzed. Secondly, the correlation between each variable and the drought indicator SPEI was quantified. Finally, the validity and variability of SIF and other variables for drought monitoring were analyzed and verified with a random forest classification model. The results showed that on a temporal scale, SIFyield showed an earlier response to drought stress than other variables and the abnormal change of SIFyield was higher than other variables by 10% or more. Spatially, the range of drought areas indicated by SIFyield and SPEI had more coincident areas than other variables. The overall correlation between SIFyield and SPEI was also higher during the drought period, especially during late drought periods when other variables showed negative correlations. For SIFyield, the correlation coefficients of the Yunnan, Fujian, Shaanxi, and Heilongjiang provinces were 0.57, 0.43, 0.32, and 0.49, respectively. Additionally, the variable importance assessment using a random forest model also indicated that SIFyield is more sensitive to forest droughts. We concluded that SIFyield is an effective tool for monitoring forest drought stress in various regions of China and that it can provide a scientific basis for forest drought monitoring.

Neutral polymers partial exclusion in ionic solutions near charged membranes.

Neutral polymers have become a useful tool in biophysics. They are currently used in a variety of single-channel conductance measurements, to change solution conductivity and viscosity, to induce osmotic stress in polymer-excluded regions, or simply as crowding agents. Many experiments involve the proximity of neutral polymers to charged membranes or their confinement in aqueous pores formed by protein channels with charged residues. A well-known phenomenon relevant to this problem is the polarization of a neutral particle with dielectric properties different from the surrounding medium under the effect of an electric field. This polarization causes a dielectrophoretic force when inhomogeneous electric fields act on the particle and it has been extensively used in many biomedical applications involving particle trapping and cell separation, among others. In addition, whenever a neutral particle perturbs the equilibrium electric double layer near a charged surface, the osmotic pressure gradient acts as a net repulsive force that pushes the particle away from that surface. Both the dielectrophoretic force and the hydrostatic force contribute to exclude a neutral particle from a charged surface, provided the polarizability is lower in the particle than in the solution. Here we report analytical predictions and simulations of the exclusion of polyethylene glycol (PEG) molecules of different molecular weight from charged surfaces. Molecular dynamics of PEG near charged DPPS membranes and neutral DPPC membranes show a region of PEG exclusion near the membrane-solution interface for charged membranes. The simulations also point to a preferential alignment of PEG molecules near the charged membrane. The simulations are confirmed by preliminary neutron reflectometry data, which are consistent with an exclusion region of concentrated PEG solutions near the highly charged silicon dioxide/water interface.

Porta-enxerto de cajueiro anão tolerante à salinidade tem melhor homeostase iônica e desempenho morfofisiológico de mudas

ABSTRACT Considering the cashew tree’s relevance and the limitations imposed by salinity stress in semi-arid regions, the use of alternatives capable of mitigating the harmful effects due to salinity is of great importance to the production sector. The use of grafted plants, especially with rootstock made of tolerant materials, influences the accumulation of toxic ions in leaves of grafted seedlings. Thus, the objective of this work was to evaluate morphophysiological characteristics and leaf concentrations of Na+, K+ and Ca+2 of combinations of scion and rootstock of early dwarf cashew, contrasting in terms of salinity tolerance. The experiment was carried out in a completely randomized design with five replicates, in a 4 × 3 factorial arrangement, corresponding to four dwarf cashew scion/rootstock combinations (self-graft CCP 09, CCP 09/CCP 76, self-graft CCP 76, and CCP 76/CCP 09) and three NaCl concentrations (0, 50, and 100 mM L-1). Height, number of leaves, leaf area, dry matter, tolerance index and leaf concentrations of Na+, K+ and Ca+2 were evaluated after 30 days of application of NaCl concentrations. The scion/rootstock combination CCP 76/09 showed tolerance to 50 mM L-1, due to the increase of leaf area and number of leaves. The scion/rootstock combination CCP 76/09 was more suitable, as it kept the leaf K+ concentration and had the lowest Na+ concentration.

Redox Imbalance as a Common Pathogenic Factor Linking Hearing Loss and Cognitive Decline

Experimental and clinical data suggest a tight link between hearing and cognitive functions under both physiological and pathological conditions. Indeed, hearing perception requires high-level cognitive processes, and its alterations have been considered a risk factor for cognitive decline. Thus, identifying common pathogenic determinants of hearing loss and neurodegenerative disease is challenging. Here, we focused on redox status imbalance as a possible common pathological mechanism linking hearing and cognitive dysfunctions. Oxidative stress plays a critical role in cochlear damage occurring during aging, as well as in that induced by exogenous factors, including noise. At the same time, increased oxidative stress in medio-temporal brain regions, including the hippocampus, is a hallmark of neurodegenerative disorders like Alzheimer's disease. As such, antioxidant therapy seems to be a promising approach to prevent and/or counteract both sensory and cognitive neurodegeneration. Here, we review experimental evidence suggesting that redox imbalance is a key pathogenetic factor underlying the association between sensorineural hearing loss and neurodegenerative diseases. A greater understanding of the pathophysiological mechanisms shared by these two diseased conditions will hopefully provide relevant information to develop innovative and effective therapeutic strategies.

Attenuation of negative effects caused by a combination of heat and cadmium stress in Arabidopsis thaliana deficient in jasmonic acid synthesis

Due to recent global warming, heat stress can simultaneously occur with cadmium (Cd) stress in regions suffering from metal pollution. In this study, we investigated the effects of heat, Cd and their combination on the growth and physiological characteristics of Arabidopsis thaliana. Arabidopsis plants were more susceptible to a combination of heat and Cd stress than to each stress applied individually, although the accumulation of Cd in shoots was comparable between plants subjected to Cd stress and the combined stress. Plants subjected to this stress combination showed a dramatic reduction in the accumulation of the photosynthetic reaction center proteins in photosystem II as well as a tendency toward enhanced lipid peroxidation, suggesting that the negative effects of a combination of heat and Cd stresses might be caused by oxidative damage accompanied by damage to the photosynthetic apparatus. Interestingly, aos and lox3 mutants deficient in jasmonic acid (JA) synthesis showed attenuation of the negative effects caused by a combination of heat and Cd stresses on the growth and maximum quantum efficiency of photosystem II. The roles of JA might be altered when heat stress is combined with Cd stress, despite its significance in the tolerance of plants to Cd stress when individually applied, which has been shown in previous studies.

Importance of the silvipastoral system on the animal health and welfare of dairy cattle

Brazil stands out for having one of the largest cattle herds in the world, being the first place in the commercial herd in the whole planet. Within bovine farming, dairy cattle raising stands out for its importance within the national economy because of the representativeness of small family farms, which develop a large part of the national milk production. However, despite the extensive area available for agricultural production, a large part of Brazil is located in the tropical area of the planet. This region is distinguished by high temperatures and high intensity of incident solar radiation. This condition tends to lead the animals to heat stress, thus leading to a decrease in the production of meat, but mainly milk, especially in properties that make use of european breeds. One of the alternatives to overcome the impact of heat stress in tropical regions is to cross european breeds with zebu dairy breeds, as well as seeking measures to reduce the incidence of solar radiation of these animals through integrated systems, such as silvipastoral systems. Thus, this review aims to show that the silvipastoral system is necessary to improve the animal welfare of dairy cattle.

Biopolymer-Based Hydrogels for Harvesting Water from Humid Air: A Review

Despite the shrinkage of water availability worldwide, the water demand has been climbing due to the rapidly increasing human population and the impacts of numerous hydroclimatic factors. To feed the growing population and meet with the exigent need for freshwater, new water sources must be identified and utilized. In recent years, biopolymer-based hydrogels have been explored as a sustainable and inexpensive solution to capture moisture from humid air for water supply. This review summarizes recent advances in the preparation of atmospheric water harvesting hydrogels that are synthesized based on cellulose, chitosan, and/or other biopolymers, as well as their agricultural applications. The properties and performance of different biopolymer-based hydrogels are discussed and compared. Since agriculture accounts for more than 70% of the water consumption, developing a cost-effective hydrogel-based atmospheric water harvesting/supply system could be a promising solution for relieving the elevating water stress in arid and semi-arid regions.

Assessment of the level and structure of stress in tax revenues of the federal and regional budgets

Relevance. In recent years, the Russian economy has faced the global challenges posed by the COVID-19 pandemic and unprecedented sanctions. Understanding how the tax systems of Russian regions respond to these external shocks is crucial for identifying critical stress sources affecting budgets at various levels. Research objective. This study aims to assess and compare tax revenue stress across the federal districts of the Russian Federation, considering different levels of the budget system, and to identify the underlying sources of this stress. Data and methods. The study relies on official data from the Federal Tax Service of the Russian Federation on tax revenues to federal and regional budgets in the federal districts. The analysis covers monthly data from January 2013 to December 2022. The stress index was calculated by measuring the difference between the moving standard deviation and the mean of the annual tax revenue growth rate, with a lag of 1 month. The methodology for decomposing the sources of tax revenue stress was also tested. Results. Over-time assessments of stress indices for tax revenues to federal and regional budgets were obtained for the Russian Federation and its federal districts. The varying levels, dynamics, and budgetary distribution of tax revenue stress across federal districts are explained by differences in the structure, growth rates, and volatility of various taxes, as well as their correlations in these districts. Decomposition of federal and regional tax stress showed the unique role of mineral extraction tax and profit tax as stress amplifiers during crises, while in stable periods, they contribute significantly to stress reduction. The study also establishes the distinct roles of federal districts in intensifying or alleviating overall tax revenue stress during pandemic and sanctions shocks, as well as periods of relative stability. Conclusions. This research demonstrates the importance of assessing and identifying sources of tax revenue stress in regions. Such insights help identify vulnerabilities and reserves for enhancing the resilience of regional and federal budgets through the diversification of regional economic systems and adjustments to tax system rules in response to changing external conditions.

Flash drought drives rapid vegetation stress in arid regions in Europe

Flash droughts are characterised by rapid onset, combined with the potential to severely impact agriculture and ecosystems. However, assessments of the ecological impacts of flash droughts, especially in Europe, are largely lacking. Here we investigate ecosystem responses to flash droughts in Europe between 2001 and 2019 using diverse observational data, including gross primary production (GPP) and leaf area index (LAI). We find that in arid regions an abrupt transition to water-stressed conditions occurs within a few weeks, which negatively affects vegetation status and reduces carbon uptake in the initial stages of drought; normalised anomalies of LAI and GPP decrease to about −0.5. By contrast, vegetation in humid regions is not as severely affected, given that soil moisture recovers relatively quickly. We also show that soil moisture status before the onset of drought significantly impacts the timing (1–5 pentads) and degree (−0.33 to −0.71 of normalised LAI and GPP anomalies) of drought-induced vegetation stress, particularly in arid regions. Our results highlight the regional characteristics of flash drought impacts for more informative monitoring and early warning systems.

Water Resources Evaluation in Arid Areas Based on Agricultural Water Footprint—A Case Study on the Edge of the Taklimakan Desert

Water scarcity is an important factor limiting agricultural development in arid areas. Clarifying and evaluating the current situation of water resources in arid regions is helpful for decision-makers in the rational use of water resources. This study takes a typical arid region located at the edge of Taklamakan Desert-Hotan region as the study area. The water footprint (WF) of the Hotan region was calculated based on 20 years of data information from 2000–2019. An evaluation system was established using four aspects of the WF: structural indicators, efficiency indicators, ecological safety indicators, and sustainability indicators. The results show that the WF of the study area is mainly dominated by blue water consumption, with a proportion of 65.74%. The WF of crop production is larger than that of livestock production. The produced WF of grain crops is the highest of all products with a share of 44.21%. The increase in the local agricultural WF reached 53.18% from 2000 to 2019, but it was still lower than the amount of water available for agriculture. The evaluation results indicated that the region’s WF import dependency is lower than the global level, with an annual average self-sufficiency rate of 91.13% and an increase of 878.95% in the WF economic efficiency index. The agricultural WF produced in Hotan is exported in the form of trade, but the quantitative contribution is small and does little to relieve water stress in other regions. The agricultural water consumption was still within the range of local water resources that could be carried but only 6 years of sustainable water use, and the future development was not optimistic. With the ratio of produced WF to available water resources maintained at about 58%, the local available water resources should be above 43.21 × 108 m3 to initially ensure the sustainable use of water resources. There were 12 drought years in the study period, which are prone to droughts and high disaster levels. The drought-water scarcity systems behaved in three phases: 2000–2011 (uncoordinated level), 2012–2015 (transitional phase), and 2016–2019 (coordinated level). Water scarcity threatened by drought reduced. The occurrence of meteorological droughts was more related to natural factors while the changes in WF were mainly driven by socio-economic elements such as human activities.

Physiological Influence of Water Stress Conditions on Novel HLB-Tolerant Citrus Rootstocks

Citrus are one of the most relevant fruit crops in the Mediterranean basin, which is the second-highest citrus-growing region in the world. However, these crops are mainly grown under Mediterranean semi-arid conditions, characterized by long periods of drought and torrential rain. In this work, we have assessed the response of three promising HLB-tolerant citrus rootstocks (Orange-14, UFR-1, and B11R3T27) to the application of four water stress conditions (Control, Mild water stress, Drought, and Flooding), comparing them with Carrizo citrange. Aerial plant symptoms were recorded during the experimental period, whereas plant water parameters, including stomatal conductance, leaf water potential, and relative water content, were obtained at the end of the assay. For all assessed rootstocks and variables, drought treatment was the most limiting factor, with Carrizo citrange being the most suitable rootstock under this condition. Flooding was the second restrictive treatment, in which UFR-1 was the least affected rootstock. Mid-water stress with 50% water requirements did not differ from the Control treatment, which can help save water resources in semi-arid regions. This information can be helpful for the citrus industry to increase the efficiency of citrus crops subject to water stress in semi-arid regions.