A top-chord-free Vierendeel-truss composite slab (TVCS) comprises a concrete slab, several vertical webs, and a steel bottom chord. In this study, static tests and finite element analyses were conducted based on an actual project to investigate the deformation, crack characteristics, ultimate bearing capacity, and failure mode of the composite slab. The findings indicated that the loading process of this type of floor can be divided into elastic, elastoplastic, and failure stages. The section stress distribution in the elastic stage was further analyzed. The crack development pattern exhibited a fine density in the pure bending sections of the concrete slabs, while demonstrating good overall flexural bearing capacity and ductility for the composite slab. Experimental results were compared with the ANSYS finite element model simulation to validate the accuracy of the simulation. Parametric analysis was conducted to assess the impact of concrete strength, steel strength, vertical web width, and distance ratios on the mechanical characteristics of the composite slab. By introducing an adjustment coefficient for the vertical web distance ratio, a revised calculation formula for flexural bearing capacity was proposed, which aligned well with the finite element analysis.