Nuclear medicine (NM) started in Qatar in the mid-1980s with a 1-head γ-camera in Hamad General Hospital. However, Qatar is expanding, and now Hamad Medical Corp. has 2 NM departments and 1 PET/CT Center for Diagnosis and Research, with several hybrid SPECT/CT and PET/CT cameras. Furthermore, 2 new NM departments will be established in Qatar in the coming 3 y. Therefore, there is a need to optimize radiation protection in NM imaging and establish diagnostic reference levels (DRLs) for the first time in Qatar. This need is not only for the NM part of the examination but also for the CT part, especially in hybrid SPECT/CT and PET/CT. Methods: Data for adult patients were collected from the 3 SPECT/CT machines in the 2 NM facilities and from the 2 PET/CT machines in the PET/CT center. The 75th percentile values (also known as the third quartile) were considered preliminary DRLs and were consistent with the most commonly administered activities. The results for various general NM protocols were described, especially 99mTc-based radiopharmaceuticals and PET/CT protocols including mainly oncologic applications. Results: The first DRLs for NM imaging in Qatar adults were established. The values agreed with other published DRLs, as was the case, for example, for PET oncology using 18F-FDG, with DRLs of 258, 230, 370, 400, and 461-710 MBq for Qatar, Kuwait, Korea, the United Kingdom, and the United States, respectively. Similarly, for cardiac stress or rest myocardial perfusion imaging using 99mTc-methoxyisobutylisonitrile, the DRLs were 926, 976, 1,110, 800, and 945-1,402 MBq for Qatar, Kuwait, Korea, the United Kingdom, and the United States, respectively. Conclusion: The optimization of administered activity that this study will enable for NM procedures in Qatar will be of great value, especially for new departments that adhere to these DRLs.