Streptozotocin-induced Diabetic Wistar Rats Research Articles

Effect of Different Fractions of Lawsonia inermis Linn on Haematobiochemical Changes, Osmotic Fragility and Lipid Profile in Streptozotocin-induced Diabetic Wistar Rats

Effect of Different Fractions of Lawsonia inermis Linn on Haematobiochemical Changes, Osmotic Fragility and Lipid Profile in Streptozotocin-induced Diabetic Wistar Rats

Effect of Modified Diets Pancreatic Oxidative Markers in Streptozotocin-Induced Diabetic Wistar rats Following the Administration of Alpha Lipoic Acid

Despite the availability of various anti-diabetic drugs, diabetes mellitus (DM) remains one of the world’s most prevalent chronic diseases leading to the generation of reactive oxygen species (ROS), triggering oxidative stress as well as numerous cellular and molecular modifications such as mitochondrial dysfunction affecting normal physiological functions in the body. This study aimed to investigate the ameliorative effect of modified diets on pancreatic oxidative markers in streptozotocin-induced diabetic Wistar rats following the administration of Alpha lipoic acid, using standard appropriate techniques. Result established that High protein diet (HPD), and with combined administration with alpha lipoic acid in this research resulted in an increased pancreatic superoxide dismutase (SOD) and catalase (CAT) and glutathione (GSH) levels respectively while decreasing pancreatic malondialdehyde (MDA) levels, hence is affirmed as a beneficial strategy in the management of oxidative stress in diabetes. The present study concludes that high protein diets alone and in combination with ALA decreases pancreatic oxidative stress damage caused by streptozotocin toxicity, hence, has the potential to manage diabetes by reducing Reactice oxygen species (ROS) mediated oxidative stress.

Antidiabetic Effects of Coffee Silverskin Extract in Streptozotocin-Induced Diabetic Wistar Rats

Antidiabetic Effects of Coffee Silverskin Extract in Streptozotocin-Induced Diabetic Wistar Rats

Phytochemical Profiling (GC-MS) of the Leaf Extract of Mangifera indica and its Hypolipidemic Effect on Serum Lipid Profile in Streptozotocin-Induced Diabetic Wistar Rats

Phytochemical Profiling (GC-MS) of the Leaf Extract of Mangifera indica and its Hypolipidemic Effect on Serum Lipid Profile in Streptozotocin-Induced Diabetic Wistar Rats

Diet Supplementation with Rosemary (Rosmarinus officinalis L.) Leaf Powder Exhibits an Antidiabetic Property in Streptozotocin-Induced Diabetic Male Wistar Rats

Diabetes mellitus is a metabolic disorder that has a high global health burden and causes high mortality and morbidity in humans. Medicinal herbs and plants offer a promising alternative to conventional therapies for the management of diabetes. Rosemary (Rosmarinus officinalis L.) is a traditional medicinal herb that has been used for the management of several diseases. Therefore, the present study investigates the antidiabetic properties of diets supplemented with R. officinalis leaf powder on streptozotocin-induced diabetic Wistar rats. First, the phytochemicals and 2,2-dephenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity of aqueous R. officinalis leaf extract were determined. Streptozotocin-induced diabetic male Wistar rats were fed a diet supplemented with R. officinalis leaf powder (ROP) at 3%, 6%, and 12%, respectively, for 6 weeks. Investigations of food intake, body weight, rat relative organ weights, blood glucose, lipid profiles, creatinine, bilirubin, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were estimated according to standard procedures. The results show that ROP aqueous extract contains significant amounts of phenolics, flavonoids, and tannins, which exhibit in vitro DPPH free-radical scavenging activity. Based on an in vivo study, ROP reduced blood glucose levels in streptozotocin-induced diabetic animals (p < 0.05). Dietary supplementation with ROP in diabetic rats significantly (p < 0.05) lowered ALT, AST, bilirubin, creatinine, total triglyceride (TG), total cholesterol (TC), and low-density lipoprotein (LDL) levels while increasing high-density lipoproteins (HDLs) when compared with the diabetic control group. Our findings demonstrate that a diet supplemented with R. officinalis leaf powder exhibits an antidiabetic potential with improved health outcomes, as demonstrated by the improved lipid and liver profile enzymes in our animal model.

Lawsonia inermis Linn. Positively Improves the Expression of Various Inflammatory Cytokines and Apoptotic Cell Death Biomarkers of StreptozotocinInduced Diabetic Wistar Rats

Lawsonia inermis Linn. Positively Improves the Expression of Various Inflammatory Cytokines and Apoptotic Cell Death Biomarkers of StreptozotocinInduced Diabetic Wistar Rats

Effect of selective serotonin reuptake inhibitors on blood glucose in euglycemic and streptozotocin-induced diabetic albino Wistar rats

Effect of selective serotonin reuptake inhibitors on blood glucose in euglycemic and streptozotocin-induced diabetic albino Wistar rats

Pancreatic beta cell regenerative potential of Zanthoxylum chalybeum Engl. Aqueous stem bark extract

Ethnopharmacological relevanceZanthoxylum chalybeum Engl. is endemic to Africa and has been used traditionally to treat diabetes mellitus. Moreover, its pharmacological efficacy has been confirmed experimentally using in vitro and in vivo models of diabetes. However, the effects of Z. chalybeum extracts and its major constituent compounds on beta cell and islet regeneration are not clear. Further, the mechanisms associated with observed antidiabetic effects at the beta cell level are not fully elucidated. Aim of the studyWe determined the beta cell regenerative efficacy of Z. chalybeum aqueous stem bark extract, identified the chemical compounds in Z. chalybeum aqueous stem bark extracts and explored their putative mechanisms of action. Materials and methodsPhytochemical profiling of the Z. chalybeum extract was achieved using ultra high-performance liquid chromatography hyphenated to high-resolution mass spectrometry. Thereafter, molecular interactions of the compounds with beta cell regeneration targets were evaluated via molecular docking. In vitro, effects of the extract on cell viability, proliferation, apoptosis and oxidative stress were investigated in RIN-5F beta cells exposed to palmitate or streptozotocin. In vivo, pancreas tissue sections from streptozotocin-induced diabetic male Wistar rats treated with Z. chalybeum extract were stained for insulin, glucagon, pancreatic duodenal homeobox protein 1 (Pdx-1) and Ki-67. ResultsBased on ligand target and molecular docking interactions diosmin was identified as a dual specificity tyrosine-phosphorylation-regulated kinase 1A (Dyrk1A) inhibitor. In vitro, Z. chalybeum augmented cell viability and cell proliferation while in palmitate-pre-treated cells, the extract significantly increased cell activity after 72 h. In vivo, although morphometric analysis showed decreased islet and beta cell size and density, observation of increased Pdx-1 and Ki-67 immunoreactivity in extract-treated islets suggests that Z. chalybeum extract has mild beta cell regenerative potential mediated by increased cell proliferation. ConclusionsOverall, the mitogenic effects observed in vitro, were not robust enough to elicit sufficient recovery of functional beta cell mass in our in vivo model, in the context of a sustained diabetic milieu. However, the identification of diosmin as a potential Dyrk1A inhibitor merits further inquiry into the attendant molecular interactions.

Volatiles, phenolic compounds and bioactive properties of essential oil and aqueous extract of purple basil (Ocimum basilicum L.) and antidiabetic activity in streptozotocin-induced diabetic Wistar rats

Diabetes represents a significant global health concern, and researchers are actively exploring innovative approaches to managing this disease. In this study, we investigated the bioactive properties and anti-diabetic activity of the aqueous extract (BAE) and essential oil (BEO) derived from purple basil (Ocimum basilicum L.). The antioxidant activity of BAE was found to be higher than that of the essential oil extract from purple basil. Among the phenolic compounds identified, t-caffeic acid was the most abundant, followed by caftaric acid, kaempferol-3-glucoside, and quercetin-3-glucoside. In total, 49 volatile constituents were identified in cold- and hot-brewed basil, with methyl cinnamate, linalool, and eugenol were the major compounds. Both BAE and BEO showed inhibitory effect on the α-glucosidase and α-amylase enzymes. Furthermore, the administration of BAE led to a 37% reduction in blood glucose levels at day 14 and a 25.5% reduction at day 28. Similarly, BEO exhibited a 26.3% decrease in fasting blood glucose levels on the 28th day in rats. These findings highlight the potential of BAE and BEO in reducing blood glucose levels and their consequent antidiabetic activity. Based on the results obtained, we suggest that basil can be utilized in the formulation of functional and dietary foods within the food and beverage industry, owing to its demonstrated antidiabetic properties.

Toll-Like receptors-2 and-4 levels in streptozotocin-induced diabetic Wistar rats

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Evaluation of Antidiabetic and Antioxidant Activities of Fruit Pulp Extracts of Cucurbita moschata Duchesne and Cucurbita maxima Duchesne.

To evaluate and compare the antidiabetic and antioxidant activities of fruit pulp extracts from Cucurbita moschata (PCMOS) and Cucurbita maxima (PCMAX). The antidiabetic activity was carried out in vivo by orally and daily giving the extracts at a dose of 500 mg/kg·b.w. to the streptozotocin-induced diabetic male albino Wistar rats for six weeks. After the period of administration, blood glucose levels, body weight, serum insulin, morphology of islets of Langerhans, biochemical parameters, and haematological values of the rats were determined. Meanwhile, the antioxidant activity was carried out in vitro by determination of total phenolic and flavonoid contents, DPPH radical scavenging activity, and ferric reducing antioxidant power. PCMAX significantly (p < 0.05) reduced blood glucose levels but increased the body weight, serum insulin levels, size and number of islets of Langerhans, and β-cell number of the treated diabetic rats more than PCMOS did. However, they did not alter biochemical parameters and haematological values of the treated diabetic rats. PCMAX possessed total phenolic and flavonoid contents and showed DPPH scavenging and FRAP reducing antioxidant power more significantly (p < 0.05) than PCMOS. According to the obtained results, it is indicated that PCMOS and PCMAX possess antidiabetic and antioxidant activities. PCMAX possesses more potent antidiabetic and antioxidant activities than PCMOS. These are probably due to PCMAX providing polysaccharide and total phenolic and flavonoid contents more than PCMOS.

In vivo antidiabetic and antioxidant activities of Prosopis africana fruit extracts in streptozotocin-induced diabetic Wistar rats

In vivo antidiabetic and antioxidant activities of Prosopis africana fruit extracts in streptozotocin-induced diabetic Wistar rats

Biochemical and histopathological evaluation of ChAdOx1-S and BBIBP-CorV vaccination in normal and streptozotocin-induced diabetic Wistar rats.

Infection with Covid-19 has been associated with some medical complications namely diabetes, thrombosis, and hepatic and renal dysfunction among others. This situation has created some concern about the use of relevant vaccines which might cause similar complications. In that regard, we planned to evaluate the impact of two of the relevant vaccines namely ChAdOx1-S and BBIBP-CorV on some of the blood biochemical factors and also on liver and kidneys functions following the immunization of healthy and streptozotocin-induced diabetic rats. Evaluation of the level of neutralizing antibody among the rats indicated that immunization with ChAdOx1-S induced a higher level of neutralizing antibody among both the healthy and diabetic rats compared to the BBIBP-CorV vaccine. Furthermore, the neutralizing antibody levels against both types of vaccines were significantly lower in diabetic rats than in healthy ones. On the other hand, no alterations were observed in the rats' sera biochemical factors, coagulation values and histopathological images of the liver and kidneys. Altogether these data besides of confirming the effectiveness of both vaccines, indicate that both vaccines have no hazardous side effects on rats and probably humans though clinical investigations are required to validate our present data.

Comparative antihyperglycemic potentials of different fractions of Detarium senegalense stem bark extract on streptozotocin-induced diabetic Wistar rats

Comparative antihyperglycemic potentials of different fractions of Detarium senegalense stem bark extract on streptozotocin-induced diabetic Wistar rats

Comparative antihyperglycemic potentials of different fractions of Detarium senegalense stem bark extract on streptozotocin-induced diabetic Wistar rats

Aim: The search for cheap and potent antidiabetic agent with less adverse effects and efficient glycemic control than orthodox and allopathic therapies prompted this current study. Method: Forty-two (42) adult Wistar rats of between 150-200g were procured and acclimatized for two weeks for the study. They were first grouped into six (6) of seven rats (n=7) per group. Whilst group 1 received normal water and chow ad libitum (control), groups 2-6 respectively were steptozotocin (STZ)-induced diabetic, untreated (diabetic control), 50mg/kg metformin treated diabetic, STZ-induced, 250mg/kg body weight of D. senegalensis ethyl acetate extract treated, STZ-induced, 250mg/kg of D. senegalensis n-hexene extract treated, and 250mg/kg bw of D. senegalensis chloroform extract treated. While using the GCMS to analyze the active ingredients of the various D. senegalensis fractionates administered, fasting blood glucose levels were checked and compared weekly for 10 weeks before and during treatments; obtaining differences in mean with the one-way analysis of variance (ANOVA). All test statistics were performed with the graph pad prism 8.1, setting p &lt; 0.05 as statistically significant. Results: Study observed the highest anti-diabetic bioactivity in experimental than control animals; especially confirming the presence of major phytochemicals but Glycosides. In diabetic rats, a significant increase was observed in α-amylase and α-glucosidase activities, but with administration of chloroform, Hexane and Ethyl acetate fractions from the methanol stem bark extract at 250 mg/kg and metformin (50 mg/kg) significantly decreasing the activity of the enzyme’s ability on the pancreas, with the most potent being the ethyl acetate extract group Conclusion: D. senegalense extract proved to be a potent alpha-glucosidase inhibitor with less potency for alpha-amylase. This action could be productive in preventing DM via decrease in blood glucose uptake.

Influence of Sterculia setigera Delile extract on oxidative stress enzyme activity and treatment of impaired glucose homeostasis in Streptozotocin-induced diabetic Wistar rats

BackgroundSterculia setigera Delile is a plant member of Sterculiacea family whose ethnobotanical investigation has revealed medicinal virtues. Traditional Chinese medicine (TCM) has several advantages as it includes a wide variety of plants, low cost, minimal toxic and side effects. More significantly, many bioactive compounds such as flavonoids, tannins and saponins are active constituents of traditional Chinese medicinal plants, which generally have no adverse effects. This study aimed to evaluate the influence of S. setigera Delile extract in impairing hyperglycemia and oxidative stress induced by streptozotocin in Wistar rats. MethodsThe intraperitoneal injection of streptozotocin (65 mg/kg) generated hyperglycemia in male Wistar rats. Diabetic animals were treated for 4 weeks with S. setigera extract. Insulin concentration and lipid profile biomarkers were evaluated. Oxidative stress biomarkers were assessed in pancreas and kidney. Histological sections of pancreas and kidney were made. ResultsAfter 4 weeks of treatment, the extract could decrease blood glucose levels by CI% (-92.3 to -39.94) compared to the diabetic control. Serum insulin, triglycerides and total cholesterol were improved towards their normal values. Pancreatic and renal cell architecture, were also improved in animals treated with the extract. ConclusionS. setigera extract could thus reverse hyperglycemia, dyslipidemia and oxidative stress in streptozotocin diabetic rats.

Ameliorative Potentials of Methanol Bark Extract of Detarium senegalense (Tallow Tree) in Streptozotocin Induced Diabetic Male Wistar Rats

Diabetes is a global metabolic disease that affects millions of individuals worldwide and raises the global death rate, posing a public health concern. Because antidiabetic traditional drugs are expensive, have side effects, and are ineffective, researchers are looking into novel medicinal plants to find a long-term cure that is inexpensive, has few or no side effects, and is highly effective in treating diabetes and its consequences. Detarium senegalense is a versatile plant used to cure a variety of ailments, including diabetes. This research aimed to investigate the ameliorative potentials of methanol bark extract of Detarium senegalense in streptozotocin-Induced Diabetic male Wistar Rats. Thirty (30) adult male Wistar rats weighing 120-180gm were chosen at random and put into six (6) groups of five rats each (n=5) for this investigation. A single intraperitoneal injection of streptozotocin (150mg/kg) produced diabetes in rats. Normal and diabetic control groups are represented by groups 1 and 2, respectively. Group 3, a diabetic group, received metformin (50mg/kg). Groups 4, 5, and 6 received methanol bark extract of Deterium senegalenses at 250, 500, and 1000 mg/kg/day, respectively. The animals were euthanized at the end of the 21-day treatment period, and organs were excised for biochemical examination. The generated data was analyzed with the SPSS software, and the results were expressed as mean SEM. Result confirms Detarium senegalense methanol bark extract as a hypoglycemic agent on the diabetic rats, by decreasing blood glucose level and the activity of metabolic enzymes (α-amylase and α-glucosidase), increase hexokinase, Superoxide dismutase, Catalase, Glut 1 and Glut 2 activities while decreasing the activities of Malondialdehyde. Conclusively, the bark of Detarium senegalense has been shown to have anti-diabetic and anti-oxidative.

Aspartame consumption causes cognitive impairment in streptozotocin-induced diabetic Wistar rats

Aspartame consumption causes cognitive impairment in streptozotocin-induced diabetic Wistar rats

Immunohistochemical Effects of Ethyl Acetate Fraction of Lycopersicum Esculentus on the Kidney in Experimentally Induced Diabetic Wistar Rats

Diabetic nephropathy (DN) as a leading cause of end-stage renal failure which has become source of concern recently. The aim of this study was to investigate the effect of EAFL on the histomorphology and evaluate its effect on inflammatory markers in Streptozotocin- Induced diabetic Wistar rats. This study was conducted using thirty male Wistar rats. The animals were divided into five groups of 6 rats each (n=6). Group A was the control animals ( administered with 20 ml/kg/day of citrate buffer orally (p.o) stat for 4 weeks. Group B was induced with 65 mg/kg of streptozotocin in citrate buffer intraperitoneally stat and observe for four weeks without treatment. Group C,D and E were the test groups that received 65 mg/kg of streptozotocin in citrate buffer intraperitoneally stat and 20, 40 and 60 mg/kg/day of EAFL respectively for four weeks after induction of diabetes. Descriptive and inferential statistics were used to analyze the data. The results showed reduction in serum insulin levels in diabetic groups which was reversed to normal when the intervening agent (EAFL) was administered. The histology of the kidneys showed distortion in renal corpuscles which was reversed when EAFL was administered. The immunohistochemistry of kidney tissues in the normal group (A) showed areas of Ag-Ab immunological reactivity, however, there was no reactivity in the diabetic groups. The study concluded that EAFL has anti glycemic and anti inflammatory effect on the kidneys of Streptozotocininduced diabetic Wistar rats.

Influence of lauric acid on the relaxation of corpus cavernosum in streptozotocin-induced diabetic male Wistar rats

BackgroundDiabetes mellitus is a global health challenge and has been recognised as a risk factor for erectile dysfunction. Dissatisfaction with standard medications has been reported by some patients, hence therapeutic plants are being considered as a viable alternative therapy, with their active components being investigated to create a standard regimen. Lauric acid is the most abundant constituent of coconut oil and is proposed to be responsible for its therapeutic properties. The corpus cavernosum plays an important role in erectile function with its relaxation favouring erection. This study thus sought to investigate the possible relaxant action and mechanism of lauric acid on the corpus cavernosum of diabetic male Wistar rats. Diabetes was induced by intraperitoneal injection of streptozotocin after which graded doses of lauric acid were administered orally to three groups of diabetic rats, once daily for 4 weeks. At the end of the experiment, the corpus cavernosal tissues of the rat penis were extracted. Following phenylephrine or potassium chloride (KCl)—induced contraction, relaxation response to acetylcholine and sodium nitroprusside was used to evaluate endothelium-dependent and nitric oxide-mediated relaxation, respectively.ResultsRelaxation response to acetylcholine, following pre-contraction with phenylephrine, was significantly decreased in the cavernosal tissues of diabetic untreated rats and was not significantly improved in lauric acid treated diabetic groups. Relaxation response to acetylcholine, following pre-contraction with KCl, was significantly decreased in the diabetic untreated group but was significantly improved in lauric acid treated diabetic groups at the lowest dose. Decreased relaxation response to sodium nitroprusside, following pre-contraction with phenylephrine in tissues of diabetic untreated rats, was significantly improved in lauric acid-treated diabetic groups at lower doses. Decreased relaxation response to sodium nitroprusside, following pre-contraction with KCl, was significantly improved in lauric acid-treated diabetic groups at all doses.ConclusionLauric acid improved relaxation of corpus cavernosum muscle in diabetic male rats by enhancing nitric oxide-mediated relaxing action of sodium nitroprusside and possibly inhibiting KCl-induced contraction.