Species Of Streptosporangium Research Articles

Streptosporangium subfuscum sp. nov., isolated from the rhizosphere of marigold (Tagetes erecta L.).

A Gram-stain positive, filamentous bacterial strain, designated strain NEAU-TWSJ13(T), was isolated from the rhizosphere of a marigold (Tagetes erecta L.) plant collected in Heilongjiang Province, northeast China, and characterized using a polyphasic approach. The strain was observed to form abundant aerial hyphae differentiated into spherical sporangia. 16S rRNA gene sequence similarity studies showed that strain NEAU-TWSJ13(T) belongs to the genus Streptosporangium, being most closely related to Streptosporangium fragile DSM 43847(T) (98.6 %). Phylogenetic analysis of the 16S rRNA gene sequence indicated that it formed a phyletic line with S. fragile DSM 43847(T), Streptosporangium jomthongense NBRC 110047(T) (98.4 % 16S rRNA gene similarity) and Streptosporangium violaceochromogenes DSM 43849(T) (97.6 % 16S rRNA gene similarity). A combination of DNA-DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-TWSJ13(T) can be distinguished from S. fragile DSM 43847(T) and S. jomthongense NBRC 110047(T). Moreover, strain NEAU-TWSJ13(T) can also be differentiated from S. violaceochromogenes DSM 43849(T) and other Streptosporangium species showing high 16S rRNA gene sequence similarity (>98.0 %) by morphological and physiological characteristics. Therefore, it is proposed that strain NEAU-TWSJ13(T) represents a novel species of the genus Streptosporangium, for which the name Streptosporangium subfuscum sp. nov. is proposed. The type strain is NEAU-TWSJ13(T) ( = CGMCC 4.7146(T) = DSM = 46724(T)).

Streptosporangium nanhuense sp. nov., a novel actinomycete isolated from soil

A novel actinomycete, designated strain NEAU-NH11(T), was isolated from muddy soil collected from a lake and characterized using a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain NEAU-NH11(T) belongs to the genus Streptosporangium, and was most closely related to Streptosporangium amethystogenes subsp. amethystogenes DSM 43179(T) (99.0%). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-NH11(T) formed a monophyletic clade with Streptosporangium purpuratum CY-15110(T) (98.3%) and Streptosporangium yunnanense CY-11007(T) (98.0%), an association that was supported by a bootstrap value of 80% in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. However, the low level of DNA-DNA relatedness allowed the strain to be differentiated from S. amethystogenes subsp. amethystogenes DSM 43179(T), S. purpuratum CY-15110(T) and S. yunnanense CY-11007(T). Moreover, strain NEAU-NH11(T) could also be differentiated from its closest related strains by phenotypic characteristics. Therefore, it is proposed that strain NEAU-NH11(T) represents a novel Streptosporangium species, Streptosporangium nanhuense sp. nov. The type strain of S. nanhuense is NEAU-NH11(T). (=CGMCC 4.7131(T)=DSM 46674(T)).

Streptosporangium shengliensis sp. nov., a novel actinomycete isolated from a lake sediment

A novel actinomycete, designated strain NEAU-GH7(T), was isolated from a lake sediment and characterized using a polyphasic approach. Strain NEAU-GH7(T) was Gram-stain positive, aerobic, non-spore-forming and produced spherical sporangia. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-GH7(T) formed a monophyletic clade with the closest relative Streptosporangium longisporum DSM 43180(T) (99.0 %), an association that was supported by a bootstrap value of 74 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. However, the low level of DNA-DNA relatedness allowed the strain to be differentiated from its closest relative. Moreover, strain NEAU-GH7(T) could also be differentiated from S. longisporum DSM 43180(T) and other Streptosporangium species showing high 16S rRNA gene sequence similarity (>98.0 %) by morphological and physiological characteristics. On the basis of phylogenetic analysis, DNA-DNA hybridization and phenotypic characteristics, strain NEAU-GH7(T) should be classified as a new species of the genus Streptosporangium, for which the name Streptosporangium shengliense [corrected] sp. nov. is proposed. The type strain is NEAU-GH7(T) (=CGMCC 4.7105(T)=DSM 45881(T)).

Streptosporangium anatoliense sp. nov., isolated from soil in Turkey

A novel actinobacterium, strain N9999(T), was isolated from soil and its taxonomic position determined using a polyphasic approach. The organism formed abundant aerial hyphae that differentiated into spherical spore vesicles. The cell wall contained meso-diaminopimelic acid; the whole-cell sugars were galactose, glucose, mannose, madurose and ribose; the predominant menaquinones MK-9 (H(2)) and MK-9 (H(4)); the major phospholipids phosphatidylethanolamine, diphosphatidylglycerol, a phosphaglycolipid and phosphatidylinositol mannosides; while the cellular fatty acids were rich in iso-C(14:0), C(15:0), cis-9-C(17:1), iso-C(16:0) and 10-methyl C(17:0) components. Phylogenetic analyses based on an almost complete 16S rRNA gene sequence indicated that strain N9999(T) was closely related to a group that consisted of Streptosporangium pseudovulgare DSM 43181(T) and Streptosporangium nondiastaticum DSM 43848(T). However, DNA-DNA relatedness and phenotypic data demonstrated that strain N9999(T) was clearly distinguished from all closely related Streptosporangium species. The combined genotypic and phenotypic data demonstrate conclusively that the isolate should be classified as a new species of Streptosporangium.

Thermotolerant and thermophilic actinomycetes from soils of Mongolia desert steppe zone

In the actinomycete complexes of Mongolian desert soils, thermotolerant and thermophilic actinomycetes were found in high abundance, exceeding that of the mesophilic forms. Among the thermotolerant members of the order Actinomycetales, Streptomyces, Micromonospora, Actinomadura, and Streptosporangium species were most widespread in desert soils. Experiments with soil microcosms demonstrated that thermophilic actinomycetes in desert soils grew, developed, and formed mycelia of the length comparable to that of the mesophilic forms of actinomycetes. Molecular biological investigation of the samples of desert steppe soils by denaturing gradient gel electrophoresis (DGGE) and fluorescent in situ hybridization (FISH) revealed members of the phylum Actinobacteria. FISH analysis revealed that the biomass of the metabolically active mycelial actinobacteria in the prokaryotic community of Mongolian desert soils exceeded that of the unicellular Actinobacteria.

Streptosporangium canum sp. nov., isolated from soil

An actinomycete strain, designated HBUM 170018(T), was isolated from soil from Hebei Province, China, and subjected to a polyphasic taxonomic analysis. This study included morphological and physiological investigations and analyses concerning cell chemistry, genomic DNA G+C content, DNA-DNA hybridization and phylogeny. The data obtained were consistent with the assignment of strain HBUM 170018(T) to the genus Streptosporangium and were confirmed by the results of 16S rRNA gene sequence analysis. Strain HBUM 170018(T) can be differentiated from all Streptosporangium species with validly published names with reference to phenotypic characteristics, phylogenetic data and DNA-DNA hybridization results. On the basis of 16S rRNA gene sequencing, DNA-DNA hybridization and phenotypic data, strain HBUM 170018(T) represents a novel species of the genus Streptosporangium, for which the name Streptosporangium canum sp. nov. is proposed. The type strain is HBUM 170018(T) (=7177(T) =DSM 45034(T) =CGMCC 4.2126(T)).

Isolation and partial characterization of pigment-like antibiotics produced by a new strain of Streptosporangium isolated from an Algerian soil

Identification of a new actinomycete strain Sg3, belonging to the genus Streptosporangium and partial characterization of the produced antibacterial activities. The strain Sg3 was isolated from an Algerian Saharan soil and identified by morphological, chemotaxonomic and phylogenetic analyses to the genus Streptosporangium. The comparison of its physiological characteristics with those of known species of Streptosporangium showed significant differences with the nearest species Streptosporangium carneum. Analysis of the 16S rDNA sequence of strain Sg3 showed a similarity level ranging between 97% and 98.8% within Streptosporangium species, with S. carneum the most closely related. Strain Sg3 showed a red coloured antibacterial activity against gram-positive bacteria on several culture media. The purification of the red pigment by chromatographic methods led to the isolation of three active products. The (1)H nuclear magnetic resonance (NMR), mass, infrared (IR) and ultraviolet-visible (UV-VIS) data of these molecules strongly suggested that they belonged to the quinone-anthracycline group with three or more rings. Strain Sg3 represents a distinct phyletic line suggesting a new genomic species. It produces antibacterial activities identified as quinone-anthracycline aromatics. The quinone-anthracycline antibiotics are known for their antimicrobial and antineoplastic activities and are used in chemotherapy for the treatment of many cancer diseases. The present work constitutes the first stage of a whole series of studies to be realized on these antibiotics before arriving at a possible application.

Taxonomy and chemical characterization of antibiotics of Streptosporangium Sg 10 isolated from a Saharan soil

A new actinomycete strain designated Sg 10, producing antimicrobial substances was isolated from an Algerian soil. Morphological and chemical studies indicated that strain Sg 10 belonged to the genus Streptosporangium. The comparison of its physiological characteristics with those of known species of Streptosporangium showed significant differences with the nearest species Streptosporangium carneum. Analysis of the 16S rDNA sequence of strain Sg 10 showed a similarity level ranging between 96.3% and 97.8% within Streptosporangium species, with S. carneum the most closely related. However, the phylogenetic analysis indicated that strain Sg 10 represent a distinct phyletic line suggesting a new genomic species. The antimicrobial activity of strain Sg 10 showed an antibacterial activity against Gram-positive bacteria as well as an antifungal one. Four active products were isolated from the culture broth using various separation procedures. On the basis of UV-VIS spectrometry, infrared spectroscopy and chemical revelations, the antibiotics were classified in the group of glycosylated aromatics.

Streptosporangium yunnanense sp. nov. and Streptosporangium purpuratum sp. nov., from soil in China

Two strains of Streptosporangium were isolated from Yunnan Province, a region of China with specific geographical conditions that contribute to its great microbiological diversity. They were identified using a polyphasic approach employing phenotypic, genotypic and phylogenetic techniques, such as study of morphological and physiological properties, cell chemistry, G+C content of the genomic DNA, DNA-DNA hybridization and phylogenetic analysis. The strains belong to two novel species of Streptosporangium on the basis of 16S rRNA gene sequencing. The results of morphological, physiological and biochemical investigations and DNA-DNA hybridization indicated that the two strains are different from known members of the genus Streptosporangium. The names Streptosporangium yunnanense sp. nov. (type strain CY-11007(T)=CCTCC AA 97009(T)=CCRC 16307(T)=DSM 44663(T)) and Streptosporangium purpuratum sp. nov. (type strain CY-15110(T)=CCTCC AA 97010(T)=CCRC 16308(T)=DSM 44688(T)) are proposed. They have been deposited in CCTCC in Wuhan.

Streptosporangium subroseum sp. nov., an actinomycete with an unusual phospholipid pattern.

A strain of Streptosporangium with an unusual phospholipid pattern was isolated from Yunnan Province, a region in China. The isolate, designated CY-7113T, was identified by morphological and physiological properties, cell chemistry, genomic DNA G+C content, DNA-DNA hybridization and phylogenetic analysis. Based on 16S rRNA gene sequencing, DNA-DNA hybridization, phenotypic characteristics and its unusual phospholipid pattern, it was concluded that strain CY-7113T belongs to a novel Streptosporangium species, for which the name Streptosporangium subroseum sp. nov. is proposed. Strain CY-7113T (= CCTCC 97008T = CRC 16302T) is the type strain.

Phylogenetic analysis reveals new relationships among members of the genera Microtetraspora and Microbispora.

The 16S rRNA gene sequences of 7 Microbispora strains, 14 Microtetraspora species, 9 Streptosporangium species, and 12 Actinomadura species were determined. A phylogenetic analysis showed that Microtetraspora fusca, Microtetraspora glauca, and Microtetraspora niveoalba formed a coherent cluster with the members of the genus Microbispora. This cluster is distantly related to two other clusters, one of which consists of the Microtetraspora species transferred from the former Actinomadura pusilla group and one of which consists of members of the genus Streptosporangium. Our results show that it is necessary to review the taxonomic definitions of the genera Microtetraspora and Microbispora, which have been separated by a single morphological characteristic, the number of spores on spore chains.

The Phylogenetic Structure of the Genus Streptosporangium

Summary The phylogenetic structure of the genus Streptosporangium was determined by comparative 16S rDNA sequence analysis of 26 Streptosporangium strains, including the type strains of all validly-described Streptosporangium species. Streptosporangium forms a phylogenetically coherent genus and appears to be most closely related to members of the genus Streptomyces . Ten of the thirteen type strains investigated were shown to be highly related, forming strain cluster I, with a substructure of five lineages. This cluster includes the type strain of the type species of the genus, S. roseum DSM 43021 T . Strain cluster II is composed of the other three type strains, i. e., S. corrugatum DSM 43316 T , S. claviforme DSM 44127 T and S. viridialbum DSM 43181 T . Five non-type strains of validly described Streptosporangium species were also investigated; four of these were highly related to their respective type strains, while the other was more closely related to the type strain of another species. Of seven non-validly described taxa studied, four were highly related to S. roseum DSM 43021 T , belonging to strain cluster I, one forms a potential sixth subcluster of cluster I, one belongs to cluster II, and S. “sibiricum” fell outside the radiation of Streptosporangium . Characteristic patterns of signature oligonucleotides differentiate members of Streptosporangium from members of Streptomyces , cluster I organisms from those of cluster II, and the strains of the different subclusters of cluster I from each other. These signatures could be used for future design of specific oligonucleotide probes or primers.

Transfer of Streptosporangium viridogriseum (Okuda et al. 1966), Streptosporangium viridogriseum subsp. kofuense (Nonomura and Ohara 1969), and Streptosporangium albidum (Furumai et al. 1968) to Kutzneria gen. nov. as Kutzneria viridogrisea comb. nov., Kutzneria kofuensis comb. nov., and Kutzneria albida comb. nov., Respectively, and Emendation of the Genus Streptosporangium

A chemotaxonomic analysis of 14 Streptosporangium species revealed the phenotypic heterogeneity of this genus. The type species, Streptosporangium roseum, and 11 other species share similar isoprenoid quinones, fatty acids, and polar lipids. Two species, Streptosporangium albidum and Streptosporangium viridogriseum, including S. viridogriseum subsp. viridogriseum and S. viridogriseum subsp. kofuense, were characterized by a pattern of chemotaxonomic properties that was clearly distinct from the pattern obtained for the authentic Streptosporangium species. The results of a previous phylogenetic analysis of 16S ribosomal DNA and 5S rRNA indicated that the type species and its relatives are distantly related to members of the genus Streptomyces, whereas a member of the other group was found to be phylogenetically related to, but clearly distinct from, members of the family Pseudonocardiaceae. On the basis of these phenotypic and genomic differences, we propose the name Kutzneria gen. nov. for a new genus containing the previously misclassified Streptosporangium species and describe Kutzneria viridogrisea comb. nov., Kutzneria albida comb. nov., and Kutzneria kofuensis comb. nov. The type species of the new genus is Kutzneria viridogrisea, and the type strain is DSM 43850 (=ATCC 25242).

Isolation and characterization of the substance isolated from Streptosporangium species which inhibits lactic acid production by oral bacteria

1. 1. The tentatively named MLI, which inhibits lactic acid production in mutans streptococci, was isolated from Streptosporangium species and purified by solvent extraction followed by chromatography, and then powdered. 2. 2. MLI greatly inhibited pH-decline and lactic acid production in glucose-containing reaction mixture by mutans streptococci or Lactobacillus species. 3. 3. These inhibitory mechanisms were not caused by an anti-bacterial effect, since the addition of a sufficient concentration of MLI to inhibit lactic acid production to the culture, recovery of cells in culture was not affected, excluding L. acidophilus. 4. 4. MLI directly affected lactate dehydrogenase (LDH) activity. 5. 5. These results indicate that MLI may represent a new anti-cariogenic substance.

Physical characterization of plasmids isolated from Streptosporangium

Plasmids were isolated from two species of Streptosporangium by CsCl-ethidium bromide equilibrium density gradient centrifugation. A plasmid isolated from S. brasiliense, designated pSgB-1, was characterized by electron microscopy and agarose gel electrophoresis. The pSgB-1 plasmid is a closed circular DNA molecule of 9.4 × 10 6 Da. A restriction endonuclease map was generated and unique cleavage sites were found for EcoRI, ClaI, XbaI, and MstII. Another plasmid, pSgV-1, isolated from S. viriodogriseum, has an estimated M r of 54 × 10 6. The pSgB-1 plasmid is phenotypically cryptic but an unusual phenotypic trait, resembling phage plaques, may be associated with the S. viridogriseum plasmid pSgV-1.

Streptosporangium fragile sp. nov.

A new species of Streptosporangium is described, for which we propose the name Streptosporangium fragile. This organism produces a new anthracycline antibiotic and is characterized by dark brown to black vegetative mycelium, pink aerial mycelium, brown soluble pigment, and fragile sporangial membrane. In older cultures the fragility of the sporangial membrane results in the coalescence of the sporangiospores into large irregular masses. The type strain of S. fragile is SK&F-BC2496 (= ATCC 31519).

Letter: Antitumor agents. XIII. Isolation and absolute configuration of carminomycin I from Streptosporangium sp.

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAntitumor agents. XIII. Isolation and absolute configuration of carminomycin I from Streptosporangium speciesMansukh C. Wani, Harold L. Taylor, Monroe E. Wall, Andrew T. McPhail, and Kay D. OnanCite this: J. Am. Chem. Soc. 1975, 97, 20, 5955–5956Publication Date (Print):October 1, 1975Publication History Published online1 May 2002Published inissue 1 October 1975https://doi.org/10.1021/ja00853a073RIGHTS & PERMISSIONSArticle Views97Altmetric-Citations32LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (296 KB) Get e-AlertsSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts