To assess the influence of salivary bacterial flora in the complete loss of tooth structure leading to extraction. The objective of this study is to evaluate the influence of salivary levels of Streptococcus mutans and Lactobacillus that leads to tooth extraction. If the etilogy is known prophylactic measures or the steps to retain the tooth can be initiated at the early stage of the lesion. Dental extraction is the process of removal of tooth from the dental socket in the alveolar bone. It is performed for wide variety of reasons the most common reason is tooth that has become unrestorable through tooth decay and the surrounding tissues. This is caused by both facultative anaerobes and obligate anaerobes. This study is aimed to assess the contribution of two major populations of bacterial species in saliva that contributes to tooth loss. The study comprised of 60 subjects divided into three groups, 20 healthy individuals, 20 individuals both male and female reporting for restoration due to dental caries and 20 subjects both male and female reported for tooth removal were randomly selected. The saliva samples were collected in sterile container and subjected for microbiological analysis. The study was conducted in the year 2019. There was a significant increase in the mutans and lactobacillus in the patients coming for dental extraction. This study concludes that there is a many fold increase in the total bacterial count of the saliva and there is also a significant proportionate increase in Streptococcus mutans and Lactobacilli. Further study can be conducted to demonstrate that penetration into deeper layers and bone structures.