Laser assisted suture has become a focus in surgical suture research due to its many advantages, but the current research on the details is not deep enough. To improve the strength and quality of laser biological tissue welding and increase the success rate of penetration in the thickness direction, the effect of external restraint force on the laser tissue welding was explored, and the effect of external restraint force on the optical properties of skin tissue was analyzed. In this study, a Nd:YAG pulse laser was used to conduct welding experiments on the skin in vitro. The laser of 10 W scanned the tissue incision along a linear trajectory. The welding effects under different compression forces and stretching forces were compared. Under the effect of compression force, the skin tissue incision has improved the absorption of laser, and achieved good suture of the incision. The maximum tensile strength can reach 15.56 ± 0.61 N/cm2.The results show that the external preloading force have a significant effect on the strength of the tissue under laser welding. The optical properties of skin tissue under different forces were tested, proving that compression force changes the absorption rate of skin tissue to laser, thereby promoting the photothermal conversion efficiency and improving the welding strength. The stretching force changes the thickness of the tissue, making it easier for the laser to penetrate. At the same time, the tissue is closely attached to achieve effective contact and improve the success rate of welding. The matching combination of preloading force and laser process parameters can achieve high strength with low thermal damage.