Monkeypox virus (MPXV) core cysteine proteinase (CCP) is one of the major drug targets used to examine the inhibitory action of chemical moieties. In this study, anin silico technique was applied to screen 1395 anti-infective compounds to find out the potential molecules against the MPXV-CCP. The top five hits were selected afterscreening and processed for exhaustive docking based on the docked score of ≤ -9.5kcal/mol. Later, the top three hits based on the exhaustive-docking score and interaction profile were selected to perform MD simulations. The overall RMSD suggested that two compounds, SC75741and ammonium glycyrrhizinate, showed a highly stable complex with a standard deviation of 0.18 and 0.23nm, respectively. Later, the MM/GBSA binding free energies of complexes showed significant binding strength with ΔGTOTALfrom -21.59 to -15kcal/mol.This report reported the potential inhibitory activity of SC75741 and ammonium glycyrrhizinate against MPXV-CCP by competitively inhibiting the binding of the native substrate.