Dust Research Articles

Tracking of microplastics distribution patterns and their characterisation in deposited road dust from Dhaka city, Bangladesh

The widespread presence of microplastics (MPs) in road dust has considerable concern regarding their potential risks to ecosystems and human health. Despite the massive production of plastic, the erudition of MPs distribution patterns in various sizes of deposited road dust is still limited around the globe. Thus, the aim of this research is to provide an unambiguous picture of MPs distributional pattern, identification, classification, quantification, and features from road dust in various zones and types of roadways in Dhaka, Bangladesh. This study examined MPs in road dust samples with particle sizes ranging from 300 to 150 μm (Group-A), 149-75 μm (Group-B), and <75 μm (Group-C). This work extracted MPs from road dust using 30 % H2O2, 1.6 g/cm3 ZnCl2, and 0.45 μm filter paper. A fluorescent microscope (Motic B410E, Germany), Motic Pictures, and 3.0 ML software were utilized to identify MPs visually. Additionally, FTIR and SEM were utilized to determine the chemical composition of MPs. Group-A Road dust samples had a significantly higher concentration of MPs (38945 items/kg) compared to Group-B and <75 μm Group-C dust (16720 and 5945 items/kg, respectively). The distribution hierarchy for total MPs on average by location and type of road is as follows: paved road (355 items/5 g) > unpaved road (325 items/5 g) > soil samples (294 items/5 g), roadside dust samples (284 items/5 g), and mid-road (283 items/5 g). By taking into account all sizes of road dust samples, the MPs were classified as fiber (70.26 %), fragment (26.12 %), beads (0.66 %), films (1.32 %), and foams (1.58 %). It was found that adults inhaled MPs an average of 1612 items/day, while they ingested an average of 880 items/day. Children had an inhalation rate of 1232 items/day and an ingestion rate of 10267 items/day, which was 4–17 times greater than in other countries. This study identified 22 MPs polymer types, and SEM results show that MPs surfaces are being weathered into nanoplastics, creating a more hazardous environment.

Quantification of tire wear particles in road dust based on synthetic/natural rubber ratio using pyrolysis-gas chromatography–mass spectrometry across diverse tire types

Increase in road traffic leads to increased concentrations of tire-wear particles (TWPs), a prominent source of microplastics from vehicles, in road dust. These particles can re-enter the atmosphere or move into aquatic ecosystems via runoff, impacting the environment. Consequently, accurately assessing and managing TWP levels in road dust is crucial. However, the ISO method (ISO/TS 20593 and 21396) uses a constant ratio of styrene-butadiene rubber (SBR) to natural rubber (NR) for all tires, disregarding the variability in tire composition across different types and brands. Our study found substantial SBR content (15.7 %) in heavyweight truck tires, traditionally believed to be predominantly NR. We evaluated the SBR/NR content in 15 tire types and proposed a method to more accurately evaluate TWP concentrations in road dust from five different locations. Our findings suggest that the conventional ISO method may underestimate the concentrations of TWP due to its reliance on a static ratio of SBR/NR. This study underscores the necessity for a more flexible approach that can adapt to the variability in SBR and NR content across different tire types. By delineating the limitations inherent in current assessment methods, our research contributes to a more adaptable understanding of TWP concentrations in road dust. This advancement prompts the development of a revised methodology that more accurately reflects the diverse compositions of tire rubber in environmental samples.

Analysis of allergen-specific IgE in children with atopic dermatitis from 2021 to 2023 in a hospital of pediatric in Tianjin City

Objective: To explore the distribution of allergen-specific IgE (sIgE) for children with atopic dermatitis in Tianjin City and provide the evidences of clinical diagnosis and treatment. Methods: A retrospective cross-sectional study was conducted to analyze the children who were suspected of atopic dermatitis and tested for serum sIgE in the Tianjin Children's Hospital from March 2021 to February 2023. Using first detection results only, a total of 1 841 serum samples were tested for twenty common allergens. The method was the enzyme-linked immune capture assay. The allergen epidemiological characteristics were statistically analyzed by Chi square test based on the children's characteristics and factors such as different sexes, ages and seasons by the mass data. Results: Among the 1 841 cases, the results showed that 1 247 (67.73%) were sensitized to at least 1 allergen-sIgE, comprising to 49.86% (918/1 841) to food allergen-sIgE and 47.96% (883/1 841) to aeroallergen-sIgE. The top three food allergens-sIgE were egg 32.10% (591/1 841), milk 25.91% (477/1 841) and wheat flour 14.61% (269/1 841); the top three positive rates of aeroallergens-sIgE were house dust 24.33% (448/1 841), alternaria 20.59% (379/1 841) and dermatophagoides farinae 14.83% (273/1 841). The positive rates of food allergens-sIgE were the highest in the 1-3 years old group (64.11%, 434/677) (χ2=122.854, P<0.001), while the positive rates of aeroallergens-sIgE were higher in the 11-14 years old group (71.26%, 62/87) (χ2=134.968, P<0.001). No seasonal difference was revealed in the overall positive rate of food allergen-sIgE and aeroallergen-sIgE (χ2=4.047, P=0.256; χ2=7.549, P=0.056). The positive rates of soybean-sIgE and milk-sIgE were the highest in summer (χ2=11.329, P=0.010; χ2=28.720, P<0.001), whereas alternaria-sIgE and mugwort-sIgE were the highest in summer and autumn, respectively (χ2=8.462, P=0.037; χ2=10.641, P=0.014). Among the 1 841 cases, 32.21% were sensitized to three or more allergens-sIgE. The sIgE concentration levels of egg, milk and house dust were mainly level 1 to 2, and the proportions of level 3 and above were all under 15%; although the positive rates of crab, shrimp, and peanut were low, the proportions of grade 3 and above were all beyond 30%. Children sensitized to alternaria, dermatophagoides farinae, mugwort, and cat dander had higher sIgE concentration levels, which were 68.07%, 49.45%, 56.57% and 47.83% respectively. Conclusions: This study can reflect the epidemic characteristics of allergen-sIgE in children with atopic dermatitis in Tianjin region to a certain extent. Allergen-sIgE positivity in patients differed by age, and there were seasonal differences and grade distribution differences in the positive rates of some allergens-sIgE. It is necessary to reasonably avoid the high-risk allergens according to the epidemiological characteristics and clinical symptoms, which provide valuable information for the prevention, diagnosis and treatment of atopic dermatitis.

Descriptive epidemiology of spotty liver disease in Australian cage-free brown egg layer chicken flocks with a scratch area

Spotty Liver Disease (SLD), caused by Campylobacter hepaticus or C. bilis infection in adult female chickens continues to emerge as a major disease problem in cage-free production systems. Free range production has become the predominant system in Australian egg production and SLD is widespread in these farms. Previous studies have identified having a scratch area as a key determinant for SLD occurrence. An Australia-wide survey of egg production flocks with scratch areas was conducted regarding SLD including 48 individual flocks. Descriptive information on the facilities and flock management practices was reported. The incidence of SLD, age of first outbreak, initial mortality rate, duration of elevated mortality, and magnitude and duration of any associated egg production decline are described. Recurrence of SLD in the same flock was also reported and discussed. Therapies applied were recorded and assessed across SLD severity and duration. SLD occurred in 66.7% of layer flocks whose facility included a scratch area. Recurrent SLD outbreaks occurred in 31% of flocks experiencing SLD. Antibiotic medication reduced duration of mortality and egg production decline. Antibiotic therapy was associated with reduced duration of mortality and a less severe and shorter duration of egg production drops compared to untreated flocks. PCR detection of C. hepaticus in cloacal swabs and house dust samples and a serological ELISA test were compared and evaluated as diagnostic aids or as possible predictors of SLD outbreaks. The ELISA showed substantial agreement with detection of C. hepaticus in cloacal swabs by PCR. Examining composite house dust samples by PCR for C. hepaticus DNA appeared to be the most convenient and cost-effective aid to diagnosis and as a putative predictor for SLD outbreaks.

Concentration, Pollution Level, and Sources of Heavy Metals from Household Dust in Ramadi City, Iraq

Researchers and environmental managers have been interested in household dust pollution because exposure to heavy metals has a detrimental effect on health. This study aims to determine heavy metals concentrations (Cd, Cr, Cu, Ni, Pb and Zn), pollution level, and their potential sources in the household dust of Ramadi City, Iraq. The dust was collected from 35 houses. The atomic absorption spectrophotometry method was used to measure concentrations of heavy metals in the dust. The following was the order of the heavy metal mean concentration: Zn &gt; Ni &gt; Cu &gt; Pb &gt; Cr &gt; Cd. These metals’ mean concentrations are below background guidelines. The descending order of the EF values for the measured metals is as follows: Ni (6.35) &gt; Cu (5.40) &gt; Pb (1.53 &gt; Cr (1.24) &gt; Zn (1.14) &gt; Cd (0.84). Results of the calculation of CF for metals measured in house dust in Ramadi City showed values of less than 1 (CF &lt; 1 indicating a minimum contamination level. Based on the results of multivariate statistics (correlation matrix, cluster and principle components analysis), sources of Cr, Cu, Ni and Zn are indoor anthropogenic sources while the sources of Cd and Pb are outdoor anthropogenic.

Exploring the Potentiality of a Plant Platform for Monoclonal Antibody Production in Veterinary Medicine.

Canine atopic dermatitis (CAD) is an allergic, inflammatory, and pruritic skin disease associated with the production of IgE antibodies against environmental allergens and mainly house dust mite allergens. This complex dermatological pathology involves Interleukin 31 (IL-31) as a central itch mediator. One of the most effective CAD treatments is a caninized monoclonal antibody (mAb) called Lokivetmab. It is produced in CHO cells and targets specifically canine IL-31 (cIL-31) and blocks its cellular messaging. This treatment has undoubtedly contributed to a breakthrough in dermatitis-related pruritus. However, its production in mammalian cells requires time-consuming procedures, high production costs, and investment. Plants are considered an emerging protein production platform for recombinant biopharmaceuticals due to their cost-effectiveness and rapidity for production. Here, we use transient expression in Nicotiana benthamiana plants to produce recombinant canine Interleukin 31 (cIL-31) and an anti-IL-31 monoclonal antibody (M1). First, we describe the production and characterization of M1 and then its activity on an IL-31-induced pruritic model in dogs compared to its commercial homolog. Dogs treated with the plant-made M1 mAb have shown similar improvements to Lokivetmab-treated ones after different challenges using canine IL-31. Furthermore, M1 injections were not associated with any side effects. These results demonstrate the safety and efficacy of this plant-made Lokivetmab biosimilar to control dogs' pruritus in a well-established model. Finally, this study shows that the plant-production platform can be utilized to produce rapidly functional mAbs and bring hope to the immunotherapy field of veterinary medicine.

Obesity Enhances Non-Th2 Airway Inflammation in a Murine Model of Allergic Asthma.

Obese patients with asthma present with aggravated symptoms that are also harder to treat. Here, we used a mouse model of allergic asthma sensitised and challenged to house dust mite (HDM) extracts to determine whether high-fat-diet consumption would exacerbate the key features of allergic airway inflammation. C57BL/6 mice were intranasally sensitised and challenged with HDM extracts over a duration of 3 weeks. The impact of high-fat-diet (HFD) vs. normal diet (ND) chow was studied on HDM-induced lung inflammation and inflammatory cell infiltration as well as cytokine production. HFD-fed mice had greater inflammatory cell infiltration around airways and blood vessels, and an overall more severe degree of inflammation than in the ND-fed mice (semiquantitative blinded evaluation). Quantitative assessment of HDM-associated Th2 responses (numbers of lung CD4+ T cells, eosinophils, serum levels of allergen-specific IgE as well as the expression of Th2 cytokines (Il5 and Il13)) did not show significant changes between the HFD and ND groups. Interestingly, the HFD group exhibited a more pronounced neutrophilic infiltration within their lung tissues and an increase in non-Th2 cytokines (Il17, Tnfa, Tgf-b, Il-1b). These findings provide additional evidence that obesity triggered by a high-fat-diet regimen may exacerbate asthma by involving non-Th2 and neutrophilic pathways.

Spatial Distribution Characteristics of Fugitive Road Dust Emissions from a Transportation Hub City (Jinan) in China and Their Impact on the Atmosphere in 2020

Road silt loading (sL) directly affects the fugitive road dust (FRD) emission factor, which is an important parameter in the study of FRD emissions. In this study, an improved collection method combined with the AP−42 method was newly developed to estimate the sL of asphalt roads in Jinan, China. The characteristics of sL in Jinan followed the order National highway (NH) &gt; Branch road (BR) &gt; Provincial highway (PH) &gt; Country highway (CH) &gt; Minor arterial (MiA) &gt; Major arterial (MaA) &gt; Urban expressway (UE) with 3.9 ± 2.5, 3.9 ± 1.9, 3.8 ± 2.8, 3.8 ± 0.9, 2.1 ± 1.4, 1.7 ± 1.2, and 1.4 ± 1.2 g/m2, respectively. The size orders of PM2.5 and PM10 emission factors are consistent with total suspended particulate (TSP). The characteristics of the TSP emission factor of FRD followed the order NH &gt; PH &gt; CH &gt; Expressway (EW) &gt; MiA &gt; BR &gt; MaA &gt; UE with 27.3, 23.4, 19.4, 13.7, 7.7, 7.4, 6.2, and 3.0 g/VKT (vehicle kilometers traveled), respectively. The annual emissions of TSP, PM10, and PM2.5 from FRD in Jinan in 2020 were about 985.2, 209.8, and 57.8 kt, respectively. Laiwu, Jiyang, and Licheng districts show the top three TSP emissions of FRD; the sum of their emissions accounts for 44.7% of the TSP emissions from FRD in Jinan. TSP emissions from municipal roads and administrative roads accounted for about 29.2% and 70.8% of the total emissions in Jinan, respectively, of which emissions from MiA accounted for the largest proportion of TSP emissions from municipal roads, contributing about 37.9%, while TSP emissions from NH made the largest contribution to TSP emissions from administrative roads, with a contribution of about 35.8%. Based on Monte Carlo simulation results using Crystal Ball, the uncertainty range of the emission inventory of FRD in Jinan ranged from −79.9 to 151.8%. In 2020, about 985,200 tons of road particulate matter in Jinan City entered the atmosphere, having an adverse effect on air quality and human health.

Assessment of metals and metalloids agglutinated to airborne suspended particulate matter in selected plant species during the pre-and post-monsoon in the urban area

The elemental accumulation has emerged as a major environmental concern due to various anthropogenic sources such as vehicles, road dust, and industrial activities, contributing to the agglutination of elements to airborne Suspended Particulate Matter (SPM). SPM-bound elements accumulate on plant surfaces impact air quality and human health due to their noxiousness. Therefore, plants' ability to capture and mitigate air pollutants plays a crucial role in urban areas. This study aimed to investigate the levels and distribution of twenty-six elements, comprised of heavy metals (Cd, Pb, Cr, Cu Zn, Co, Ni, Fe, Mn, Ag, Mo, V, Ga, and Bi), light metals (B, As, Te, and Se), and metalloids (Al, Li, Sr, K, Mg, Na, Ca, and Ba) accumulated on the surface and inside the leaves of dominant plant species during the pre-and post-monsoon at six categorized (commercial, traffic-prone, residential, educational, greenbelt and industrial areas) locations in Delhi, India. In addition, the Metal Accumulation Index (MAI) was determined, and the statistical analysis was conducted using two-way ANOVA, Principal Component Analysis (PCA), and Hierarchical Cluster Analysis (HCA). In the pre-and post-monsoon, two-way ANOVA revealed significant differences (P < 0.05) in metal concentrations. During the pre-monsoon plants exhibited the highest metal accumulation (∼21%) at the Anand Vihar (commercial) in Delhi, with the maximum average concentrations of Cr (118.25 mg/kg), Cu (204.38 mg/kg), Zn (293.27 mg/kg), and Fe (2721.17 mg/kg). Ficus benghalensis L exhibited the maximum 213.73 MAI at the Anand Vihar in the pre-monsoon. Ni and Cr indicated the highest correlation (P < 0.05, r = 0.82) in the PCA test. HCA test revealed similarity (∼87.7%) at ITO (traffic-prone) and Okhla Phase-2 (industrial) in F. religiosa regarding metal concentration patterns. Findings highlighted seasonal elemental pollutants uptake dynamics of plant species and explored species-specific metal accumulation, revealing potential implications of metal-tolerant plants for urban greenbelt.

Gonadal sex and chromosome complement influence the gut microbiome in a mouse model of allergic airway inflammation.

Evidence abounds that gut microbiome components are associated with sex disparities in the immune system. However, it remains unclear whether the observed sex disparity in asthma incidence is associated with sex-dependent differences in immune-modulating gut microbiota, and/or its influence on allergic airway inflammatory processes. Using a mouse model of house dust mite (HDM)-induced allergic inflammation and the four core genotypes (FCGs) model, we have previously reported sex differences in lung inflammatory phenotypes. Here, we investigated associations of gut microbiomes with these phenotypes by challenging FCG mice [mouse with female sex chromosome and male gonad (XXM), mouse with female sex chromosome and female gonad (XXF), mouse with male sex chromosome and male gonad (XYM), and mouse with male sex chromosome and female gonad (XYF); n = 7/group] with HDM (25 μg) or PBS intranasally for 5 wk and collecting fecal samples. We extracted fecal DNA and analyzed the 16S microbiome via Targeted Metagenomic Sequencing. We compared α and β diversity across genotypes and assessed the Firmicutes/Bacteroidetes (F/B) ratio. When comparing baseline and after exposure for the FCG, we found that the gut F/B ratio was only increased in the XXM genotype. We also found that α diversity was significantly increased in all FCG mice upon HDM challenge, with the highest increase in the XXF, and the lowest in the XXM genotypes. Similarly, β diversity of the microbial community was also affected by challenge in a gonad- and chromosome-dependent manner. In summary, our results indicated that HDM treatment, gonads, and sex chromosomes significantly influence the gut microbial community composition. We concluded that allergic lung inflammation may be affected by the gut microbiome in a sex-dependent manner involving both hormonal and genetic influences.NEW & NOTEWORTHY Recently, the gut microbiome and its role in chronic respiratory disease have been the subject of extensive research and the establishment of its involvement in immune functions. Using the FCG mouse model, our findings revealed the influence of gonads and sex chromosomes on the microbial community structure before and after exposure to HDM. Our data provide a potential new avenue to better understand mediators of sex disparities associated with allergic airway inflammation.

High Serum Allergen-Specific IgE of House Dust Mite in Predicting the Risk of Comorbidity in Children with Allergic Conjunctivitis.

To investigate the patterns of allergens in allergic conjunctivitis (AC) and the association with allergic comorbidity. This retrospective cross-sectional study enrolled 2972 children with AC. Clinical data, including sex, age, allergic comorbidities (allergic asthma, allergic rhinitis, and atopic dermatitis), and serum allergen-specific immunoglobulin E (sIgE), were collected from the electronic medical record (EMR). The categorical variables were compared with the chi-square test. The characteristics of allergens in children of different ages and comorbidities were analyzed by trend chi-square. The sensitivity level of HDM associated with AC and comorbidities was assessed by odds ratios (ORs) with 95% confidence intervals of logistic regression analysis. A total of 2972 children (2015 boys and 957 girls) with AC were included in the study. The mean age was 3.78 (0.5~12) years. The most common allergen was house dust mite(HDM) (43.41%). With age, the positive rate for inhaled allergens gradually increased, and the positive rate for ingested allergens decreased. Withthe number of comorbiditiesincreasing, the positive rates of sensitization were 38.33%, 74.51%, 80.72%, and 89.05%, and the incidence of polysensitization was 44.66%, 56.48%, 59.54%, and 74.59%, respectively. With the increase of HDM-sIgE level, the number of comorbidities and the risk increased gradually. HDM is the most common allergen in AC children of different ages. High levels of HDM-sIgE may be a predictor for allergic comorbidities. Children with polysensitization and high levels of HDM sIgE will be an important target population for future intervention in other allergy-related disease prevention.

Understanding the dynamics of microplastics transport in urban stormwater runoff: Implications for pollution control and management

The transport of microplastics (MPs) from urban environments to water resources via stormwater runoff poses significant concerns due to its adverse impacts on water safety and aquatic ecosystems. This study presents a modeling approach aimed at understanding the transport mechanisms of MPs in an urban residential setting, considering settling and buoyant MPs. To consider the effect of MP shapes, the settling velocity of various settling MPs in shapes of fibers, films, and fragments was calculated. Using an analogy of sediment transport, a Rouse number criterion was used to analyze the transport of MPs. For buoyant MPs, it was assumed that they transport as wash-load as soon as they float in the water and the travel time for them to reach the storm drain was determined. The calculation of settling velocity revealed the influence of shape on the settling velocity of MPs was particularly pronounced as the equivalent diameter of the MPs increased. The transport mechanism for the smallest settling MPs, irrespective of their shapes, density, and depth of flow, was wash-load. However, for larger MPs, the shape and size distribution of settling MPs, along with the depth of flow and slope significantly influenced their transport mechanisms compared to sediment particles. The influence of weathering on the MPs’ transport mechanisms depended on their sizes and shapes. The site-specific characteristics, including slope and surface friction, significantly influenced the velocity of stormwater runoff and, consequently, the extent of MP transport during rain events. Moreover, an evaluation of the transport mechanism of settling MPs was conducted using the reported field data on MP abundance in road dust collected from residential and traffic sites. This study underscores the complexity of MP transport dynamics and provides a foundation for developing targeted strategies to mitigate MP pollution in urban environments.

The disrupted molecular circadian clock of monocytes and macrophages in allergic inflammation.

Macrophage dysfunction is a common feature of inflammatory disorders such as asthma, which is characterized by a strong circadian rhythm. We monitored the protein expression pattern of the molecular circadian clock in human peripheral blood monocytes from healthy, allergic, and asthmatic donors during a whole day. Monocytes cultured of these donors allowed us to examine circadian protein expression in human monocyte-derived macrophages, M1- and M2- polarized macrophages. In monocytes, particularly from allergic asthmatics, the oscillating expression of circadian proteins CLOCK, BMAL, REV ERBs, and RORs was significantly altered. Similar changes in BMAL1 were observed in polarized macrophages from allergic donors and in tissue-resident macrophages from activated precision cut lung slices. We confirmed clock modulating, anti-inflammatory, and lung-protective properties of the inverse ROR agonist SR1001 by reduced secretion of macrophage inflammatory protein and increase in phagocytosis. Using a house dust mite model, we verified the therapeutic effect of SR1001 in vivo. Overall, our data suggest an interaction between the molecular circadian clock and monocytes/macrophages effector function in inflammatory lung diseases. The use of SR1001 leads to inflammatory resolution in vitro and in vivo and represents a promising clock-based therapeutic approach for chronic pulmonary diseases such as asthma.

Seasonal toxicity of urban road dust in runoff process-studies in Poland

Urban road dust (URD) is one of the most important non-point sources of pollution in agglomerations. The aim of this study was to assess the seasonal toxic effects of URD runoff in two regions of Poland. The concentrations of elements in URD and leachate were studied. The impact of pollutants in URD runoff on water organisms was evaluated using Daphtoxkit F and Rotoxkit F (LC50). The acute toxicity tests for crustaceans and rotifers were selected as the response of these taxa reflects the impact on zooplankton, a key component of aquatic ecosystem and the basis of most food webs. The concentrations of elements were found to vary depending on the site, although URD samples collected in Katowice agglomeration (Upper Silesia) had higher values of elements (Mn, Cu, Zn, As) compared to Wrocław (Lower Silesia). The concentrations of Mn, Zn, As, Cr, and Mg in water-soluble fraction of URD were higher in summer and winter in the Upper Silesia region due to rainwater runoff resulting from traffic, industries, post-industrial waste, and the presence of old heating systems. When comparing the content of elements in the water-soluble fraction between seasons, Zn, As, Cr, and Al concentrations were slightly higher in winter. The highest mortality of Daphnia magna and Brachiouns calyciflorus was observed in URD from both agglomerations in winter. However, the mortality is likely due to the concentration of elements or/and the coexistence of an unknown compound or a synergistic effect of the studied elements. This study highlights the alarming seasonal sources of elements in URD runoff, which will directly enter the food chain and affect the entire ecosystem, and human health.

Concentration level of radionuclides in road dust in the urban atmosphere of two cities of Pakistan

This research focused on measuring the concentration of particulate matter (PM2.5) aerosols and radionuclides (226Ra, 232Th, and 40K) in road dust collected using glass fiber filters from busy roads and populated areas in Islamabad and Rawalpindi, Pakistan. The measured concentrations ranged between 551.0 and 2025.7 µgm−3 for PM2.5 aerosols, 32.4 and 54.7 mBqm−3 for 226Ra, 15.8 and 26.8 mBqm−3 for 232Th, and 239.0 and 316.4 mBqm−3 for 40K. The study found that PM2.5 levels surpassed the WHO’s recommended limit of 25 µgm−3. Additionally, concentrations of 226Ra and 232Th were considerably higher than the global average values of 1 and 0.5 µBqm−3, respectively, in dust. The total inhalation effective dose in all samples exceeded the ICRP’s acceptable limit of 2 mSvy-1, except in the infant and 1-year-old age groups (considering F, M, and S-type modes). Notably, 232Th was identified as the primary contributor, accounting for 36.5 % to 99.4 % of the total inhalation effective dose.

Source identification of ultrafine particulate matter and ammonia emissions in the metropolitan area

Previous studies have estimated the sources of particulate matter in the atmosphere. Among these, studying the sources of secondary aerosols harmful to human health is important. However, there is a lack of research on ammonia (NH3), a precursor to secondary aerosol formation. This study uses positive matrix factorization (PMF) model and conditional bivariate probability function (CBPF) model to estimate the sources of particulate matter and ammonia. The results showed that about 40% of the PM2.5 mass at both sites was attributable to secondary aerosol. To estimate the emission sources of ammonia that contribute to the generation of secondary aerosols, CBPF was utilized to model and compare the emission characteristics of categorized pollution sources and ammonia, and it was found that SMA had similar emission trends to industry, road dust, oil combustion, and biomass combustion, while GRA had similar emission trends to oil combustion and vehicle (diesel). Considering the results from these two regions, ammonia in the metropolitan area is more likely to be emitted from daily activities than from long distances. The study results demonstrate the major role of secondary aerosols on ambient PM2.5 concentrations and can help develop effective management strategies and policies for air pollution mitigation.

Point-of-Care Fluorescence Biosensing System for Rapid Multi-Allergen Screening.

With the steady increase in allergy prevalence worldwide, there is a strong need for novel diagnostic tools for precise, fast, and less invasive testing methods. Herein, a miniatured fluorescence-based biosensing system is developed for the rapid and quantitative detection of allergen-specific immunoglobulin-E. An antibody-based fluorescence assay in a microfluidic-patterned slide, combined with a custom-made portable fluorescence reader for image acquisition and user-friendly software for the data analysis, enables obtaining results for multiple allergens in just ~1 h with only 80 μL of blood serum. The multiplexed detection of common birch, timothy grass, cat epithelia, house dust mite, and dog epithelia shows quantitative IgE-mediated allergic responses to specific allergens in control serum samples with known total IgE concentration. The responses are verified with different control tests and measurements with a commercial fluorescence reader. These results open the door to point-of-care allergy screening for early diagnosis and broader access and for large-scale research in allergies.

TRIM47 promotes HDM-induced bronchial epithelial pyroptosis by regulating NEMO ubiquitination to activate NF-κB/NLRP3 signaling.

Asthma is an inflammatory disease. Airway epithelial cell pyroptosis and cytokine secretion promote asthma progression. Tripartite motif 47 (TRIM47)belongs to the E3 ubiquitin ligase family and is associated with apoptosis and inflammation in a range of diseases. However, the role of TRIM47 in asthma has not been explored. In this study, the human bronchial epithelial cell line BEAS-2B was treated with house dust mite (HDM) and TRIM47 expression was detected by RT-qPCR and Western blot. After transfection with TRIM47 interfering and overexpressing plasmids, the synthesis and secretion of cytokines, as well as pyroptosis-related indicators, were examined.Nuclear factor kappa-B (NF-κB)pathway proteins and nod-like receptor protein 3 (NLRP3)inflammasome were measured to explore the mechanism of TRIM47 action. In addition, the effect of TRIM47 on the level of NF-κB essential modulator (NEMO) ubiquitination was detected by an immunoprecipitation assay. The results showed that TRIM47 was upregulated in HDM-induced BEAS-2B cells and that TRIM47 mediated HDM-induced BEAS-2B cell pyroptosis and cytokine secretion. Mechanistically, TRIM47 promoted the K63-linked ubiquitination of NEMO and facilitated NF-κB/NLRP3 pathway activation. In conclusion, TRIM47 may promote cytokine secretion mediating inflammation and pyroptosis in bronchial epithelial cells by activating the NF-κB/NLRP3 pathway. Therefore, TRIM47 may be a potential therapeutic target for HDM-induced asthma.

Characterization of Road Dust in Delhi: Heavy Metal Analysis, Health Risks, and Sustainability Implications

Characterization of Road Dust in Delhi: Heavy Metal Analysis, Health Risks, and Sustainability Implications

Mesenchymal stromal cells dampen trained immunity in house dust mite-primed macrophages expressing human macrophage migration inhibitory factor polymorphism

BackgroundTrained immunity results in long-term immunological memory, provoking a faster and greater immune response when innate immune cells encounter a secondary, often heterologous, stimulus. We have previously shown that house dust mite (HDM)-induced innate training is amplified in mice expressing the human macrophage migration inhibitory factor (MIF) CATT7 functional polymorphism. AimThis study investigated the ability of mesenchymal stromal cells (MSCs) to modulate MIF-driven trained immunity both in vitro and in vivo. MethodsCompared with wild-type mice, in vivo HDM-primed bone marrow-derived macrophages (BMDMs) from CATT7 mice expressed significantly higher levels of M1-associated genes following lipopolysaccharide stimulation ex vivo. Co-cultures of CATT7 BMDMs with MSCs suppressed this HDM-primed effect, with tumor necrosis factor alpha (TNF-α) being significantly decreased in a cyclooxygenase 2 (COX-2)-dependent manner. Interestingly, interleukin 6 (IL-6) was suppressed by MSCs independently of COX-2. In an in vitro training assay, MSCs significantly abrogated the enhanced production of pro-inflammatory cytokines by HDM-trained CATT7 BMDMs when co-cultured at the time of HDM stimulus on day 0, displaying their therapeutic efficacy in modulating an overzealous human MIF-dependent immune response. Utilizing an in vivo model of HDM-induced trained immunity, MSCs administered systemically on day 10 and day 11 suppressed this trained phenomenon by significantly reducing TNF-α and reducing IL-6 and C-C motif chemokine ligand 17 (CCL17) production. ConclusionsThis novel study elucidates how MSCs can attenuate an MIF-driven, HDM-trained response in CATT7 mice in a model of allergic airway inflammation.