True random number generators (TRNGs) play a very important role in modern cryptographic systems. Field-programmable gate arrays (FPGAs) form an ideal platform for hardware implementations of many of these security algorithms. In this brief, we present a highly efficient and tunable TRNG based on the principle of beat frequency detection , specifically for Xilinx -FPGA-based applications. The main advantages of the proposed TRNG are its on-the-fly tunability through dynamic partial reconfiguration to improve randomness qualities. We describe the mathematical model of the TRNG operations and experimental results for the circuit implemented on a Xilinx Virtex-V FPGA. The proposed TRNG has low hardware footprint and built-in bias elimination capabilities. The random bitstreams generated from it pass all tests in the NIST statistical testsuite.