Soil organic carbon (SOC) is a significant component that plays an important role in soil fertility and crop sustainable production. This study investigated SOC in soil profiles of rice-corn rotation and corn monocropping using a stable carbon isotope of SOC (δ13CSOC) technique. The soil samples were collected from two plots of rice-corn rotation and corn monocropping fields. Soil sampling was conducted in Tak province, involving the collection of 25 soil cores per plot down to a depth of 50 cm. Each soil profile was further segmented into ten depth layers: 0-5 cm, 5-10 cm, 10-15 cm, 15-20 cm, 20-25 cm, 25-30, 30-35, 35-40, 40-45, and 45-50 cm. The collected soil samples were then subjected to air drying, pulverization, and sieving through a 200-mesh sieve. The δ13CSOC in rice-corn rotation ranged from -28.41‰ to -23.72‰ while corn monocropping was -24.59‰ to -15.83‰. The results revealed that the values of δ13CSOC for each soil profile gradually increased with soil depth. Since δ13CSOC levels were negatively correlated to SOC. Therefore, SOC decreased with increasing soil depth in both the rice-corn and corn fields. However, significantly higher SOC contents were observed in rice-corn rotation rather than in corn monocropping fields (p < 0.05). SOC contents were higher in soil with rice straw retention than without rice residues. The rotation of rice-corn practice could potentially retain organic carbon in soil better than corn monocropping.
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