Stratified Water Bodies Research Articles

Metagenomics and Stable Isotopes Uncover the Augmented Sulfide-Driven Autotrophic Denitrification in a Seasonally Hypoxic, Sulfate-Abundant Reservoir.

The mechanism governing sulfur cycling in nitrate reduction within sulfate-rich reservoirs during seasonal hypoxic conditions remains poorly understood. This study employs nitrogen and oxygen isotope fractionation in nitrate, along with metagenomic sequencing to elucidate the intricacies of the coupled sulfur oxidation and nitrate reduction process in the water column. In the Aha reservoir, a typical seasonally stratified water body, we observed the coexistence of denitrification, bacterial sulfide oxidation, and bacterial sulfate reduction in hypoxic conditions. This is substantiated by the presence of abundant N/S-related genes (nosZ and aprAB/dsrAB) and fluctuations in N/S species. The lower 15εNO3/18εNO3 ratio (0.60) observed in this study, compared to heterotrophic denitrification, strongly supports the occurrence of sulfur-driven denitrification. Furthermore, we found a robust positive correlation between the metabolic potential of bacterial sulfide oxidation and denitrification (p < 0.05), emphasizing the role of sulfide produced via sulfate reduction in enhancing denitrification. Sulfide-driven denitrification relied on ∑S2- as the primary electron donor preferentially oxidized by denitrification. The pivotal genus, Sulfuritalea, emerged as a central player in both denitrification and sulfide oxidation processes in hypoxic water bodies. Our study provides compelling evidence that sulfides assume a critical role in regulating denitrification in hypoxic water within an ecosystem where their contribution to the overall nitrogen cycle was previously underestimated.

Analysis of nitrogen migration and transformation in the typical deep and large reservoir of the Lancang River — Evidence from nitrogen and oxygen isotopes

The environmental effect resulting from dam construction and the formation of large reservoirs has long been a topic of significant concern. While current research at a macro scale provides a well understanding regarding the evolution of nitrogen nutrient status in river-reservoir ecosystems, the vertical migration and transformation characteristics of nitrogen in reservoirs under changing runoff remain less defined. This study centers on a typical deep reservoir along the Lancang River-Nuozhadu (NZD) Reservoir in April and December. The monitoring reveals a distinct vertical stratification phenomenon in the NZD Reservoir in the vertical profile in April, which can be categorized into a mixed layer (ML) (0–5 m), a thermocline layer (TL) (5–40 m), and an isothermal layer (IL) (>40 m) based on the water temperature. However, little stratification in the profile is occurred in December. The results of nitrogen and oxygen isotopes in April indicate that the biochemical process of nitrogen in the NZD Reservoir is predominantly driven by nitrification. However, different nitrogen biochemical processes are coupled in various stratified regions in the vertical direction. In the ML, simultaneous assimilation of ammonia nitrogen by phytoplankton occurs. In the TL, there is a process of oxidation and decomposition of particulate organic nitrogen due to a significant stratification effect and an extended hydraulic retention time. In the region of minimal dissolved oxygen (30–40 m), simultaneous denitrification may take place. In December, the main process is nitrogen assimilation in reservoir surface (ML). Statistical analysis of environmental factors highlights water temperature (T) and dissolved oxygen (DO) as crucial environmental factors influencing nitrogen migration and transformation within the reservoir. This study suggests that in deep reservoirs experiencing vertical thermal stratification, the characteristics of DO and nitrogen migration and transformation exhibit significant vertical spatial heterogeneity. This phenomenon may lead to diverse ecological environmental effects. Future efforts should focus on enhancing the fine monitoring of stratified water bodies in deep and large reservoirs and analyzing the ecological environmental effects of material cycling in greater detail.

Integrating experiments with modeling to understand key bacterial taxa dynamics after episodic mixing of a stratified water column

Hydraulic mixing of stratified reservoirs homogenizes physicochemical gradients and microbial communities. This has potential repercussions for microbial metabolism and water quality, not least in dams and hydraulically controlled waters. A better understanding of how key taxa respond to mixing of such stratified water bodies is needed to understand and predict the impact of hydraulic operations on microbial communities and nutrient dynamics in reservoirs. We studied taxa transitions between cyanobacteria and sulfur-transforming bacteria following mixing of stratified water columns in bioreactors and complemented the experimental approach with a biogeochemical model. Model predictions were consistent with experimental observations, suggesting that stable stratification of DO is restored within 24 h after episodic and complete mixing, at least in the absence of other more continuous disturbances. Subsequently, the concentration of S2− gradually return to pre-mixing states, with higher concentration at the surface and lower in the bottom waters, while the opposite pattern was seen for SO42−. The total abundance of sulfate-reducing bacteria and phototrophic sulfur bacteria increased markedly after 24h of mixing. The model further predicted that the rapid re-oxygenation of the entire water column by aeration will effectively suppress the water stratification and the growth of sulfur-transforming bacteria. Based on these results, we suggest that a reduction of thermocline depth by optimal flow regulation in reservoirs may also depress sulfur transforming bacteria and thereby constrain sulfur transformation processes and pollutant accumulation. The simulation of microbial nutrient transformation processes in vertically stratified waters can provide new insights about effective environmental management measures for reservoirs.

Molecular Composition Evolution of Dissolved Organic Matter With Water Depth in Prydz Bay of East Antarctic: Carbon Export Implications

AbstractThis study analyzes the molecular composition of dissolved organic matter (DOM) in Prydz Bay by Fourier Transform Ion Cyclotron Resonance mass spectrometry to probe the carbon sequestration capacity in the continental shelf system. Concentrations of particulate organic carbon (POC), particulate nitrogen and dissolved organic carbon (DOC) with water depth show that POC could be mainly decomposed into DOC and/or microbially degraded. Highly labile DOC is further degraded and remineralized by microorganisms within the upper 200 m, as evidenced by a downward enrichment of 13CPOC and increases in the average molecular weight, oxygen atom number (O) and double bond equivalents of DOM molecules, indicating that biodegradation is the main driver for particulate organic matter and DOM evolution with water depth. Semi‐quantitative calculation demonstrates that ∼83% of POC was transformed to DOC as well as dissolved inorganic carbon (DIC), and ∼30% of DOC further to DIC via microbial degradation within the upper 200 m in summer, resulting in a relatively low total organic carbon content in sediments of Prydz Bay. The newly transformed DIC and residue DOC can be preserved in the deep layer due to the formation of well stratified and stable water body in summer of Prydz Bay, ultimately entering the regional circulation system instead of being released back into the atmosphere. This could be one of the most important processes determining the atmosphere CO2 uptake in the continental shelf system of Southern Ocean.

Metalimnion as an ecotone in stratified freshwater lakes: A case study on benthic testate amoebae in Lake Valdayskoe

Abstract Testate amoebae are widely used as bioindicators in the study of modern ecotopes and as proxies in palaeoecological reconstructions of lacustrine environments. The metalimnion with thermocline in stratified water bodies strongly affects the species structure of a number of aquatic animals and often plays a role as an ecotone for pelagic animals, but our knowledge on the influence of the metalinmion on benthic organisms, especially on testate amoebae, is still limited. This study aims to describe the influence of the metalimnion and its thermocline on benthic testate amoebae assemblages along a temperature gradient in the stratified freshwater Lake Valdayskoe. Twenty‐eight samples from nine sites were collected along the transect starting in the littoral zone, passing through a long metalimnion and its thermocline and finishing in the hypolimnion on the bottom slope. We identified four species assemblages, six non‐attributed samples and five morphotrait compositions in the transect, including those corresponding to the area inside the metalimnetic zone. We observed similarities in the transitions in benthic species assemblages, morphotype compositions, and species and morphotrait structures from shallow to deep water, and two shifts corresponded well with the upper and lower limnological boundaries of the metalimnion. The combination of the observed biological and hydrological characteristics suggests that the metalimnion plays a role of an ecotone between shallow and deep water in stratified freshwater lakes, and maintains the integrity and sustainability of benthic communities of testate amoebae. We explored a multivariate approach to demonstrate that the combination of highly abundant species and dominant morphotypes may be an efficient indicator of related environmental conditions. Thus, the high abundances of Cylindrifflugia acuminata, Centropyxis aculeata, Difflugia lacustris, D. linearis and C. discoides in combination with the dominance of elongate‐spiky and flattened‐spiky shells are closely related to the littoral zone with sandy sediments covered by many macrophytes. The combination of a high abundance of D. petricola and D. penardi, and the dominance of pyriform, necked and oval shells are closely related to deep depth. The studied species and morphotrait transition from littoral to deep water provides additional information about the morphological adaptations of testaceans, and about the processes of forming and maintaining benthic testate amoebae assemblages along the depth gradient. The described species and morphotrait assemblages related to specific water depths may be useful for palaeoreconstructions.

Dissolved organic matter (DOM) is independently stratified in thermally stratified water bodies

Thermal stratification often occurs in deep-water bodies, including oceans, lakes, and reservoirs. Dissolved organic matter (DOM) plays a critical role in regulating the dynamics of aquatic food webs and water quality in aquatic ecosystems. In the past, thermal stratification boundaries have been sometimes used exclusively to analyze the vertical distribution of DOM in thermally stratified water bodies. However, the validity of this practice has been challenged. Currently, there is limited understanding of the formation mechanism and stratification of the vertical distribution of DOM in thermally stratified water bodies, which hinders the analysis of the interactions between DOM and vertical aquatic ecological factors.To address this gap, we conducted a comprehensive study to extensively collect the vertical distribution of DOM in thermally stratified water bodies and identify the primary factors influencing this distribution. We found that DOM was independently stratified in thermally stratified water bodies (including two cases in unstratified water bodies), and that the formation mechanisms and statuses of DOM stratification were different from those of thermal stratification. The boundaries and numbers of DOM stratification were generally inconsistent with those of thermal stratification. Therefore, it is more accurate to divide DOM into different layers according to its own vertical profile, and analyze DOM characteristics of each layer based on its own stratification instead of thermal stratification. This study sheds light on the relationship between DOM and thermal stratification and provides a novel approach for analyzing DOM vertical distribution characteristics and their impact on aquatic ecosystems. This finding also holds significant implications for the design and implementation of environmental management programs aimed at preserving the health and functionality of aquatic ecosystems.

Computationally characterizing the diffusive boundary layer in lakes and reservoirs

PurposeHypolimnetic hypoxia has become increasingly prevalent in stratified water bodies in recent decades due to climate change. One primary sink of dissolved oxygen (DO) is sediment oxygen uptake (JO2\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${J}_{{O}_{2}}$$\\end{document}). On the water side of the sediment–water interface (SWI), JO2\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${J}_{{O}_{2}}$$\\end{document} is controlled by a diffusive boundary layer (DBL), a millimeter-scale layer where molecular diffusion is the primary transport mechanism. In previous studies, the DBL was determined by visual inspection, which is subjective and time-consuming.Material and methodsIn this study, a computational procedure is proposed to determine the SWI and DBL objectively and automatically. The procedure was evaluated for more than 300 DO profiles in the sediment of three eutrophic water bodies spanning gradients of depth and surface area. Synthetic DO profiles were modeled based on sediment characteristics estimated by laboratory experiments. The procedure was further verified adopting the synthetic profiles.Results and discussionThe procedure, which was evaluated for both measured and synthetic DO profiles, determined the SWI and DBL well for both steady and non-steady state DO profiles. A negative relationship between DBL thickness and aeration rates was observed, which agrees with existing literatures.ConclusionsThe procedure is recommended for future studies involving characterizing DBL to improve efficiency and consistency.

Simultaneous sensing profiles of beam attenuation coefficient and volume scattering function at 180° using a single-photon underwater elastic-Raman lidar.

Lidar has emerged as a promising technique for vertically profiling optical parameters in water. The application of single-photon technology has enabled the development of compact oceanic lidar systems, facilitating their deployment underwater. This is crucial for conducting ocean observations that are free from interference at the air-sea interface. However, simultaneous inversion of the volume scattering function at 180° at 532 nm (βm) and the lidar attenuation coefficient at 532 nm (K l i d a r m) from the elastic backscattered signals remains challenging, especially in the case of near-field signals affected by the geometric overlap factor (GOF). To address this challenge, this work proposes adding a Raman channel, obtaining Raman backscattered profiles using single-photon detection. By normalizing the elastic backscattered signals with the Raman signals, the sensitivity of the normalized signal to variations in the lidar attenuation coefficient is significantly reduced. This allows for the application of a perturbation method to invert βm and subsequently obtain the K l i d a r m. Moreover, the influence of GOF and fluctuations in laser power on the inversion can be reduced. To further improve the accuracy of the inversion algorithm for stratified water bodies, an iterative algorithm is proposed. Additionally, since the optical telescope of the lidar adopts a small aperture and narrow field of view design, K l i d a r m tends to the beam attenuation coefficient at 532 nm (cm). Using Monte Carlo simulation, a relationship between cm and K l i d a r m is established, allowing cm derivation from K l i d a r m. Finally, the feasibility of the algorithm is verified through inversion error analysis. The robustness of the lidar system and the effectiveness of the algorithm are validated through a preliminary experiment conducted in a water tank. These results demonstrate that the lidar can accurately profile optical parameters of water, contributing to the study of particulate organic carbon (POC) in the ocean.

Provenance, Sedimentary Environment, Tectonic Setting, and Uranium Mineralization Implications of the Yaojia Formation, SW Songliao Basin, NE China

The SW Songliao Basin is an extremely significant part of the giant sandstone uranium metallogenic belt in northern China. The Yaojia Formation is the most significant ore-bearing layer in the region. However, the poorly constrained sedimentology of the Yaojia Formation has substantially hindered the understanding of the basin and the exploration of uranium deposits within it. To determine the sedimentology, provenance, and tectonic setting of the Yaojia Formation in the study area, we conducted petrography, whole-rock geochemical analysis, and electron probe research. Based on the results of the study, it appears that the Yaojia Formation sandstone is predominantly composed of lithic sandstone and feldspar lithic sandstone. Uranium exists in two forms: as independent minerals and as adsorption uranium. Pitchblende is the most common independent uranium mineral, with small amounts of coffinite also occurring. The ratios of Sr/Ba, V/(V+Ni), V/Cr, Ni/Co, and (Cu+Mo)/Zn of the samples indicate that the Yaojia Formation was deposited in a sub- to oxygen-rich freshwater environment with a moderately stratified bottom water body and smooth circulation. The geochemical characteristics of the Yaojia Formation sandstones imply that they are primarily derived from felsic igneous rocks in the upper continental crust in active continental margin and continental island arc environments. According to geochemistry and previous detrital zircon U-Pb chronology studies, the Mesozoic and Late Paleozoic felsic igneous rocks of the southern Great Xing’an Mountains are the principal sources of the Yaojia Formation in the SW Songliao Basin. Besides providing sediments for the study area, the uranium-rich felsic igneous rocks in the source areas also represent a long-term, stable, and ideal source of uranium, suggesting substantial potential for uranium exploration in the study area.

Effects of Algal Extracellular Polysaccharides on the Formation of Filamentous Manganese Oxide Particles in the Near-Bottom Layer of Lake Biwa.

Filamentous manganese (Mn) oxide particles, which occur in the suboxic zone of stratified waterbodies, are important drivers of diverse elemental cycles. These particles are considered to be bacteriogenic; despite the importance of biogeochemical implications, however, the environmental factor responsible for their formation has not been identified. The aim of this study was to demonstrate the involvement of algal extracellular polysaccharides in Mn oxide particle formation. Based on this study of laboratory cultures of a model Mn(II)-oxidizing bacterium, the supply of algal extracellular mucilage was shown to stimulate Mn(II) oxidation and thus the production of filamentous Mn oxide particles. This observation was consistent with the results obtained for naturally occurring particles collected from a near-bottom layer (depth of approximately 90 m) in the northern basin of Lake Biwa, Japan, that is, most Mn particles resembling δ-MnO2 were associated with an extracellular mucilage-like gelatinous matrix, which contained dead algal cells and was lectin-stainable. In the lake water column, polysaccharides produced by algal photosynthesis sank to the bottom layer. The analysis of the quality of water samples, which have been collected from the study site for 18 years, reveals that the annual average total phytoplankton biovolume in the surface layer correlates with the density of filamentous Mn particles in the near-bottom layer. Among different phytoplankton species, green algae appeared to be the key species. The results of this study suggest that algal extracellular polysaccharides serve as an important inducer for the formation of filamentous Mn oxide particles in the near-bottom layer of the northern basin of Lake Biwa.

Development of subsurface chlorophyll maximum layer and its contribution to the primary productivity of water column in a large subtropical reservoir

The phenomenon of subsurface chlorophyll maximum (SCM) layer emerging at a certain water depth is commonly found in stratified water bodies. Also, it is a crucial contributing region to the primary productivity of the water column. Currently, there is a lack of concern about the occurrence of SCM phenomena in studies targeting inland water bodies such as natural lakes and artificial reservoirs. This led to a significant underestimation of the level of primary productivity in these water bodies and their trophic state. In this study, a subtropical reservoir (the Xinanjiang Reservoir, XAJR) was investigated, to understand the characteristics of SCM layer in deep-large reservoir and its contribution to the primary productivity of the water column. Water sampling were conducted from September 2020 to August 2021, and in September 2022. Buoy station data for this reservoir between 2019 and 2021 were also collected. Based on the detailed observations of the water column profile in riverine area (X1), transitional area (X2), and central area (X3 and X4) of this reservoir, it was found that there was an obvious SCM phenomenon, which was closely related to the characteristics of seasonal thermal stratification. The SCM layer of XAJR appeared at depth around 3–5 m underwater from May to August, and as the thermal stratification strength increased, so did the depth and thickness of the SCM layer. It was estimated that gross primary productivity of euphotic layer of XAJR ranged from 347.9 to 4508.6 mgC·m−2·d−1. The average primary productivity level of the SCM layer reached 1411.7mgC·m−2·d−1, accounting for about 40–90% of the gross primary productivity of euphotic layer. This study contributes to a better understanding of the factors influencing changes in the development of the SCM layer in large reservoirs, as well as its critical role in the inland water carbon cycle.

Seasonal and inter-annual sedimentation in meromictic Lake Shira (Siberia, Russia) during disturbance of meromixis

Detailed studies of processes of sediment-record formation are necessary for accurate sediment-derived paleo environmental reconstructions. In order to understand the deposition of sediments in meromictic Lake Shira (southern Siberia, Russia), we conducted a multi-year seasonal sediment trap study of sediment fluxes of chemical elements, organic and inorganic carbon, total nitrogen and photosynthetic pigments for a period from 2012 to 2017. In 2015 and 2016, a rare event of transition from meromixis (i.e. long-term hypolimnetic anoxia) to holomixis was observed, which was accompanied by the temporary disappearance of hydrogen sulfide from the water column in spring and a decrease in hydrogen sulfide in other seasons compared to the meromictic state. In general, we did not reveal any noticeable differences between the meromictic and holomictic states in the composition of sediments, except for okenone. The content of okenone, a specific carotenoid of purple sulfur bacteria, decreased in traps during the holomictic state and did not considerably increase two years after the meromixis restoration. Using a freeze-corer, we observed that the content of okenone in the uppermost 3-mm layer of sediments also decreased markedly from 2013 to 2017, as well as in traps. The sedimentation flux of molybdenum reflected the seasonality of hydrogen sulfide content in the water column, and it was maximal in winter, in contrast to all other sediment components. Other components reflected the seasonality of organic production and terrigenous inputs, with the maximum in late summer and autumn. An increase in the sedimentation rate of terrigenous elements in the autumn of 2012 was supposedly caused by the anomalous amount of precipitation in August 2012. Since detailed inter-annual observations of sedimentation processes are not often found in limnology, we hope that our results will be useful for reconstructing regional climate and, further, the understanding of sediment dynamics of stratified temperate water bodies.

The Effect of Eutrophication on Drinking Water

The effects of eutrophication on the environment may have deteriorate consequences of health of exposed animal and human populations through various path way’s. when fresh water, extracted from eutrophic areas, is used for the production of drinking water severe impacts can also occur during watering in eutrophic waters. eutrophication is complex processes which occurs both in fresh and marine waters, where certain types of algae disturbs the aquatic ecosystem and become a threat for animals and human health the primary cause of eutrophication is an excessive concentration of plant nutrient’s originating from agriculture of sewage treatment. The main cause of eutrophication is the large input of nutrient mixed to water body and the main effect is imbalance in the food web that results in the high levels of phytoplankton, zooplankton, algae biomass in stratified water bodies. This can lead algal blooms. The direct consequences is in an excess of oxygen consumption near bottom of the water body. eutrophication processes can be divided in to two categories depending on weather they are linked to the nutrients dispersion and phytoplankaton growth to oxygen cycle near the bottom of the water body. Various effects can be observed depending upon the severity of the eutrophication. Treatment of eutrophic water for producing drinking water. algae disturbs the aquatic ecosystems a threat for animal and human health..eutrophication concern the availability of oxygens. Some species of algae may also contain toxins but incidents where fresh water algae or the origin of cause human or animal illness. Some cynobacteria have capacity to produces toxins dangerous to human beings. A variety of symptoms depending on toxins implicated our observed such as fatigues, headache, diarrhoea, vomiting, and some throat fever skin irritations. Good practices to inform people about risks of bathing or sporting activity in normally colored or turbid waters. Allergic bathers of people walking along shore of water body affected by algae blooms. Any allergies releasing not only toxin but also allergic compounds. In some specific case; local authorities must rely on eutrophic water for producing drinking water.

Increasing air temperature relative to water temperature makes the mixed layer shallower, reducing phytoplankton biomass in a stratified lake

Abstract The depth of the mixed layer is a major determinant of nutrient and light availability for phytoplankton in stratified waterbodies. Ongoing climate change influences surface waters through meteorological forcing, which modifies the physical structure of fresh waters including the mixed layer, but effects on phytoplankton biomass are poorly known. To determine the responses of phytoplankton biomass to the depth of the mixed layer, light availability and associated meteorological forcing, we followed daily changes in weather and water column properties in a boreal lake over the first half of a summer stratification period. Phytoplankton biomass increased with the deepening of the mixed layer associated with high wind speeds and low air temperature relative to the temperature of the mixed layer (Tair−Tmix &lt; 0), whereas heatwave conditions—shallow mixed layer driven by high Tair−Tmix value and low wind speed—reduced the biomass. Improving light availability from low to moderate light conditions increased the phytoplankton biomass, while the highest light availability was associated with low phytoplankton biomass. Our study demonstrates that the climatic impact‐drivers wind speed and Tair−Tmix are major drivers of mixed layer depth, which controlled phytoplankton biomass during the early summer stratification period. Our study suggests that increasing air temperature relative to water temperature and declining wind speeds have potential to lead to reduced phytoplankton biomass due to a shallower mixed layer during the first half of the stratification period in non‐eutrophic lakes with sufficient light availability.

Taxonomic Composition of Protist Communities in the Coastal Stratified Lake Kislo-Sladkoe (Kandalaksha Bay, White Sea) Revealed by Microscopy

Lake Kislo-Sladkoe is a stratified water body partly isolated from the White Sea. Perennial meromixis in the lake irregularly alternates with mixing events. Taking into account that the protists of Arctic coastal stratified water bodies are understudied, we evaluated for the first time the vertical structure, species richness, and diversity of protists assigned to different taxonomic groups in Lake Kislo-Sladkoe using light, luminescent, and scanning electron microscopy. To test the research hypothesis that a mixing event affects the vertical stratification and species composition of protists in a stratified lake, we compared the protist communities of Lake Kislo-Sladkoe in two extremely different states: strong meromixis vs. full vertical mixing. A total of 97 morphologically distinct phototrophic, heterotrophic, and mixotrophic protists were revealed with the most diverse supertaxa SAR (59), Obazoa (9), and Excavates (14). The hidden diversity of protists (43 species) was a bit less than the active diversity (54 species). A taxonomic list and micrographs of cells for the observed protists are provided. The majority of species revealed are cosmopolitan or widespread in the northern sea waters. The vertical patterns of protist communities were absolutely different in 2018 and 2021. In July 2018, clearly distinct protist communities inhabited different layers of the lake. Bloom of cryptophyte Rhodomonas cf. baltica was detected in chemocline, whereas the maximum density of its grazers was observed in adjacent layers, mainly dinoflagellates Gymnodinium sp. and Scrippsiella trochoidea, as well as a ciliate Prorodon sp. In 2021 due to the recent mixing of lake and seawater, there were no distinct communities in the water column except the superficial 0–1 m layer of fresh water.

Intrusions of sediment laden rivers into density stratified water columns could be an unrecognized source of mixing in many lakes and coastal oceans

AbstractWhen a sediment laden river flows into a stratified water body, the water mass can either intrude as an overflow, interflow or underflow, depending upon the density contrast. Different modes of sediment driven convection occur in each case. For the case of overflows, convective sedimentation occurs beneath the plume, whereby sediment rich plumes rapidly transport fine materials to depth. If underflow of dense sediment laden waters initially occurs, then after sediment has been deposited, the light interstitial material can subsequently loft and potentially mixes the entire water column. For an interflow, both lofting and sediment driven convection can occur above and below the pycnocline. All of these different regimes can be described in terms of two dimensionless parameters: namely RS = ΔρS/ΔρC and RA = ΔρA/ΔρC, where ΔρA is the density contrast between the upper layer and the river inflow (due to just salinity or temperature differences), ΔρC is the density contrast due to sediment between river and upper‐layer, and ΔρS is the density contrast between upper and lower layers (due to just salinity or temperature differences). Laboratory experiments were used to describe the vigour of convection in terms of these dimensionless parameters, which then allows behaviour of various inflows to be predicted. In most cases the convective velocities observed were an order of magnitude faster than Stokes settling velocities. These observations are also applied to predict how a turbidity current could lead to lofting and possible overturn of the stratification of Lake Kivu, a large meromictic lake between Rwanda and the Democratic Republic of the Congo.

3D Flow Structures During Upwelling Events in Lakes of Moderate Size

AbstractUpwelling can impact water quality dynamics in stratified water bodies. Conceptual models generally classify the upwelling response to winds in lakes into either a non‐rotational (2D, closed‐basin) model or a rotational (coastal upwelling) counterpart. The non‐rotational model is broadly applied in rotationally influenced lakes. The upwelling response to winds in stratified lakes may exhibit elements of both conceptual models. Here, we present the results of simulations with an accurate three‐dimensional numerical model of a lake of moderate size (Lake Tahoe, California–Nevada, USA), and focus our analyses on addressing the influence of the Coriolis force during the upwelling setup and relaxation phases. Upwelling was observed at the upwind boundary as well as along the coast to the left of the mean wind direction due to Ekman‐driven divergence. On the downwind shoreline, where downwelling occurs, alongshore currents appeared in response to wind forcing. Following the relaxation of the wind, our simulations reveal, for the first time, the existence of powerful cyclonic alongshore currents in lakes of moderate size, which are found to be in quasi‐geostrophic balance. These currents followed the characteristics of coastal jets often observed in large lakes and the coastal ocean. Our work leads us to conclude that upwelling setup in lakes with length scales ≈20 km and Burger numbers S &lt; 1, exhibits a combination of dynamics predicted by both the non‐rotational, 2D, closed basin model, and the rotational coastal‐upwelling conceptual model.

Isotopic reconstruction of iron oxidation-reduction process based on an Archean Ocean analogue

The chemical composition and redox conditions of the Precambrian ocean are key factors for reconstructing the temporal evolution of atmospheric oxygen through time. In particular, the isotopic composition of iron are useful proxies for reconstructing paleo-ocean environments. Yet, respective processes and related signatures are poorly constrained, hindering the reconstruction of iron redox mechanisms in the Archean ocean. This study centers on Sihailongwan Lake, a stratified water body with a euxinic lower water column considered as an Archean ocean analogue. Results show that the anaerobic oxidation layer is so different from other similar lakes in which dissolved Fe oxidation is present in redoxcline layer. And the fractionation factor between ferrous Fe and iron hydroxide observed in nature water body of Sihailongwan Lake reaches to 2.6‰, which would benefit the production of the oxidations of BIF in sediment. By the spatial distribution of Fe isotope, the benthic water in autumn and the hypolimnetic anoxic water in spring has been identified as iron sulfide zone, where iron isotopic fractionation factor during iron sulfide formation is 1.16‰, accounting for partial scavenging of dissolved Fe(II) with an associated isotopic fractionation. However, pyrite in the sediment records the iron isotopic signal from the redoxcline but not in the iron sulfide or oxide zones of the water column. Above findings indicate that neither the iron isotope fractionation during partial transfer of ferrous iron to iron sulfide nor the partial oxidation of ferrous iron are recorded as pyrite in sedimentary rock. Importantly, the signal of Fe isotopic fractionation in water was archived in the suspended particulate matter and transferred into the sediment, rather than via ferrous iron directly deposited in the sediment. This study reveals that Fe isotopes from modern natural environments are useful proxies for reconstructing iron oxidation-reduction process during Earth's early history.

Trends in temperature, salinity and productivity in the Vienna Basin (Austria) during the early and middle Miocene, based on foraminiferal ecology

The Neogene Vienna Basin (VB) is a major hydrocarbon province with a long history of exploration accumulating extensive stratigraphic and structural information from numerous seismic and drilling programs. Based on the quantitative analysis of hundreds of foraminiferal samples from 52 drillings, we present the first continuous reconstruction of paleoenvironmental evolution of the VB from the early Miocene to the middle Miocene spanning 6.4 million years. Our analyses comprise reconstructions of sea surface temperature (SST), bottom water temperature (BWT), salinity, trophic levels, stress indicators, mode of life, feeding preferences and diversity indices (Fisher α, dominance and equitability) based on the transfer function for foraminifers of Hohenegger (2005, Palaeogeogr. Palaeoclimatol. Palaeoecol. 217, 115–130) and applied statistics (e.g. box plots and ternary diagrams). Bottom water temperatures indicate a cooling during the early and middle Badenian (Langhian), which seemingly contradicts the global warming of the Middle Miocene Climatic Optimum (MMCO) and a subsequent warming, which contrasts the expected trend following the cooling of the Middle Miocene Climatic Transition. Both trends are discussed as result of bathymetric evolution of the VB and intense upwelling during the early and middle Badenian. Repetitive alternations of Orbulina-rich assemblages, indicating a warm and stratified waterbody, with globigerinid-dominated assemblages, indicating upwelling and high productivity, are discussed in the light of a yet undescribed waxing and waning of upwelling intensity on a decadal to millennial scale. The applied formula for SST-calculations seems to underestimate real temperatures. Nevertheless, it reveals a distinct warming of about 3 °C from the early to the middle Miocene. In contrast to previous interpretations, we document normal marine conditions from the Ottnangian to the Sarmatian (middle Burdigalian to Serravallian) with a slight increase in salinity during the late Sarmatian.

Seasonality modulates wind-driven mixing pathways in a large lake

Turbulent mixing controls the vertical transfer of heat, gases and nutrients in stratified water bodies, shaping their response to environmental forcing. Nevertheless, due to technical limitations, the redistribution of wind-derived energy fuelling turbulence within stratified lakes has only been mapped over short (sub-annual) timescales. Here we present a year-round observational record of energy fluxes in the large Lake Geneva. Contrary to the standing view, we show that the benthic layers are the main locus for turbulent mixing only during winter. Instead, most turbulent mixing occurs in the water-column interior during the stratified summer season, when the co-occurrence of thermal stability and lighter winds weakens near-sediment currents. Since stratified conditions are becoming more prevalent –possibly reducing turbulent fluxes in deep benthic environments–, these results contribute to the ongoing efforts to anticipate the effects of climate change on freshwater quality and ecosystem services in large lakes.