In the current experimental research, we aimed to discover the interfacial behavior of gas–liquid stratified two-phase flow sub-regime by the Planar Laser-Induced Fluorescence (PLIF) method. The introduced research was conducted in a horizontal stratified flow rectangular channel, where respected flow measurements were studied using some quantitative values established visually by obtained PLIF photographs. Moreover, applied indicators in the current study focus on each of the following factors:wave dynamics, liquid film thickness, flow velocities, and frequencies of film disruption waves. Meanwhile, in order to strain the experimental efforts in the study, film thickness measurements were examined in both space and time domains. In addition, integral data supplemented against loaded spatiotemporally recovered information on interface position and two-phase velocity fields collected using planar laser generated fluorescence (PLIF).The presented study ascertains a novel relationship between dimensionless description of wave velocity, film thickness, and frequency for the liquid film. The study was performed under gas shear with inlet water Reynolds values from 88 to 437, and air Reynolds numbers ranging from 6.95 to 34.8 x103. Experimental studies concluded that different modes of wave generation with a decrement in the wave speed, film thickness, and frequency increased at a specific liquid velocity, with increasing the superficial speed. According to the findings, the experimental and theoretical values of the attendance indicators four recognized stratified flow sub-regimes presented in the results, based on both Navier-Stokes, and Kosky models. The observed flow regimes were the Ripple wave, roll wave, Jeffrey wave, and random disturbance wave. Regarding wave frequency studies, the Azzopardi and Bae models used to compare wave frequency, while the Gawas and Sarkhi models were implemented to compare wave velocity.