The influence of the mesoscale wind velocity and temperature fluctuations induced by internal gravity waves (IGWs) on infrasound propagation in the atmosphere is studied. The statistical characteristics of the fluctuations in the parameters of infrasonic signals (such as variances and temporal spectra of the fluctuations in travel time and angle of arrival, amplitude and time duration) caused by gravity wave-associated fluctuations are studied based on the nonlinear model of shaping of the 3-D spatial spectrum of the fluctuations. The nonlinear shaping mechanism for the 3-D spectrum is associated with both the non-resonance interactions between IGWs and wave breaking processes caused by the wave-induced shear or convective instabilities. The 1-D wave number spectra (vertical and horizontal) of the mesoscale fluctuations obtained from the 3-D model are compared with the observed spectra derived from the radar, lidar and airplane temperature and wind measurements in the middle atmosphere. The results of theory and numerical modeling of infrasound scattering from gravity wave-associated fluctuations are presented. The vertical profiles of the wind velocity fluctuations in the stratosphere and lower thermosphere up to the altitudes of 130 km are retrieved from the infrasound scattered from the mesoscale wind velocity and temperature fluctuations. The results of acoustic probing of the stably stratified atmospheric boundary layer using detonation source of acoustic pulses are discussed.