Development Of Strategies Research Articles

Manipulating Silver Nanoparticles with Biomolecular Corona Secreted from Vertebrates to Improve the Loading Capacity and Biocompatibility.

Silver nanoparticles (AgNPs) are widely used as nanoagents in biomedical fields, while it is still challenging to improve their loading capacity and biocompatibility in microcarrier delivering systems. Herein, the physicochemical properties of AgNPs were manipulated by forming biomolecular corona derived from bovine serum albumin (AC), and three organisms at various trophic levels: Chlorella sp. (BC1), Daphnia magna (BC2), and zebrafish (BC3). Proteins were identified by chemical composition analysis as the dominant components adsorbed on the surface of AgNPs. Proteomics indicated that AgNPs preferred to bind with low molecular weight (<50 kDa) and hydrophobic proteins with more positively charged residues. Consequently, AC and BC3 displayed stronger adsorption affinity on the surface of AgNPs than BC1 and BC2. Modifications by AC and BC3 effectively alleviated the oxidative stress and cell cycle arrest of AgNPs due to their superior antioxidative ability. However, BC3 with lower hydrophobicity enabled AgNPs to be more biocompatible than AC at subcellular level. Moreover, AC could significantly improve the loading capacity of AgNPs by Chlorella through enhancing caveolin-mediated endocytosis. Notably, owing to the adsorption of abundant Ca2+-binding proteins, BC3-AgNPs could also be internalized by microalgae via Ca2+-dependent clathrin-mediated endocytosis, which makes it a promising approach to deliver AgNPs. The results of this study would provide insights into the development of an efficient strategy to deliver AgNPs based on the microalgae carrier without altering its original properties and functionality.

Bioconjugation of Serratiopeptidase with Titanium Oxide Nanoparticles: Improving Stability and Antibacterial Properties

Antimicrobial resistance (AMR) poses a significant global health threat, necessitating the development of novel antibacterial strategies. Serratiopeptidase (SP), a metalloprotease produced by bacteria such as Serratia marcescens, has gained attention not only for its anti-inflammatory properties but also for its potential antibacterial activity. However, its protein nature makes it susceptible to pH changes and self-proteolysis, limiting its effectiveness. This study aimed to increase both the enzymatic stability and antibacterial activity of serratiopeptidase through immobilization on titanium oxide nanoparticles (TiO2-NPs), leveraging the biocompatibility and stability of these nanomaterials. Commercial TiO2-NPs were characterized using TGA/DTG, FT-IR, UV–Vis, and XRD analyses, and their biocompatibility was assessed through cytotoxicity studies. Serratiopeptidase was produced via fermentation using the C8 isolate of Serratia marcescens obtained from the intestine of Bombyx mori L., purified chromatographically, and immobilized on carboxylated nanoparticles via EDC/NHS coupling at various pH conditions. The optimal enzymatic activity was achieved by using pH 5.1 for nanoparticle activation and pH 5.5 for enzyme coupling. The resulting bioconjugate demonstrated stable proteolytic activity at 25 °C for 48 h. Immobilization was confirmed by FT-IR spectroscopy, and the Michaelis–Menten kinetics were determined. Notably, the bioconjugate exhibited two-fold greater antibacterial activity against E. coli than the free enzyme or TiO2-NPs at 1000 µg/mL. This study successfully developed a serratiopeptidase–TiO2 bioconjugate with enhanced enzymatic stability and antibacterial properties. The improved antibacterial activity of the immobilized enzyme presents a promising approach for developing new tools to combat antimicrobial resistance, with potential applications in healthcare, food safety, and environmental protection.

Neuronal Vulnerability of the Entorhinal Cortex to Tau Pathology in Alzheimer’s Disease

This review delves into the entorhinal cortex (EC) as a central player in the pathogenesis of Alzheimer’s Disease (AD), emphasizing its role in the accumulation and propagation of tau pathology. It elucidates the multifaceted functions of the EC, encompassing memory formation, spatial navigation, and olfactory processing, while exploring how disruptions in these processes contribute to cognitive decline in AD. The review discusses the intricate interplay between tau pathology and EC vulnerability, highlighting how alterations in neuronal firing patterns and synaptic function within the EC exacerbate cognitive impairments. Furthermore, it elucidates how specific neuronal subtypes within the EC exhibit differential susceptibility to tau-induced damage, contributing to disease progression. Early detection methods, such as imaging techniques and assessments of EC blood flow, are examined as potential tools for identifying tau pathology in the preclinical stages of AD. These approaches offer promise for improving diagnostic accuracy and enabling timely intervention. Therapeutic strategies targeting tau pathology within the EC are explored, including the clearance of pathological tau aggregates and the inhibition of tau aggregation processes. By understanding the molecular and cellular mechanisms underlying EC vulnerability, researchers can develop more targeted and effective interventions to slow disease progression. The review underscores the importance of reliable biomarkers to assess disease progression and therapeutic efficacy in clinical trials targeting the EC. Ultimately, it aims to contribute to the development of more effective management strategies for AD, emphasizing the translation of research findings into clinical practice to address the growing societal burden of the disease.

CD4 downregulation precedes Env expression and protects HIV-1-infected cells from ADCC mediated by non-neutralizing antibodies.

Antibody-dependent cellular cytotoxicity (ADCC) represents an effective immune response for clearing virally infected cells, making ADCC-mediating antibodies promising therapeutic candidates for HIV-1 cure strategies. Broadly neutralizing antibodies (bNAbs) target epitopes present on the native "closed" envelope glycoprotein (Env), while non-neutralizing antibodies (nnAbs) recognize epitopes exposed upon Env-CD4 interaction. Here, we provide evidence that env mRNA is predominantly expressed by productively infected cells that have already downmodulated cell-surface CD4. This indicates that CD4 downmodulation by HIV-1 precedes Env expression, making productively infected cells resistant to ADCC mediated by nnAbs but sensitive to those mediated by bnAbs. These findings offer critical insights for the development of immunotherapy-based strategies aimed at targeting and eliminating productively infected cells in people living with HIV.

Tumor cell-intrinsic Piezo2 drives radioresistance by impairing CD8+ T cell stemness maintenance.

Changes in mechanosensitive ion channels following radiation have seldom been linked to therapeutic sensitivity or specific factors involved in antitumor immunity. Here, in this study, we found that the mechanical force sensor, Piezo2, was significantly upregulated in tumor cells after radiation, and Piezo2 knockout in tumor cells enhanced tumor growth suppression by radiotherapy. Specifically, loss of Piezo2 in tumor cells induced their IL-15 expression via unleashing JAK2/STAT1/IRF-1 axis after radiation. This increase in IL-15 activates IL-15Rα on tumor-infiltrating CD8+ T cells, thereby leading to their augmented effector and stem cell-like properties, along with reduced terminal exhausted feature. Importantly, Piezo2 expression was negatively correlated with CD8 infiltration, as well as with radiosensitivity of patients with rectum adenocarcinoma receiving radiotherapy treatment. Together, our findings reveal that tumor cell-intrinsic Piezo2 induces radioresistance by dampening the IRF-1/IL-15 axis, thus leading to impaired CD8+ T cell-dependent antitumor responses, providing insights into the further development of combination strategies to treat radioresistant cancers.

Cultivating a culture of intervention: Bystander programs and the prevention of sexual violence

This systematic review examines the effectiveness of bystander intervention programs in preventing sexual violence, with a focus on the role of masculinity and cultural factors. The review synthesizes findings from six studies, employing a mixed-methods approach to analyze quantitative and qualitative data. The results highlight the significance of bystander intervention in preventing sexual violence, particularly among young people. The studies demonstrate that programs targeting men and boys, addressing traditional masculinity norms, and promoting empathy and prosocial behavior are effective in reducing sexual violence. Cultural factors, such as social norms and community engagement, also play a crucial role in shaping bystander behavior. The review concludes that bystander intervention programs have the potential to create a culture of empathy and action, but require careful consideration of cultural context and nuances. The findings have implications for the development of effective prevention strategies and highlight the need for further research in this area. By understanding the complexities of bystander behavior, we can work towards creating safer and more supportive communities for all individuals.

Renal protection of HWL-088 and ZLY032, two dual GPR40/PPARδ agonists, in adenine-induced renal fibrosis model.

This research examined the potential of novel GPR40/PPARδ dual agonists, HWL-088 and ZLY-032, to protect the kidneys in a mouse model of adenine-induced renal fibrosis. Mice were given a diet containing 0.25% adenine to develop renal fibrosis and then received different dosages of HWL-088 or ZLY-032. After being euthanized, tissue and serum samples were collected for morphological, histological, and molecular examination. Compared to the control group, mice fed adenine showed an increase in kidney-to-body weight ratio, serum creatinine, and urea levels. Hematoxylin and eosin staining revealed alleviated glomerulosclerosis, tubular dilation, and inflammatory cell infiltration in mice treated with HWL-088 or ZLY-032. Furthermore, Masson staining and immunohistochemistry demonstrated that these dual agonists protected against renal interstitial fibrosis and inflammation, corroborated by decreased expression levels of fibrosis-related proteins (TGF-β, Collα1, TIMP-1) and pro-inflammatory cytokines (TNF-α, IL-1β, IL-6). Accordingly, it can be inferred that GPR40/PPARδ dual agonists HWL-088 and ZLY-032 could yield significant renoprotective effects by inhibiting inflammation and fibrosis. Overall, these results may contribute to the development of novel therapeutic strategies for renal fibrosis.

Variant of Organizational and Functional Adaptation Structures of a Typical Situational Centre to the Emergence of Crisis Situations

Due to the growing number of crisis situations (CS) in the modern world, such as natural disasters, man-made accidents, pandemics, cyber-attacks, financial crises, wars, and other negative events, it is important for the state to develop and adapt the organizational and functional structures of information and analytical units, including situation centers (SC), to respond effectively to such cases, thereby ensuring the country’s resilience in the face of negative circumstances. The purpose of the study is to consider and analyze a possible option for adapting the organizational and functional structure of a typical SC for more efficient functioning in the context of a CS. The research methods used in the article include content analysis, analysis, induction and hypothetical method. The article highlights the organization of information interaction between state structures during full-scale conflicts, the ways of resolving the SC in such conditions and the measures taken to respond effectively in a timely manner. Particular attention is paid to approaches to information counteraction in crisis management. The authors analyze the experience of countries around the world in establishing and operating SC and developing appropriate organizational and functional structures. Based on the results of the analysis, it is concluded that today there is a tendency to establish and operate crisis centers in countries around the world. However, it has been found that no publication pays sufficient attention to approaches to adaptation (improvement) of the structures of crisis centers to the emergence of the CS. The study is of great importance, since the proposed option of adapting the organizational and functional structure of a typical crisis center to the emergence of a CS opens up new opportunities for understanding effective methods of countering a CS and promotes the development of crisis management strategies, in particular in the context of hybrid confrontation and full-scale war.

Climate change may make pine wilt disease more prevalent

Abstract Pine wilt disease is one of the most severe and devastating diseases affecting pine forests worldwide, resulting in huge economic losses in many countries. The pinewood nematode (PWN), Bursaphelenchus xylophilus, is the causal agent of pine wilt disease and is obligately vectored by pine sawyer beetles, of the genus Monochamus. For the disease to be present, the habitat must be suitable for the PWN, and include at least one vector species, and at least one host species. To predict its potential distribution, a model must consider all three components. However, no comprehensive study has examined the influence of climatic suitability on the distribution of this “biological complex”. This study addresses this gap by incorporating biotic interactions, specifically involving 13 vectors and 61 host plants, into projections based on the PWN model. We predicted the global potential distribution of pine wilt disease and compared it with the PWN model to highlight the importance of including biotic interactions in species distribution models under climate change. We found that the model revealed an overall trend of increasing suitability scores for both the PWN and pine wilt disease models under future climate scenarios. Furthermore, compared to the PWN model, the biotic model results in an apparent increase in suitability worldwide in the future as the climate will be more suitable to vector and host complexes, suggesting that pine wilt disease could potentially spread to other places via available hosts and vectors. Synthesis and applications. By incorporating biotic interactions, we projected a more accurate suitable area for pine wilt disease, offering valuable insights into regions at high risk for future invasions by the disease and its vectors. This information supports the development of management and early detection strategies in areas of high suitability, helping to mitigate potential economic and ecological losses. Additionally, this study introduces a novel approach for integrating biotic factors into species distribution models.

Epigenetic Regulation in Insect-Microbe Interactions.

Insects have evolved diverse interactions with a variety of microbes, such as pathogenic fungi, bacteria, and viruses. The immune responses of insect hosts, along with the dynamic infection process of microbes in response to the changing host environment and defenses, require rapid and fine-tuned regulation of gene expression programs. Epigenetic mechanisms, including DNA methylation, histone modifications, and noncoding RNA regulation, play important roles in regulating the expression of genes involved in insect immunity and microbial pathogenicity. This review highlights recent discoveries and insights into epigenetic regulatory mechanisms that modulate insect-microbe interactions. A deeper understanding of these regulatory mechanisms underlying insect-microbe interactions holds promise for the development of novel strategies for biological control of insect pests and mitigation of vector-borne diseases.

An engineered model of metastatic colonization of human bone marrow reveals breast cancer cell remodeling of the hematopoietic niche

Incomplete understanding of metastatic disease mechanisms continues to hinder effective treatment of cancer. Despite remarkable advancements toward the identification of druggable targets, treatment options for patients in remission following primary tumor resection remain limited. Bioengineered human tissue models of metastatic sites capable of recreating the physiologically relevant milieu of metastatic colonization may strengthen our grasp of cancer progression and contribute to the development of effective therapeutic strategies. We report the use of an engineered tissue model of human bone marrow (eBM) to identify microenvironmental cues regulating cancer cell proliferation and to investigate how triple-negative breast cancer (TNBC) cell lines influence hematopoiesis. Notably, individual stromal components of the bone marrow niche (osteoblasts, endothelial cells, and mesenchymal stem/stromal cells) were each critical for regulating tumor cell quiescence and proliferation in the three-dimensional eBM niche. We found that hematopoietic stem and progenitor cells (HSPCs) impacted TNBC cell growth and responded to cancer cell presence with a shift of HSPCs (CD34+CD38-) to downstream myeloid lineages (CD11b+CD14+). To account for tumor heterogeneity and show proof-of-concept ability for patient-specific studies, we demonstrate that patient-derived tumor organoids survive and proliferate in the eBM, resulting in distinct shifts in myelopoiesis that are similar to those observed for aggressively metastatic cell lines. We envision that this human tissue model will facilitate studies of niche-specific metastatic progression and individualized responses to treatment.

From Song to Loyalty: How the Anthem of Universitas Trunojoyo Madura Enhances Student Engagement and Strengthens Institutional Bonds

University anthems, as key elements in shaping the identity of higher education institutions, reflect the values and history of the university and enhance a sense of community. This study focuses on how the integration of traditional Madurese elements in the anthem of Universitas Trunojoyo Madura influences student identity and engagement. The knowledge gap lies in the actual influence of traditional music in enhancing student engagement and loyalty. A qualitative methodology was employed, involving in-depth interviews and lyrical content analysis of the anthem to uncover student perceptions. The primary findings suggest that the use of traditional Madurese music elements enhances student pride and engagement, strengthens institutional identity, and reinforces community bonds. The implications of these findings affirm the crucial role of arts and literature in facilitating social and communal engagement in academic settings, providing new insights for the development of educational strategies and university policies.

Human life expectancy and season of birth in Taiwan: A retrospective cohort study

Prior research has indicated a correlation between the birth season and life expectancy; however, many of these studies did not sufficiently account for comorbidities. In this comprehensive investigation, we aimed to meticulously explore the association between the birth month and life expectancy, giving due consideration to comorbidities. We used a robust dataset derived from Taiwan’s National Health Insurance Research Database (2000–2013), which allowed us to conduct a thorough examination. We divided our participants into four groups based on their season of birth: spring, summer, autumn, and winter. Propensity score matching was used to ensure an equitable distribution of demographic and clinical characteristics across the groups. Propensity scores were computed using logistic regression. Our model incorporated a broad range of demographic factors and comorbidities, providing rigorous adjustment for potential confounders. Our findings revealed a significantly increased risk of all-cause mortality among individuals born in spring, even after stringent adjustment for demographic factors and comorbidities. People born in spring demonstrated a 1.05-fold increase in the risk of all-cause mortality, with a hazard ratio of 1.05 and a 95% confidence interval of 1.01–1.09. Our study provides compelling evidence that helps understand the potential long-term impacts of a person’s birth season, which acts as a proxy for pregnancy / early-life environmental exposure, on life expectancy. These findings underscore the crucial need for additional research to illuminate the underlying biological and environmental mechanisms linking the birth season and lifespan of a person. The elucidation of these links could guide the development of innovative health promotion and disease prevention strategies that are tailored to an individual’s birth season.

ФОРСАЙТ ЯК ІНСТРУМЕНТ ВІДПОВІДАЛЬНОГО ДЕРЖАВНОГО УПРАВЛІННЯ І СТРАТЕГУВАННЯ МІЖНАРОДНОЇ ЕКОНОМІЧНОЇ ПОЛІТИКИ В УМОВАХ ЗАГОСТРЕННЯ ГЛОБАЛЬНИХ ПРОБЛЕМ РОЗВИТКУ

The purpose of the article is to study strategic long-term planning tools that allow timely identification of the latest risks, challenges and threats to international economic development that make it difficult to solve global problems that are a consequence of failures in the coordination of international economic policy. It has been determined that strategic forecasting allows politicians to increase the efficiency of government decision-making by identifying opportunities and threats that may arise in the coming years and decades, and also involves the development of possible strategies to counter them by taking into account the characteristics of the identified trends. The phenomenon of the new economy is characterized. The influence of technological development on the asymmetry of international economic development is determined. It has been established that the problems of international economic development in foresight strategizing include geopolitical rivalry, resource confrontation, energy dependence, deglobalization, disintegration processes, trade fragmentation, gaps in value and supply chains, reinstitutionalization of the system of international economic relations, recession, population aging, unemployment. Foresight forecasting tools that are not aimed at predicting a single “correct future” include: analysis of long-term trends and uncertainties; horizon scanning); scenario planning; shaping a normative future; political games; futures wheels; causal multilevel analysis. Research into long-term trends and uncertainties reveals key factors in different possible futures. The comprehensive observation carried out allows us to identify risks for the macroeconomic environment and proactively formulate a pool of measures to minimize the destructive impact of instability on socio-economic development. Improving foresight research as a critical element of strategic forecasting for solving problems of long-term development affects both theoretical aspects (conceptual approach, conceptual apparatus) and applied aspects (technologies and methods of development) of the development and use of this tool. Strategic planning allows you to develop adequate tools to achieve goals and proactively respond to the challenges of modern development, minimizing costs and compensating for the losses that arise for this reason, which lead to the loss of existing competitive advantages. The difference between forecasts and foresights is noted: forecasts are usually associated with uncontrollable events, while foresights most often deal with events and processes that are clearly controllable, therefore they assess the probability of not only one or another outcome of future events, but also the adoption of certain strategic and management decisions. The ability to distinguish normative approaches (what we want) from pragmatic approaches (what we should) is an important component of thinking in terms of alternative futures

Plants’ Impact on the Human Brain—Exploring the Neuroprotective and Neurotoxic Potential of Plants

Background: Plants have long been recognized for their potential to influence neurological health, with both neuroprotective and neurotoxic properties. This review explores the dual nature of plant-derived compounds and their impact on the human brain. Discussion: Numerous studies have highlighted the neuroprotective effects of various phytoconstituents, such as those found in Ginkgo biloba, Centella asiatica, Panax ginseng, Withania somnifera, and Curcuma longa. The neuroprotective compounds have demonstrated antioxidant, anti-inflammatory, and cognitive-enhancing properties, making them promising candidates for combating neurodegenerative diseases and improving brain function. Polyphenolic compounds, triterpenic acids, and specific phytocompounds like the ones from EGb 761 extract have shown interactions with key enzymes and receptors in the brain, leading to neuroprotective outcomes. However, this review also acknowledges the neurotoxic potential of certain plants, such as the Veratrum species, which contains steroidal alkaloids that can cause DNA damage and disrupt neurological function, or Atropa belladonna, which interfere with the normal functioning of the cholinergic system in the body, leading to a range of symptoms associated with anticholinergic toxicity. Conslusions: This review also emphasizes the need for further research to elucidate the complex mechanisms underlying the neuroprotective and neurotoxic effects of plant-derived compounds, as well as to identify novel phytoconstituents with therapeutic potential. Understanding the complex relationship between plants and the human brain is crucial for harnessing the benefits of neuroprotective compounds while mitigating the risks associated with neurotoxic substances. This review provides a comprehensive overview of the knowledge on the neurological properties of plants and highlights the importance of continued research in this field for the development of novel therapeutic strategies targeting brain health and neurological disorders.

Interactions between androgen and IGF1 axes in prostate tumorigenesis.

Androgen signalling through the androgen receptor (AR)is essential for prostate tumorigenesis. However, androgen signalling pathways also interact with other growth factor-mediated signalling pathways to regulate the prostatic cell cycle, differentiation, apoptosis and proliferation in the initiation and progression of prostate cancer. Insulin-like growth factor 1 (IGF1) is one of the most prominent growth factors in prostate tumorigenesis. Clinical and experimental evidence has demonstrated that IGF1 signalling supports both androgen-dependent and androgen-independent prostate tumorigenesis, suggesting that improved understanding of the interactions between the IGF1 and androgen axes might aid the development of new therapeutic strategies. Available data have shown a dynamic role of androgen-AR signalling in the activation of IGF1-signalling pathways by augmenting transcription of the IGF1 receptor in prostatic basal epithelial cells and by increasing IGF1 secretion through the suppression of IGF-binding protein 3 expression in prostatic stromal cells. In turn, IGF1 stimulates Wnt-β-catenin signalling in prostatic basal progenitors to promote prostatic oncogenic transformation and prostate cancer development. These findings highlight the cooperative, autocrine and paracrine interactions that underlie the oncogenic effects of androgens and IGF1 and open up new opportunities for therapeutic targeting.

Performance of the Climate Hazards Group InfraRed Precipitation with Station data (CHIRPS) Model in the Northeast Region of Brazil

Study is presented on the potential for using the tools contained in the Google Earth Engine (GEE) geospatial data processing and analysis platform in climatological studies in the Northeast Region of Brazil (NEB), in particular the validation of estimates of total annual rainfall obtained by the Climate Hazards Group InfraRed Precipitation with Station data (CHIRPS) model in seven homogeneous regions of the NEB. This data is made up of various sources of meteorological information, including information from satellites, numerical models and observations at weather stations in operational and research centers across the globe. The performance of the CHIRPS model was assessed using climatological data obtained from the National Meteorological Institute's (INMET) meteorological database, metrics and statistical tests. The results showed that the CHIRPS model has potential for use in six of the seven regions studied, with low errors and strong statistical correlation, and limitations in one of these regions. The conclusion is that this technological tool, which has a low operating cost and is free of charge, can be used to assist in climatological studies, planning and the development of strategies for mitigation, adaptation and coexistence with the dry periods observed in the sub-regions of the NEB, especially in regions that are in a situation of socio-economic vulnerability, as is the case in the semi-arid region of the NEB.

A Descriptive Study Risk Factors of Recurrent Lumbar Disk Herniation 1st April 2022 to 2nd April 2023

Purpose: The purpose of this study on recurrent lumbar disc herniation is to determine the causes, clinical findings, and incidence across different age groups above and below 60 years old, as well as gender. The aim is to evaluate the risk factors of recurrent disc herniation in the Afghani population. Methodology: To identify predictors for recurrent lumbar disc herniation (RLDH), a systematic review and meta-analysis were conducted based on existing evidence. The study aimed to provide pooled estimates that may assist various healthcare professionals, including researchers, surgeons, policymakers, caregivers, and patients, in identifying high-risk populations, preventing RLDH, and further investigating the underlying mechanisms. Findings: Risk factors for RLDH are under increasing investigation. Previous studies have examined various potential risk factors such as age, gender, body mass index (BMI), smoking, herniation type, diabetes, and herniation level. However, results from these studies have not always been consistent. For instance, while Kim et al. identified being male as a risk factor for RLDH, other studies did not find significant correlations. Inconsistencies also exist regarding smoking as a risk factor. This study aims to provide more reliable conclusions on these risk factors through a systematic approach. Unique Contribution to Theory, Practice and Policy: Understanding the risk factors associated with recurrent lumbar disc herniation is crucial for improving patient outcomes and reducing the burden on both individuals and society. By identifying and clarifying these risk factors, healthcare professionals can better tailor interventions, treatment plans, and preventive measures for individuals at high risk for RLDH. This study's findings may contribute significantly to the development of strategies aimed at mitigating the recurrence of lumbar disc herniation in the Afghani population.

Optimization of household medical waste recycling logistics routes: Considering contamination risks.

The escalating generation of household medical waste, a byproduct of industrialization and global population growth, has rendered its transportation and logistics management a critical societal concern. This study delves into the optimization of routes for vehicles within the household medical waste logistics network, a response to the imperative of managing this waste effectively. The potential for environmental and public health hazards due to improper waste disposal is acknowledged, prompting the incorporation of contamination risk, influenced by transport duration, waste volume, and wind velocity, into the analysis. To enhance the realism of the simulation, traffic congestion is integrated into the vehicle speed function, reflecting the urban roads' variability. Subsequently, a Bi-objective mixed-integer programming model is formulated to concurrently minimize total operational costs and environmental pollution risks. The complexity inherent in the optimization problem has motivated the development of the Adaptive Hybrid Artificial Fish Swarming Algorithm with Non-Dominated Sorting (AH-NSAFSA). This algorithm employs a sophisticated approach, amalgamating congestion distance and individual ranking to discern optimal solutions from the population. It incorporates a decay function to facilitate an adaptive iterative process, enhancing the algorithm's convergence properties. Furthermore, it leverages the concept of crossover-induced elimination to preserve the genetic diversity and overall robustness of the solution set. The empirical evaluation of AH-NSAFSA is conducted using a test set derived from the Solomon dataset, demonstrating the algorithm's capability to generate feasible non-dominated solutions for household medical waste recycling path planning. Comparative analysis with the Non-dominated Sorted Artificial Fish Swarm Algorithm (NSAFSA) and Non-dominated Sorted Genetic Algorithm II (NSGA-II) across metrics such as MID, SM, NOS, and CT reveals that AH-NSAFSA excels in MID, SM, and NOS, and surpasses NSAFSA in CT, albeit slightly underperforming relative to NSGA-II. The study's holistic approach to waste recycling route planning, which integrates cost-effectiveness with pollution risk and traffic congestion considerations, offers substantial support for enterprises in formulating sustainable green development strategies. AH-NSAFSA offers an eco-efficient, holistic approach to medical waste recycling, advancing sustainable management practices.

The impact of resilience on academic performance with a focus on mature learners

BackgroundResilience is an essential psychological trait that empowers individuals to adapt and thrive in the face of challenges. Although it is acknowledged that health professionals need to possess high levels of resilience, there has been limited research comparing how different groups of health students, particularly school leaver undergraduates and mature age graduate entry students, develop resilience in their coursework.MethodsThis study combines both objective (academic grades with validated survey results) and subjective data (interviews) to compare how resilience is related to academic coursework performance for two groups of pharmacy students: the mature age graduate entry (GE, N = 64) learners and school leaver undergraduate (UG, N = 208) learners. We employed a sequential explanatory mixed methods design using surveys, academic performance data and semi-structured interviews. The survey tapped constructs related to resilience (burnout, stressors and coping methods) while the interviews elicited a more nuanced understanding of individual and environmental factors.ResultsAlthough there was no statistical difference in burnout experience between the two groups, GE students exhibited more positive resilience attitudes than UG when selecting resilience statements on the survey. Both cohorts indicated in the survey that engaging in distraction activities (physical exercise, sleeping, listening to music, anything other than the stressor) was their most preferred method of relieving stress. Within UG student survey responses, those who indicated support from partners, friends and family had better academic performance, while those who did not report coping methods did worse academically. The three key environmental factors we identified that contributed to both undergraduate and graduate entry resilience were workload, feedback provision and psychosocial support.ConclusionsCurrently, there is still a need for resilience programs geared at academic success to be implemented in higher education. This study provides objective evidence of academic success coupled with exploration into the nuances of resilience amongst different student groups. It not only highlights the differing resilience development strategies and burnout coping mechanisms in emerging health professionals, but showcases the juxtaposition of two different learner groups (UG and GE students) within a discipline. The cross-cohort facilitation of learning as in the discipline-specific strategies identified can help both groups develop resilience and inform future innovations. By comparing mature-age graduate students and younger-in-age undergraduate students, we identified a wider range of strategies and more positive attitudes to burnout in mature-age students. Health and clinical educators in university health degrees, clinical placements and clinical workplaces can develop effective training materials based on findings from this study to 1) assist undergraduate younger-age health students with developing resilience and 2) further refine mature-age health students’ and practicing health professionals’ resilience in today’s fast-paced clinical workplaces.