In order to improve the precision of Strapdown Inertial Navigation System (SINS) and reduce the complexity of the traditional calibration method, a novel calibration and compensation scheme is proposed. An optimization calibration method with four-direction rotations is designed to calculate all error coefficients of Ring Laser Gyroscope (RLG) SINS in a series of constant temperatures. According to the actual working environment, the temperature errors of RLG SINS are compensated by a nonlinear interpolation compensation algorithm. The experimental results show that the inertial navigation errors of the proposed method are reduced.