Glucocorticoid is a kind of hormone synthesized from glycogen, which has many physiological functions such as improving the body’s anti fatigue, anti stress, and promoting growth. Oxidation medicine is a new medical science with dual characteristics of theory and technology, which is based on biological processes. Glucocorticoids can treat many diseases, but there are certain limitations in this process, especially in diseases that play a vital role. The purpose of this paper is to explore the change process of human glucocorticoid in the process of incremental exercise load training. This paper put forward the algorithm of incremental load training and action recognition in sports training, and analyzed the changes of human glucocorticoid (GC) in the process of incremental load training based on this research. The experimental design includes the selection of research subjects, the determination of sample size, and the setting of experimental and control groups. The load was set to start at 10 a.m., with cycling training from full effort to exhaustion. The initial load was 100 W, and it increased by 20 W every 10 min. The experimental results in this paper showed that for the changes of testosterone (T) during exercise, the salivary T secretion rate immediately after exercise was significantly different from that in the resting state at the recovery period of 30 min. For the change of GC during exercise, the secretory rate of saliva GC was 2.84 mmol/min in the resting state; the secretion rate of saliva GC was 2.46 mmol/min after 20 min of exercise and 2.15 mmol/min after 40 min of exercise; the secretion rate was 1.71 mmol/min immediately after exercise and 2.55 mmol/min after 30 min rest; there was a significant difference in the secretion rate of saliva GC for 5 times. In a word, there was no significant change in saliva T/GC value during exercise and rehabilitation under static state, which was related to strong adaptability of the body and no significant stress response.