A major part of the human diet consists of cereals and legumes all over the world. The major dietary requirement of the developing world includes seed storage proteins of grains. However, seeds are incomplete in nutrition because of their low protein content. Synthesis of seed storage proteins is partly regulated by cis-regulatory elements (CREs) and transcription factors besides post transcriptional and post-translational level. We performed an in-silico analysis to find out significant occurrences of various cis-elements/motifs in promoters of seed storage proteins (SSPs) from 26 monocots and 28 dicot plant groups. In this paper, an in-silico analysis was performed on 1 kb region of promoters of 26 monocots and 28 dicots plant of seed storage proteins (SSPs) genes to find out the occurrences of different cis elements/motifs. Further, a statistical method was used to test the significant occurrence of motifs among a large group of the CREs/motifs. The analysis reveals differential yet significant occurrence of 7 motifs in promoters of SSPs of monocots and 14 motifs in dicots plants among 225 motifs obtained through analysis using student's t-test. The study will be helpful in designing promoters or chimeric promoter by modifying the architecture of promoters with respect to significant CREs to enhanced deposition of protein in seeds and to manipulate the seed storage oil contents.