A method is described for the purification of nucleic acid from conference pear fruit. RNA was extracted from preclimacteric pears prior to storage, 60 days after storage at −1° and during the early stages of post storage ripening. In vitro translation of this RNA indicated that it was intact and showed a number of mRNA changes were occurring during both storage and subsequent ripening. Notable was the accumulation during storage of mRNAs coding for polypeptides with M r of ca 38, 44 and 45 000, and during the first 48 hr out of store a mRNA coding for a polypeptide with an M r of ca 43 000. The results show that low temperature storage and subsequent ripening of conference pears are accompanied by altered gene expression.
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