Maize Storage Research Articles

Does access to improved grain storage technology increase farmers' welfare? Experimental evidence from maize farming in Ethiopia

AbstractSeasonal price variability for cereals is two to three times higher in Africa than on the international reference market. Seasonality is even more pronounced when access to appropriate storage and opportunities for price arbitrage are limited. As smallholder farmers typically sell their production after harvest, when prices are low, this leads to lower incomes as well as higher food insecurity during the lean season, when prices are high. One solution to reduce seasonal stress is the use of improved storage technologies. Using data from a randomised controlled trial, in a major maize‐growing region of Western Ethiopia, we study the impact of hermetic bags, a technology that protects stored grain against insect pests, so that the grain can be stored longer. Despite considerable price seasonality—maize prices in the lean season are 36% higher than after harvesting—we find no evidence that hermetic bags improve welfare, except that access to these bags allowed for a marginally longer storage period of maize intended for sale by 2 weeks. But this did not translate into measurable welfare gains as we found no changes in any of our welfare outcome indicators. This ‘near‐null’ effect is due to the fact that maize storage losses in our study region are relatively lower than previous studies suggested—around 10% of the quantity stored—likely because of the widespread use of an alternative to protect maize during storage, for example a cheap but highly toxic fumigant. These findings are important for policies that seek to promote improved storage technologies in these settings.

Fungal community analysis of hot spots in bulk maize under different storage conditions

Maize is one of the main cereals used for food and livestock feed. During storage, maize is easily infected by fungi to form mildew or cause hot spots, resulting in maize quality loss and potential safety hazards. We collected maize samples from normal spots and hot spots in three warehouses with different storage conditions (i.e., normal storage, N2-controlled atmosphere storage, and phosphine fumigation treatment storage) and used high-throughput sequencing and traditional culturing methods to investigate the fungal communities inducing hot spots. The results showed that there were large differences in the fungal communities on the maize within the three storage warehouses, and the community compositions of the hot spots and normal spots were different within the same warehouse. The storage conditions were highly associated with the fungal community alpha and beta diversities. Aspergillus penicillioides, A. vitricola, and A. chevalieri were the dominant fungal species inducing hot spots. Furthermore, the grain temperature and moisture content were significantly correlated with fungal community composition in hot spots and normal spots. These data are useful for grain depot managers evaluating the potential risks of fungi-induced hot spots and for optimizing storage conditions to reduce the risks of hot spots during maize storage.

Maize storage losses, climate, and climate change in Zambia

Maize storage losses, climate, and climate change in Zambia

Novel Approach for a Controlled Delivery of Essential Oils during Long-Term Maize Storage: Clove Bud and Pennyroyal Oils Efficacy to Control Sitophilus zeamais, Reducing Grain Damage and Post-Harvest Losses

Maize grains represent a significant contribution for assuring food safety all over the globe. Sitophilus zeamais (Motschulsky) (Coleoptera: Curculionidae), also known as the maize weevil, is one of the most destructive pests in stored maize, causing qualitative and quantitative losses. To control S. zeamais populations in maize storage sites, synthetical chemical insecticides are applied. However, these are often used wastefully, have environmental implications, and can induce the development of resistant populations. In this work, the insecticidal and grain protecting efficacy of an innovative macro-capsule delivery device, loaded with essential oils from Clove bud and Pennyroyal, as well as their combined solutions, was tested against naturally S. zeamais-infested maize grains. The blend of both compounds incorporated in a controlled release device reduced losses by more than 45% over a long storage period of twenty weeks, diminishing the survivability of maize weevils by over 90%. The usage of the blend at a concentration of 370 μL⋅Lair-1 with an antioxidant showed the best results, however, by halving the concentration (185 μL⋅Lair-1), a significant control of S. zeamais populations was still achieved.

Impact of adopting maize hermetic storage technologies on smallholder farmers’ income in Gatsibo District, Rwanda

Rapid population growth has increased the global demand for food. However, some studies have revealed that more than one-third of the global food production is lost during postharvest operations along the food supply chain. Managerial and technical limitations such as a lack of proper storage facilities, poor handling practices and, associated knowledge are among the main causes of food losses particularly in Africa. Maize is among the most important staple food and cash crops across sub-Saharan Africa. In Rwanda, most of the maize produced incurs losses during storage. Hermetic storage technologies (HST) have been proven to be effective in the control of post-harvest storage losses in maize. However, their adoption in maize storage has been low with farmers continuing to use less effective technologies. Therefore, this study assessed the impact of hermetic maize storage technologies adoption, on the income of 301 randomly selected smallholder maize farmers in Gatsibo District of Rwanda, using the endogenous switching regression (ESR). The results revealed that household size, post-harvest training, access to credit, distance to input provider, and the household head's experience in maize production were the major factors influencing farmers' decision to adopt HST. The occupation of the household head, number of plots reserved for other crops, training, household size, age of the household head, and household maize self-sufficiency goal significantly influenced income for both HST adopters and non-adopters. Overall, the adoption of HST had a positive and significant impact on income from stored maize, among those who adopted it. The study recommends that the government of Rwanda and other stakeholders should support the dissemination of HST to facilitate access. Thus, increased access to institutional support services such as post-harvest training, credit access, and agricultural input supply, should be a major part of efforts aimed at promoting the effective use of hermetic maize storage technologies among smallholder maize farmers in Rwanda.

Short-Term Hermetic Storage of Wet Maize and Its Effect on Quality.

Maize is a major crop grown in many regions of the world for human consumption, starch production, and animal feed. After harvest, maize is dried to avoid spoilage caused by fungal growth. However, in the humid tropics, drying maize harvested during the rainy season poses challenges. In such instances, temporary storing maize under hermetic conditions may preserve grain quality while waiting for conditions suitable for drying. Wet maize at the moisture contents (m.c.) of 18, 21, and 24% was stored for up to 21 days in both hermetic and non-hermetic jars. The stored maize was assessed, every 7 days, for germination and related parameters, presence of visible mold, and pH. After 21 days of storage at 18, 21, and 24% m.c., maize germination decreased by 28.5, 25.2, and 95.5 percentage points, respectively, in hermetic jars; and by 28.5, 25.2, and 94.5 percentage points in non-hermetic jars (control). There was visible mold on maize stored in non-hermetic jars after 21 days regardless of m.c. Maize at 21 and 24% m.c. stored in hermetic conditions underwent lactic acid fermentation that reduced the pH. The findings suggest that maize at 18 and 21% m.c. can be stored for 14 and 7 days, respectively, under hermetic conditions without significant loss of quality. Further research is needed to thoroughly assess the application of these findings for temporarily storing and subsequently drying maize on farms and along the grain value chain.

Effects of biopesticides extracted with a homemade solvent on stored maize protection

Abstract Synthetic chemicals continue to play an important role in reducing storage losses attributable to insect pest activities. However, the adverse effects associated with some patented chemicals make synthetic pesticides less attractive and have given the drive to search for alternative methods of pest control. This study evaluated the effects of a traditional gin, akpeteshie crude extracts made of four timber species, neem (Azadirachta indica), mahogany (Khaya senegalensis), teak (Tectona grandis) and cedrela (Cedrela odorata) on the maize weevil Sitophilus zeamais on stored maize grains in the laboratory. Home-made extracts of the test tree plants at concentrations of 0.5, 1.0 and 2% were tested as grain protectants or as insect poisons. All tested extracts in their respective concentrations performed well in the reduction of live insects during maize storage as compared to a non-extract treatment. The mode of action of all the extracts was generally concentration and time-dependent. On average neem extract was the most effective followed by mahogany, teak, and cedrela in that order. Neem and mahogany extracts performed well in reducing grain damage at a concentration of 2% and at 0.5% concentration of cedrela extract respectively. All extracts reduced progeny emergence and acted both as a repellent or a toxicant. The extracts performed better as compared to the untreated control in the viability of maize seeds leading to germination, and subsequent seedling emergence. The relatively low weight loss of the stored grains treated with these crude extracts during the 90-day experimental period at a maximum concentration of 2% is predictive that they can be adopted as safe and alternative grain protectants against weevils in store. The unknown phytochemicals in these akpeteshie hardwood extracts may be responsible for the insecticidal properties against the weevils. For some concentrations of the extracts, germination was inconsistent which led to the suspicion of allelopathy.

Evaluation of selected botanicals for the management of maize weevil (Sitophilus zeamais) on maize (Zea mays L.) grain under laboratory condition in Gabilay District, Somaliland

One of the most reliable crops in the research region is maize. However, a variety of post-harvest insect pests like Sitophilus zeamais and Angoumois grain moths pose a hazard to the cultivation and storage of maize. Hence, a laboratory experiment was conducted in Gabilay District Somaliland in 2021 to assess selected botanicals effectiveness against maize weevil. The treatments of consisted of six botanicals namely neem seed, neem leaves, garlic, lantana, ginger, and pepper tree leaves at 50 g/kg of each were evaluated. Malathion 5% dust at 0.05 g/kg as standard check and the control/untreated check/were included for comparison. The Experiment was designed in a Completely Randomized Design and replicated three times, All the botanical powders were more effective than the control by causing high insect mortality, by lowering grain damage, weight loss, and emergence of F1 progeny. Neem seed and Garlic showed 100% adult mortality, which had a similar effect to Malathion 5% dust. Neem seeds produced the lowest F1 offspring, followed by garlic. Neem seeds and garlic had the least amount of seed damage among the botanicals (0%) and (4%), respectively. The control/untreated check/resulted in the most seed damage (45%). Similarly, the highest weight loss among the botanicals was recorded on the control check, Ginger and Pepper tree 11%, 5%, and 4% respectively. The ability of seeds to germinate was unaffected by the botanicals. A conclusion, neem seed and garlic leaves are the most effective treatment against maize weevil.

Storage conditions and postharvest practices lead to aflatoxin contamination in maize in two counties (Makueni and Baringo) in Kenya

Abstract Aflatoxins are known to cause devastating acute and chronic effects in humans and animals. The objective of the study was to determine the influence of postharvest practices and storage conditions on aflatoxin contamination in maize in two counties. Aflatoxin levels in 144 maize samples from different maize storage conditions were determined. While sampling, a structured questionnaire was also administered to evaluate farmer’s postharvest practices. Makueni County had the highest percentage of aflatoxin positive samples with up to 174 ppb attributed to the long storage under unfavourable conditions. On the other hand, Baringo County had lower positivity associated with the harvesting season at the time of sample collection. The type of storage condition had a significant effect on the extent of contamination and accounted for 11% of the variation (R 2 = 0.11). Gunny bags were the most common type of storage condition and had the highest level of contamination in both the counties. Metallic bins had the lowest level of contamination. Aflatoxin G1 and G2 were predominant in samples from Baringo County, while aflatoxin B1 and B2 were predominant in samples from Makueni County. The study concluded that the type of storage condition significantly contributes to the aflatoxin contamination in the stored maize. Proper drying of maize to the recommended moisture content and subsequent storage in hermetic structures will reduce the cases of aflatoxin contamination.

TINGKAT PERTUMBUHAN ASPERGILLUS FLAVUS SP DAN PEMBENTUKAN AFLATOKSIN PADA BERBAGAI METODE PENYIMPANAN DENGAN KADAR AIR BIJI JAGUNG PAKAN

The objectives of this study are 1) To determine the best storage method for maize forgrowth Aspergillus Flavus sp and aflatoxin formation, 2) To determine the best water content regulation during storage of maize which can suppressgrowth Aspergillus Flavus sp and aflatoxin formation. This research consisted of two stages, namely corn sampling and main research. In the main research, analysis of moisture content with different storage methods, growth analysis of Aspergillus Flavus sp and levels of aflatoxin using the ELISA method was carried out. The statistical analysis method used was a completely randomized design with two factors. From the results of the Water Content analysis, the highest increase in water content for all treatments was seen in the storage treatment using HDPE plastic at 30% moisture content, namely the initial water content of 30 increased to 35.55%, with an increase of 5.55%. Whereas the lowest increase in water content for all treatments was seen in the storage treatment with spread at 15% moisture content, namely with an initial water content of 15% to 15.64% with an increase of 0.64%. Theincrease in the growth of Aspergillus flavus sp highestfor all treatments was seen in the storage treatment by packaging using HDPE plastic at 30% moisture content, namely thegrowth rate Aspergillus flavus sp initialof 1.97 x 103 increased to 88.93 x103, while the increase in the growth of Aspergillus flavus sp. The lowest for all treatments was seen in the storage treatment with spread at 15% moisture content, namely the growth of Aspergillus flavus sp early 1.54 x 103 to 33.34x103. The highest aflatoxin test was at the water content level of 30% with the storage method packed using HDPE plastic, while the lowest aflatoxin test result was at the 15% moisture level with the storage method spread out

Knowledge regarding the ill effects of Grain Dust Exposure on Respiratory System and its Prevention among Flour Mill workers in Bangalore Rural

Grain dust is the dust produced during harvesting, drying, handling, storage or processing of wheat, oats, rice, barley or maize. Grain dust is composed of a large number of materials including various types of grain and their disintegration products, silica, fungi, insects and mites. The study aims to assess the knowledge and determine the relationship regarding the ill effects of grain dust exposure on respiratory system among flour mill workers. The research approach for this study is explorative and descriptive survey. The researcher planned to explore the knowledge regarding the ill effects of grain dust exposure on respiratory system and its prevention. The structured interview schedules were tried out with flour mill workers in selected flour mill at Bangalore rural. Split half method was done and co- efficient correlation was found (r= 0.82) which indicates high degree of reliability. Finding of the study showed, knowledge score of the flour mill workers 82% of them were having below average knowledge and 10% of them were having average knowledge and 8% of them only having good knowledge regarding the ill effects of grain dust exposure. The study concluded that the knowledge of mill workers is a basic education programme in community area such as occupational health. The primary task of nursing education is to improve the knowledge and practices to prevent the ill effects among occupational development among the community.

Cost-benefit analysis of Purdue Improved Crop Storage bags for maize storage among smallholder farmers in northwest Ethiopia

In Ethiopia, post-harvest losses caused by insects are a major challenge in crop production systems. Dried maize is particularly susceptible to insects during storage. Storage loss affects the livelihoods of small-scale farmers leading to food insecurity and loss of income. Therefore, the objective of this study was to assess the cost-benefit analysis of Purdue Improved Crop Storage (PICS) bags on maize storage in Northwest Ethiopia. Cross-sectional data was collected from 392 randomly selected households from both users and non-users of PICS bags, using stratified sampling technique. A structured questionnaire, key informant interviews, focus group discussions, individual in-depth interviews, and field observations were used to gather the data. A cost-benefit analysis was computed to evaluate the viability of PICS bags for maize storage. The binary logistic regression model was used to identify factors that affect the use of PICS bags. Descriptive statistics (percentage, mean, and standard deviation) and inferential statistics (t-test and chi-square test) were employed to analyze the data. The benefit-to-cost ratios (BCRs) of insecticide with both ordinary and PICS bags were greater than one, but PICS bags resulted in more than two-fold higher values as compared to insecticide with ordinary bags. The net present value (NPV) at 15% discount rate in 2018 was 20.73 USD and 25.35 USD per 100 kilograms of stored maize when insecticide was applied to ordinary and PICS bags, respectively. Sensitivity analysis with a 10% cost increment and up to 50% price discount showed that both technologies would still be viable for maize storage. However, PICS bags had higher NPV and BCR; making the technology more viable than insecticide with ordinary bags. The results of binary logit model indicated that educational level, gender, awareness, training, accessibility of the technology, perception of the technology, involvement in leadership activities in the community, and total income of the household positively influenced farmers’ decisions to use PICS bags, whereas price negatively affected the use of the PICS bags. PICS bags had clear economic advantage over insecticide with ordinary bags for maize storage in Northwest Ethiopia. Efforts should be made to disseminate and improve access to PICS bags for strengthening food security and increasing incomes of maize farmers in Northwest Ethiopia. Key words: Hermetic bag, smallholder farmers, profitability, post-harvest management, food security

Release kinetics of [lidocainium][ibuprofenate] as Active Pharmaceutical Ingredient-Ionic Liquid from a plasticized zein matrix in simulated digestion

An in vitro approach is proposed to study the release of an Active Pharmaceutical Ingredient-Ionic Liquid (API-IL) from a natural biopolymer matrix based on zein, a maize storage protein. Zein can be processed in the molten state with 20 w% [Lidocainium][Ibuprofenate] added as API-IL also acting as plasticizer and potentially co-plasticized by glycerol. The thermal stability of the matrix is checked, as well as the in vivo biological activity of the API-IL confirming anesthetic and anti-inflammatory activities. Model tablets are thermomolded at 130 °C (∅20 mm, 0.2 mm thick) and submitted to simulated digestion based on the INFOGEST static protocol of gastrointestinal food digestion at 37 °C (2 h under gastric conditions followed by 2 h under intestinal ones). The release of the API-IL is evaluated by HPLC-UV to dissociate lidocainium, that shows a progressive release (35 % after 2 h and 60 % after 4 h digestion), from ibuprofenate, that is mainly released under intestinal conditions due to low solubility in acidic conditions. The monitoring of the tablets reveals release mechanisms based on diffusion without noticeable erosion of the matrix. These results demonstrate the interest of this thermoplastic material to provide a relevant drug delivery system.

Effect of socio-cultural factors on gendered decision-making in the adoption of improved maize storage technologies

Effect of socio-cultural factors on gendered decision-making in the adoption of improved maize storage technologies

Factors that influence smallholder farmers' decisions to employ hermetic bag technology for maize grain storage in Kilosa District, Tanzania

The huge maize grain losses caused by a lack of improved storage methods have a negative impact on the livelihoods of smallholder farmers and national food security. Despite the effectiveness of hermetic bags against pest insects and fungal growth; and aflatoxins accumulation, the technology is not widely used by smallholder farmers. The survey study was conducted to evaluate the factors that influence the adoption of hermetic bag technology among smallholder farmers in Kilosa district. A total of 180 respondents were purposively selected based on their ability to store maize produce for six months. The data was collected using a smartphone android and responses recorded by open data kit (ODK). The data was analyzed using descriptive statistics and a binary logistic regression model in SPSS software. The descriptive results indicated that overall existing maize storage options among smallholder farmers included interwoven polypropylene bags 43.5%, hermetic bags 17.8% and synthetic chemicals and metal silos account for 38% of respondents. The model results indicated that price, access, awareness, farmer’s experience and training factors had a positive and significant impact on the use of hermetic bags. The price variable significantly and negatively influenced the use of hermetic bags at a P<0.01 level of significance. Other independent variables such as awareness, training, access and farmers experience were positively significant at P<0.05 level of significant. The government and other actors could promote and disseminate hermetic storage bag technology as a sustainable approach of lowering grain storage losses while taking identified factors into account.

Assessment of the Purdue Improved Crop Storage (PICS) bag for maize storage in Ghana

Despite the recent introduction of improved grain storage methods and technologies, many smallholder maize farmers in Ghana still use traditional storage practices and structures for storing their maize grains after harvest. This practice contributes to the high post-harvest losses in maize grain at the smallholder level largely due to insect pest infestation. Hermetic bag storage is a proven technology effective in reducing grain damage and losses from insect pests. In this study, the efficacy of the Purdue Improved Crop Storage (PICS) bag was compared with a polypropylene (PP) bag stored with maize treated with Betallic Super EC chemical and PP bag stored with maize without Betallic treatment (Control) during a 6-month storage period. Data on grain moisture content (MC), number of live insects, insect damaged kernels (IDK) and maize weight loss were collected monthly for analysis. Grain viability and nutrient analysis were also conducted before and after storage. The results showed initial grain moisture content of 11.4% was not significantly affected in the PICS bags but increased by 1-2% in the PP bags. Purdue Improved Crop Storage (PICS) bags and Betallic treatment significantly reduced insect damage grains with mean weight loss of < 5% and 6.35 % respectively compared to the control (PP bag without Betallic) treatment with mean weight loss of 21 % over the 6-month storage period. Germination rate of sampled seeds after storage in the PICS bags (75 %) was not significantly different to the initial germination rate (78 %) compared to the control (PP bag without Betallic) treatment of 56 %. Overall, maize grains stored in the PICS bags showed no signs of deterioration as compared to grains in the PP bags. The PICS bags were superior to the PP bags in terms of other grain quality metrics assessed and the nutrient characterization such as protein and carbohydrate which had no significant difference (p<0.05) between the initial and the three treatments. The results showed that PICS bags can be effective in protecting maize grains during storage. Smallholder farmers are therefore encouraged to consider this technology especially for controlling insect pests of stored maize. Key words: Insect pests, Polypropylene, Smallholder farmers, Purdue Improved Crop Storage bag

Efficacy of locally available materials and Purdue improved crop storage bag as hermetic storage of maize

Maize (Zea mays) is an important staple food crop for consumption and income. However, weevils continue to endanger household food security. A study was carried out in Bahir Dar University, Ethiopia, in 2019/2020, to develop more accessible and effective hermetic storage systems. Purdue Improved Crop Storage System (PICS) is good, but it is neither accessible nor affordable in Ethiopia. Therefore, other materials were tested in a completely randomised design replicated three times. After 3 months, number of alive and dead weevils, number of seeds with holes/grain damage and weight loss were assessed and analyzed using one-way ANOVA. Malathion treated grains, PICS, polyethylene bags and jerry cans had low weevil infestation, more dead weevils and less grain damage. Mean number of weevils alive per sample varied from 0 on Malathion to 200 on woven polypropylene bags. PICS bags were effective but could be replaced by polyethylene bags and jerry cans.

Price risk and small farmer maize storage in Sub‐Saharan Africa: New insights into a long‐standing puzzle

AbstractMaize prices fluctuate across seasons in rural markets in many low income countries, and agricultural households struggle to sell their grain at an optimal time. We provide new insight into a persistent puzzle: small farmers in low‐income countries tend to sell their maize at harvest, when prices are low, rather than storing and waiting until prices increase later in the year. Our descriptive analysis uses 20 years of data from 1038 retail markets in 30 African countries to demonstrate that the lean season price (the “high price” season) fails to rise above the harvest season price (the “low price” season) 16.3% of the time on average in countries with a single maize season and between 15.9% and 24.9% on average in countries with two maize seasons. On the basis of that stylized fact, we propose that aversion to these negative returns may contribute to farmers' decisions to opt out of storage at harvest. Using a smaller dataset of 425 retail markets in 10 countries with a single maize season, we show that even moderately risk averse farmers would opt out of storage in 15.3% of market years. Farmers cannot always predict returns to storage and output price risk may dissuade farmers from storing maize for the purpose of future sales, despite credit access. Our results indicate that other mechanisms, such as forward contracts or multiyear credit, may be necessary to mitigate downside price risk.

Storage losses, market development and household maize-selling decisions in China

PurposeThe purpose of this paper is to analyse the impacts of storage losses and market development on the maize-selling behaviours of rural households in China.Design/methodology/approachBased on the survey data of 543 households from nine major maize production provinces in China, the authors introduce storage losses to a household's maize-selling decision-making model and use fractional logit model and ordered probit model to empirically analyse the impact of maize storage losses and market development on household maize-selling decisions in China. To overcome potential endogeneity problems, the authors select the weather at drying (whether bad weather occurs during the drying process) and harvest loss as instrumental variables and re-estimate the model.FindingsThe results show that increased storage losses prompt farmers to increase the proportion of maize sold within three months after harvest and sell maize in advance. Meanwhile, the degree of market development has a significant impact on farmers' maize-selling decisions. Other factors, such as the maize output, non-agricultural employment and awareness of loss control, also affect farmers' maize-selling behaviours.Research limitations/implicationsThe government should promote advanced storage facilities, reduce household storage losses, decrease the phenomenon of centralised sales after harvest and help farmers freely choose the suitable time for sales. The government also needs to strengthen market information releases and publicity, reduce transaction costs and help farmers make reasonable sales decisions.Originality/valueThe authors introduce storage losses as a separate variable in a farmer's grain-selling decision model to empirically analyse the impact of storage losses on farmers' grain-selling behaviours. Moreover, the authors analyse the impact of market development on household grain-selling behaviours in China. These findings can help avoid oversupply in the market during the harvest season and alleviate the pressure on the market from the supply and demand imbalance. These results are also beneficial for farmers waiting for a higher price and increasing their income.

Pre-harvest strategy for reducing aflatoxin accumulation during storage of maize in Argentina

Maize (Zea mays L.) is an important crop in Argentina. Aspergillus flavus may infect this crop at growing stage and the harvested kernels can be contaminated with aflatoxins (AFs), whose levels may increase during storage. In Argentina, silo bags, a hermetic type of storage system, are widely used. Biocontrol based on competitive exclusion by atoxigenic A. flavus strains is a useful tool for AFs management at pre-harvest stage. The aim of the present study was to evaluate the effect of pre-harvest biocontrol treatments on aflatoxin B1 (AFB1) accumulation in maize stored in silo bags during 3 and 6 months. Three bioformulations based on A. flavus AFCHG2 and ARG5/30 strains were applied during field trials as single and mixed inocula. Harvested kernels were stored in non-hermetic and hermetic silo bags. At initial time (t0), 3 and 6 months (t3 and t6) the following parameters were evaluated: percentage of damaged kernels, moisture content, water activity, Aspergillus section Flavi incidence, relative humidity, O2 and CO2 levels into the silo bags, and AFB1 levels. The biocontrol strains included in the 3 bioformulations were able to infect maize kernels during the field trial and displaced native toxigenic isolates. At t0 control plots showed 10.9 ± 0.4 μg/kg of AFB1 while no AFs were detected in all the treatments. Along the storage assay AFB1 levels varied from not detected (<1 μg/kg) to 20.1 ± 0.8 μg/kg. Hermetic bags were better than non-hermetic bags in preventing AFB1 accumulation. Both single and mixed inocula were effective to control AFB1 accumulation in maize kernels during 3 and 6 months. AFB1 was not detected in kernels from the treatment at field stage with AFCHG2 + ARG5/30 after 6 months of storage into hermetic bags. The application of the biocontrol agents at field stage is an appropriate tool to reduce AFB1 accumulation under storage in hermetic silo bags. This is the first report on biocontrol strategy based on native atoxigenic strains applied at pre-harvest stage to reduce AFB1 accumulation during storage in Argentina.