Length Of Storage Research Articles

Moesin functionality in hypothermic liver preservation injury

The objective of this study was to determine how expression and functionality of the cytoskeletal linker protein moesin is involved in hepatic hypothermic preservation injury. Mouse livers were cold stored in University of Wisconsin (UW) solution and reperfused on an isolated perfused liver (IPL) device for one hour. Human hepatocytes (HepG2) and human or murine sinusoidal endothelial cells (SECs) were cold stored and rewarmed to induce hypothermic preservation injury. The cells were transfected with: wild type moesin, an siRNA duplex specific for moesin, and the moesin mutants T558D and T558A. Tissue and cell moesin expression and its binding to actin were determined by Western blot. Liver IPL functional outcomes deteriorated proportional to the length of cold storage, which correlated with moesin disassociation from the actin cytoskeleton. Cell viability (LDH and WST-8) in the cell models progressively declined with increasing preservation time, which also correlated with moesin disassociation. Transfection of a moesin containing plasmid or an siRNA duplex specific for moesin into HepG2 cells resulted in increased and decreased moesin expression, respectively. Overexpression of moesin protected while moesin knock-down potentiated preservation injury in the HepG2 cell model. Hepatocytes expressing the T558A (inactive) and T558D (active) moesin binding mutants demonstrated significantly more and less preservation injury, respectively. Cold storage time dependently caused hepatocyte detachment from the matrix and cell death, which was prevented by the T558D active moesin mutation. In conclusion, moesin is causally involved in hypothermic liver cell preservation injury through control of its active binding molecular functionality.

Biocontrol activity of an alkaline serine protease from Aureobasidium pullulans expressed in Pichia pastoris against four postharvest pathogens on apple

The yeast-like fungus Aureobasidium pullulans PL5 is a microbial antagonist against postharvest pathogens of fruits. The strain is able to produce hydrolases, including glucanases, chitinases and proteases. The alkaline serine protease gene ALP5 from A. pullulans was cloned, inserted into the vector pPIC9 to construct pPIC9/ALP5, and then expressed in Pichia pastoris strain KM71. ALP5 had a molecular mass of 42.9kDa after 5days growth with 1% methanol induction at 28°C. The recombinant protease expressed in P. pastoris showed its highest activity under alkaline conditions (at pH10) and a temperature of 50°C. The antifungal activity of the recombinant protease was investigated against Penicillium expansum, Botrytis cinerea, Monilinia fructicola and Alternaria alternata in vitro and on apple. The recombinant protease reduced significantly the spore germination and the germ tube length of the tested pathogens in PDB medium. The highest level of protease efficacy was observed against M. fructicola and B. cinerea, whereas a lower efficacy was observed against P. expansum and A. alternata indicating a possible effect of the pathogen cell wall composition on the proteolytic activity of the recombinant protease. The presence of protease was able to cause the swelling of the hyphae of B. cinerea, under an optical microscope. The recombinant protease expressed in P. pastoris was more active against the pathogens in vitro than the same enzyme expressed in E. coli in previous studies. The efficacy of ALP5 was also evaluated against the pathogens in vivo on cv Golden Delicious apples. The protease was more efficient in controlling M. fructicola, B. cinerea and P. expansum than A. alternata. However, the extent of the activity was dependent on the enzyme concentration and the length of fruit storage. This study demonstrated the capacity of the alkaline serine protease to keep its enzymatic activity for some days in the unfavorable environment of the fruit wounds. The alkaline serine protease could be developed as a postharvest treatment with antimicrobial activity for fruit undergoing a short storage period.

The Market for Blood.

Donating blood, “the gift of life,” is among the noblest activities and it is performed worldwide nearly 100 million times annually. The economic perspective presented here shows how the gift of life, albeit noble and often motivated by altruism, is heavily influenced by standard economic forces including supply and demand, economies of scale, and moral hazard. These forces, shaped by technological advances, have driven the evolution of blood donation markets from thin one-to-one “marriage markets,” in which each recipient needed a personal blood donor, to thick, impersonalized, diffuse markets. Today, imbalances between aggregate supply and demand are a major challenge in blood markets, including excess supply after disasters and insufficient supply at other times. These imbalances are not unexpected given that the blood market operates without market prices and with limited storage length (about six weeks) for whole blood. Yet shifting to a system of paying blood donors seems a practical impossibility given attitudes toward paying blood donors and concerns that a paid system could compromise blood safety. Nonetheless, we believe that an economic perspective offers promising directions to increase supply and improve the supply and demand balance even in the presence of volunteer supply and with the absence of market prices.

Chemical weathering under the Greenland Ice Sheet

To contrast continental and alpine subglacial weathering regimes and thereby assess the role of large ice masses in chemical weathering, borehole and outlet water samples were collected from multiple locations on a major, land-terminating outlet of the Greenland Ice Sheet. Boreholes, reaching ice depths to 824 m, were drilled to the bed with hot-water methods in four areas of the ice sheet ablation zone along a 45 km transect extending inland from the outlet terminus. The bulk chemical composition of these samples shows substantially less influence of sulfides and carbonates than found in alpine glaciers, suggesting that the sediment under this region of the ice sheet has become depleted of accessory minerals. The waters show wide variability in chemical composition over both large and small temporal-spatial scales, suggesting large ranges in length of subglacial water storage and in rates of abrasion and comminution of subglacial earth materials. The dissolved solids concentrations found in the Greenland Ice Sheet are comparable to and in some cases exceed those of alpine glaciers, suggesting that large ice masses are capable of generating substantial dissolved loads through silicate weathering mechanisms.

Aerobic stability of maize silage stored under plastic films with different oxygen permeability

The most important factor that can influence silage quality is the degree of anaerobiosis maintained during conservation. The quality of the plastic film is a key factor, since the permeability of polyethylene to oxygen is too high for silage conservation. The aim of this work was to assess the effects of the interaction between three plastic films with different degrees of oxygen permeability and two different maize silage conservation times on fermentation, microbial quality and aerobic stability. The conservation time affected the pH, lactic and acetic acids, 1,2-propanediol and lactic/acetic acid ratio, with higher pH, lower lactic acid concentration and lactic/acetic acid ratio but higher acetic acid and 1,2-propanediol concentrations being found in silages conserved for 110 days versus silages conserved for 55 days. The plastic film affected the pH, lactic and acetic acids and lactic/acetic acid ratio. The yeast count was lower and aerobic stability higher for silage conserved under the oxygen barrier film for both conservation periods. The positive interaction between length of storage and the increased anaerobiosis of silage during conservation provided by the oxygen barrier compared with polyethylene film helped reduce the yeast count and increase the aerobic stability of maize silage, even when less acetic acid was produced during ensiling.

Effects of storage temperature and length of the storage period on hatchability and performance of red-legged partridge (Alectoris rufa) eggs

The aim of the present study was to investigate, in red-legged partridge (Alectoris rufa) eggs, the effects of 7- and 42-d storage periods with different storage temperatures (15, 12, and 9°C) on egg weight loss, hatchability, chick weight at hatch, incubation length, and development stage at embryonic mortality. A total of 420 red-legged partridge eggs were arranged in a 2 × 3 factorial design with 2 levels of storage length and 3 levels of storage temperature, resulting in 6 treatments consisting of 10 replications of 7 eggs each. We found that the storage length significantly reduced hatchability of the fertile eggs (P = 0.001), increasing late embryonic mortality (P = 0.001). Storage temperature did not influence on the embryonic mortality at any stage (P > 0.05). Egg weight loss during storage increased with the storage length (P < 0.001), storage temperature (P < 0.001), and their interaction (P < 0.001). Incubation length increased with the storage length (P < 0.001); however, it was not influenced by the storage temperature (P > 0.05). Nevertheless, incubation period decreased with the storage temperature for 7-d storage, and increased with the storage temperature for 42-d storage (P = 0.005). It can be concluded that in this study red-legged partridge eggs stored well with little deterioration up to 42 d at 9 and 12°C and 80% RH, in contrast to the lesser durability of eggs described in the literature for other poultry species. In case of 7-d storage periods, hatchability of A. rufa fertile eggs is higher when they are stored at 15°C. These findings are useful to address specific demands of game farms that require fertile eggs for hatching whose shelf-life should be long enough to maintain hatchability until further incubation. And, due to the marked reproductive seasonality of red-legged partridge, long-term storage of hatching eggs could permit the distribution of batches of chicks throughout the year.

The length of time that flexible endoscopes which have undergone reprocessing with high-level disinfection can safely be stored before use: a systematic review protocol

Review question/objective How long (in days) do flexible endoscopes that have undergone reprocessing with high-level disinfection (HLD) maintain the same level of disinfection and remain safe to use when stored as recommended by the manufacturer? The objective of this review is to systematically review the best available evidence related to the safe hang time or storage time (in days) of flexible endoscopes that have undergone reprocessing with high-level disinfection in order to determine when they can be safely used without posing any risk of contamination from pathogens. Inclusion criteria Types of participants This systematic review does not aim to identify and include studies that have involved human participants, but rather that have included high-level disinfected endoscopes that are not immediately used. The review’s approach with respect to the type of endoscope is inclusive and with the exception of one kind, studies that have focused on use, reprocessing and storage hang time of any type of endoscope (e.g. gastroscope, bronchoscope, jejunoscope, laryngoscope, colonoscope, etc.) are to be considered for inclusion. The exception endoscope, that will not be considered, is the natural orifice translumenal endoscopy (NOTES). This is because NOTES is an emerging technology where a surgical procedure is performed to gain access to an organ (e.g. kidney, gall bladder) through a natural orifice.24 To be considered for inclusion, the endoscopes must have been cleaned using the recommended reprocessing steps described previously. In addition, the studies must have included reports of negative or positive culture of microorganisms and the specific length of time that cultures were obtained following reprocessing. Phenomena of interest The phenomena of interest of the proposed review is the length of time that endoscopes which have been properly reprocessed and stored can remain in a storage cabinet or closet before reuse without presenting a contamination risk. Types of outcomes This review will consider studies that include the following outcome measures: length of endoscope storage and measure of microorganism growth (e.g. cultures). The studies must also include the type of endoscopes used, as well as the method used to contaminate the endoscopes and specific reprocessing steps (e.g. type of rinse water, specific high level disinfectant, manual or automated reprocessing, method of drying before storage).

Effects of storage length on the hatchability of ostrich (Struthio camelus) eggs

Aim: This study was carried out in order to determine the effects of storage length on the hatchability of ostrich eggs obtained from private ostrich farms. Materials and Methods: The eggs, which were collected from ostriches in one season, lasting from March to September, were numbered, weighed, recorded with laying date and stored for 1-15 days. On the 14 th day of incubation, infertile eggs were determined and removed from incubation. On the 38 th day of incubation, eggs were weighed for the second time, and following weight losses measurement, the eggs were transferred to the hatching machine. The hatched chicks were weighed and hatchability of fertile egg, hatchability of egg set and chick weight results were determined. Results: Average incubation period was 43.58 days. In terms of incubation period, differences between groups that were classified according to storage periods (short, medium and long) were significant (P<0.05). Average egg weight loss of during incubation was 13.70%. Mean hatchabilities of egg set and fertile egg were 57.90% and 75.00%, respectively and differences between the groups of storage period were statistically significant (P<0.05). Conclusions: The storage period more than ten days has the potential to negatively affect the incubation results of ostrich eggs. So, it could be recommended that for storing ostrich eggs, 10 days of storage shouldn't be exceeded.

PP-029 Development of ready-to-use adrenaline syringes for emergency use

BackgroundAdrenaline (epinephrine) is commonly used in cardiac arrest, bronchospasm and anaphylaxis. To make the process safer and reduce the preparation time in wards in case of emergency, the availability of...

English

Shelf-life of five horticultural produce were studied. These include three leafy vegetables: Telfairia occidentalis, Celosia argentea and Amaranthus cruentus and two fruit vegetables: Lycopersicum esculentum and Abelmuschus esculentus. The layout plan of the experiment was a 3x2 factorial in a completely randomized design and each treatment replicated three times. The two factors examined were moisture barrier at three levels namely: thick lining, thin lining and non-lining. The other factor included initial moisture content of the produce, namely, turgid and partially wilted. Partial wilting of the produce was achieved by exposing freshly harvested materials at ambient temperature to dry for 45 min. During this period, about 20% of the moisture content was lost. Eighteen (18) vegetable baskets which work on the principle of evaporative cooling system were used. Each type of produce was stored at a time inside the vegetable basket. Some quantity of each produce were kept on the laboratory benches to serve as controls. Data recorded includes length of storage of produce, severity of disease infection, visual quality, disease incidence, ambient temperature and relative humidity of the storage baskets. The result indicates that there was a significantly higher relative humidity (P< 0.05) in the lined baskets than in the non-lined baskets. Consequently, the shelf-life of produce in lined basket was prolonged. Turgid produce had better quality retention and stored much longer than partially wilted produce. Generally, the evaporative coolant baskets provided an average temperature of 3°C lower than the ambient condition. The shelf-life of leaf vegetables T. occidentalis, C. argentea and A. cruentus was extended appreciably for 78 days compared to the controls.

Comparative polyphenolic antioxidant profile and quality of traditional apple cultivars as affected by cold storage

Summary‘Kathista’ and ‘Lortiko’ are regarded as traditional and highly appreciated apple cultivars in Cyprus, yet their postharvest behaviour and phytochemical content are largely unknown. Such fruits were examined for their qualitative traits, phenolic composition and antioxidant capacity after harvest or short, intermediate or extended cold storage and additional ripening at room temperature (RT), using ‘Gala’ as reference cultivar. Total phenolic content was dependent on cultivar and length of cold storage, while hydroxycinnamic acids were always at higher levels compared to total flavonols for all the examined cultivars. No clear trends regarding the influence of cold storage or RT maintenance on fruit antioxidant properties were evident. Overall, quality attributes and polyphenolic content of the traditional apple cultivars were comparable with ‘Gala’ at harvest; thus, they can be considered a good source of nutraceuticals as summer apple cultivars. However, extended cold storage deteriorates fruit behaviour and antioxidant capacity, particularly in ‘Lortiko’ fruit.

Evaluation of mortadella formulated with carbon monoxide-treated porcine blood

The proximate composition and color of mortadellas containing carbon monoxide-treated (COTB), untreated (UNTB), or CO-treated dried blood (CODB) were compared to that of control mortadella. Blood addition did not affect (P>0.05) the proximate composition and TBARS. The mortadella containing 10% UNTB were brown and those containing COTB or CODB were red. Residual nitrite level, L*, a*, b* and c* values of the mortadella decreased (P<0.05) with an increase in the amount of blood; TBARs did not vary (P>0.05). Increasing the amount of blood increased (P<0.05) the hue angle (h*) and browning index (BI) of the mortadella containing UNTB. Increasing blood addition decreased (P<0.05) h* and did not affect (P>0.05) BI. Increasing storage length decreased (P<0.05) residual nitrite, affected BI and color coordinates and did not affect TBARS (P>0.05). Addition of CO-treated blood allows the production of better-colored sausages having lower residual nitrite levels.

Biobanking Practice: RNA Storage at Low Concentration Affects Integrity

A critical issue in defining protocols for biobanking practices is the preservation of total RNA for assessing the whole transcriptome and ensuring that it can be utilized in clinically oriented studies. Storage conditions, such as temperature and the length of time that tissues and purified RNA stay frozen, may directly impact RNA preservation. In this study, we evaluated a) the quality of RNA (as measured by RNA Integrity Number) purified from head and neck tumor tissues stored at -140°C for distinct time intervals of up to 7 years, and b) the quality of their respective RNAs stored for 4 years at -80°C when diluted at either 250 ng/μL or 25 ng/μL, with repeated freezing and thawing. Additionally, we generated a profile of the RNA collection of human tumors from different body sites stored at the AC Camargo Biobank. Our results showed no significant change in RIN values according to length of storage at -140°C. With respect to RNA aliquots stored at -80°C, RNA integrity at 250 ng/μL was preserved, while statistically significant degradation was observed at 25 ng/μL after only 8 months of storage. The RNA collection from most of the human tumors stored at the AC Camargo Biobank exhibited high quality, with average RIN around seven. However, ovary and stomach samples had the greatest RNA degradation. Taken together, the results show that both the temperature of preservation and the concentration of RNA should be strictly controlled by the biobank staff involved in macromolecule purification. Moreover, the RNAs from our biobank can be useful for the most demanding methods of gene expression analysis by virtue of adherence to optimal standard operating procedures for both tissue and macromolecule laboratories.

Chlorophyll as an indicator of green tea quality

The objective of this work was to study the changes in chlorophyll contents in green teas stored under different conditions and to suggest the most suitable way and length of green tea storage. A secondary aim was to evaluate the possibility of using the chlorophyll content as an indicator of tea freshness. Samples for analysis were obtained from a range of tea-growing regions. Chlorophyll contents were measured using UV-vis spectrophotometry. Chlorophyll absorbance was measured at 642.5 and 660 nm. Prior to storage, chlorophyll concentration in teas ranged from 1.12 to 1.89 mg per 1 g of tea, which corresponded to data given by other authors. Storage was found to lead to a decline in chlorophyll concentration. From the 6th month of storage onwards, a significant decrease (P &lt; 0.05) was observed in all samples, irrespective of storage conditions. Based on the findings of this study, original and metal packaging can be considered the most chlorophyll-friendly. On the other hand, glass and paper packaging stored in direct daylight were found to have the strongest impact on chlorophyll concentration. Overall, it can be concluded that the storage time can have a strong influence on oolong green tea colour as an important qualitative variable. Thus, the shelf-life of tea should be shorter than the 12 months claimed by most manufacturers. Once the relationship between changes in chlorophyll concentration and length of the storage period had been established, the chlorophyll content was suggested as an indicator of the storage time (freshness) of a tea substance.

Germination of tropical forage seeds stored in ambient and controlled temperature and humidity conditions

Correct storage of tropical forage seeds in the humid tropics is critical in order to safeguard germination. Storing Mulato hybrid brachiaria (Brachiaria ruziziensis x B. brizantha) seed under ambient conditions could be safely done for 8−12 months in Thailand, but rapid deterioration in viability occurred with longer storage, with seed being totally non-viable after 20 months storage (Hare et al. 2008). However, Mulato seeds kept in cold storage (10 C and 40% RH) for 3 years still maintained 80% germination. Similar results were found in northern Australia (Hopkinson and English 2005), where loss of viability of Panicum maximum, Brachiaria decumbens, Brachiaria humidicola, Setaria sphacelata and Chloris gayana seeds was rapid under ambient conditions with total death at 3 years. Under cold storage, maximum seed viability was still maintained after 6 years of storage. Embryo dormancy is reduced with age (length of storage) and is usually short-lived (i.e. several months) in many Brachiaria species. Hare et al. (2008) found that dormancy was quickly lost in Mulato seed stored at ambient temperatures but still persisted strongly after 3 years in cold storage. Hopkinson and English (2005) found that dormancy persisted longer under cool storage than under ambient storage. Forage seeds produced by Ubon Forage Seeds at Ubon Ratchathani University (Hare et al. 2013) are stored in a large commercial cool room (18−20 C and 50% RH), but there are no data on how long seeds can be safely stored in this room. The objectives of this trial were to study: the effects of ambient storage and cool-room storage on germination of our range of tropical forage seeds; and the persistence of embryo dormancy with storage.

The Harvest and Post-harvest of Traditional Pear Varieties in Hungary

We have viewed a business in Hungary from an economic point of view. The main activity there is pear growing and storage. Four varieties of different time of ripening and storing are grown there. We have measured all the relevant activities, worked out local normative and prepared a detailed technology. The economic evaluation was based on this data. Activities, like disinfection, pre-storage disinfection and selection, in-storing and out-storing, classification after storage, packaging, as well as loading trucks, were monitored by variety. Storage loss was determined and widely varied according to varieties, length of storage and time of out-storing. Different varieties resulted in different quality classes after storage. Price depended on the quality classes. Economic evaluation was carried out when all the relevant costs and revenues were known. Fixed and variable costs of storage were determined, break-even point was calculated and the market position of the product was evaluated.

Crossover Roundabouts

The double crossover diamond (DCD) (also known as the diverging diamond interchange) was first introduced conceptually to engineers and planners in the United States less than a decade ago. The concept has been spreading rapidly since that time; as of July 2013, DCDs had been constructed in 19 locations in eight states and had been studied in countless other projects nationwide. Nearby frontage roads pose operational challenges for all interchanges, and DCD geometry intensifies this problem. Reverse curvature for crossover points reduces the storage length between ramps and nearby frontage roads in DCDs. The crossover roundabout concept was conceived as a potential solution to this challenge. Instead of a signalized crossover point (as in a DCD), a five-legged roundabout was proposed for the intersecting arterial, frontage road, and ramps. A crossover roundabout would function essentially like any other roundabout except that it would adjoin an arterial roadway with travel directions flipped on one side. The operational and safety benefits of typical roundabouts are expected to apply to crossover roundabouts; no new driver behaviors must be learned. Variations of crossover roundabout interchanges are possible: one crossover roundabout could adjoin half of a DCD, or two crossover roundabouts could form a complete interchange. Interchanges with two crossover roundabouts would look like typical double-roundabout diamond interchanges with the arterial road directions flipped between the roundabouts. Crossover roundabouts are not required to contain frontage roads to function. Microsimulation and cost estimations have shown the concept to have merit; crossover roundabouts can combine the best attributes of DCDs and roundabouts.

Changes in volumetric energy densities during storage of whole-tree feed stocks from silvicultural thinnings

The quality issues of woody feed stocks usually report changes in the moisture content, heating value or dry matter, but these factors are rarely summed and reported as changes in energy densities for a given feed stock. This paper reports the results of ten whole-tree and two stem wood storage field trials on energy densities. The material consisted of eight Scots pine (P. sylvestris), and four downy birch (B. pubescence) trials, all after the first thinnings. Other factors studied were the length of storage, pile cover and seasoning in the stand. The two variables under scrutiny were the basic density and moisture content. It was shown that even a good degree of drying could not counterbalance the reduction in the basic density. The Scots pine feed stocks dried well, and the basic density losses were less than those of downy birch. As a result, the pine feed stocks increased in energy density from 0.7 to 17.6% over the trial period, whereas all of the birch feed stocks suffered losses from −3.4 to −9.6%. Although covering the feed stocks had a beneficial effect on moisture content, it proved to have a positive effect only on pine when the storage was extended beyond one year. There was some gain on energy density if the feed stocks were seasoned in the stand, but this advantage was lost at the roadside landing. Furthermore, transpiration drying in the stand requires a two-phase harvesting technique, and this expense may be difficult to justify.

Quantitative analysis of flavonoids, sugars, phenylalanine and tryptophan in onion scales during storage under ambient conditions.

A comprehensive quantitative analysis of flavonoids, sugars, phenylalanine, and tryptophan have been carried out in different onion scales during storage at ambient temperature (20-23°C) and relative humidity (60-80%). Depending on the length of storage, dry matter content and composition shows variation inside the onion bulbs. Inner sprouts were observed on longitudinally cut bulbs after 2months and visible sprouts appeared after 5months of storage. The bulbs lost 20 to 30% of their weight at the end of the storage. Higher dry matter content was observed in the inner scales. Significantly high content of quercetin in inner scales and high level of quercetin-3,4'-O-diglucoside and quercetin-4'-O-monoglucoside in outer scales was observed during a 7months storage. During storage period, high content of fructose and glucose was observed in the middle scales while sucrose was high in the inner scales. There was no particular trend observed within analyzed amino acids. However, the content of phenylalanine was higher than tryptophan.

Effects of extended period of storage and strain of layer on quality of chicken eggs

Poultry farmers in Ghana use different strains of layers for egg production with eggs produced being stored for some time before use. This has necessitated a study to determine the effect of strain of layers and extended storage length on internal and external qualities of chicken eggs. A total of three-hundred (300) eggs from 46-week old layer strains were analysed for egg quality in a 3 X 5 factorial experiment involving three layer strains (Lohmann brown, Lohmann white and Black Bovan) and 5 storage periods of 0, 5, 10, 15 and 20 days. On each day of the analysis, sixty (60) eggs; 20 each from the three strains were randomly sampled and analysed for the internal and external egg characteristics. Results obtained showed a significant difference (P&lt;0.05) among the three layer strains regarding the major egg quality traits like egg weight, albumen weight, haugh unit and yolk weight with the Black Bovan layer strains being superior to the Lohmann strains. It was also observed that the major egg quality traits like egg weight, albumen weight and haugh unit were significantly (P&lt;0.05) affected with increasing storage length. There was also a significant (P&lt;0.05) interaction effects between strain of layer and length of storage of egg on some important egg quality determinants like egg weight, albumen height and haugh unit. This shows that in order not to compromise the quality of eggs, one must consider the strain of layer to use and how long eggs could be stored before use. Irrespective of the strain of layers and storage period, it was realized that egg weight was more closely associated (P&lt;0.05) with albumen height and weight than the other traits.Keywords:Keywords: Albumen height, egg quality, egg weight, haugh unit, storage length, strain