Storage In Saliva Research Articles

Assessment of surface microhardness of enamel surface treated with Nano- HAP serum before and after bleaching

Background: Although bleaching is typically considered a safe procedure, various investigations have found minor negative effects and changes in mineral composition. The aim was to Evaluate and compare the efficacy of using Nanohydroxyapatite serum on surface microhardness of enamel surface before and after bleaching with chemically cured Boost bleaching. Material and methods: ten sound human permanent upper and lower premolar teeth were used and their roots were removed 2 mm apically to the cementoenamel junction, the crowns were sectioned mesiodistally into two halves buccal and lingual/palatal, the buccal surface was further subdivided into two halves. The samples were embeded in an acrylic resin, resulting in 30 specimens divided into 3 groups: Control group: using Boost bleaching and stored in artificial saliva for 14 days, prevention group: nanohydroxyaptite (n-HAP) serum applied 2-3 min once daily for 10 days followed by bleaching then stored for 14 days in artificial saliva and treatment group: in which bleaching used before nanohydroxyapatite serum and stored in artificial saliva for 14 days. The samples were subjected to a Vickers microhardness test measured at 4 times: base line, after nanohydroxyapatitem, after one day of bleaching and after storage in artificial saliva for 14 days in all groups. The data were analyzed statistically using repeated analysis of variance (ANOVA) test followed by Tukey's test. Results: there was a significant increase in microhardness in the prevention group (p<0.05) while there was no significant difference in microhardness readings in control and treatment groups (p> 0.05). Conclusion: n-HAP may enhance the microhardness of a bleached enamel surface when used as a preventive & treatment measure. Suggested that a higher increase in enamel microhardness occurs when n-HAP is used for 2-3 min once daily for 10 days before bleaching and maintaining this increase even after storage for 14 days in artificial saliva.

Nanoindentation creep: The impact of water and artificial saliva storage on milled and 3D-printed resin composites.

This study evaluated the effects of artificial saliva and distilled water on the nanoindentation creep of different 3D-printed and milled CAD-CAM resin composites. Disk-shaped specimens were subtractively fabricated from polymer-infiltrated ceramic network (EN) and reinforced resin composite (B) and additively from resin composite (C) and hybrid resin composite (VS) using digital light processing (DLP). Specimens from each material were divided into two groups according to their storage conditions (artificial saliva or distilled water for 3 months). Creep was analyzed by nanoindentation testing. Statistical analysis was done using two-way ANOVA, one-way ANOVA, Bonferroni post hoc tests, and independent t-test (α = 0.05). The main effects of material and storage conditions, and their interaction were statistically significant on nanoindentation (p<0.001). Storage condition had the greatest influence (partial eta squared ηP 2 =0.370), followed by the material (ηP 2 = 0.359), and the interaction (ηP 2 = 0.329). The nanoindentation creep depths after artificial saliva storage ranged from 0.34 to 0.51µm and from 0.50 to 0.87µm after distilled water storage. One of the additively manufactured groups had higher nanoindentation creep depths in both storage conditions. All specimens showed comparable performance after artificial saliva storage, but increased nanoindentation creep after distilled water storage for 3 months. The subtractive CAD-CAM blocks showed superior dimensional stability in terms of nanoindentation creep depths in both storage conditions. Additively manufactured composite resins had lower dimensional stability than one of the subtractively manufactured composites, which was demonstrated as having higher creep deformation and maximum recovery. However, after artificial saliva storage, one of the additively manufactured resins had dimensional stability similar to that of subtractively manufactured.

Marginal integrity of prototype bioactive glass-doped resin composites in class II cavities.

This in vitro study examined the marginal integrity of experimental composite materials doped with bioactive glass (BG). Class-II MOD cavities were prepared and restored with one of the following composite materials: a commercial composite material as a reference (Filtek Supreme XTE), an experimental composite doped with BG 45S5 (C-20), and an experimental composite doped with a fluoride-containing BG (F-20). Six experimental groups (n = 8) were used, as each of the three composites was applied with (+) or without (-) a universal adhesive (Adper Scotchbond Multipurpose). All specimens were subjected to thermocycling (10,000 x, 5-55°C) and then additionally stored in artificial saliva for eight weeks. Scanning electron micrographs of the mesial and the distal box were taken at three time points (initial, after thermocycling, and after eight weeks of storage in artificial saliva). The margins were classified as "continuous" and "non-continuous" and the percentage of continuous margins (PCM) was statistically analyzed (α = 0.05). In most experimental groups, thermocycling led to a significant decrease in PCM, while the additional 8-week aging had no significant effect. F-20 + performed significantly better (p = 0.005) after 8 weeks storage in artificial saliva than the reference material with adhesive, while no statistically significant differences were observed at the other two time points. C-20 + exhibited significantly better PCM than the reference material with adhesive after thermocycling (p = 0.026) and after 8 weeks (p = 0.003). Overall, the experimental composites with BG showed at least as good marginal adaptation as the commercial reference, with an indication of possible re-sealing of marginal gaps. Maintaining or improving the marginal integrity of composite restorations is important to prevent microleakage and its likely consequences such as pulp irritation and secondary caries.

Construction of Zwitterionic Coatings with Lubricating and Anti-Adhesive Properties for Invisible Aligner Applications.

Invisible aligners have been widely used in orthodontic treatment but still present issues with plaque formation and oral mucosa abrasion, which can lead to complicated oral diseases. To address these issues, hydrophilic poly(sulfobetaine methacrylate) (polySBMA) coatings with lubricating, antifouling, and anti-adhesive properties have been developed on the aligner materials (i.e., polyethylene terephthalate glycol, PETG) via a simple and feasible glycidyl methacrylate (GMA)-assisted coating strategy. Poly(GMA-co-SBMA) was grafted onto the aminated PETG surface via the ring-opening reaction of GMA (i.e., "grafting to" approach to obtain G-co-S coating), or a polySBMA layer was formed on the GMA-grafted PETG surface via free radical polymerization (i.e., "grafting from" approach to obtain G-g-S coating). The G-co-S and G-g-S coatings significantly reduced the friction coefficient of PETG surface. Protein adsorption, bacterial adhesion, and biofilm formation on the G-co-S- and G-g-S-coated surfaces were significantly inhibited. The performance of the coatings remained stable after storage in air or artificial saliva for 2 weeks. Both coatings demonstrated good biocompatibility in vitro and did not cause irritation to the oral mucosa of rats in vivo over 2 weeks. This study proposed a promising strategy for the development of invisible aligners with improved performance, which is beneficial for oral health treatment. This article is protected by copyright. All rights reserved.

Effects of printing orientation and artificial ageing on martens hardness and indentation modulus of 3D printed restorative resin materials

BackgroundThree-dimensional (3D) printing is increasingly used to fabricate dental restorations due to its enhanced precision, consistency and time and cost-saving advantages. The properties of 3D-printed resin materials can be influenced by the chosen printing orientation which can impact the mechanical characteristics of the final products. PurposeThe objective of this study was to evaluate the influence of printing orientation and artificial ageing on the Martens hardness (HM) and indentation modulus (EIT) of 3D-printed definitive and temporary dental restorative resins. MethodsDisk specimens (20 mm diameter × 2 mm height) were additively manufactured in three printing orientations (0°, 45°, 90°) using five 3D-printable resins: VarseoSmile Crownplus (VCP), Crowntec (CT), Nextdent C&B MFH (ND), Dima C&B temp (DT), and GC temp print (GC). The specimens were printed using a DLP 3D-printer (ASIGA MAX UV), while LavaTM Ultimate (LU) and Telio CAD (TC) served as milled control materials. Martens hardness (HM) and indentation modulus (EIT) were tested both before and after storage in distilled water and artificial saliva for 1, 30, and 90 days at 37 °C. Results90° printed specimens exhibited higher HM than the other orientations at certain time points, but no significant differences were observed in HM and EIT between orientations for all 3D-printed materials after 90 days of ageing in both aging media. LU milled control material exhibited the highest HM and EIT among the tested materials, while TC, the other milled control, showed similar values to the 3D printed resins. CT and VCP (definitive resins) and ND displayed higher Martens parameters compared to DT and GC (temporary resins). The hardness of the 3D-printed materials was significantly impacted by artificial ageing compared to the controls, with ND having the least hardness reduction percentage amongst all 3D-printed materials. The hardness reduction percentage in distilled water and artificial saliva was similar for all materials except for TC, where higher reduction was noted in artificial saliva. SignificanceThe used 3D printed resins cannot yet be considered viable alternatives to milled materials intended for definitive restorations but are preferable for use as temporary restorations.

Polarization-Sensitive Optical Coherence Tomography for Monitoring De- and Remineralization of Bovine Enamel In Vitro.

Early caries diagnosis still challenges dentistry. Polarization-sensitive optical coherence tomography (PS-OCT) is promising to detect initial lesions non-invasively in depth-resolved cross-sectional visualization. PS-OCT with determined degree of polarization (DOP) imaging provides an intuitive demineralization contrast. The aim of this study is to evaluate the suitability of DOP-based PS-OCT imaging to monitor controlled de- and remineralization progression for the first time and to introduce it as a valid, non-destructive in vitro detection method. Twelve standardized bovine enamel specimens were divided in different groups and demineralized with hydrochloric acid (HCl) as well as partly remineralized with fluoride over a 14-day pH-cycling experiment. The specimens were stored in artificial saliva and sodium chloride (NaCl), respectively. Progress measurements with PS-OCT were made with polarization-sensitive en faceand B-scan mode for qualitative evaluation. The specimens demineralized in HCl showed the most pronounced surface change (lowest DOP) and the most significant increase in depolarization. Additional fluoride treatment and the storage in artificial saliva resulted in the opposite (highest DOP). Therefore, DOP-based PS-OCT imaging appears to be a valuable technique for visualization and monitoring of enamel demineralization and remineralization processes in vitro. However, these findings need to be confirmed in human teeth ex vivo or in situ.

Impact of Artificial Aging on the Physical and Mechanical Characteristics of Denture Base Materials Fabricated via 3D Printing.

Three-dimensional (3D) printing is becoming more prevalent in the dental sector due to its potential to save time for dental practitioners, streamline fabrication processes, enhance precision and consistency in fabricating prosthetic models, and offer cost-effective solutions. However, the effect of aging in artificial saliva of this type of material has not been explored. To assess the physical and mechanical properties of the two types of 3D-printed materials before and after being subjected to artificial saliva, a total of 219 acrylic resin specimens were produced. These specimens were made with two types of 3D-printed materials, namely, NextDent (ND) and Formlabs (FLs), and a Schottlander heat-cured (HC) resin material that was used as a control. Water sorption and solubility specimens (n = 5) were tested after three months of storage in artificial saliva. Moreover, the Vickers hardness, Martens hardness, flexural strength/modulus, and impact strength were evaluated both under dry conditions and after three months of storage in artificial saliva. The degree of conversion (DC), elemental analysis, and filler content were also investigated. The ANOVA showed that 3D-printed resins had significantly greater sorption than the control group (p < 0.05). However, the flexural strength values of the 3D-printed materials were significantly greater (p < 0.05) than those of the heat-cured material. The DC of the 3D-printed resins was lower than that of the control group, but the difference was not significant (p > 0.05). The 3D-printed materials contained significantly more filler than the control (p < 0.05). Moreover, the artificial saliva had a significant effect on the Vickers hardness for all tested groups and on the Martens hardness for the control group only (p < 0.05). Compared with conventional heat-cured materials, 3D-printed denture base materials demonstrated relatively poorer performance in terms of sorption, solubility, and DC but exhibited either comparable or superior mechanical properties. The aging process also influenced the Vickers and Martens' hardness. The strength of the 3D-printed materials was in compliance with ISO recommendations, and the materials could be used alongside conventional heat-cured materials.

Supplementation of Energy Drinks with Green Tea Extract: Effect on In Vitro Abrasive/Erosive Dentin Wear.

To investigate the effect of the supplementation of energy drinks with green tea extract on abrasive and erosive dentin wear. Six groups, each comprising 15 bovine dentin samples, were prepared, yielding a total of 90 samples. Erosion was performed by immersing the samples in Red Bull and Red Bull light with and without green tea extract. Tap water with and without green tea extract was used as the control groups. The samples were subjected to abrasive/erosive cycling for five days. The following cycling was performed daily: toothbrush abrasion (20 brushstrokes; 2.5 N); eight erosive cycles (2 min storage in the respective solutions); in between the erosive cycles, storage in artificial saliva (60 min) and again toothbrush abrasion (20 brushstrokes; 2.5 N). During the night, samples were again stored in artificial saliva. Abrasive/erosive dentin wear was measured using a stylus profilometer (µm, accuracy = 40 nm). The measured dentin loss results from the vertical position shift on the y-axis from base to final profile after the wear process in 2D. Pairwise comparisons between the groups were carried out using Wilcoxon signed-rank test. The following dentin wear (median [IQR]) was measured: Red Bull: 1.9 µm (0.5); Red Bull Light: 1.3 µm (0.3); Red Bull with green tea extract: 0.8 µm (0.3); Red Bull Light with green tea extract: 0.3 µm (0.5); Tap water with green tea extract: -0.2 µm (0.7); Tap water: -1.0 µm (1.2). The comparison of all tested groups to each other proved to be statistically significant (p < 0.05). The supplementation of energy drinks with green tea extract provide a protective effect against erosive/abrasive wear in vitro.

Concentration effect of DMSO-dry bonding on the stability of etch-and-rinse bonds

ObjectivesTo examine whether lower dimethyl sulfoxide (DMSO) concentrations would affect long-term bond stability of simplified or multistep water-based adhesives to dry-etched dentin. MethodsH3PO4-etched mid-coronal dentin surfaces from human molars were randomly blot- or air-dried for 30 s and pretreated or not with 5 or 50 % (v/v) ethanolic DMSO solutions. Untreated samples served as control. Samples were bonded with a two-step or a three-step etch-and-rinse adhesive. Restored crown segments (n = 5/group) were stored in distilled water for 24 h and sectioned for microtensile bond strength testing. Resin-dentin beams (0.8 mm2) were tested under tension until fracture (0.5 mm/min) after 24 h and one year of storage in artificial saliva at 37 °C. Nanoleakage evaluation and hybrid layer characterization were performed by SEM. Bond strength data was examined by three-way ANOVA followed by the Tukey test (α = 0.05). Results: Dry bonding produced significantly lower bond strengths than conventional wet bonding for both water-based adhesive systems (p < 0.05). DMSO-dry bonding restored bond strengths and reduced nanoleakage levels, regardless of adhesive type or DMSO concentration (p < 0.05). Bond strengths of DMSO-dry bonded samples were not significantly affected by long-term ageing regardless of adhesive type or DMSO concentration (p < 0.05). SignificanceAlthough bonding methacrylate-based resins to etched dentin is normally performed under wet conditions, hybridization of air-dried collagen can outperform conventional wet bonding by employing water-free DMSO solutions with concentrations as low as 5 %. Reduced moisture-related technique sensitivity, higher bonding performance and improved hybrid layer stability may contribute to extend the service life of resin-dentin bonding.

A comparative evaluation of the effect of alcoholic and non alcoholic beverages on tooth enamel surface pretreated with β-tricalcium phosphate, bioactive glass and amine fluoride: an in vitro study

Background and aims. The aim of this in vitro study is to quantitatively evaluate the effect of different alcoholic and non alcoholic beverages on the tooth enamel surface topography pretreated with various remineralizing agents using Atomic Force Microscopy.&#x0D; Methods. 120 tooth specimens were prepared from 60 freshly extracted intact human premolars by sectioning from mesial to distal surfaces using low speed diamond discs and were randomly assigned to study groups and control group. Specimens of Group I, Group II and Group III were pre-treated with β-Tri calcium phosphate, bioactive glass and amine fluoride respectively for 4 minutes for 28 days, followed by storage in artificial saliva. All the specimens were evaluated for surface roughness using Atomic Force Microscopy. The specimens were then placed in alcoholic and non-alcoholic beverages for 10 minutes for 4 days and were again analyzed by Atomic Force Microscopy.&#x0D; Descriptive statistics was performed by using the proportional or frequency distribution of the parameters. The respondents were then grouped according to the branch of specialty if any and the data was evaluated by the one-way ANOVA with post-hoc, with p value &lt;0.005.&#x0D; Results. In the present study, among the remineralizing agents tested, bioactive glass was found to be more effective than β-Tri Calcium Phosphate and Amine Fluoride. Among the demineralizing agents used in this study, the demineralization potential of Coca Cola was found to be highest, followed by wine and green tea pretreated with β -tricalcium phosphate, bioactive glass and amine fluoride.&#x0D; Conclusions. The present study concluded that all the remineralizing agents tested were found to be effective in inhibiting the demineralization caused by various alcoholic and non alcoholic beverages. Among the remineralizing agents tested, bioactive glass was found to be more effective than β-tri calcium phosphate and amine fluoride.

Evaluation of Microtensile Bond Strength between SMART Technique and Conventional Glass Ionomer Cement-treated Carious Primary Dentin.

Silver diamine fluoride (SDF) solution has been used clinically to prevent and arrest dental caries. To evaluate the microtensile bond strength between silver-modified atraumatic restorative technique (SMART) and glass ionomer cement (GIC) applied to carious primary teeth and also the mode of restoration failure. A total of 40 carious primary molars were equally allocated into test and control groups after sectioning through the middle of the carious lesion. The test specimens were treated with one drop of 38% SDF and the control with deionized water. The samples were stored in artificial saliva for 14 days at 37° C and the dentin surfaces were conditioned and restored with Fuji IX GP Extra. After 24 hours storage in artificial saliva, the specimens were prepared for testing of microtensile bond strength. Paired t-test was used to compare the mean bond strengths. The mode of failure was assessed with a stereomicroscope under 40× magnification. The mean microtensile strength for the test group was 7.39 MPa [standard deviation (SD ± 2.3)] and 7.20 MPa (SD ± 1.98) for the control group (p > 0.05). The most common mode of failure was the mixed failure mode in both groups. Silver diamine fluoride (SDF) does not adversely affect the bond strength between GIC applied to carious dentin. Velagala D, Reddy A, Reddy VN, et al. Evaluation of Microtensile Bond Strength between SMART Technique and Conventional Glass Ionomer Cement-treated Carious Primary Dentin. Int J Clin Pediatr Dent 2023;16(4):582-586.

Colour Parameters and Changes of Tea-Stained Resin Composite Exposed to Whitening Pen (In Vitro Study).

One of the crucial factors influencing the longevity of anterior aesthetic dental restorations is the colour stability of resin composites. Bleaching and whitening have become popular methods for enhancing dental aesthetics. Home whitening techniques, such as special pens, are widely available commercially. This in vitro study aimed to determine the efficiency of a whitening pen in removing tea stains from resin composite by measuring colour differences (ΔE00). Additionally, the study aimed to evaluate the variations in colour parameters measured by extra-oral and intra-oral spectrophotometers. A total of 45 disc-shaped resin composite specimens were randomly divided into three groups; Group 1: stored in artificial saliva (control), Group 2: stored in artificial saliva followed by a whitening pen application, and Group 3: stored in tea followed by a whitening pen application. Colour measurements were taken three times for each specimen using two spectrophotometers (extra-oral and intra-oral devices); T1: before storage (baseline), T2: after storage in artificial saliva or tea for 6 days; and T3: after one week of whitening pen application in groups 2 and 3. The data were statistically analyzed using one-way ANOVA followed by the Tukey post hoc test (p ≤ 0.05). The independent sample t-test was also employed. The equation of CIEDE2000 (ΔE00) was used to calculate the colour difference between the dry, as-prepared specimens (baseline), and those after storage or bleaching. The colour changes exceeding the acceptability threshold (∆E00 = 1.8) were considered unacceptable. After whitening, the colour of the specimens stored in brewed tea (Group 3) remained unacceptable, as indicated by both the extra-oral and intra-oral spectrophotometers (ΔE00 = 4 and 2.9, respectively). Groups 1 and 2 exhibited lower ΔE00 values than Group 3 (p = 0.01 *). No significant difference was observed between Group 1 (stored in artificial saliva) and Group 2 (stored in artificial saliva and then bleached) (p = 0.3). Significant differences were consistently observed between the data obtained from the extra-oral spectrophotometer and the intra-oral one. The whitening pen proved ineffective in removing tea stains from resin composites. Although significant differences were found between the values obtained by the two spectrophotometers (extra-oral and intra-oral), both devices confirmed the unacceptable colour of the tea-stained resin composites after whitening.

Microhardness and surface roughness of resin infiltrated bleached enamel surface using atomic force microscopy: An in vitro study

Aimto evaluate the microhardness (VHN) and surface roughness (Ra) of human enamel surface treated with resin infiltration followed by in office bleaching and to study the effect of artificial saliva (AS) storage time on the VHN and Ra of resin infiltrated enamel. Materials and methods: Eighty enamel specimens were prepared from extracted human premolar teeth. Specimens were divided into two main groups (I and II) then, they were demineralized to create white spot lesions (WSLs). Group I was divided into A and B sub groups. Group A was treated with in office bleaching material while for group B, resin infiltration (Icon) was applied after 24 h storage in AS followed by bleaching. Group II was divided into (C and D). Group C was treated like group B. Specimens in group D were stored in AS for 14 d after treatment with Icon and before application of bleaching. Atomic force microscopy (AFM) Ra analysis and VHN calculation were done. Independent T Test was used to compare between groups at P < 0.05. Results: Group B demonstrated a significant increase in VHN compared with group A. Storage time in AS showed no significant difference on VHN or Ra between groups. Conclusion: Application of resin infiltration before bleaching significantly increased VHN and slightly improved Ra of WSLs compared with bleaching alone. Application of bleaching material after 24 h or 14 days of the application of resin infiltration did not have a significant effect or surface hardness or roughness of WSLs.

Mucoadhesive buccal tablet of leuprolide and its fatty acid conjugate: Design, in vitro evaluation and formulation strategies

This study aimed to design mucoadhesive buccal tablets of leuprolide (LEU) and to manufacture and evaluate the properties of buccal tablets containing LEU-oleic acid conjugate (LOC) and self-assembled LEU-oleic acid nanoparticles (LON), which were developed in a previous study. Hydroxypropyl methylcellulose (HPMC 4000) was used as the mucoadhesive polymer, and tablets were prepared by direct compression. The formulations were characterized by weight, content uniformity, thickness, hardness, swelling index, disintegration time, mucoadhesion time, and drug release. The chosen formulation maintained an adhesion time of up to 6.43 h and a disintegration time of 4.10 h. Drug stability in the mucoadhesive tablets was confirmed after 2 h of storage in human mimic saliva (Phosphate buffer solution pH 6.8). Furthermore, the designed LEU formulation and the LOC and LON developed in a previous study were prepared as buccal tablets and compared. In the dissolution and permeation studies, LON-loaded buccal tablets showed the highest permeation rate. This study suggests that mucoadhesive buccal tablets containing self-assembled LON may effectively increase the medication adherence for pediatric and geriatric patients by improving the bioavailability and permeation rate of LEU.

COLOR CHANGES OF 3D PRINTED AND CONVENTIONAL DENTAL RESINS FOR REMOVABLE PROSTHODONTICS AFTER IMMERSION IN DIFFERENT STAINING AGENTS

Introduction: The discoloration of the denture base resins is one of the shortcomings of this type of dental materials, and it could be determined through various visual and instrumental methods. The aim of the current study was to evaluate the color stability of 3Dprinted and conventional denture base resins after immersion in different staining solutions. Materials and methods: A total of 200specimens were manufactured from two types of materials: 3D printed dental resin NextDent Denture 3D+ (NextDent, 3D Systems, The Netherlands) and heat-polymerized PMMA Vertex (3D Systems, The Netherlands), which were immersed in four types of colorants - artificial saliva, coffee, red wine and coke (n = 25). For measuring the color changes (CIE-L*a*b* system) of all specimens after storage in artificial saliva for 24 h at 37 degrees C (T0), a spectrophotometer SpectroShade Micro (SpectroShade, USA) was used. After seven days (T1), 14 days (T2) and 21 days (T3), the mean ∆E values were calculated and compared by Bonferonni post-hoc test. The data were processed using thestatistical software SPSS 26. The level of significance forrejecting the null hypothesis was fixed at p&lt;0,05. Results: The values for ΔE were investigated, and the interactions between the type of material,the immersion time and the different staining solutions were statistically significant. The highest mean for ∆E was evaluated for both types of dental resin in red wine. Respectively, 3D printed specimens in artificial saliva were with the lowest mean. Conclusions: The 3D printed denture base resin demonstrated better color stability than the conventional acrylic materials. The staining effect correlated with the immersion time, with the red wine and coke being with the most chromogenic impact and the period with the highest color changes being 21 days.There was a significant interaction between theselected time periods and the type of staining agent, as both types of dental materials showedchanges in color stability at T1 compared to T3.

Comparative Evaluation of Flexural Strength, Modulus of Elasticity, and Microleakage of Three Different Glass Ionomer Restorative Materials in Class V Preparations – An In vitro Study

Aims: Flexural strength, modulus of elasticity, and microleakage evaluation of three glass ionomer restorative materials in class V preparations. Materials and Methods: Twenty bar-shaped specimens each of Type-II glass-ionomer cement (GIC), Zirconomer and Type-IX GIC were prepared by inserting freshly mixed cements into 25 mm × 2 mm × 2 mm polyvinylacetate moulds followed by storage in artificial saliva for 24 h at 37°C. Samples were then rinsed, polished, and tested in autograph universal testing machine for flexural strength and modulus of elasticity using three-point bending test. Standardized Class V cavities in 60 extracted human premolars of dimensions 3 mm × 2 mm × 1.5 mm were prepared, divided at random into three groups of twenty each and restorations were done using Type-IX GIC, Type-II GIC, and Zirconomer. Teeth were immersed in 1%wt Rhodamine-B dye after thermocycling for 24 h, split bucco-lingually in longitudinal section and evaluated for microleakage under a stereomicroscope. Results: Flexural strength evaluation showed that Zirconomer had the highest and Type-II GIC had the lowest flexural strengths that was statistically significant (P &lt; 0.05; analysis of variance [ANOVA]-Tukey Honestly Significant Difference [HSD]). Analysis of modulus of elasticity revealed that Type-IX GIC had the highest and Type-II GIC had the lowest modulus and the difference was significant statistically (P &lt; 0.05; ANOVA-Tukey HSD). Occlusal microleakage was the highest in samples restored with Zirconomer (50% of the teeth), whereas samples restored with Type IX GIC showed no microleakage. At the gingival margins, 60% of both Type-IX GIC and Zirconomer restored samples exhibited no microleakage and 55% of Type-II GIC restored samples showed no microleakage. Conclusion: Within the limitations of this study, Type IX GIC exhibited good flexural strength, low modulus of elasticity, and least microleakage followed by Type II GIC and Zirconomer.

Effect of a primer containing Artemisinin on dentin endogenous MMPs and resin bond durability

ObjectivesThis study aims to investigate the potential effect of a primer containing Artemisinin (ART) or its derivative—dihydroartemisinin (DHA) on the dentin endogenous MMPs activity and resin bonding durability under etch-and-rinse mode. Materials and methodsMolecular docking was applied for evaluating the potential interactions of MMP-2 and MMP-9 with ART and DHA. Then human third molars were randomly assigned to six groups, after etch-and-rinse treatment, pretreated with the primers containing 0.25, 0.5 mg/mL ART, 0.25, 0.5 mg/mL DHA and 2% chlorhexidine (CHX) for 2 min, respectively, while one untreated group was served as the control group. The endogenous MMPs activity of demineralized dentin was tested with in situ zymography test and zymographic assay. Following the etch-and-rinse bonding process with a universal resin bond, the specimens of the microtensile bond strength (μTBS) and nanoleakage were tested with or without 10,000 °C thermocycling and 6-month storage in artificial saliva at 37 °C. ResultsMolecular docking showed that ART and DHA combined with MMP-2 and MMP-9 through forming covalent bonds with the zinc ion of MMPs. Compared with the control group, the ART, DHA and CHX groups showed less endogenous MMPs activity, and the MMPs activity of 0.5 mg/mL DHA group had no significant difference from that of 2% CHX group. Ageing condition reduced the μTBS values dramatically and also aggravated the nanoleakage of all the groups, specifically, the control group showed lower μTBS value and a broader, thicker and more consecutive silver deposition than other groups. ConclusionsThe primer containing ART or DHA could preserve bond durability and slow down the nanoleakage of resin-dentin interface over time through inhibiting the endogenous MMPs activity.

Incorporation of bioactive glass-ceramic into coconut oil for remineralization of incipient carious lesions.

This study evaluated the efficacy of incorporating different concentrations of bioactive glass-ceramic (Biosilicate) into coconut oil on the remineralizing potential and surface roughness of white spot lesions. Fragments (6 x 6 x 2mm) of bovine teeth were sectioned and initial microhardness (KHN) and surface roughness (Ra) readings were obtained. The samples were submitted to cariogenic challenge to form white spot lesions and were separated into six groups (n=13): 1) Artificial Saliva (AS); 2) Coconut Oil (CO); 3) CO+2% Biosilicate (CO+2%Bio); 4) CO+5% Biosilicate (CO+5%Bio); 5) 2% Biosilicate Suspension (2% Bio) and 6) 5% Biosilicate Suspension (5% Bio). The treatments for 1 cycle/day were: immersion into the treatments for 5 minutes, rinsing in distilled water, and storage in artificial saliva at 37ºC. After 14 days, KHN and Ra readings were taken. The surface roughness alteration ((Ra) was analyzed (Kruskal-Wallis, Dunn's post-test, p<0.05). CO+2%Bio had higher (p = 0.0013) (Ra followed by CO+5%Bio (p = 0.0244) than AS. The relative KHN and remineralization potential were analyzed (ANOVA, Tukey, p<0.05), and 5% Bio treatment presented a higher relative microhardness than all other groups (p>0.05). The remineralizing potential of all the treatments was similar (p > .05). When Biosilicate was added, the pH of the suspensions increased and the alkaline pH remained during the analysis. Biosilicate suspension is more efficient than the incorporation of particles into coconut oil at white spot lesion treatment. In addition to the benefits that coconut oil and Biosilicate present separately, their association can enhance the remineralizing potential of Biosilicate.

Development of 3D printed dental resin nanocomposite with graphene nanoplatelets enhanced mechanical properties and induced drug-free antimicrobial activity.

Oral prosthetic rehabilitation has been used for a long time to restore function and natural appearance; however, it is still one of the most challenging areas in dentistry due to its technical fabrication process and biological behavior. Considering the advantages of additive manufacturing technology, this study introduced the feasibility of developing a 3D printed resin-based composition modified with graphene nanoplatelets (GNPs) to improve properties. Acrylate-based resin was impregnated with different concentrations of GNPs (0.0-0.25wt%), and then different aspects such as mechanical, physical, biological and antimicrobial were analyzed to evaluate the effectiveness. TEM and SEM were used to characterize GNPs and their existence within the resin. Surface topography and roughness were evaluated using AFM. The degree of conversion and composition were confirmed by FTIR. Mechanical properties were detected using bending strength, microhardness and nanoindentation. Biocompatibility and antimicrobial activities were assessed with oral fibroblast and Candida albicans (C. albicans), respectively. In addition, most of the measurements were performed repeatedly after 3 months of storage in artificial saliva to evaluate performance. GNPs improved strength significantly at low concentrations ≤0.05wt%, while the addition up to 0.25wt% enhanced printed nanocomposite hardness and elasticity. The modification did not induce a toxic response, as its biocompatibility was within the recommended range of biomedical devices. Antimicrobial activity was of prominence, as GNPs showed an outstanding route of reducing C. albicans activity associated with filler proportion. The embedment of GNPs in 3D printed resin can become a key material for customized applications that require high antimicrobial, stiffness and strength properties.

Effect of Cumulative Ionizing Radiation on Flexural Strength, Flexural Modulus, and Elasticity Modulus of Dentin in Unerupted Human Third Molars

PurposeThis in vitro study aimed to investigate the changes in mechanical properties in dentin of third molars after radiation therapy using variable doses and frequencies. Methods and MaterialsRectangular cross sectioned dentin hemisections (N = 60, n = 15 per group; >7 × 4 × 1.2 mm) were prepared using extracted third molars. After cleansing and storage in artificial saliva, random distribution was performed to 2 irradiation settings, namely AB or CD (A, 30 single doses of irradiation [2 Gy each] for 6 weeks; B, control group of A; C, 3 single doses of irradiation [9 Gy each]; and D, control group of C). Various parameters (fracture strength/maximal force, flexural strength, and elasticity modulus) were assessed using a universal Testing Machine (ZwickRoell). The effect of irradiation on dentin morphology was evaluated by histology, scanning electron microscopy, and immunohistochemistry. Statistical analysis was performed using 2-way analysis of variance and paired and unpaired t tests at a significance level of 5%. ResultsSignificance could be found considering the maximal force applied to failure when the irradiated groups were compared with their control groups (A/B, P < .0001; C/D, P = .008). Flexural strength was significantly higher in the irradiated group A compared with control group B (P < .001) and for the irradiated groups A and C (P = .022) compared with each other. Cumulative radiation with low irradiation doses (30 single doses; 2 Gy) and single irradiation with high doses (3 single doses; 9 Gy) make the tooth substance more prone to fracture, lowering the maximal force. The flexural strength decreases when cumulative irradiation is applied, but not after single irradiation. The elasticity modulus showed no alteration after irradiation treatment. ConclusionsIrradiation therapy affects the prospective adhesion of dentin and the bond strength of future restorations, potentially leading to an increased risk of tooth fracture and retention loss in dental reconstructions.