Storage Duration Research Articles

Povidone-iodine in vitro antiseptic efficacy as a function of exposure duration, concentration, preparation, and length of storage.

Although 5% povidone-iodine (PVP-I) is frequently used as an ocular antiseptic agent, there is a lack of consensus regarding the effects of PVP-I concentration, storage after opening, and compounded preparation on PVP-I antisepsis. We performed a series of in-vitro experiments to determine the impact of these factors on PVP-I's inhibition of common causes of post-procedural eye infection. Inhibition of microorganism growth was measured in-vitro as a function of active PVP-I exposure time. In control experiments, PVP-I was inactivated before microorganism exposure. Tested PVP-I solutions varied in concentration (0.6%, 5%, or 10%), length of storage after opening (0, 7, or 30days), and preparation (commercial vs.compounded from stock PI solution). Tested pathogens included S. epidermidis, S. viridans, P. aeruginosa, methicillin-resistant S. aureus, methicillin-sensitive S. aureus, and C. albicans. PVP-I solutions inhibited all bacterial growth by 3min and fungal growth by 15s. Compared to 5% PVP-I, the 0.6% PVP-I was less effective in inhibiting S. viridans growth (200 ± 0 colonies vs. 7 ± 8 at 30s, P = 0.0004; 183 ± 21 vs. 0 ± 0 at 1min, P = 0.018), but more effective in inhibiting P. aeruginosa (30 ± 20 vs. 200 ± 0 at 15s, P = 0.019). Compared to commercial and newly-opened PVP-I solutions, compounded preparations and solutions stored for 7 or 30days after bottle opening either preserved or improved antiseptic efficacy against tested microorganisms. Concentration of PVP-I solution affects antiseptic efficacy within 1min of exposure, but all solutions performed equivalently at 3min. In contrast to results of prior studies investigating dilute PVP-I, the 0.6% PVP-I did not demonstrate a uniformly equivalent or superior anti-septic effect. Compounded preparation and storage length after bottle opening did not decrease PVP-I antiseptic activity.

Are edible coatings (with or without essential oil/extract) game changers for maintaining the postharvest quality of strawberries? A meta-analysis

Strawberries have limited storage potential due to their sensitive structure, resulting in high postharvest losses during transportation and storage. This meta-analysis aimed to investigate the actual effect size of utilizing edible coatings (with or without essential oils and extracts) on maintaining the quality, appearance, and sensory acceptance of strawberries during postharvest storage. The effect of different categorical moderators including the presence or absence of essential oil/extract in the coating, the polymer type (polysaccharide, protein, combination of both), storage duration (1, 2, 3, and 4 weeks), and storage temperature (room temperature and cod storage) were also determined through random-effects model analysis. The potential publication bias was examined through funnel plot (Trim and Fill method), Rosenthal’s fail-safe number and Egger’s test. After full text screening based on inclusion/exclusion criteria, a total of 56 papers, including 115 studies were selected for meta-analysis. Based on the obtained results, coated strawberries exhibited significantly (p < 0.001) higher firmness (47.3 %), total acidity (19.9 %), sensory acceptance (64.8 %), chromacity (13.3 %) and the lower weight loss (-35.6 %) and decay percentage (-46.3 %) compared to uncoated ones. Incorporating essential oils into edible coating significantly increased chromacity (27.7 %) and total acidity (21.8 %) while adding extract significantly enhanced the retaining of firmness (90.3 %) and weight (39.4 %) in stored strawberries. The results of the current meta-analysis provide better understanding and insights into the efficiency of incorporating essential oils and extracts into edible coating for fruit, particularly strawberries.

Enhancing Water Quality in Biu, Borno State, Nigeria: Impact of Storage Duration on Sachet Water

Enhancing Water Quality in Biu, Borno State, Nigeria: Impact of Storage Duration on Sachet Water

O-298 Embryo long-term storage does not affect ART outcome: analysis of 58,001 vitrified blastocysts

Abstract Study question Does duration of storage affect clinical outcome in frozen embryo transfer (FET) cycles? Summary answer Long-term storage does not affect live birth rate (LBR) in elective and non-elective FET cycles. What is known already Despite the success rates provided by the embryo vitrification programs, some studies found an association between the duration of cryo-storage and decreased clinical outcome, raising concern about the potential negative effect that prolonged storage may have on embryos. Study design, size, duration This was a retrospective multicenter study including a total of 58001 vitrified/warmed day-5 blastocysts from two different populations according to the reason for FET: elective FET which involved patients undergoing freeze all cycles (N = 16,615 blastocysts and 16,615 patients); and non-elective FET including 41,386 embryos from 25,571 patients. FETs were performed in the period between January 2010 and December 2020. Participants/materials, setting, methods Main outcome was live birth rate (LBR) and secondary outcomes were embryo survival, miscarriage, clinical and ongoing pregnancy rates. The impact of storage time and the potential confounding variables was assessed by univariate analyses. Those covariables found to significantly modify the LBR were included in a multivariable logistic regression. GEE (generalized estimated equation) was performed to allow the analysis of repeated measurements. Odds ratios (OR) and 95% confidence intervals (CI) were provided. Main results and the role of chance The blastocysts sample was clustered into deciles which provided specific storage duration categories that ranked from ≤0.67 to ≥ 4.34 and ≤1.8 to ≥ 34.81 months in freeze-all and non-elective FET, respectively. In the freeze-all group, maximum storage time was 34.8 months (2.9 years), while it was 121.2 months (10.1 years) in the non-elective FET group. Embryo survival did not show statistical differences across the categories of storage time in freeze-all and non-elective FET groups. In the freeze-all group, statistical differences were found for LBR across some, but not all the subgroups of storage duration. Similarly, in the non-elective FET group, LBR among the deciles showed statistical differences in some comparisons but not in all. However, after adjusting for confounding variables, the multivariate analysis showed no association between storage time and LBR in both groups. Blastocyst quality, body mass index (BMI), number of retrieved oocytes, endometrial preparation, male factor and uterine factor were related to the drop in the LBR in the freeze-all group (p &amp;lt; 0.05). In the non-elective FET group, the variables that showed significant association with the LBR were age at retrieval and at FET, number of embryos transferred, number of retrieved oocytes, BMI, endometrial preparation, sperm origin and female factor. Limitations, reasons for caution The retrospective design of the study represents its main limitation. Wider implications of the findings Our results demonstrate the safety of long-term cryo-storage. This is reassuring for both IVF practitioners and patients especially considering the exponential increase in FET cycles observed in the last decades. Trial registration number Not applicable

P-270 Analysis of patient response rates to automated cryomanagement platform communications by length of cryostorage and tissue type

Abstract Study question Does the length of storage time of cryopreserved samples impact patient response rate and is this influenced by the tissue type stored. Summary answer Patient response rates decline as storage time increases. The decline is faster and higher with sperm and testicular tissue, compared to oocytes and embryos What is known already Clinics are experiencing increasing inventories of cryopreserved samples. While clinics provide storage consents and contracts, an increasing issue has emerged of abandoned/unclaimed samples, even with provision of multiple disposition options. Cryomanagement is labour intensive, both in terms of physical and administrative maintenance, the latter further increasing when communication and payment from the patient inadvertently or deliberately ceases. Full cryomanagement automation including patient billing, education and engagement with disposition decisions reduces the workload for the embryologist and increases consistency. It may also provide data insights allowing clinics to identify issues and implement strategies to improve patient engagement Study design, size, duration Data were retrospectively analysed from an automated cryomanagement platform. In total, 418,895 patient accounts from 122 clinics based in the USA over the period 2015 to 2024 Participants/materials, setting, methods Data was extracted from the automated cryomanagement platform database - a live feed of clinic information – and encrypted. Logic was created to develop calculations utilising the coding language, Structured Query Language (SQL). Revenue collected was compared with collectable charges to generate the collection/response rate. Data was analysed by collection rate by tissue type to identify trends and differences Main results and the role of chance An inverse relationship was identified between storage duration and response rates. Collection rates fell from 97.44% to 68.44% in relation to oocytes, 94.87% to 45.08% for embryos, 87.01% to 29.41% for sperm, and 95.67% to 29.75% for testicular tissue. Patients with stored oocytes are least likely to abandon cryopreserved samples, with 68.44% of storage payments continuing to be received beyond 14yrs. Patients with stored sperm or testicular tissue are the most likely to abandon cryopreserved samples. Collection rates fall below 50% at 6-8yrs and 10-12yrs respectively, and beyond 14yrs storage have fallen to 29.41% and 29.75% Limitations, reasons for caution The storage duration does not necessarily reflect the duration of patient interaction with the automated cryomanagement platform, where freeze date preceeded platform implementation. Further research is necessary to identify if patients exclusively interacting with the platform from freeze date have altered response rates Wider implications of the findings Data held by automated cryomanagement systems allows accurate review and analysis of cryostorage and cryomanagement metrics. Mined data could be used to develop policies based on real-life data. Including patient treatment information and patient engagement with the system from freeze date may help identify and address reasons for abandonment Trial registration number Not Applicable

From Bed to Bench: Pre-analytical Stability of 29 Anti-infective Agents in Plasma and Whole Blood to Improve Accuracy of Therapeutic Drug Monitoring.

Therapeutic drug monitoring requires a validated assay and appropriate conditions for sample shipment and storage based on the stability of the compound to be analyzed. This study evaluated the stability of 29 antimicrobial compounds in whole blood (WB) and plasma samples under various storage conditions. The pre-analytical stability of 22 antibiotics (amoxicillin, aztreonam, cefazolin, cefepime, cefotaxime, cefoxitin, ceftazidime, ceftobiprole, ceftolozane, ceftriaxone, ciprofloxacin, clindamycin, cloxacillin, daptomycin, levofloxacin, linezolid, meropenem, metronidazole, moxifloxacin, piperacillin, sulfamethoxazole, and trimethoprim), 2 beta-lactamase inhibitors (avibactam, tazobactam), and 5 antituberculosis drugs (ethambutol, isoniazid, pyrazinamide, rifabutin, and rifampicin) was assessed by WB for up to 24 hours at room temperature (RT) and 72 hours at +4°C. The stability in plasma was evaluated for up to 6 hours at RT, 24 hours at +4°C, 1 month at -20°C, and 6 months at -80°C. Concerning WB stability, all investigated compounds were stable for 24 hours at RT, except meropenem and isoniazid, which were stable for 6 hours; however, for 24 hours at +4°C, all the compounds were stable. For storage durations of 48 and 72 hours at +4°C, all compounds were stable, except for ciprofloxacin, cotrimoxazole, and isoniazid. Concerning stability in plasma, all compounds were stable for 6 hours at RT, and all except isoniazid were stable for 24 hours at +4°C. All the tested compounds were stable for 7 days at -20°C, except isoniazid, for which a degradation of approximately 20% was observed. An important degradation was observed for beta-lactam antibiotics after 1 month at -20°C. All compounds were stable at -80°C for 6 months. The pre-analytical stabilities of several anti-infective compounds was described. The present results can be used to determine the appropriate conditions for shipping and storing samples dedicated to therapeutic drug monitoring of the investigated compounds.

Pengaruh Penyimpanan Suhu Refrigerasi terhadap Karakteristik Fisik Organoleptik, Nilai pH, Cemaran Coliform dan Salmonella spp., Yoghurt Susu Kambing dengan Penambahan Tepung Bekatul Beras Merah

Yogurt is a fermented milk product made with the help of Lactic Acid Bacteria (LAB). Goat milk yogurt with the addition of red rice bran flour contains high antioxidants, making it very beneficial for health. This study aims to evaluate the effect of storage duration on the organoleptic physical characteristics, pH value, and contamination by Coliform and Salmonella spp. in goat milk yogurt with the addition of red rice bran flour. The study used 20 samples divided into five treatment groups based on storage duration (0, 1, 2, 3, and 4 weeks) at 4 ℃, with four repetitions. Each week, organoleptic physical characteristics were tested hedonically and hedonic quality assessed by 20 semi-trained panellists, pH measured with a digital pH meter, Coliform contamination tested using the Most Probable Number (MPN) method, and Salmonella spp. contamination tested using SSA media followed by TSIA media. Data analysis for organoleptic physical characteristics was conducted using the Kruskal-Wallis test followed by the Mann-Whitney test. Data analysis for pH and Coliform contamination was conducted using one-way ANOVA followed by the Tukey test. Data analysis for Salmonella spp. contamination was done descriptively. The results showed that the storage duration significantly affected the physical characteristics of appearance, taste, and aroma (p&lt;0.05) but did not significantly affect colour (p&gt;0.05). There were very significant differences in pH values and Coliform contamination (p&lt;0.01), whereas Salmonella spp. contamination was not affected by the storage duration. The conclusion of this study is that goat milk yogurt with the addition of red rice bran flour should be consumed within three weeks based on organoleptic physical characteristics, pH value, and levels of Coliform and Salmonella spp. contamination.

Assessing kiwifruit quality in storage through machine learning

AbstractToday, diets rich in fruits and vegetables are highly recommended since they contribute to health. The high deterioration rate of fruits distinguishes them from other crops. This study aimed to evaluate the effects of various storage conditions on some quality attributes of kiwifruit through machine learning (ML) using support vector machine (SVM) models. Kiwifruits were subjected to quasi‐static loading and coated with different coatings, such as grape, date, and mulberry syrups. Then, the coated kiwifruits were stored at humidity levels of 90% and 95% in completely dark and bright environments with a Compact fluorescent lamp (CFL) bulb for 5, 10, and 15 days. Once the storage period had been completed, quality attributes of the kiwifruit, including antioxidant content, phenolic content, total soluble solids (TSS), pH, and firmness were measured. Each test was performed three times. The numerical results were analyzed through an ML approach using an SVM model on MATLAB. To predict the physical properties of kiwifruit using storage conditions and vice versa, it was found that the most accurate SVM model with a linear kernel predicted the weight loss of kiwifruit based on storage conditions, with the coefficient of determination (R2) being 0.54. To predict the biochemical properties using the storage conditions and vice versa, it was found that kiwifruit firmness was most accurately predicted by the SVM model with the Gaussian kernel, with an R2 of 0.70. Moreover, humidity and storage duration were modeled by SVMs with linear kernels, calculating the coefficients of determination to be 0.39 and 0.90, respectively. To predict biochemical properties using physical properties and vice versa, it was observed that the weight loss was more accurately predicted by an SVM with a linear kernel, with an R2‐value of 0.76. Reliable results were not obtained for further research for the other modeled parameters using an SVM approach.Practical ApplicationsIt was found that light was most accurately predicted by the linear SVM. Storage conditions revealed SVM (linear kernel) accurately predicting kiwifruit weight loss. Linear and Gaussian SVMs accurately modeled phenolic and antioxidant content, R2‐values: 0.73 and 0.34, respectively.

The Influence of High Hydrostatic Pressure on Selected Quality Features of Cold-Storage Pork Semimembranosus Muscle.

The primary objective of this investigation was to assess the influence of high hydrostatic pressure (HHP) and the duration of cold storage on the physicochemical, technological, and sensory attributes as well as the nutritional composition and shelf life of meat. The experimental framework involved utilizing samples derived from the semimembranosus muscle of pork. Each muscle obtained from the same carcass was segmented into six distinct parts, with three designated as control specimens (K) and the remaining subjected to vacuum packaging and subsequent exposure to high hydrostatic pressure (200 MPa at 20 °C for 30 min). Comprehensive laboratory analyses of the meat were conducted at 1, 7, and 10 days post slaughter. The meat was cold-stored at +3 ± 0.5 °C. The findings of the study elucidated that the application of high hydrostatic pressure exhibited a favorable impact on the extension of the raw meat's shelf life. The tests showed a significant (p < 0.05) decrease in the total number of microorganisms compared to the control sample after 7 (K: 4.09 × 105, HHP: 2.88 × 105 CFU/g) and 10 (K: 7.40 × 105, HHP: 2.42 × 105 CFU/g) days of cold storage. It was also found that using HHP increased the pH value after 1 (K: 5.54, HHP: 5.77) and 7 (K: 5.60, HHP: 5.87) days of storage.

Physicochemical characterization, sensory quality analysis, and shelf-life prediction during the storage of a total nutrient formula emulsion

The study evaluated the effects of storing total nutrient formula emulsion at 4 °C, 25 °C, and 45 °C for 6 months on its physicochemical characteristics and sensory scores. With the increase of storage temperature or prolongation of storage duration, the color of the emulsion shifted towards yellow-brown, pH and apparent viscosity gradually decreased, and vitamins A, E, and C all experienced varying degrees of degradation. Using the storage of the sample at 45 °C for 6 months as the evaluation criterion, a principal component analysis (PCA) and regression analysis were employed to establish a low-temperature storage shelf-life prediction model. The results indicated that, under storage conditions of 4 °C and 25 °C, the shelf-life of the complete nutrient formula emulsion was 18.74 and 12.19 months, respectively. The study guided the understanding of quality changes and shelf-life prediction for the total nutrient formula emulsion during storage and transportation.

Grid variability and value assessment of long-duration energy storage under rising photovoltaic penetration: Evidence from Japan

Using high-resolution grid power balance and market data, this work investigates the effects of rising solar photovoltaic generation on the variability of large-scale net grid load and spot prices, and conducts an analysis of the potential balancing profits of various grid-scale energy storage systems. Analysis results show the correlation between PV (photovoltaic) generation and electricity demand has been identified as a significant factor influencing spot price value. As PV penetration increases, the value of spot prices experiences a notable decline, with values declining to nearly zero when the share of hourly PV generation surpasses 70 %. The volatility of electricity spot prices has a substantial impact on utilization rates and economic profits of energy storage systems employed for grid energy balancing. Despite the lower roundtrip efficiency, reversible hydrogen storage system can offer positive benefits. The longer storage duration offers the advantage of capturing greater dispatch profits by exploiting price differences over extended periods. Considering the time-varying nature of spot prices, the rated power capacity of the storage system plays a significant role in determining its utilization rates. The larger rated charging and discharging ability of the storage system allows for more effective utilization of price fluctuations, resulting in greater profitability.

Validated Chromatography Method for Analysis of Formaldehyde Migration Levels in Bottled Drinking Water

Polyethylene terephthalate (PET) is utilized in the containment of potable water, nonetheless, it is susceptible to impairment due to temperature and duration of storage. Migration of formaldehyde from PET arises as a consequence of thermal deterioration. This study aims to ascertain the extent of formaldehyde transference in PET-contained drinking water of varying condition, subjected to diverse temperature setting and storage durations. An examination into the levels of formaldehyde migration in polyethylene terephthalate (PET) bottled drinking water was effectively carried out employing a validated high-performance liquid chromatography (HPLC) technique. A total of 162 water samples from three different PET bottled water brands sourced from supermarkets in Palu City, Central Sulawesi, Indonesia, underwent analysis. The formaldehyde assessment was executed utilizing a mobile phase composition of methanol:acetonitrile:water (48:12:40 v/v) for 12.97 minutes at a flow rate of 0.8 mL/minute on the 1st day, the 14th day, and the 42nd day while stored at room temperature and exposed to sunlight. Formaldehyde, lacking effective chromophoric entities and not readily ionizable, necessitated derivatization with 2,4-dinitrophenylhydrazine before HPLC analysis. The findings of the study demonstrated exceptional method linearity within the concentration span of 0.5 to 1321 µg/mL, exhibiting a commendable correlation coefficient of 0.999. The limit of detection (LOD) and quantification (LOQ) were determined to be 17.33 and 57.76 µg/mL, correspondingly. Precision evaluations displayed average intraday and interday values of 128.46±3.48% and 103.19±0.12%, respectively (%RSD &lt; 2/3 CV Horwitz ratio). The intraday and interday %recovery values were computed at 97.22±3.48% and 96.83±0.14% (n = 6). The scrutiny of formaldehyde concentrations in the water samples unveiled the absence of formaldehyde migration.

QUALITY ASSESSMENT AND STABILITY STUDIES OF METRONIDAZOLE TABLETS FORMULATIONS OBTAINED VIA CRYSTALLO CO-AGGLOMERATION TECHNIQUE

Stability studies are essential for assessing the quality, safety, and efficacy of pharmaceutical products, ensuring they maintain their properties over time. This study aimed to assess the stability of metronidazole tablets stored in a desiccator with charged silica gel following the International council for harmonization of Technical Requirements for Pharmaceuticals for Human Use guidelines. Metronidazole tablets were formulated from metronidazole co-agglomerates using different excipients by direct compression method and stored for 6 and 12 months at 25±2°C/60±5% relative humidity. Quality parameters such as weight variation, diameter, thickness, hardness, friability, content uniformity, and dissolution rate were evaluated at intervals using the United States Pharmacopeia – National Formulary and British Pharmacopeia specifications. The tablets maintained uniformity in weight, diameter, thickness and content over 12 months, meeting pharmacopeial standards. They exhibited high crushing strength, low friability, and consistent disintegration times (&lt;5 min), across formulations and storage durations with no significant changes after storage, indicating stable performance. The sustained high crushing strength, friability ratio (CSFR) and crushing strength, friability, disintegration time (CSFR/Dt) ratios suggested high tablet strength and quality. In-vitro dissolution studies showed release rates of 87.31 – 100.81 %, with a significant decrease at 6 months within pharmacopeial standards but no change at 12 months. Content uniformity was maintained throughout storage. Metronidazole tablets formulated from crystallo co-agglomerates demonstrated good stability and mechanical strength over 12 months of storage. Storage in air-tight containers with desiccants at controlled room temperature (25±2°C) or below their relative humidities is recommended for maintaining tablet quality

Advancing towards practice: A novel LC-MS/MS method for detecting retinol in dried blood spots

BackgroundTo date, clinical laboratories face challenges in quantifying retinol from DBS samples. Disputes arise throughout the whole detection process, encompassing the storage condition, the release strategy as well as the selection of internal standards. MethodsWe incubated DBS with ascorbic acid solution. Then, retinol-d4 in acetonitrile was introduced to incorporate isotopic internal standard and promote protein precipitation. Afterward, sodium carbonate solution was added to ionize cytochromes (such as bilirubin), which amplified the difference of their hydrophobicity to retinol. Subsequently, cold-induced phase separation could be facilitated to separate retinol from the impurities. In the end, the upper layer was injected for LC-MS/MS analysis. ResultsBy comparing the detected retinol content in whole blood and DBS samples prepared from the same volume, we confirmed the established pretreatment was capable to extract most of retinol from DBS (recovery >90 %). Thereafter, we verified that within DBS, retinol possessed satisfying stability without antioxidation. Indoor-light exposure and storage duration would not cause obvious degradation (<10 %). Following systematic validation, the established method well met the criteria outlined in the relevant guidelines. After comparing with detected DBS results to the paired plasma samples, 54 out of 60 met the acceptance limit for cross-validation of ±20 %. ConclusionsWe realized precise quantification of retinol from one 3.2 mm DBS disc. By circumventing conventional antioxidation, liquid-liquid/solid-phase extraction and organic solvent evaporation, the pretreatment could be completed within 15 min consuming only minimal amounts of low-toxicity chemicals (ascorbic acid, acetonitrile, and sodium carbonate). We expect this contribution holds the potential to significantly facilitate the evaluation of patients' vitamin A status by using DBS samples in the future.

Analisis Kandungan Protein pada Susu Sapi Perah Kemasan dari Peternakan Desa Gunung Perak Sinjai Barat

Humans require food and beverages that serve as building materials, regulators, and sources of energy. Protein is an essential component that functions in tissue maintenance, formation of essential body compounds, antibody production, and maintaining bodily balance. However, the protein content in packaged dairy cow milk can decrease over storage time after packaging, which may affect the nutritional value received by consumers. This study aims to identify and analyze changes in protein levels in packaged dairy cow milk over three days after packaging. The research method used is UV-VIS spectrophotometry with a Genesys 10-S spectrophotometer to measure the protein content in dairy cow milk samples taken from Gunung Perak Village, Sinjai Barat Subdistrict, Sinjai Regency. The results showed that the protein content of the milk one day after packaging was 0.027%, two days after packaging was 0.021%, and three days after packaging was 0.019%. This decrease in protein levels indicates that the duration of storage after packaging negatively affects the protein quality of the milk. The implication of these research findings is the need for special attention in the storage and distribution process of packaged dairy cow milk to maintain its protein content, ensuring that the nutritional value received by consumers remains optimal. Further research with different methods is recommended to obtain more in-depth information about the factors influencing changes in protein levels in the milk.

The Effect of Using Aqueous Extract of Cyperus Rotundus Tubers on Characteristics and Shelf Life of Chicken Nuggets

The experiment was conducted to investigate the effect of the aqueous extract of the Cyperus ‎rotundus tubers ‎on fat oxidation and stored frozen chicken nuggets' physical and microbial ‎characteristics. The extract was incorporated into minced poultry meat at concentrations of 0% (control), 0.1%, 0.2%, 0.3%, and 0.4%, and the treated samples were stored for periods of 1 and 30 days at -18°C. The addition of Cyperus rotundus extract significantly improved the pH values and water-holding capacity of the treated nuggets (T2, T3, T4, and T5) compared to the control (T1) across both storage durations. Moreover, a notable reduction in lipid oxidation markers (peroxide value (PV), thiobarbituric acid reactive substances (TBA), free fatty acids (FFA), and total volatile nitrogen (TVN) was observed in the treated samples relative to the control. Additionally, a significant decrease in the total coliform bacteria count was evident in the treated nuggets, which inversely correlated with the concentration of the Cyperus rotundus extract. The findings suggest that the aqueous extract of Cyperus rotundus tubers beneficially affects the quality and safety of chicken nuggets, enhancing their shelf life when stored at -18°C for up to 30 days‎‎‎.

Whole mount immunofluorescence analysis of fresh and stored human donor corneas highlights changes in limbal characteristics during storage

PurposeHuman donor corneas are an essential control tissue for corneal research. We utilized whole mount immunofluorescence (WM-IF) to evaluate how the storage affects the tissue integrity and putative limbal stem cells in human and porcine corneas. Moreover, we compare this information with the marker expression patterns observed in human pluripotent stem cell (hPSC)-derived LSCs. MethodsThe expression of putative LSC markers was analyzed with WM-IF and the fluorescence intensity was quantified in human donor corneas stored for 1–30 days, and in porcine corneas processed 0–6 h after euthanasia. The results were compared with the staining of human and porcine corneal cryosections and with both primary and hPSC-derived LSC cultures. ResultsWM-IF analyses emerged as a more effective method when compared to tissue sections for visualizing the expression of LSC markers within human and porcine corneas. Storage duration was a significant factor influencing the expression of LSC markers, as human tissues stored longer exhibited notable epithelial degeneration and lack of LSC markers. Porcine corneas replicated the expression patterns observed in fresh human tissue. We validated the diverse expression patterns of PAX6 in the limbal-corneal region, which aligned with findings from hPSC-LSC differentiation experiments. ConclusionsWM-IF coupled with quantification of fluorescence intensities proved to be a valuable tool for investigating LSC marker expression in both human and porcine tissues ex vivo. Prolonged storage significantly influences the expression of LSC markers, underscoring the importance of fresh human or substitute control tissue when studying limbal stem cell biology.

Prospects of nanotechnology advances in food adulterant detection, spoilage detection, packaging and preservation applications

Abstract Food is essential for the development and essential functions of our lives. Our typical dietary intake consists of vegetables, fruits, legumes, pulses, grains, and other similar foods. These ingredients can be consumed in their raw form or transformed into delicious recipes. Food adulteration refers to the deliberate introduction of potentially hazardous substances into food or food products to contaminate or adulterate them. Prolonged storage of food without sufficient preservation leads to spoilage, which poses a health risk. Food undergoes degradation over an extended duration of storage, leading to the proliferation of germs on its surface. Nanotechnology revolutionizes food safety by employing precise detection and preservation methods. Nanoparticles play a crucial role in expediting the detection of adulterants, enabling the identification of even minute amounts of contaminants like pesticides or hazardous compounds. This, in turn, ensures the preservation of the quality and safety of food items. Nanosensors are used to identify spoilage by monitoring microbial activity or changes in pH levels, to prevent the intake of spoiled food. Nano-emulsions and coatings can extend the shelf life of products by establishing protective barriers that hinder the ingress of oxygen, moisture, and microbial growth. This process effectively preserves the freshness and nutritional value of the products. In this review, we have discussed the role of nanotechnology in detecting food adulteration, food spoilage, food preservation and food packaging techniques.

Effects of the Hot Water Pre-treatments and Storage Durations on the Seed Germination of Falcata (Falcataria Falcata (L.) Greuter &amp; R. Rankin)

Seed dormancy in Falcataria falcata (L.) Greuter &amp; R. Rankin is well documented. In order to attain a higher seed germination rate, the effect of hot water pre-treatment and storage duration was determined in this study. This study found a highly significant difference (p &lt; 0.05) between the mean Cumulative Germination Percentage (CGP) between treatments. Hot water pre-treatment significantly increased the CGP of seeds. However, storage duration and its interaction with different hot water pre-treatment did not have a statistically significant effect on the CGP of seeds. Overnight (12 hrs) soaking of seeds in water with an initial temperature of 80oC yields the highest CGP regardless of the storage duration. On development, it was found that radicle emergence of imbibed seeds generally starts within 1-2 days. Bangladesh J. Bot. 53(2): 249-255, 2024 (June)

Effects of postmortem chilling times and subsequent frozen storage durations on microstructure and protein properties of tilapia (Oreochromis niloticus) fillets

Effects of postmortem chilling times and subsequent frozen storage durations on microstructure and protein properties of tilapia (Oreochromis niloticus) fillets