Stomach Wall Of Rats Research Articles

Alterations in stomach wall glycoproteins during gastric ulceration induced by pyloric ligation.

Alterations of glycoproteins prepared from the glandular (pyloric) and membranous (rumenal) parts of the stomach wall of rats have been studied polarographically, in comparison with occurance of gastr

Content of histamine and serotonin in stomach wall of rats during development of neurogenic ulceration

Content of histamine and serotonin in stomach wall of rats during development of neurogenic ulceration

A NEW METHOD FOR THE PRODUCTION OF CHRONIC GASTRIC ULCER IN RATS AND THE EFFECT OF SEVERAL DRUGS ON ITS HEALING

Several methods have been reported to produce an experimental chronic ulcer in the rat, such as thermal ulcer by Skoryna et al. (1, 2), clamping-cortisone ulcer by Umebara et al. (3), and methylcholanthrene ulcer by Lauren et al. (4). In these methods thermal and clamping-cortisone ulcers seem to be valuable tools in investigation of the healing process of gastric ulcer, because of the relatively long period of its persistence and closer histologic resemblance to human peptic ulcer. However, both methods include the undesirable procedure such as the incision of glandular portion of stomach in order to perform the thermocautery of the innersurface of stomach in the former method and the consecutive administration of cortisone acetate for 8 days after the gastric operation in the latter. On the other hand, Robert and Selye (5) have reported the method to evoke an ulcer by an injection of formalin solution into the rat stomach wall itself. We also tried the same procedure and found the gastric necrosis corresponding to the injected area. However, the necrotic tissue did not disappear from the stomach wall even 6 days after the operation so that the so-called gastric ulcer was not produced. Then we injected the acetic acid solution, which is well known to injure the gastric mucosa (6) or to evoke inflammation in rat paw in our laboratory (7), into the rat stomach wall and found the definite gastric ulcer having resemblances to the human peptic ulcer at gross and microscopical observation. This paper deals with the studies of the healing process of the produced ulcer and the usefulness for the assay method of the curative drugs of gastric ulcer.

Histamine in the pathogenesis of stress ulcers in the rat.

ARTICLESHistamine in the pathogenesis of stress ulcers in the ratRJ Levine, and EC SenayRJ Levine, and EC SenayPublished Online:01 Apr 1968https://doi.org/10.1152/ajplegacy.1968.214.4.892MoreSectionsPDF (1 MB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInWeChat Previous Back to Top Next Download PDF FiguresReferencesRelatedInformationCited ByEvaluation of gastric anti-ulcer activity in a hydro-ethanolic extract from Kielmeyera coriaceaBrazilian Archives of Biology and Technology, Vol. 48, No. 2Gastric antiulcerogenic effects ofStryphnodendron adstringens in ratsPhytotherapy Research, Vol. 13, No. 3Use of Animal Models in Peptic Ulcer DiseasePsychosomatic Medicine, Vol. 58, No. 6In vivo effects of indomethacin on the activity of metal-containing enzymesGeneral Pharmacology: The Vascular System, Vol. 23, No. 5The protective effect of meciadanol (O-methyl-3(+)-catechin) on experimental ulcerationEuropean Journal of Pharmacology, Vol. 147, No. 2Restraint stress in biomedical research: A reviewNeuroscience & Biobehavioral Reviews, Vol. 10, No. 3Gastric protection by meciadanolDigestive Diseases and Sciences, Vol. 31, No. 8Histidine decarboxylase inhibition: A novel approach towards the development of an effective and safe gastric anti-ulcer drugAgents and Actions, Vol. 15, No. 5-6The gastric antisecretory activity of 3-methoxy-5,7,3′4′-tetrahydroxyflavan (ME)—a specific histidine decarboxylase inhibitor in ratsAgents and Actions, Vol. 15, No. 3-4Effects of FPL-52694, a new mast cell stabilizer, on gastric secretion and various acute gastric lesions in ratsAgents and Actions, Vol. 14, No. 5-6Combined ulcerogenic effect of ethanol and acetylsalicylic acid on the gastric mucosa of the ratZeitschrift für Rechtsmedizin, Vol. 90, No. 4Pathogenese der akuten gastroduodenalen Läsion („Streßulcus“)The role of histamine and histamine receptors in the pathogenesis and treatment of erosive gastritisWorld Journal of Surgery, Vol. 5, No. 2Gastric anti-ulcer activity of (+)-cyanidanol-3, a histadine decarboxylase inhibitorEuropean Journal of Pharmacology, Vol. 69, No. 1Effect of histamine and stress on the gastric mucosal Ca2+ content during the development of gastric ulcersAgents and Actions, Vol. 9, No. 5-6The stress of radical pelvic surgery: A reviewGynecologic Oncology, Vol. 7, No. 1Aspirin- and indomethacin-induced ulcers and their antagonism by antihistaminesEuropean Journal of Pharmacology, Vol. 51, No. 3Is histamine a mediator in bile-induced gastric mucosal injury?Journal of Surgical Research, Vol. 24, No. 4Metabolism and Excretion of HistaminePathogenese der akuten gastroduodenalen Läsion („Streßulcus“)Vendor differences in starvation-induced gastric ulcerationPhysiology & Behavior, Vol. 19, No. 2Effect of Histamine H2 Antagonism by Metiamide on the Response of the Canine Gastric Mucosa to Acid and Bile SaltGastroenterology, Vol. 73, No. 2Effects of zinc chloride on gastric secretion and ulcer formation in pylorus-occluded ratsEuropean Journal of Pharmacology, Vol. 38, No. 2The Influence of Body Temperature on the Production of Ulcers of Restraint in the RatGastroenterology, Vol. 70, No. 5Effect of metiamide, a histamine H2-receptor antagonist, on the development of gastric stress ulcers and acid secretionEuropean Journal of Pharmacology, Vol. 36, No. 1Histamine H2 receptor: Involvement in gastric ulcerationLife Sciences, Vol. 18, No. 3Necropsy findings in fatal hypothermia casesForensic Science, Vol. 8The effects of metiamide on gastric secretion and stress ulceration in ratsEuropean Journal of Pharmacology, Vol. 33, No. 2Changes in Liver and Gastric Mucosal Hexosamine Synthesis After RestraintGastroenterology, Vol. 68, No. 2Untersuchungen zur Rolle des Plasma- und Magensafthistamins bei Patienten unter StreßbedingungenHistamine and serotonin in the gastric erosions of rats dead from exposure to cold: A histochemical and quantitative studyZeitschrift für Rechtsmedizin, Vol. 74, No. 4Metabolic contributions to gastric ulcerogenesis in micePhysiology & Behavior, Vol. 7, No. 4The stress ulcer syndromeThe American Journal of Surgery, Vol. 121, No. 6Effects of brocresine on zollinger-ellison syndrome report of 2 casesThe American Journal of Digestive Diseases, Vol. 15, No. 5Inhibition of histidine decarboxylase and diamine oxidase by 4-bromo-3-hydroxybenzyloxyamineBiochemical Pharmacology, Vol. 19, No. 5Stress UlcersNew England Journal of Medicine, Vol. 282, No. 17The Effect of Restraint and Some Ulcerogenic Agents on the Sulphation and Turnover Rates of Gastrointestinal Polysaccharides16 October 2020 | Scandinavian Journal of Gastroenterology, Vol. 5, No. 3Content of histamine and serotonin in stomach wall of rats during development of neurogenic ulcerationBulletin of Experimental Biology and Medicine, Vol. 69, No. 3Stress Ulcers — A Continuing ChallengeNew England Journal of Medicine, Vol. 282, No. 7HISTIDINE DECARBOXYLASE AND ITS INHIBITIONAnnals of the New York Academy of Sciences, Vol. 166, No. 1 Vitamin B6 inThe effect of atropine on histidine decarboxylase of the stomach of the ratPharmacological Research Communications, Vol. 1, No. 1 More from this issue > Volume 214Issue 4April 1968Pages 892-896 Copyright & PermissionsCopyright © 1968 by American Physiological Societyhttps://doi.org/10.1152/ajplegacy.1968.214.4.892PubMed5642953History Published online 1 April 1968 Published in print 1 April 1968 Metrics

Biochemical Analysis of Vagotomized Stomach Wall in Rats

The authors carried out a biochemical analysis of vagotomized rat stomach wall 2–3 weeks after a bilateral vagotomy. The biochemical extraction was performed by the Schneider-Schmidt-Thannhauser method. The phosphate fractions were measured colorimetrically according to Briggs. The nucleic acids were measured on the basis of phosphate and sugar components according to Seibert and Brown. The following compounds were determined: acid-soluble organic and inorganic phosphates, phospholipidphosphate, ribonucleic acidic phosphate, deoxyribonucleic acidic phosphate, phosphoproteid inorganic phosphate, ribonucleic acid and deoxyribonucleic acid from the whole, the glandular and the membranaceous stomach. These were compared with the some constituents of the normal rat stomach. After vagotomy a decrease was observed in acid-soluble inorganic phosphate, in organic acid-soluble phosphate, in phosphoprotein inorganic phosphate of the glandular, membranaceous and the total stomach. The phospholipid phosphate did not d...

Biochemical analysis of the stomach wall in normal rats.

The rat stomach wall was analysed biochemically. The biochemical extraction was carried out according to the Schneider, Schmidt-Thannhauser's method. Acid-soluble inorganic phosphate, acid-soluble organic phosphate, phospholipid phosphate, ribonucleic acidic phosphate, deoxyribonucleic acidic phosphate, phosphoproteid inorganic phosphate were measured colorimetrically according to Briggs's method. The nucleic acids were estimated by measuring the phosphate and sugar components. The biochemical analysis was made both from the total stomach, and also separately from glandular stomach and the rumen. It is concluded that these two parts of the rat's stomach differ biochemically.

Gnathostoma malaysiae sp. n. from Rats on Tioman Island, Malaysia (Nematoda: Gnathostomidae)

Gnathostoma malaysiae sp. n. was found in the stomach wall of rats, Rattus surifer and Rattus rattus tiomanicus trapped in a mountainous area of Tioman Island, Pahang State, Malaysia. The new species looks like Gnathostoma doloresi Tubangui, 1925, but is easily distinguished from this species by the shape of cuticular spines, the size of fertilized eggs, and the features of the caudal pedunculate papillae of the male. From the medical point of view the parasites belonging to the genus Gnathostoma Owen, 1836 are not very significant in Malaysia, although two probably indigenous cases of human gnathostomiasis were reported by Samy (1918), and Sandosham (1949). On the other hand, adult worms of the genus have been collected from some mammals in Malaysia. Gnathostoma spinigerum Owen, 1836 was reported from cats in Taiping and in Kuantan by Adams (1933), and Rohde (1962), respectively. In 1962 Miyazaki, one of the authors, and Dr. Hong-Fang Lee of the University of Singapore found three young adults of G. spinigerum in the stomach wall of one of two cats captured in the vicinity of Kuantan, Pahang (unpublished data). Moreover, Miyazaki (1960) found the eggs of this nematode in the feces of a tiger and a leopard which had been imported from Malaysia for Japanese zoological gardens. Another species of the genus reported in Malaysia is Gnathostoma doloresi Tubangui, 1925, which was found by Sandosham (1953) in a pig in Singapore. In the present paper the authors describe a new species of the genus found in the stomach wall of rats, Rattus surifer and Rattus rattus tiomanicus, trapped in a mountainous area of Pulau Tioman, an island in the South China Sea about 35 miles off the southeast coast of Pahang State, Malaysia. Received for publication 28 September 1964. * This study was supported in part by U. S. Public Health Service Grant AI 04189-02 from the ICMRT Program, Office of International Research, NIH; in part by the Office of the Surgeon General, Department of the Army. Current address of the junior author: Hooper Foundation, University of California, San Francisco. MATERIALS In April 1962 the junior author and colleagues from the Zoology Department, University of Malaya, trapped rats and other small animals at various altitudes on Tioman Island. Most of these animals were examined for helminths. Animals trapped at low altitudes-between sea level and about 1,000 ft-were all negative for Gnathostoma as follows: 6 Rattus surifer, 24 Rattus rattus tiomanicus, 3 Rattus sabanus, 1 Rattus cremoriventer, 1 Rattus exulans, 1 Callosciurus notatus, and 13 Tupaia glis. Four Rattus surifer and two Rattus rattus tiomanicus collected by Lord Medway in the forest at an altitude over 3,000 ft were also examined, and four gnathostomes-two males and two females-were found in the stomach wall of one of the four R. surifer and one female of the same gnathostome in one of the two R. r. tiomanicus. The worms were parasitic in separate outpouchings of the stomach wall, the anterior part of the body being buried with the rest of the body free in the lumen of the stomach. The infected R. surifer was trapped on Ulu Lalang Peak, and the R. r. tiomanicus was on Gunong Kajang. The worms were preserved in 70% ethanol, and the four specimens from R. surifer were sent to the senior author for identification. Gnathostoma malaysiae sp. n. (Figs. 1-4) In worms preserved in liquid, posterior third of body swells, because cuticle is loosely connected with subcuticle (Fig. 1). Headbulb with about nine rows of hooklets. Whole body covered with cuticular spines of various shapes (Fig. 2). Spines in a few rows immediately behind headbulb usually single or two-toothed (A); rapidly becoming threetoothed, measuring about 100 (u in length, with middle tooth longer than others. Part B of the body covered with this type of spines. On part C three-toothed spines gradually change into fourtoothed ones posteriorly, and part D, almost half of the slender part of the body, is covered with fouror five-toothed, and sometimes with sixtoothed spines. At beginning of swollen part of body (E) spines suddenly diminish in length, and

Quantitative effect of some steroid hormones on the mucosal mast cells and tissue eosinophils in the rat stomach wall.

ABSTRACT Prednisolone (11β,17,21-trihydroxy-pregna-1,4-diene-3,20-dione) injected into rats at 6 hour intervals for 48 hours causes degranulation of the mast cells of the gastric mucosa to an extent dependent on the dose. Tissue eosinophilia in the gastric lamina propria decreased more slowly with the smallest dose, but clearly with the higher doses. Deoxycorticosterone (21-hydroxy-pregn-4-ene-3,20-dione) in doses of varying size produced no definite degranulation of the mast cells of the gastric mucosa, nor any changes in the tissue eosinophilia of the lamina propria. It is suggested that glucocorticoids conjugate in the lamina propria of the gastro-intestinal canal with the polysaccharides of the mast granules, and that the tissue eosinophils eliminate the histamine and serotonin liberated.

Effect of Prednisolone and Deoxycorticosterone, Administered Separately and with Growth Hormone, on the Mucosal Mast Cells and Tissue Eosinophils of Rat Stomach Wall

Summary Male rats were treated with prednisolone and deoxycorticosterone acetate separately. About 5 hours after the last injection it was found that, with both substances, the mast cells in the superficial part of the gastric mucosa had been significantly reduced (degranulated), more with prednisolone than with deoxycorticosterone acetate. The tissue eosinophilia in the basal part of the lamina propria showed a very marked decrease following prednisolone, whereas no changes were caused by deoxycorticosterone acetate. Pretreatment of rats with somatotropin affected neither the capacity of prednisolone and deoxycorticosterone acetate to degranulate mast cells nor of prednisolone to produce tissue eosinophnia.

INTRA-STOMACHAL AND INTRA-INTESTINAL INOCULATIONS OF TRYPANOSOME VIRUS WITH TESTS FOR IMMUNITY

In nearly 50 per cent. of the cases, virulent trypanosome virus inoculated directly through the stomach wall of rats failed to infect these animals. The percentage of infections after inoculation of this kind seemed to vary with the virus. Surra of India proved to be the most virulent, and caderas the least. Nagana and dourine occupied intermediate positions. The rats that escaped infection showed no trace of immunity when tested with small quantities of the same virus between the twelfth and the twenty-eighth days after the intra-stomachal inoculations. The majority of the rats tested for immunity with surra of India and nagana seemed to have acquired, instead of an immunity, a certain hypersensitiveness to infection. Five of the seven nagana rats died before their controls. The results of testing the vitality of the trypanosomes of caderas, dourine, and nagana introduced directly into the stomachs and intestines of living rats seem to show that both motility and virulence are usually lost in less than two hours. Surra of India proved exceptionally resistant to the harmful influences of the stomach, for in one case, surra trypanosomes introduced into the intestines were found to be sluggishly motile in the stomach one hour and fifty-five minutes later, and in another instance, surra parasites injected into the stomach were found, when removed from that organ two hours later, to be actively motile and infectious.