Stock Suspension Research Articles

Advancing Simultaneous Extraction and Sequential Single-Particle ICP-MS Analysis for Metallic Nanoparticle Mixtures in Plant Tissues.

Engineered nanoparticles (ENPs) have been increasingly used in agricultural operations, leading to an urgent need for robust methods to analyze co-occurring ENPs in plant tissues. In response, this study advanced the simultaneous extraction of coexisting silver, cerium oxide, and copper oxide ENPs in lettuce shoots and roots using macerozyme R-10 and analyzed them by single-particle inductively coupled plasma-mass spectrometry (ICP-MS). Additionally, the standard stock suspensions of the ENPs were stabilized with citrate, and the long-term stability (up to 5 months) was examined for the first time. The method performance results displayed satisfactory accuracies and precisions and achieved low particle concentration and particle size detection limits. Significantly, the oven drying process was proved not to impact the properties of the ENPs; therefore, oven-dried lettuce tissues were used in this study, which markedly expanded the applicability of this method. This robust methodology provides a timely approach to characterize and quantify multiple coexisting ENPs in plants.

Aggregation in experimental studies with microparticles: Experimental settings change particle size distribution during exposure

The ubiquitous occurrence of microplastics is raising broad concerns and motivating effect studies. In these studies, however, particle behaviour in the water and aggregation are rarely considered leading to contradictory results reported by different studies. Using an environmentally relevant experimental setup with Daphnia magna as a test organism, we investigated how experimental conditions affect particle aggregation and the aggregate heterogeneity in terms of the particle size distribution. The experimental factors considered were (1) exposure duration (48 h vs 120 h), (2) the total mass of suspended solids (0–10 mg/l) composed of natural mineral particles (kaolin) and microplastics, (3) the proportion of the microplastics in the particle suspension (0–10% by mass), (4) dissolved organic matter (DOM; 0 vs 20 mg agarose/l), and (5) presence of the test organism (0 and 5 daphnids/vial).We found that particle aggregation occurs within the first 48 h of incubation in all treatments, no substantial change in the aggregate heterogeneity is observed afterwards. The median aggregate size was ∼2-fold higher than the nominal average particle size of clay and microplastics in the stock suspensions used to prepare the experimental mixtures. The strongest positive driver of the aggregate size and heterogeneity was DOM, followed by the presence of daphnids and the concentration of the suspended solids in the system. Also, microplastics were found to facilitate aggregation, albeit they were the weakest contributor. Moreover, besides directly increasing the aggregation, DOM relaxed the effects of the total solids and daphnids on the aggregate size. Thus, the particle size distribution was established early during the exposure and shaped by all experimental factors and their interactions. These findings improve our understanding of the processes occurring in the exposure systems when conducting effect studies with microplastics and other particulates and demonstrate the necessity to access the particle size distribution to characterise the exposure. Aslo, relevant experimental designs with microplastics must include relevant natural particulates and DOM to ensure environmentally realistic particle behaviour and adequate particle-biota interactions.

Exposure to silver and titanium dioxide nanoparticles at supra-environmental concentrations decreased sperm motility and affected spermatozoa subpopulations in gilthead seabream, Sparus aurata.

Marine pollution by nanoparticles (NPs) can be reprotoxic for fish and disturb successful reproduction of wild populations. In gilthead seabream (Sparus aurata), a mild effect on sperm motility was observed after exposure to high concentrations of silver NPs. Considering the great heterogeneity traits within a sperm sample, it is possible that NPs affect spermatozoa accordingly, modulating subpopulation profile. Thus, this work aimed to analyse NP effects in sperm motility in general and considering spermatozoa population structure, using a subpopulation approach. Seabream sperm samples from mature males were exposed for 1 h to increasing concentrations of titanium dioxide (1, 10, 100, 1000 and 10,000 μg L-1) and silver (0.25, 25 and 250 μg L-1) NPs, including Ag NP and Ag+, dissolved in a non-activating medium (0.9 % NaCl). Concentrations chosen include realistic (10-100 and 0.25 μg L-1, respectively, for TiO2 and Ag) and supra-environmental values. The mean particle diameter was determined as 19.34 ± 6.72 and 21.50 ± 8.27 nm in the stock suspension, respectively, for titanium dioxide and silver. After the ex vivo exposure, sperm motility parameters were determined using computer-assisted sperm analysis, and sperm subpopulations were later identified using a two-step cluster analysis. Results revealed a significant reduction in total motility after exposure to the 2 highest concentrations of titanium dioxide NPs, while curvilinear and straight-line velocities were not altered. Exposure to silver NPs (Ag NP and Ag+) lowered significantly total and progressive motilities at all concentrations, while curvilinear and straight-line velocities were significantly lower only at the highest concentration. Sperm subpopulations were also affected by the exposure to both titanium dioxide and silver NPs. In both cases, the highest levels of NPs triggered a decrease in the percentage of fast sperm subpopulations (38.2% in TiO2 1000 μg L-1, 34.8.% in Ag NP 250 μg L-1, and 45.0% in Ag+ 250 μg L-1 vs 53.4% in the control), while an increase on slow sperm subpopulations. A reprotoxic effect was proven for both NPs, but only at supra-environmental concentrations.

Atomic Force Microscopy Imaging of Elastin Nanofibers Self-Assembly.

Elastin is an extracellular matrix protein, providing elasticity to the organs, such as skin, blood vessels, lungs and elastic ligaments, presenting self-assembling ability to form elastic fibers. The elastin protein, as a component of elastin fibers, is one of the major proteins found in connective tissue and is responsible for the elasticity of tissues. It provides resilience to the human body, assembled as a continuous mesh of fibers that require to be deformed repetitively and reversibly. Thus, it is of great importance to investigate the development of the nanostructural surface of elastin-based biomaterials. The purpose of this research was to image the self-assembling process of elastin fiber structure under different experimental parameters such as suspension medium, elastin concentration, temperature of stock suspension and time interval after the preparation of the stock suspension. atomic force microscopy (AFM) was applied in order to investigate how different experimental parameters affected fiber development and morphology. The results demonstrated that through altering a number of experimental parameters, it was possible to affect the self-assembly procedure of elastin fibers from nanofibers and the formation of elastin nanostructured mesh consisting of naturally occurring fibers. Further clarification of the contribution of different parameters on fibril formation will enable the design and control of elastin-based nanobiomaterials with predetermined characteristics.

Preparation of Food Grade Superhydrophobic Coatings Based on Attapulgite Nanorods

The superhydrophobic coating is a promising strategy to avoid liquid foods adhesion on the packages and thus significantly reduce their waste and environmental pollution. However, food-grade superhydrophobic coatings are yet limited, especially those with excellent performances. Herein, we report the preparation of food-grade superhydrophobic coatings by the combination of attapulgite (APT) natural nanorods, stearic acid (STA), and hydroxypropyl distarch phosphate (HDP) adhesive. First, a stock suspension was prepared by an esterification reaction between APT and STA. Then, the edible HDP primer and the STA@APT suspension were sequentially spray-coated onto substrates to form the HDP/STA@APT superhydrophobic coatings. The influences of the STA and APT concentrations on the wettability and morphology of the coatings were studied. Due to the hierarchical structure and low surface energy of the coatings assembled by APT and STA, the coatings show excellent static and dynamic superhydrophobicity and self-cleaning performance. In addition, the coatings exhibit good environmental stability and excellent antiadhesive properties toward various liquid foods and are also applicable to various frequently used food packing materials. Thus, we believe that superhydrophobic coatings have a broad application prospect for food packing.

Facile Preparation of Robust Superamphiphobic Coatings on Complex Substrates via Nonsolvent-Induced Phase Separation.

Superamphiphobic surfaces have great potential in many fields but often suffer from complicated, expensive, and time-consuming preparation methods, difficulty in applying them on complex substrates, and low stability. Herein, we show a facile fabrication of robust superamphiphobic coatings on complex substrates. A stock suspension was prepared by nonsolvent-induced phase separation of a silicone-modified polyurethane (Si-PU) adhesive containing fluorinated silica (FD-silica) nanoparticles. Then, superamphiphobic surfaces could be easily fabricated via dip coating in the suspension. The influences of phase separation and Si-PU/FD-silica ratio on the wettability and morphology of the coatings were studied. The coatings feature a microscale dense and nanoscale rough texture due to phase separation and rapid solvent evaporation, which enhances the stability by forming strong linkages among the nanoparticles while achieving high superamphiphobicity by trapping air stably in the nanopores. Consequently, the coatings show excellent static/dynamic superamphiphobicity, superior impalement resistance, and good mechanical, chemical, thermal, and UV aging stability. Additionally, the coatings have good anti-icing performance as demonstrated by the greatly extended water freezing time and weakened ice adhesion force in both simulated and real conditions.

Addition of polyvinylamine in chemi-thermomechanical pulp and kraft pulp and the effects on dewatering, strength, and air permeance

The addition of polyvinylamine (PVAm) in the wet-end of the papermaking process was investigated. The changes in pulp drainage, vacuum dewatering, tensile strength, and air permeance were measured with and without PVAm. Both chemi-thermomechanical pulp (CTMP) and bleached kraft pulp were used. The dewatering and drainage abilities of the different pulps was examined by measuring the dewatering resistance (°SR), the water retention value (g/g), and the vacuum dewatering. The tensile strength and air permeance values were tested on paper sheets. The results showed that the initial dewatering was faster for pulps with PVAm, and this effect was greater for the CTMP and at higher basis weights. The strength increased when PVAm was added but only if the pulp was washed before sheet forming. The unwashed pulp with PVAm had worse formation, which resulted in lower tensile strength values. The air permeance of the sheets was higher with the addition of PVAm, primarily as a result of higher flocculation. Adding PVAm to the stock suspension in the wet-end of the paper machine has great potential for end-products that require high air permeance and tensile strength properties. PVAm could also act as a dewatering enhancing agent, but caution must be taken regarding the potential of formation problems.

Cryoprotective agents influence viral dosage and thermal stability of inhalable dry powder vaccines

Increasing viral dosage within dry powder vaccines reduces the powder mass required to elicit an immune response through pulmonary delivery. This work analyzes how cryoprotective agents affect viral activity, particle properties and thermal stability of a spray dried, inhalable vaccine vector under high viral loading. Stock suspensions of a human serotype 5 adenovirus (AdHu5) vector in either neat phosphate buffered saline (PBS), 10% glycerol in PBS, or 5% trehalose in PBS were added to a mannitol-dextran formulation prior to spray drying. At high viral loading, spray dried powder containing glycerol had a viral titre log loss of 2.8 compared to 0.7 log loss using neat PBS. Powders containing glycerol had a lower glass transition temperature (Tg) compared to all other formulations, permitting greater viral mobility and exposure to heat damage. Inclusion of glycerol also promoted particle cohesion during spray drying and lower yields. Using 5% trehalose as a cryogenic alternative, viral powders had a viral log loss of 1.5 and the highest displayed thermal stability over time. Additionally, trehalose-containing powders had smaller particles with lower water moisture content and higher powder yield compared to glycerol-containing powders. These findings demonstrate the importance of cryoprotective agent selection when developing thermostable vaccine powders.

ВЗАИМОДЕЙСТВИЕ ТОКОПРИЕМНИКА ВЫСОКОСКОРОСТНОГО ПОДВИЖНОГО СОСТАВА И КОНТАКТНОЙ ПОДВЕСКИ В РАЗЛИЧНЫХ УСЛОВИЯХ ЭКСПЛУАТАЦИИ

The paper presents a mathematical model that describes interaction of a current collector of high-speed rolling stock and catenary suspension in various conditions of operation such as rolling stock speed, current collector opening height, wear rate of current collection plates, ambient temperature, wind and glaze. The authors have determined limits of permissible operation according to quality of current collection, have established parameters of climatic and technogenic factors at which it is necessary to restrict speed on high-speed lines according to conditions of current collection. Results of the study can be used at development and production of domestic high-speed current collectors and catenary suspensions and at adaptation of import models for operation in Russia.

Antimicrobial Activity of Fuller’s Earth, Turmeric, and Sandalwood against Streptococcus Mutans, Micrococci and Coagulase Negative Staphylococci -an In vitro Study

Introduction: Sandalwood powder (Santalum alba) is used if the skin is oily for removing dark spots on the skin. Sandalwood has an anti-tanning and anti-aging property. Turmeric powder (Curcuma longa) is mainly used to rejuvenate the skin. It helps to delay aging like wrinkles and also possesses other properties like antibacterial, antiseptic, and anti-inflammatory, and many other properties. Multani mitti helps the skin in many different ways like the reduction of pore size pore sizes, removing blackheads and removing whiteheads fading freckles, soothing sunburns, cleansing skin, improving blood circulation, complexion, reducing acne and blemishes, and gives a glowing effect to your skin as they contain many-particles which can inhibit various organisms.
 Materials and Methods: In this investigation, the antimicrobial viability of Turmeric, sandalwood and Multani Mutti was tested against Streptococcus mutans, Micrococci, and coagulase-negative staphylococcus. The microorganisms were filled in strong media, and culture containing stock suspensions was made and, in this way, standard strains of Streptococcus mutans, Micrococci, and Coagulase-negative staphylococci were obtained. Microorganisms were subcultured in fitting society media to affirm their virtue.
 Results: The zone of inhibition of turmeric against Streptococcus mutans is about 11mm. The zone of inhibition of turmeric against Micrococci is about 9 mm. The zone of inhibition of turmeric against Coagulase-negative staphylococci is about 13mm. There was no zone of inhibition for both sandalwood and Multani mitti for Coagulase-negative staphylococci, Streptococcus mutans, and Micrococci.
 Conclusion: The study proves the turmeric has antimicrobial potential against Coagulase-negative staphylococci, Streptococcus mutans,, and Micrococci with good prospects of development into antimicrobial face pack or face wash

Influence of Carbon Agglomerate Formation on Micropollutants Removal in Combined PAC-Membrane Filtration Processes for Advanced Wastewater Treatment

Micropollutants (MPs) are ubiquitous in wastewater and are not effectively removed by the existing conventional treatment processes, resulting in increased environmental pollution. Nowadays, dosing of powdered activated carbon (PAC) prior to membrane filtration has emerged as an advanced wastewater treatment method for MPs removal. This study investigated the carbon agglomerate formation in the PAC stock suspension and its influence on MPs removal in PAC-capillary membrane filtration processes at both lab- and pilot-scale levels. Both lab- and pilot-scale membrane filtration results revealed that MPs removal efficiency is affected with the increase of PAC concentration in the stock suspension. For example, one of the investigated pilot tests showed a significantly reduced removal of good adsorbable MPs (from 57 to 17%) when stock suspension concentration was increased from 0.2 to 20 g/L. It is assumed that PAC agglomerates led to a slower adsorption kinetic and an inhomogeneous distribution of PAC in the membrane system. Maintaining PAC concentration in the stock suspension as low as possible (below 0.2 g/L for investigated PAC) certainly would help to avoid agglomeration problems and enhance the overall performance of the processes.

In-vitro study of lithium binding by sodium zirconium cyclosilicate (Lokelma®) or patiromer (Veltassa®)

Lithium poisoning remains common. Symptomatic lithium poisonings often require hemodialysis, especially when the patient has impaired renal function. An effective and non-invasive treatment to remove excess lithium would be desirable. We tested two recently approved, orally administered potassium binders in an in-vitro model. We used lithium-heparin tubes as both the source of lithium based prior studies showing the different volumes of serum in lithium-heparin tubes will produce apparent lithium concentrations in the range of toxicity (2.5 mmol/L and 5 mmol/L). We added three different volumes (0.5 mL, 0.75 mL, or 1.0 mL) of normal saline (NS) to the tubes. We calculated concentrations of sodium zirconium cyclosilicate (SZC, Lokelma®) and patiromer (Veltassa®) to simulate the ratio of drug to total body water for a 70 kg human. We prepared stock suspensions with different concentrations above and below the estimated ratios. We added varying concentrations of SZC or patiromer to tubes containing of NS. We measured sodium and lithium concentrations in duplicate for each concentration. Neither SZC nor patiromer reduced the lithium concentration across the range of concentrations in this in-vitro study.

Microbial Quality Assurance of Dried Roots of Chlorophytum borivilianum Sant F (Safed Musli)

Aim: To assess total bacterial load and detect E. coli and Salmonella in Chlorophytum borivilianum (Safed Musli), cultivated and processed in the Bundelkhand region.
 Study Design: Quality assurance of medicinal plant raw material is essential for the preparation of good quality herbal medicines. The dried roots of Safed Musli were collected from different regions of the Bundelkhand and total aerobic microbial count was measured.
 Methodology: We collected 10 samples of Safed Musli dried roots from different regions of Bundelkhand. A stock suspension was prepared using sterile peptone water and plated on a nutrient agar medium which was used to calculate total aerobic bacterial load as colony-forming units per gram (CFU/g). Further, biochemical tests were performed to confirm the identification of E. coli and Salmonella.
 Results: The total aerobic bacteria count in dried roots of Safed Musli was within the permissible limit (105-107CFU/g of dried raw material) as per globally recognized pharmacopoeia and other regulatory agencies such as the Central Council of Research in Ayurvedic Sciences (CCRAS), Ministry of AYUSH, Govt. of India, United States Pharmacopoeia (USP), Brazilian Pharmacopoeia (BP), European Pharmacopoeia (EP), World Health Organization (WHO), American Herbal Products Association (AHPA) and National Science Foundation/American National Standards Institute (NSF/ANSI). Although 2 out of 10 samples were found to have contamination of E. coli and Salmonella within the permissible limit of WHO, EP and NSF/ANSI.
 Conclusion: The results indicated that the Safed Musli cultivated and processed in the Bundelkhand region is suitable for the preparation of herbal medicines and food supplements.

Screening of bacteria Streptomyces Waksman and Henrici 1943 (Streptomycetaceae) Isolates from Soil Samples in Iraq

Background: The genus Streptomyces Waksman & Henrici 1943 includes aerobic, gram-positive, and filamentous bacteria which produce well developed vegetative hyphae with branches. The wall consists of a large mixture of different compounds, including peptidoglycan, teichuronic acid, teichoic, and polysaccharides. The peptidoglycan components consist of glycan as a chains of irregular N-acetyl- d-muramic acid (NAM), diaminopimelic acid, and N-acetyl-d-glucosamine (NAG) and DAP, which is unique in the cell walls of prokaryotic microorganisms. The teichoic and teichuronic acid are chemically bonded to peptidoglycan. Methods: One gram of soil samples was used to make suspension, by adding 99 mL of sterile distilled water (stock suspension) into it and shaking it in a shaker at 160 rpm for 30 minutes at room temperature. Serial dilutions from 0.1-0.001 were made from the stock suspension, and left for 10 minutes. After shaking, 0.1 mL of each dilution was cultured on Yeast Extract and Malt Extract agar (YEME) with Streptomycin 50 ug/mL. The inoculated plates were incubated at 28 °C for 7 to 10 days. Based on cultural characteristics, suspected colonies of Streptomyces were selected, which are characterized as small, white, pin-point, rough, chalky, and a clear zone of inhibition around them. These colonies were confirmed their identification by types of Gram’s stain, aerial and substrate mycelium color, pigment production, and pigment color. Streptomyces were re-streaked on International Streptomyces project (ISP) to obtain pure colonies used for identification. Results: The current study aimed to screen bacteria Streptomyces isolates. Only 21 samples of soil were suspected to contain Streptomyces, and 45 isolates were obtained with different morphology types per samples of soil. The colonies suspects were selected basis on color that ranged from gray, white and creamy. The microscopic examination of local Streptomyces spp. after Gram-staining method was conducted. The observations revealed that local Streptomyces is gram positive and rod shaped similar to features of fungal in possessing branched mycelium. The Streptomyces produced extra cellular enzymes like amylase, urease, catalase, protease, Gelatinase, cellulase and phosphatase. Utilization of citrate was positive, with no Melanine reaction production and soluble pigmented, and negative for indole production. Conclusion: The identification of the Streptomyces is a very complex process. Morphological and biochemical characteristics are two important aspects for the classification in the Streptomycetaceae family. By studying the morphological, cultural, and biochemical characteristics, it is observed that the local isolates are belonging to the genus of Streptomyces.

Isolation and Use of Bacillus thuringiensis for the Production of Bio-Insecticide in Control of Mosquito Larvae

Bacillus thuringiensis is a widely studied bacterium and it is known for its use in pest management. It is selectively active on pests and less likely to cause resistance; hence it is considered a suitable replacement to chemical pesticides. The study assessed the potential of Bacillus thuringiensis in controlling mosquito larvae. Bacillus thuringiensis isolates selected were tested against secondary stage larvae of mosquito. Thirty-six larvae (6 each) were transferred into each test tubes (7 x 9) cm with 30ml sterile distilled water. The stock suspension of cultures of Bacillus thuringiensis in broth was diluted to 107, 106, 10 5, 104, 103 and 102 in sterile water, following the McFarland standard method for microbial load count. The test tubes were kept at room temperature, larval mortality was observed over time within 24hrs. The results showed that all mosquito larvae died at the 107 and 106 dilutions but at dilutions 105, 104 and 103 though affecting mosquito larvae, it was highly dependent on time because there was a decrease in concentration. The study showed that B. thuringiensis is safe for use in aquatic environments, including drinking-water reservoirs, for the control of mosquito, black fly and nuisance insect larvae. The products should contain the ICPs and be free from other microorganisms and biologically active metabolites.

Assessment of Biolog EcoplateTM method for functional metabolic diversity of aerotolerant pig fecal microbiota

In the last decades, gut microbiota and its role in mammal host development and health have been increasingly investigated. Metabolites produced by gut microbiota can affect intestinal homeostasis and immune system maturity and activation, and in turn, they can influence the health and growth performance of livestock. Therefore, a better understanding of the functional metabolic capability of the gut microbiota would be appreciated by the scientific community. In this study, the BiologTM Ecoplates technology was applied for studying the metabolic potential of the aerotolerant microbial community of pig fecal samples, evaluating the interference of different storage conditions and cell concentrations. The length of time for which a fecal sample maintained detectable and unchanged microbial metabolic activity was also investigated. Two assays aimed to evaluate differences in the metabolic activities between fresh and snap-frozen fecal samples at different dilutions and at different lengths of times of preservation at −80°C were carried out. The biodiversity and the predicted functionality of the entire bacterial community through a targeted metagenomic approach were also explored. The results highlighted that snap freezing of fecal samples preserved the metabolic activity of the microbial community when compared to fresh feces. Sample storage at −80 °C did not significantly affect the metabolic activity of the microbial community, which was stable for 150 days. Furthermore, the highest metabolic activity was detected with 1:2 to 1:5 dilutions of the stock suspension. BiologTM Ecoplates technology is a rapid and useful method to explore microbial communities’ metabolism in animal fecal samples contributing to investigate host animal physiology.Key points• Freezing of samples can preserve the functional activity of the aerotolerant microbial community for 150 days.• The concentration of microbial cells strongly influences metabolic activity detection.• Sequencing coupled with the BiologTMEcoplates could be a strategy to evaluate the metabolic potential of the microbiota of the fecal sample.Graphical abstract

Microalgae cultivation in wastewater effluent from tilapia culture pond for enhanced bioethanol production.

The large number of wastewaters are generated because of the various production processes. Vegetable and fish processing can be considered an important industry for wastewater generation. The essential method for completing this waste is to digest the organic matter using anaerobic digestion followed by aerobic wastewater treatment processes; however, wastewater from tilapia culture pond retains considerable quantities of inorganic substances, particularly nutrients like nitrogen and phosphorus. The optimal conditions for cultivating Chlorella vulgaris from wastewater treatment effluent from tilapia culture pond were investigated in this study. The appropriate conditions were found to be 10% initial stock suspension, 20 cm depth, and 12 days of culture conditions. C. vulgaris had an optical density of 0.649, a cell density of 17.68 × 105 cells/mL, and biomass of 0.376 ± 94.21 mg/L after cultivation. Discharged wastewater from the fishpond was utilized for the improved growth of microalgae and obtained biomass was used for bioethanol production. This study verified that fishpond wastewater is the best source of nutrients for algal mass production and biofuel applications.

Circulating Dickkof-1 as a potential biomarker associated with the prognosis of patients with rheumatoid arthritis-associated interstitial lung disease.

Circulating Dickkof-1 as a potential biomarker associated with the prognosis of patients with rheumatoid arthritis-associated interstitial lung disease.

Antimicrobial activity of the combination (Nano-Bio) of Artemisia absinthium with copper nanoparticles

Nanoparticles show the lowest level of toxicity in the life cycle and ecosystem. Therefore, using these substances to combat pathogens may be an appropriate choice. Artemisia absinthium is traditionally used as an anthelmintic, antiseptic, antispasmodic, and worldwide for bacillus dysentery. The oil composition was analyzed by gas chromatography: mass spectrometry. Then, a nano-bio formulation was formed by inoculating a copper nanoparticle (50, 100 μg / ml) into the plant oil extract. Microorganisms were provided by Atatürk University Research Hospital. Examples were Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus epidermidis, Acinetobacter baumannii, Staphylococcus aureus, Enterobacter aerugenes, Candida albicans, and E.coli. Minimum inhibitory concentration values were determined for Pseudomonas aeruginosa (MIC = 500 μg / ml), Klebsiella pneumoniae (MIC = 500 μg / ml), Proteus mirabilis (MIC = 500 μg / ml). Staphylococcus epidermidis (MIC < 1.95 μg / ml), Acinetobacter baumannii (MIC = 500 μg / ml), Staphylococcus aureus (MIC < 125 μg / ml), Enterobacter aerugenes (MIC = 500 μg / ml), E.coli (MIC = 500 μg / ml), Candida albicans (MIC = 500 μg / ml), respectively. Anti-microbial activity of camphor extract of essential oil was tested by disk diffusion method. Plant extracts should be considered when used in part of the substitution process. A stock suspension was prepared by resuspending the nanoparticles in double distilled water to obtain a final concentration. And artemisia mixed CuO nanoparticle was determined by applying standard bacteriological methods with agar dilution method against 9 microorganisms isolated. Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus epidermidis, Acinetobacter baumannii, Staphylococcus aureus, Enterobacter aerugenes, Candida albicans, and E.coli. Values (μg / ml) were 50, 100 copper. Their use as antimicrobial compounds to combat a large number of resistant pathogens is promising for alternative therapy.

NanoBio challenge: Investigation of antimicrobial effect by combining ZnO nanoparticles with plant extract Eleagnus angustifolia

Eleagnus angustifolia has antioxidant, anti-inflammatory, analgesic, anti-cancer, Anti- microbial activity and scar healing properties. Zinc oxide (ZnO) has got much attention in the recently because it is stable under diverse environmental conditions, and fabrication at low temperature. ZnO NPs are believed to be nontoxic, bio-safe, and biocompatible. Recently, in many studies ZnO particles shown antimicrobial activity against both Gram-positive Gram-negative bacteria and even antibacterial activity against spores In the light of information, The aim of this study was to investigate the antimicrobial activity of plant and ZnONPs. In our study, aqueous ethanol extracts of E. angustifolia were prepared and the antimicrobial activity against some pathogenic microorganisms. The MIC was taken as the lowest concentration that inhibited growth after incubation. The test was performed at final concentrations of each extract 12.500;6.250; 3.125; 1.562;781; 390;195;97;48.8; 24.4 µg/mL for microdilution. ZnO. A stock suspension was prepared by resuspending the nanoparticles in double-distilled water to yield a final concentration. Serial 2-fold dilutions of nanoparticles ranging from 0.00625 to 1.6 mg/ml were prepared in a 96-well microtiter plate using MH broth. The MIC values of E. angustifolia ethanol extract for. P. mirabilis, C. albicans, E. faecalis, S. aureus, S. epidermidis, E. coli and P. aeruginosa, A. baumannii were eth were ≥ 12.500, 1.562, 1.562, 3.125, 1.562, ≥ 12.500, ≥ 12.500, ≥ 12.500 µg/ml, respectively. ZnONPs., ≥ 1.6, 0.05, 0.2, 0.05, 1.6, ≥ 1.6. According to the results in our study, the highest effect was observed on gram positive bacteries and yeast. Result of this study suggests that E. angustifolia extracts can be effective in all tested microorganisms. And ZnoNPs effected Gram positive bacteria. Further studies in the effect of different dosages and duration are suggested. Future studies should elucidate the components responsible for antimicrobial activity of these extracts against target cultures.