Stimulatory Effect Research Articles

Stimulative effect of chitosan with amino acid to enhance growth, essential oil, and some physiochemical characteristics of two Mentha cultivars

Background Mint plants (Mentha spp.) are a member of the Lamiaceae family and it has long been used in medicine. Its applications include carminative, anti-inflammatory, antispasmodic, antiemetic, diaphoretic, analgesic, stimulant, emmenagogue, and anticatarrhal. Adding chitosan to medicinal plants has a major role in the process of secondary metabolism, as its addition stimulates the production of chemical compounds or essential oils in the plant. Amino acids are one of the possible strategies for increasing agricultural productivity. They are organic nitrogen polymers that are used as the building blocks of proteins and enzymes. Objective This study aimed to determine how chitosan, in combination with or without the foliar application of amino acids, affected the growth and physiological traits of two cultivars of mint. Materials and methods Two pot trail investigation studies were carried out during the two consecutive seasons 2021 and 2022 under the natural conditions of the greenhouse of the National Research Center (NRC), Dokki, Giza, Egypt. To study the effect of two levels of chitosan (1.5 and 3.0 g/l with amino acid at rates of 50 and 100 mg/l) as foliar application on growth, essential oil, and some physiochemical characteristics of two Mentha (Mentha viridis and Mentha longifolia L.) cultivars. Results and conclusion The results show significant differences between two mint cultivars in the growth parameters of mint plants. Plants of M. viridis variety were characterized by the highest significant values of herb fresh weight, number of branches/plants, essential oil (%), flavonoid content, and protein %, while the M. longifolia variety was superior in plant height, herb dry weight, chlorophyll a, chlorophyll b, carotenoids, total pigments, indole acetic acid, phenol, carbohydrates %, free amino acids, flavonoid, and proline contents. Using chitosan as foliar treatments at different concentrations with or without amino acid significantly increased all studied traits. The interaction between two cultivars and foliar treatments of high rates of chitosan and amino acid gave the maximum significant increase of plant height, photosynthetic pigments and indole acetic acid, phenol, protein, free amino acid, and proline contents as well as antioxidant activities (DPPH%).

Digital transformation and technology innovation: evidence from Chinese manufacturing listed enterprises

ABSTRACT The listed A-share manufacturing enterprises in China from 2010 to 2019 serve as the research samples in this study. Moreover, the influence mechanism and effect of digital transformation of enterprises on technology innovation are studied at the micro level. The digital transformation of enterprises can facilitate technology innovation, and this effect continues to be significant after a series of endogenous and robustness tests are performed, as indicated by the results of this study. For mechanism, digital transformation is capable of indirectly affecting technology innovation via three channels, including reducing costs, elevating the efficiency of human capital, and deepening R&D collaboration among enterprises. In-depth analysis suggests that the effect of digital transformation on technology innovation exhibits certain heterogeneity in the development level of regional digital economy and the capital intensity of enterprises. Furthermore, enterprises in areas with higher development level of digital economy and those exhibiting higher capital intensity better enjoy the stimulating effect of digital transformation on technology innovation.

Fulvic and Salicylic Acids Improve Morpho-Physio-Biochemical Attributes, Yield and Fruit Quality of Two Mango Cultivars Exposed to Dual Salinity and Heat Stress Conditions

Abstract Purpose The present research’s main objective was to explore the potential stimulative effect of fulvic acid (FA) as a soil conditioner and salicylic acid (SA) as foliar spraying on morpho-physio-biochemical attributes, fruit yield, and quality of ‘Sediek’ and ‘Ewais’ mango cultivars (cvs.) grown under saline calcareous soil and heat stress conditions Methods Eight treatments, namely, 200 (FA200) and 400 (FA400) g FA tree−1 as a soil addition, 200 (SA200) or 400 (SA400) mg SA L−1 as foliar spraying, and their bilateral combinations (e.g., FA200 + SA200, FA200 + SA400, FA400 + SA200, and FA400 + SA400) compared to untreated control on morpho-physio-biochemical attributes, yield, and fruit quality of ‘Sediek’ and ‘Ewais’ mango cvs. grown under saline calcareous soil and heat stress conditions were assessed. These treatments were carried out using a split-plot arrangement in a randomized complete block design replicated three times during the 2022 and 2023 seasons. Results Cultivar Sediek had higher SPAD value, leaf proline and phenolic content, leaf area, fruit weight, yield and vitamin C than Ewais which had higher tree water status and shoot length. Saline calcareous and heat-stressed mango trees without FA or/and SA treatment had a significant decline in their water status, photosynthetic traits, biochemical responses that adversely affected growth and yield and fruit quality. However, soil FA addition and foliar SA spraying alone or in combination at any application level attenuated the negative effects of saline calcareous and heat-stress conditions via enhancing the morpho-physio-biochemical attributes, positively reflecting in tree growth, yield, and fruit quality. FA treatment alone improved tree water status (membrane stability index; MSI and relative water content; RWC), photosynthetic traits (SPAD value and maximum photochemical efficiency; Fv/Fm), leaf area, fruit weight, fruit yield, and fruit vitamin C (fruit vit.C), while SA treatment alone enhanced leaf proline and phenolic content, shoot length, and fruit total soluble solids (fruit TSS) compared to the non-FA or SA-treated control. Moreover, co-application of FA400 and SA400 effectively alleviated the harmful impacts of dual stress of heat and salinity on mango trees by improving MSI, RWC, SPAD value, Fv/Fm, proline and phenolic content, fruit weight, fruit yield, fruit TSS, fruit vit.C by 19.7, 26.1, 46.7, 18.7, 101.7, 390.7, 42.7, 6.8, 22.6, 69.6% (averages of the two seasons), respectively, compared to the non-FA or SA-treated trees. Conclusion It is recommended to add 400 g FA tree−1 to soil integrated with 400 mg SA L−1 foliar spraying four times at 30-day intervals for improving water status and photosynthetic traits, proline and phenolic accumulation, thereby growth, yield, and fruit quality of mango trees exposed to dual stress of heat and salinity under arid conditions.

How Traditional Fishing Villages Move Towards Sustainable Management: A Case Study of Industrial Transformation and Multi-Party Governance Models

The purpose of this study is to explore the key roles and functions of institutions or organizations in the process of the spatial activation and industrial transformation of leisure fishing villages and to summarize this operating model. This study takes Xunpu Village, Quanzhou City, Fujian Province as the research object, which is well-known for its recreational fisheries. It mainly uses case study methods and uses on-site surveys and interviews to understand the formation time, spatial distribution, and business type information of the Zanhuawei shops in Xunpu Village. Zanhuawei, which refers to the traditional hairstyle of Xunpu women, was later applied for national intangible cultural heritage status in China. After the outbreak, the spread of Zanhuawei on the Internet inspired a large number of tourists to visit Xunpu Village to experience Zanhuawei. The study found that (1) Zanhuawei industry replaced traditional fishery and became the leading industry type in Xunpu Village. (2) The operating model led by the government and integrating public and private funds has had a stimulating effect on the revitalization of local commercial and residential space, and a large number of idle spaces have been reactivated. (3) The cooperation model between a large number of investors and local fisherwomen has completely changed the economic model of the village. Zanhuawei industry mobilized all official and media resources to promote spatial activation at various scales, including regions, settlements and commercial spaces.

SO2 activates Th17 cells through the JAK1,2/STAT3 signaling pathway

ObjectiveTo investigate the effect of SO2 on Th1/Th2/Th17 cells in allergic rhinitis (AR) and the role of JAK1, 2/STAT3 signaling pathways.To Provide potential directions for the treatment of AR. MethodsFifteen AR patients were enrolled as the experimental group, while 15 healthy volunteers served as the normal control group. After collecting venous blood, peripheral blood mononuclear cells (PBMCs) were isolated and cultured, followed by the addition of SO2 derivatives and the JAK inhibitor Ruxolitinib. Flow cytometry was employed to assess alterations in the Th1/Th2 and Th17/Treg cell balance upon stimulation with SO2 and Ruxolitinib. qRT-PCR was utilized to detect the expression of Th1-related cytokines IL-2 and IFN-γ, Th2-related cytokines IL-4 and IL-5, Th17-related cytokines IL-17A and RORγt, as well as genes JAK1, JAK2, and STAT3. Flow cytometric cytokine analysis was conducted for quantitative assessment of the expression levels of inflammation-related cytokines in PBMC culture supernatants after stimulation. In addition, we stimulated the Jurkat T lymphocyte cell line with SO2 derivatives, added Ruxolitinib as an inhibitor, and used Western blot analysis to further determine the effects of SO2 on Th cells and the role of the JAK1,2/STAT3 signaling pathway in this process. ResultsStimulation with SO2 derivatives upregulated the expression levels of Th2 cells and associated cytokines, as well as Th1 cells and associated cytokines. both AR patients and healthy individuals displayed increased percentages of Th17 cells and Th17/Treg ratios in PBMCs. The expression of IL-17A, RORγt, and IL-6 was also elevated. Under SO2 stimulation, the expression of JAK1, JAK2, STAT3, and RORγt in Jurkat cells increased. Moreover, after the application of Ruxolitinib, the JAK/STAT signaling pathway was inhibited. This led to a reduction in Th17 cells and IL-17A levels in both AR patients and healthy individuals, as well as a decrease in RORγt expression in Jurkat cells. Additionally, the expression of IL-5 decreased in healthy individuals. ConclusionSO2 exposure exacerbated Th1/Th2/Th17 inflammation in AR patients and induced Th1 and Th17 inflammation in healthy individuals. The stimulatory effect of SO2 on Th17 cell differentiation could be inhibited by Ruxolitinib. This suggests that the Th17 inflammation induced by SO2 stimulation may be related to the activation of the JAK/STAT signaling pathway, and this has been confirmed in the Jurkat cell line.

The crucial role of NR2A mediating the activation of satellite glial cells in the trigeminal ganglion contributes to orofacial inflammatory pain during TMJ inflammation

Temporomandibular joint inflammatory diseases are a significant subtype of temporomandibular disorders (TMD) characterized by inflammatory pain in the orofacial area. The N-methyl-D-aspartate receptor (NMDAR), specifically the NR2A subtype, was crucial in neuropathic pain. However, the exact role of NR2A in inflammatory pain in the TMJ and the molecular and cellular mechanisms mediating peripheral sensitization in the trigeminal ganglion (TG) remain unclear. This study utilized male and female mice to induce the TMJOA model by injecting Complete Freund's adjuvant (CFA) into the TMJ and achieve conditional knockout (CKO) of NR2A in the TG using Cre/Loxp technology. The Von-Frey filament test results showed that CFA-induced orofacial pain with reduced mechanical withdrawal threshold (MWT), which was not developed in NR2A CKO mice. Additionally, the up-regulation of interleukin (IL)-1β, IL-6, and nerve growth factor (NGF) in the TG induced by CFA did not occur by NR2A deficiency. In vitro, NMDA activated satellite glial cells (SGCs) with high expression of glial fibrillary acidic protein (GFAP), and both NMDA and LPS led to increased IL-1β, IL-6, and NGF in SGCs. NR2A deficiency reduced these stimulating effects of NMDA and LPS. The regulation of IL-1β involved the p38, Protein Kinase A (PKA), and Protein Kinase C (PKC) pathways, while IL-6 signaling relied on PKA and PKC pathways. NGF regulation was primarily through the p38 pathway. This study highlighted NR2A's crucial role in the TG peripheral sensitization during TMJ inflammation by mediating ILs and NGF, suggesting potential targets for orofacial inflammatory pain management.

Allelopathic effects of leaves extract of parthenium hysterophorus l. On seed germination of three Rice varieties

This study investigates the allelopathic effects of Parthenium hysterophorus L. leaf and root extracts on the germination and growth of three rice varieties (Hira dhan, BRRI dhan-28, and BRRI dhan-29) in Bangladesh. Leaf and root extracts of P. hysterophorus were prepared at different concentrations and applied to rice seeds in a controlled environment. Results showed that low concentrations of the extracts inhibited seed germination and growth, with significant differences among rice varieties (p < 0.05). Hira dhan exhibited the most sensitivity, with germination rates as low as 42.86% under high extract concentrations. In contrast, BRRI dhan-29 showed a stimulatory effect, particularly at low concentrations. The shoot and root lengths of Hira dhan and BRRI dhan-29 were significantly inhibited at higher concentrations, while BRRI dhan-28 demonstrated growth stimulation. The fresh and dry weights of roots and shoots varied significantly among the varieties with the greatest inhibitory effect observed in BRRI dhan-29. These findings align with previous studies, suggesting that allelochemicals in P. hysterophorus, such as parthenin and phenolic acids, play a critical role in inhibiting rice growth. The study emphasizes the need for further research on allelopathic interactions and suggests that controlling P. hysterophorus at the crop emergence stage could mitigate its suppressive effects on rice growth. Bangladesh J. Bot. 53(3): 411-421, 2024 (September)

THE EFFECTIVENESS OF USING SUBSIDIES FOR CERTIFIED SEED MATERIAL USED FOR SOWING OR PLANTING

The work aims to assess the effectiveness of the subsidy system for purchasing certified seed material. The assessment was made based on data from the Statistics Poland on the sown area, data from the State Plant Protection Inspectorate on seed trade, and data from the Agency for Restructuring and Modernization of Agriculture on the amount of support provided to farmers, the analyzed changes in the amount of subsidies and the supply of certified seeds. Analyzing the motivation of farmers buying seeds was based on surveys conducted on farms in 2023 – the research concerned species covered by the subsidy system for cereals, potatoes, and large-seeded legumes. In total, the results were collected from 1,008 farms. A stimulating effect of subsidies in the initial period of their application and an improvement in the supply of certified seed material to farms was demonstrated. Maintaining the subsidies unchanged, and later, even at a nominally lower level, resulted in the disappearance of their stimulating effect. The main factors limiting farmers’ motivation to purchase certified seed material were indicated: the high prices of seed material, a lack of knowledge about the benefits of using certified seed material, low subsidy amounts, the exhaustion of the de minimis limit, and thus the possibility of using subsidies as well as an overly complicated subsidy system.

Estimation of the impact of investment in fixed assets and employment on GDP using the Cobb-Douglas function

The main objective of the study is to determine the degree of influence of labor and investment in fixed capital on the total volume of output in Kazakhstan. The paper uses the multiple regression analysis method based on the Cobb-Douglas production function. The results of the empirical analysis showed that with a change in investment in fixed capital by 1%, the volume of production will increase by an average of 0.747%. Accordingly, with a change in the number of employed people by 1%, the volume of production will increase by an average of 2.589%. The study revealed a low contribution of technological progress to the development of the manufacturing industry. Based on the analysis, a conclusion was made about the presence of increasing returns to the scale of production, since the sum of the elasticity coefficients of production for capital and labor is greater than one. The high rate of contribution to labor costs (2.589%) indicates that the growth of labor intensity in the manufacturing industry of Kazakhstan has a stimulating effect on the economic growth of production, and also brings a positive economic effect to production.

The Impact of ESG Rating Events on Corporate Green Technology Innovation under Sustainable Development: Perspectives Based on Informal Environmental Regulation of Social Systems

Corporate green technology innovation is an important driver to promote the green transformation of the manufacturing industry 4.0 and an important engine to achieve China’s carbon peak, carbon neutrality, and high-quality economic development. Based on the theory of informal environmental regulation of social systems, this study empirically analyzes the impact of ESG rating events on corporate green technology innovation by constructing a multi-period DID model using panel data of Chinese listed companies from 2010 to 2022 as the research sample. The findings suggest that ESG rating events and ESG scores can stimulate corporate green technology innovation. ESG rating uncertainty can inhibit corporate green technology innovation. The mediation effect analysis shows that ESG rating events can stimulate firms’ green technology innovation by reducing financing constraints, increasing the degree of corporate internal control, and increasing R&D investment. In addition, heterogeneity analyses indicate that ESG rating events have a better stimulating effect on the quantity of innovation of non-state-owned enterprises than state-owned enterprises, while the stimulating effect on the quality of innovation of non-state-owned enterprises is inferior to that of state-owned enterprises. Moreover, the innovation stimulation effect of ESG rating events shows a trend of east-high and west-low in geographical distribution. Therefore, the government should accelerate the construction of the ESG rating system, promote the degree of convergence with international standards, and improve the level of enterprises’ utilization of digital technology while paying attention to informal environmental regulation.

Red-light photons on skin cells and the mechanism of photobiomodulation

Red light (600–700 nm, ∼2.1–1.8 eV) consists of a low-energy radiation with a high capacity to penetrate the skin and to induce stimulatory effects. These characteristics make this wavelength range very promising for light-based therapies. Aiming to discuss the mechanisms of action of photobiomodulation, we start by providing a broad perspective of the skin and of its interaction with light, focusing on the endogenous photosensitizers, on the formation of excited states and reactive oxidants, and on the activation of signaling effectors. The peculiar aspect of the photons in the red spectral range is that they are much less absorbed by the endogenous photosensitizers and consequently generate a lot fewer reactive oxidants (when compared with the other ranges of visible light), allowing the skin to mainly experience the consequences of several signaling pathways that are activated during the skin interaction with red-light photons. Indeed, the effects of red light in epithelial cells involve the control of metabolic responses, the modulation of several key genes and transcriptions factors, as well as the regulation of the intracellular nitric oxide stocks. In this article, we discuss how red light interacts with all these variables and end up causing a vigorous tissue activation. We also analyzed the effect of red-light photons on the nitric oxide homeostasis, with implications for the phototherapy of psoriasis. It is likely that several of the observations and mechanisms described for the interactions of red-light photons may also be occurring during and after the interaction with other photons with similar energy.

Enhanced fig tree production through endomycorrhizal fungi inoculation: A nursery study on tree cuttings derived from the Ifrane Region, Morocco

The mycorrhizal association represents a crucial symbiotic bond necessary for the vitality of the majority of plants. This report aims to investigate the impact of endomycorrhizal fungi on the production of fig trees from cuttings. The research took place in a nursery environment by inoculating fig cuttings sourced from three sites in Morocco with a composite inoculum of arbuscular mycorrhizal fungi (AMF). The mean root and vegetative masses of the inoculated plants after 140 days were 33.1 g and 35 g compared with 1.8 g and 6.4 g in the control. The mean shoot length and number of leaves developed from treated cutting were 98.5 cm and 17, respectively 29 cm and 4 leaves in control. Thus, the newly formed roots showed a well-established mycorrhizal colonization and contained different structures characteristic of arbuscular endomycorrhizae: arbuscules, vesicles, endophytes, hyphae and spores. The frequency and intensity of root mycorrhization were approximately 80% and 15.5%, respectively. The contents of arbuscules and vesicles were 2.5% and 5.5%. The study of formed spores showed a combination of 52 spores per 100 g of soil, belonging to 11 species and 3 genera: Glomus, Acaulospora and Rhizophagus with dominance of the genus Glomus (85%). Glomus macrocarpum and Glomus deserticola were the most abundant species, appearing at 30.77% and 19.23%, respectively. The results demonstrate a pronounced stimulating effect on both root formation and vegetative shoot development in the inoculated cuttings. Specifically, the early inoculation of fig cutting with the AMF inoculum significantly enhanced rhizogenesis end root system development. Consequently, the gradual establishment of mycorrhizal symbiosis at the root level of inoculated plants led to good development of the root mass and the vegetative mass. The inoculated plants showed good root and vegetative mass development due to the installation and the progressive development of mycorrhizal symbiosis at their roots.

Биотестирование меламина с использованием многокомпонентной тест-системы

In recent decades, halogen-containing flame retardants have been gradually replaced by safer flame retardants, including nitrogen-containing compounds. However, their ecotoxicity is poorly studied. The ecotoxicity of melamine, a widely used nitrogen flame retardant, at concentrations equal to MPC in water (4 mg/L), 5 MPC (20 mg/L) and 10 MPC (40 mg/L) was assessed in this study using a complex of test organisms. The assessment of ecotoxicity using the dehydrogenase activity of Dietzia maris AM3 as a test microorganism revealed a stimulating effect of melamine at a concentration equivalent to the MPC; concentrations of 5 and 10 MPC inhibited the activity of dehydrogenases by 19.3% and 10.5%, respectively, indicating low toxicity. No toxic effects were observed on protococcal algae Chlorella vulgaris Beijer at any of the tested concentrations. The effect of melamine solutions on the mortality of crustaceans Daphnia magna Straus was noted only at a concentration of 10 MPC, and the mortality rate was 25%. Lemna growth inhibition test showed that melamine at concentrations equal to the MPC and 5 MPC had an inhibitory effect on the growth of Lemna minor L. It also caused a significant decrease (by 47–52%) in the total chlorophyll content in fronds when exposed to all studied concentrations of melamine.

The impact of plant cryoextract on productivity and factors of innate immunity of pond fish against the background of stress in the experiment

The search for effective and environmentally friendly means to increase the productivity and natural resistance of animals, especially in aquaculture, remains an urgent socio-economic task. This article presents the results of the study of the effect of the cryobiological supplement ‘Immunolife-Fish’, based on medicinal plants, on the weight gain of juvenile common carp (Cyprinus carpio) and indicators of innate immunity of two-year-old common carp (Cyprinus carpio) and silver carp (Hypophthalmichthys molitrix) under the influence of thermal stress factor in aquarium conditions. It was found that the weight gain of juvenile carp treated with the cryobiological supplement ‘Immunolife-Fish’ was higher than that of the control group at all stages of the study. The increase was most pronounced during the first 42 days of feeding. A significant increase in the number of leukocytes was found in the blood of fish (common carp and silver carp) exposed to a heat stressor compared to control values. In fish that received a supplement of the herbal preparation during stress, this indicator only tended to increase. The effect of the stressor in fish of both species is manifested by a significant increase in the leukocyte content, a decrease in the indicators characterizing the functional state of cellular immunity — phagocytic activity and number of phagocytes, as well as a tendency to decrease the phagocytic index of neutrophils in the blood. The use of the cryobiological supplement ‘Immunolife-Fish’ in fish exposed to stress not only prevented the decrease in the phagocytic activity of neutrophils but also contributed to a slight increase in their activity in comparison with the control. A significant decrease in the activity of lysozyme and the level of circulating immune complexes, as well as an increase in the content of seromucoids, was found in the blood serum of common and silver carp under the influence of the stress factor. The use of the drug against the background of stress leveled the negative changes in the above indicators characterizing the state of the humoral link of innate immunity. In addition, a mild immune stimulating effect of the cryobiological supplement on fish of both species, which were kept in optimal conditions, was noted. The research is aimed at the development and testing of organic, environmentally friendly anti-stress and immunostimulant agents in commercial fish farming

Further biological characterization of small molecules UM171 and SR1: In vitro effects on three hematopoietic cell populations from human cord blood

Small molecules UM171 and SR1 have already been taken into clinically-oriented protocols for the ex vivo expansion of hematopoietic stem (HSCs) and progenitor (HPCs) cells. In order to gain further insight into their biology, in the present study we have assessed their effects, both individually and in combination, on the in vitro long-term proliferation and expansion of HSCs and HPCs contained within three different cord blood-derived cell populations: MNCs (CD34+ cells = 0.8 %), LIN− cells (CD34+ cells = 41 %), and CD34+ cells (CD34+ cells >98 %). Our results show that when added to cultures in the absence of recombinant stimulatory cytokines, neither molecule had any effect. In contrast, when added in the presence of hematopoietic cytokines, UM171 and SR1 had significant stimulatory effects on cell proliferation and expansion in cultures of LIN− and CD34+ cells. No significant effects were observed in cultures of MNCs. The effects of both molecules were more pronounced in cultures with the highest proportion of CD34+ cells, and the greatest effects were observed when both molecules were added in combination. In the absence of small molecules, cell numbers reached a peak by days 25–30, and then declined; whereas in the presence of UM171 or/and SR1 cell numbers were sustained up to day 45 of culture. Our results indicate that besides CD34+ cells, LIN− cells could also be used as input cells in clinically-oriented expansion protocols, and that using both molecules simultaneously would be a better approach than using only one of them.

Gamma-aminobutyric acid elicits H2O2 signalling and promotes wheat seed germination under combined salt and heat stress.

In the realm of wheat seed germination, abiotic stresses such as salinity and high temperature have been shown to hinder the process. These stresses can lead to the production of reactive oxygen species, which, within a certain concentration range, may actually facilitate seed germination. γ-aminobutyric acid (GABA), a non-protein amino acid, serves as a crucial signaling molecule in the promotion of seed germination. Nevertheless, the potential of GABA to regulate seed germination under the simultaneous stress of heat and salinity remains unexplored in current literature. This study employed observational methods to assess seed germination rate (GR), physiological methods to measure H2O2 content, and the activities of glutamate decarboxylase (GAD), NADPH oxidase (NOX), superoxide dismutase (SOD), and catalase (CAT). The levels of ABA and GABA were quantified using high-performance liquid chromatography technology. Furthermore, quantitative real-time PCR technology was utilized to analyze the expression levels of two genes encoding antioxidant enzymes, MnSOD and CAT. The findings indicated that combined stress (30 °C + 50 mM NaCl) decreased the GR of wheat seeds to about 21%, while treatment with 2 mM GABA increased the GR to about 48%. However, the stimulatory effect of GABA was mitigated by the presence of ABA, dimethylthiourea, and NOX inhibitor, but was strengthened by H2O2, antioxidant enzyme inhibitor, fluridone, and gibberellin. In comparison to the control group (20 °C + 0 mM NaCl), this combined stress led to elevated levels of ABA, reduced GAD and NOX activity, and a decrease in H2O2 and GABA content. Further investigation revealed that this combined stress significantly suppressed the activity of superoxide dismutase (SOD) and catalase (CAT), as well as downregulated the gene expression levels of MnSOD and CAT. However, the study demonstrates that exogenous GABA effectively reversed the inhibitory effects of combined stress on wheat seed germination. These findings suggest that GABA-induced NOX-mediated H2O2 signalling plays a crucial role in mitigating the adverse impact of combined stress on wheat seed germination. This research holds significant theoretical and practical implications for the regulation of crop seed germination by GABA under conditions of combined stress.

Rice plants treated with biochar derived from Spirulina (Arthrospira platensis) optimize resource allocation towards seed production.

The use of biofertilizers is becoming an economical and environmentally friendly alternative to promote sustainable agriculture. Biochar from microalgae/cyanobacteria can be applied to enhance the productivity of food crops through soil improvement, slow nutrient absorption and release, increased water uptake, and long-term mitigation of greenhouse gas sequestration. Therefore, the aim of this study was to evaluate the stimulatory effects of biochar produced from Spirulina (Arthrospira platensis) biomass on the development and seed production of rice plants. Biochar was produced by slow pyrolysis at 300°C, and characterization was performed through microscopy, chemical, and structural composition analyses. Molecular and physiological analyses were performed in rice plants submitted to different biochar concentrations (0.02, 0.1, and 0.5 mg mL-1) to assess growth and productivity parameters. Morphological and physicochemical characterization revealed a heterogeneous morphology and the presence of several minerals (Na, K, P, Mg, Ca, S, Fe, and Si) in the biochar composition. Chemical modification of compounds post-pyrolysis and a highly porous structure with micropores were observed. Rice plants submitted to 0.5 mg mL-1 of biochar presented a decrease in root length, followed by an increase in root dry weight. The same concentration influenced seed production, with an increase of 44% in the number of seeds per plant, 17% in the percentage of full seeds per plant, 12% in the weight of 1,000 full seeds, 53% in the seed weight per plant, and 12% in grain area. Differential proteomic analyses in shoots and roots of rice plants submitted to 0.5 mg mL-1 of biochar for 20 days revealed a fine-tuning of resource allocation towards seed production. These results suggest that biochar derived from Arthrospira platensis biomass can stimulate rice seed production.

Osteoarthritis in athletes – surgical and non-surgical aspects of treatment

Introduction: Osteoarthritis (OA) is a degenerative chronic process that primarily affects the elderly, but there are also reports of OA in the young and sports-active population. Symptoms of OA usually include pain, joint stiffness, and crepitation. Physical, radiologic, and laboratory tests are required for diagnosis. The main goal of OA treatment is to restore normal function to the joint affected by OA. Methods: A literature search on PubMed was used to collect facts about the relationship between sports and OA and possible treatment methods. All forms of articles were considered in the literature search. Articles that were not written in English were not included. Literature from the library of the Faculty of Medicine in Mostar was an additional source of information. Results: OA occurs more frequently in people who play or have played professional sports. On the other hand, daily exercise has a positive effect on cartilage metabolism. Most articles reported the occurrence of OA in former professional athletes. The joint most commonly affected by OA was the knee. Injuries were a predictive factor for the occurrence of OA, and most studies talk about anterior cruciate ligament injuries. Running and football were the most commonly studied sports. Treatment options include conservative treatment, surgical methods, and biological therapy. Conclusion: Frequent strain and injury in athletes can cause cartilage damage and potentially lead to the development of OA. The benefits of exercise far outweigh the risks of developing OA. In addition, some studies report a significant stimulating effect of exercise on cartilage cell metabolism. Treatment of OA consists of the use of analgesics, intra-articular therapy, and physiotherapy. Surgical methods are considered when conservative approaches are unsuccessful. Biological therapy and similar treatments are the new potential treatments.

Development of a zoledronate-modified functional alginate hydrogel with effects of promoting osteogenesis for potential osteoporosis treatment

Osteoporosis is one of the most prevalent age-related diseases worldwide. It is characterized by a systemic deterioration in bone strength (bone density and bone mass), leading to an increase in fragility fractures. The complex pathological environment of osteoporosis presents a significant challenge to the induction of bone regeneration under osteoporotic conditions. Therefore, the development of a system for local delivery of active substances with osteoinductive effects is of practical significance in the clinical treatment of osteoporosis. In this study, we successfully loaded the anti-osteoporotic small molecule drug zoledronate (ZOL) into calcium alginate to prepare a biologically functional hydrogel, designated as ALG-ZOL-Ca. The prepared ALG-ZOL-Ca hydrogel gels quickly, making the hydrogel easy to inject and adapt to irregularly shaped bone defects, and simultaneously exhibits good bioactivity and osteoconductivity. The RT-qPCR results suggested that this hydrogel effectively promoted the expression levels of β-catenin and Axin2, which indicating a stimulative effect on the Wnt/β-catenin pathway in vitro. Moreover, ALG-ZOL-Ca hydrogel effectively promoted the expression of the OCN and SP7 genes. Therefore, this study proposes a new functional composite hydrogel that provides a potential treatment strategy for osteoporosis.

Chitosan and its derivatives regulate lactic acid synthesis during milk fermentation.

The influence of chitosan's physicochemical characteristics on the functionality of lactic acid bacteria and the production of lactic acid remains very obscure and contradictory to date. While some studies have shown a stimulatory effect of oligochitosans on the growth of Lactobacillus spp, other studies declare a bactericidal effect of chitosan. The lack and contradiction of knowledge prompted us to study the effect of chitosan on the growth and productivity of L. bulgaricus in the presence of chitosan and its derivatives. We used high molecular weight chitosan (350 kDa) and oligochitosans (25.4 and 45.3 kDa). The experiment was carried out with commercial strain of L. bulgaricus and the low fat skim cow milk powder reconstituted with sterile distilled water. After fermentation, dynamic viscosity, titratable acidity, pH, content of lactic acid, colony forming units, chitosan and oligochitosans radii were measured in the samples. Fermented dairy products were also examined using sodium dodecyl sulfate electrophoretic analysis, gas chromatography-mass spectrometry and light microscopy. The results of the study showed that when L. bulgaricus was cultured in the presence of 25.4 kDa oligochitosans at concentrations of 0.0025%, 0.005%, 0.0075% and 0.01%, the average rate of LA synthesis over 24 hours was 11.0 × 10-3 mol/L/h, 8.7 × 10-3 mol/L/h, 6.8 × 10-3 mol/L/h, 5.8 × 10-3 mol/L/h, respectively. The 45.3 kDa oligochitosans had a similar effect, while the average rate of lactic acid synthesis in the control sample was only 3.5 × 10-3 mol/L/h. Notably, 350 kDa chitosan did not affect the rate of lactic acid synthesis compared with the control sample. Interestingly, interaction of chitosan with L. bulgaricus led to a slowdown in the synthesis of propanol, an increase in the content of unsaturated and saturated fatty acids, and a change in the composition and content of other secondary metabolites. The quantity of L. bulgaricus in a sample with 0.01% chitosan exceeded their content in the control sample by more than 1,700 times. At the same chitosan concentration, the fermentation process was slowed down, increasing the shelf life of the fermented milk product from 5 to 17 days while maintaining a high content of L. bulgaricus (6.34 × 106 CFU/g).