Abstract Sexual and reproductive health (SRH) is a key dimension of overall health and wellbeing of individuals. Comprehensive Sexuality Education (CSE) is one of the most important means of promoting and supporting youth is their sexual and overall development, and a key component of HIV and sexually transmitted infections (STIs) prevention. It is widely recognised that school plays a central role in learning about health and health promotion skills, including sexuality and sexual health. Nevertheless, there is often a lack of evaluation of CSE implementation in school curriculums. This is why UNFPA (United Nations Population Fund) and BZgA (Bundeszentrale für gesundheitliche Aufklärung), in cooperation with UNESCO((United Nations Educational, Scientific and Cultural Organization), WHO (World Health Organization) and IPPF EN (International Planned Parenthood Federation), conducted a study in Moldova, Georgia, Tajikistan and Kyrgyzstan to assess the status of CSE. In this workshop, we will describe the study process, reflect on the main findings in four countries and discuss two case studies of Moldova and Georgia. The speakers will also share their experiences of translating these findings into advocacy measures and achievements since the study conducted in 2022. The data was collected using the standardized Sexuality Education Review and Assessment Tool (SERAT) via desk reviews of relevant policies, curricula and national data, questionnaires, stakeholder interviews (e.g., with teachers, representatives of technical working groups and ministries of education, young people) and discussions to validate the findings. Overall, we have seen that all four countries teach sexuality education, which is supported by adequate legal and policy frameworks, and integrate it into their school curricula. The comprehensiveness of the curriculum, teacher training and learning methods differ across the four countries. In Georgia, the biggest CSE-related challenge is that there is a stigma in society around sexuality-related issues and there is a need to support an open dialog with key stakeholders. In Moldova, this was the second SERAT assessment within 5 years, which demonstrated a significant progress in the development of sexuality education program since 2017, achieving a much higher score of compliance with the international standards for CSE. Key messages • This study shows that the four countries assessed are on a positive track in implementing and expanding their sexuality education programmes, which are supported by a legal and policy environment. • Gaps still remain in teacher training, monitoring and evaluation, and the comprehensiveness of the delivered content.