This study investigates the influence of moisture and Thermo-hygroscopic influence on bio-inspired helicoidally laminated composite plates (BHLC) for strengthening damaged reinforced concrete (RC) beams. Borrowing inspiration from nature’s design principles, BHLC plates represent a novel approach to rehabilitation. An analytical approach based on deformation compatibility is employed, assuming constant shear and normal stresses across the adhesive layer within the framework of linear elasticity. BHLC plates are implemented to restrict crack propagation within the RC beam. The analytical methodology considers equilibrium and deformation compatibility across all components: the concrete beam, BHLC plate, and adhesive layer. A parametric study explores the sensitivity of interfacial stresses to parameters such as laminate and adhesive stiffness, plate thickness, and helicoidal layup configuration. The results reveal a significant influence of these factors on the magnitude of maximum interfacial shear and normal stresses. This study establishes a foundation for future analyses of damaged RC beams strengthened with BHLC plates, potentially leading to advancements in rehabilitation methodologies and improved crack propagation modeling in RC beams.