The research work was conducted at Jute Agriculture Experimental Station (JAES) Manikganj and Jute Agriculture Regional Station (JARS) Faridpur of Bangladesh. The present study aimed at determining the nutritional requirement for optimum growth and yield of an advanced olitorius breeding line of O-0512-6-2 in Bangladesh. The experiment was laid out in RCBD with 10 treatments having three replications during the year 2019, 2020 and 2021. The results indicated significant effect on yield and yield contributing characters over control with different fertilizer (NPKS) levels of advanced olitorius breeding line of O-0512-6-2. The highest number of plant height, base diameter, green weight with leaves and without leaves was observed by the treatment T3 (N100P10K25S20) which is statistically significant. The highest fiber yield (2.75 t ha−1) and stick yield (6.50 t ha−1) at Manikganj and highest fiber yield (2.58 t ha−1) and stick yield (6.09 t ha–1) at Faridpur was also obtained by the combined dose of N100P10K25S20 (T3) kg ha−1. Therefore, this combination N100P10K25S20 Kg ha−1 seemed to be optimum for maximum growth and yield of advanced olitorius breeding line of O-0512-6-2. Bangladesh Agron. J. 2024, 26(2): 1-6