Sterile Aquades Research Articles

Potensi Ekstrak Buah Delima Merah (Punica granatum Linn) terhadap Penurunan Jumlah Koloni Streptococcus mutans

Streptococcus mutans is a bacteria that initiates the formation of plaque on the tooth surface. S. mutans works by fermenting carbohydrates to produce an acidic atmosphere, so the plaque pH becomes low, this condition can lead to demineralization of enamel and dentin commonly called caries. One of alternative treatment to reduce the population of S. mutans by using herbal plants, namely red pomegranate. Red pomegranate contains flavonoids, tannins, and alkaloids as antibacterial. This study aimed to determine the inhibition of red pomegranate extract on growth of S. mutans. The method used is by counting the number of S. mutans using a colony counter. This method with 4 samples in each study group. The study group consists of 4 treatment groups (25%, 50%, 75% and 100% red pomegranate extract), positive control group (chlorhexidine), and negative control group (sterile aquades). Data were analyzed using Kruskal-Wallis Test and Mann-Whitney test. Red pomegranate extract has the ability to inhibit the growth of S. mutans. The concentration of red pomegranate extract that has the greatest inhibitory effect on S. mutans growth is 100%.
 Keyword: Antibacterial, Streptococcus mutans, caries, red pomegranate fruit extract

Daya Hambat Ekstrak Buah Delima Merah (Punica granatum Linn) terhadap Pertumbuhan Staphylococcus aureus (The Inhibition of Red Pomegranate Fruit Extract (Punica granatum Linn) on The Growth of Staphylococcus aureus)


 Background: Staphylococcus aureus is the most common microorganism in angular cheilitis. Angular cheilitis is inflammation in one corner of the mouth or both corners of the mouth may extend involving the lips commisura and surrounding skin. The prevalence of angular cheilitis is 0.2-15.1% in children and 0.7-3.8% in adults. One of alternative treatment by using herbal plants, namely red pomegranate. Red pomegranate contains flavonoids, tannins, and alkaloids as antibacterial. Objective: to determine the inhibition of red pomegranate extract on growth of Staphylococcus aureus. Methods: disk diffusion method with 6 samples in each study group. The study group consisted of 4 treatment groups (25%, 50%, 75% and 100% red pomegranate extract), positive control group (fusidic acid), and negative control group (sterile aquades). Data were analyzed using One Way Anova test and LSD (Least Significant Difference) test. Results and conclusions: red pomegranate extract has the ability to inhibit the growth of Staphylococcus aureus. The concentration of red pomegranate extract has the greatest inhibitory effect on S. aureus growth, that is 75% and 100%.
 Keyword: Antibacterial activity, red pomegranate fruit extract, Staphylococcus aureus, disk diffusion method

PERBANDINGAN KEBOCORAN MIKRO PADA RESTORASI KOMPOSIT DENGAN PEMAKAIAN BONDING GENERASI KELIMA DAN BONDING GENERASI KETUJUH

Composite resin is the most frequently material used for restoring teeth but still failure cases are seen which leading to microleakage. Microleakage might be attributed to various factors, one of them is bonding agent. Various generations of bonding agents have been introduced to overcome the microleakage. The aim of this study was to evaluate the microleakage of composite restorations using the fifth and seventh bonding agent. Class I cavities (3X2X2 mm) were prepared on the occlusal surfaces of 32 human upper premolars. Teeth were classified into two groups according to the type of bonding agent used (n =16). Group I: Fifth Generation of Bonding Agent-Adper Single Bond2. Group II: Seventh Generation of Bonding Agent-Single Bond Universal. All cavities were restored with Filtek Z250 XT composite resin, stored in sterile aquades water at 370C for 24 h. The root apices were sealed with sticky wax, and all the surfaces, except for 2 mm from the margins, were coated with nail varnish. The teeth were immersed in a 1% methylene blue dye solution for 24 h, and then rinsed in running water, blot-dried and sectioned longitudinally through the center of restorations from the buccal to palatal surface. The sections were blindly assessed for microleakage of dye penetration by using a stereomicroscope. Data were collected and statistically analyzed by independent t -test. There was no significant difference (p>0,05) between two groups. The conclusion is fifth generation of bonding agent revealed less leakage compared to the seventh generation even statistically there was no significant difference.
 Keywords:Microleakage, fifth generation of bonding agent, seventh generation of bonding agent.

The effect of the thermoplastic nilon enterprises (valplast) on clove extract (syzygium aromaticum) in various concentrations on the color change

Objective: To investigate the effect of soaking of thermoplastic nylon (valplast) on clove flower extract (syzygium aromaticum) as a denture cleanser with different concentration 0.8%, 1%, 1.2%, 1.4%, and 1.6% to the color change of thermoplastic nylon.Material and Methods: 30 samples were divided into 6 groups. The sample size is (10x10x1) mm. The first group was immersed in sterile aquades and the other groups were immersed in clove flower extracts of 0.8%, 1%, 1.2%, 1.4%, and 1.6% for 12 days. The light intensity then measured using densitometer.Results: One way anova test results obtained value 0.174 (p>0.05) which indicates that there is no significant difference in each group.Conclusions: This study are darker discoloration of the thermoplastic nylon at a concentration of 1.6% and lower color changes occurring at a concentration of 0.8%.

The inhibition of Streptococcus mutans glucosyltransferase enzyme activity by mangosteen pericarp extract

Background: Streptococcus mutans (S. mutans) is a bacterium that plays an important role in the pathogenesis of dental caries. Streptococcus mutans produces the glucosyltransferase enzyme which is capable of catalyzing glucan synthesis in the progression of dental caries. Certain treatments involving traditional plant use have been developed to eradicate Streptococcus mutans as a means of preventing the formation of dental caries. One of these is mangosteen pericarp extract containing a number of polyphenols that have the capacity to act as antibacterial agents, namely; tannin, mangostin, and flavonoid. Purpose: The research aimed to investigate the inhibitory power of mangosteen pericarp extract against Streptococcus mutans producing the glucosyltransferase enzyme. Methods: The research used mangosteen pericarp extract at concentrations of 0.39% and 0.78% as the treatments, while 0.12% chlorhexidine gluconate was used as a positive control, and distilled water as a negative control. Each group consisted of six samples. Mangosteen peels extracted with 96% ethanol (maceration method) and mangosteen extract constituted 5% of the total weight of the mangosteen pericarp. Supernatant containing Gtf enzyme produced from a culture medium and centrifuged at 1500 rpm for 10 minutes at 4o C. Glucosyltransferase enzyme activity was measured by analyzing the extensive fructose area by means of High Performance Liquid Chromatography (HPLC). The extensive fructose area was determined according to time retention in each group. Results: Mangosteen peel extract at concentrations of 0.39% and 0.78% demonstrated greater ability than the negative control group (sterile aquades) and similar ability to the positive group (chlorhexidine 0.12%) to inhibit the activity of the Gtf enzyme or S. mutans bacteria. Conclusion: Mangosteen pericarp extract has the ability to inhibit the activity of Streptococcus mutans in producing glucosyltransferase enzyme.

The Morphological Changes of Staphylococcus Aureus Caused by Ethanol extracts of Cocoa Beans (Theobama Cacao) through In Vitro

Staphylococcus aureus (S. aureus) is positive gram bacteria which often infect humans. The use of antibiotic for therapy is the factor of resistance. S. aureus resistant towards some biotic like tetracycline, streptomycin, chloramphenicol, ampicilin, eritromisin and penicillin. The increase of the resistance demands more findings on new drugs. Ethanol extracts of cocoa beans has a block capacity towards the growth of S. aureus via in vitro. The effect of antibacterial of ethanol extracts has already been proved. However, its morphology changes on S. aureus after given ethanol extracts of cocoa beans is not found yet. This research is aimed at studying the morphological changes on S. aureus after given ethanol extracts of cocoa beans. The research design is Quasi Experimental with Posttest Only Control Group Design. The sample used is S. aureus whereas the material tests is ethanol extracts of cocoa beans in concentration of 31.2 mg/mL; 15.6 mg/mL and 7.8 mg/mL. The negative control is sterile aquades and the positive control is cephalexin suspension 4 ug/mL. The method for observing the S. aureus morphology change is scanning electronic microscope. The research result shows that there is a morphology change. Given ethanol extracts of cacao seed at 7,8 mg/mL and 15,6 mg/mL, there is enlargement of wall cell and the unity of septum. And at 31,25 mg/mL, there is protrusion at the wall cell and enlargement of wall cell.Keywords: Antimikrobial, Cacao, SEM, Staphylococcus aureus.

KEKASARAN PERMUKAAN RESIN KOMPOSIT NANOFILLER SETELAH PERENDAMAN ALAM AIR SUNGAI DAN AIR PDAM

ABSTRACT Background :One of composite resin kinds is often used for tooth restoration, it is nanofilled composite resin. Nanofilled composite resin is a restoration material containing nano size filler particle, so that it can repair the physical characteristic of composite such as reducing the surface roughness. People in Barito Kuala especially in Desa Anjir Pasar, besides using tap water, they also still use water from river for daily use. The water of river in Desa Anjir Pasar has acid characteristic with Ph range 3-5. An acidic environment causes surface roughness of nanofilled composite resin which allow the secunder caries.Purpose : The aim of this research was to know the difference in surface roughness of nanofilled composite resin after being immersed in river water, tap water, and sterile aquades. Method :This research was a true experimental research with post test only and control group design, used nanofilled composite resin disc samples with diameter of 10 mm and 2 mm thick divided into 3 groups of treatment. Each group was immersed in sterile aquades (as control group), tap water, and river water with acid Ph for 8 days (equals to 3 years of exposure), then the surface roughness was measured using surface roughness measurement. Result : Data was tested using parametric analysis one way anova 95% ( = 0,05) and it obtained p = 0,000 (p = < 0,05). Based on the result it can be concluded that there was significant difference in surface roughness on nanofilled composite resin which had been immersed in each water sample for 8 days. Conclusion : Nanofilled resin composite immersed in river water had higher mean value of surface roughness compared to immersion in sterile aquades and tap water. Keywords: Resin Nanofilled Composite, River Water, Tap Water, Surface Roughness

KEBOCORAN TEPI RESTORASI RESIN KOMPOSIT NANOHYBRID SETELAH PERENDAMAN DALAM AIR SUNGAI DESA ANJIR PASAR

ABSTRACT Background: Composite resin is broadly used by dental practitioners as restoration material to treat caries. Nanohybrid composite resin is a type of composite resin composed of nano-size filler combined with larger filler. Composite resin has water absorption characteristic which tends to solute when it reacts with acid (low pH). Result from both of those characteristics is formation of a gap between restoration and cavity edge, which eventually leads to micro-leakage. Purpose: The aim of this study was to assess and measure the rate of nanohybrid composite resin micro-leakage as a result of submersion in water with acid characteristic (low pH). Methode: This study was a true experimental using posttest-only with control group design. Samples used were 20 maxillary premolars, divided into 2 groups: river water immersion which has acid characteristic (low pH) as treatment group, and sterile aquades immersion which has neutral characteristic (pH 7) as control group. Result: The result of Mann-Whitney showed a significant difference between the treatment group with the mean score of 4,00 and control group with the mean score of 3,00. Conclusion: Based on the study it can be concluded that there was a significant difference in micro-leakage effect of river water immersion and sterile aquades immersion on nanohybrid resin composite restorasion. Keywords: nanohybrid composite resin, acid pH, micro-leakage.