Stereum Sp Research Articles

Diversity of Macrosscopic Fungi in the Cibereum Waterfall Path, Mount Gede Pangrango National Park (TNGGP) West Java

Indonesia is a tropical country that has natural resources that are rich in biodiversity. One of these biodiversity is mushrooms. Mushrooms generally occupy various types of habitats, namely soil, wood, litter, animal waste and some grow on rotting mushrooms.. This study aims to study the diversity of Microscopic Fungi in the Cibereum Curug Route,Selabintana Resort, Gunung Gede Pangrango National Park, West Java. The research method used in this study is VES (Visual Encounter Survey) by combining transects following the Cibereum waterfall path, and taking the right and left areas of 1 meter. Data analysis was performed by qualitative descriptive analysis and quantitative descriptive analysis. The results of the research on the Curug Cibereum Resort Selabintana route, Gunung Gede Pangrango National Park (TNGGP) West Java, found as many as 30 species of basidiomycota mushrooms and 3 species from the ascomycota division. The most common species found in the study area were Coprinellus disseminatus (336 Number of individuals/4000 m2), Stereum sp (153 Number of individual/ 4000 m2), Mycena roseignicola (145 individual pieces/4000 m2), and Favolaschia pustulosa (138 Number of individual / 4000 m2). The diversity index of basidiomycota fungi in the Curug Cibereum route is in the medium category, namely H'= 1.004.

A New Sesquiterpene from Stereum sp. YMF1.04734

The chemical constituent of the fungus Stereum sp. YMF1.04734 was studied. A new sesquiterpene sterostrein V (1) and a known sesquiterpene sterostrein H (2) were isolated from the EtOAc extract of the fungal culture broth. The structure of the compound was elucidated mainly by HR-ESI-MS experiment and 1D and 2D NMR spectroscopy analysis.

Sterepinic Acids A⁻C, New Carboxylic Acids Produced by a Marine Alga-Derived Fungus.

Sterepinic acids A–C (1–3), new carboxylic acids with two primary alcohols, have been isolated from a fungal strain of Stereum sp. OUPS-124D-1 attached to the marine alga Undaria pinnatifida. Dihydro-1,5-secovibralactone (4), a new vibralactone derivative, was isolated from the same fungal metabolites together with known vibralactone A (5), and 1,5-secovibralactone (6). The planar structures of these compounds have been elucidated by spectroscopic analyses using IR, HRFABMS, and NMR spectra. To determine the absolute configuration of the compounds, we used the phenylglycine methyl ester (PGME) method. These compounds exhibited less activity in the cytotoxicity assay against cancer cell lines.

A New Sesquiterpenoid from the Fungus Stereum sp. YMF1.1686

The chemical constituent of the fungus Stereum sp. YMF1.1686 has been studied. One new sesquiterpenoid sterostrein U (1) was isolated from the EtOAc extract of the culture broth. The structure of the compound was elucidated mainly by 1D and 2D NMR spectroscopy and HR-ESI-MS experiments.

Miscellaneous lanostane triterpenoids with cytotoxicities from fruiting bodies of the basidiomycete Stereum sp.

Ten new highly oxygenated lanostane triterpenoids, stereinones A–J (1–10), were isolated from the fruiting bodies of the basidiomycete Stereum sp. Compounds 3 and 4 are structurally characterized as intact lanostane-type triterpenoids containing unusual 1,2-diketone functionality at C-11 and C-12, while compound 10 is a 24-methylene-lanostane. The structures of these new compounds were established based on detailed 1D and 2D NMR spectroscopic analyses, along with quantum chemical NMR calculations. All isolates were evaluated for their in vitro cytotoxicities against five human tumor cell lines (including HL-60, A-549, SMMC-7721, MCF-7, and SW480 cell lines). Compound 4 showed moderate cytotoxic activities against tumor cell lines SMMC-7721 and SW480 with IC50 values of 9.1 and 9.8μM, respectively.

Lanostane triterpenoids from fruiting bodies of basidiomycete Stereum sp., structures and biological activities.

Twelve new lanostane triterpenoids, sterenoids A-L (1-12) have been isolated from fruiting bodies of the basidiomycete Stereum sp. Compounds 1-12 are rare 14(13→︀12)abeo-lanostane triterpenoids featuring remarkable 13R configurations that discriminate from the previously covered counterparts. Their structures and absolute configurations are assigned on the basis of in-depth one- and two-dimensional NMR spectroscopic analysis, as well as unbiased quantum chemical NMR and electronic CD calculations. All isolates are evaluated for their in vitro cytotoxicity against five human tumor cell lines. Compound 5 exhibits potent cytotoxic activities against tumor cell lines HL-60 and SMMC-7721 with IC50 values of 4.7 and 7.6 μM, respectively.

Chemical constituents from the fungus Stereum sp. YMF1.04183

The chemical constituents of the basidiomycete Stereum sp. YMF1.04183 were studied. Three new sesquiterpenes, stereumenes A–C (1–3), were isolated and identified from the EtOAc extract of Stereum sp. YMF1.04183 culture broth. Stereumene B showed weak nematicidal activity against Caenorhabditis elegans.

Three new sesquiterpenes from the fungus Stereum sp. YMF1.1686

A basidiomycete fungus, Stereum sp. YMF1.1686, was studied for its ability to produce new sesquiterpenes on nutrient-enriched cultivation media. Three new sesquiterpenes sterostrein R (1), sterostrein S (2) and sterostrein T (3), along with two known compounds sterostrein A (4) and sterostrein C (5) were isolated from the EtOAc extract of a culture broth of Stereum sp. YMF1.1686.

Two new sesquiterpenes from the fungus Stereum sp. NN048997

Two new sesquiterpenes, sterostreins P (1) and Q (2) along with a known compound sterostrein H (3) were isolated from the EtOAc extract of a culture broth of Stereum sp. NN048997. Their structures were elucidated based on spectroscopic data from 1D, 2D NMR and HRMS experiments. Compound 1 exhibited weak nematicidal activity against the Caenorhabditis elegans nematode.

Stereumins K–P, sesquiterpenes from the fungus Stereum sp. CCTCC AF 2012007

The basidiomycete genus Stereum fam Stereaceae is a rich source of sesquiterpenoids. This phytochemical investigation of the basidiomycete Stereum sp. CCTCC AF 2012007 led to isolation of six cadinane-type sesquiterpenes named stereumins K-P. Stereumin O was very likely an artifact formed from stereumin N during isolation. Their structures were elucidated mainly by 1D and 2D NMR spectroscopy. Structures of stereumins K, L, M and P were further confirmed by single-crystal X-ray diffraction, and the absolute configuration of stereumin K was determined.

Coexisting Curtobacterium Bacterium Promotes Growth of White-Rot Fungus Stereum sp.

White-rot basidiomycetes are the main decomposers of woody biomass in forest ecosystems. Little is known, however, about the interactions between white-rot fungi and other microorganisms in decayed wood. A wood-rotting fungus, Stereum sp. strain TN4F, was isolated from a fruit body, and its coexisting cultivable bacteria were isolated from its substrate; natural white-rot decayed wood. The effects of bacteria on fungal growth were examined by confrontational assay in vitro. A growth-promoting bacterium for this Stereum strain was identified as Curtobacterium sp. TN4W-19, using 16SrRNA sequencing. A confrontational assay revealed that Curtobacterium sp. TN4W-19 significantly promoted the mycelial growth of Stereum sp. TN4F in the direction of the bacterial colony, without direct contact between the mycelium and bacterial cells. This is the first report of a positive interaction between a white-rot fungus and a coexisting bacterial strain in vitro.

Two new benzofuran derivatives from the fungus Stereum sp. YMF1.1684

A basidiomycete fungus, Stereum sp. YMF1.1684, was studied for its ability to produce new compounds on nutrient-enriched cultivation media. One new benzofuran, 2-hydroxy-1-(( S)-2-(prop-1-en-2-yl)-2,3-dihydrobenzofuran-5-yl)propan-1-one, named as phenostereum A ( 1) and one novel dimer benzofuran, named as phenostereum B ( 2) were isolated from EtOAc extract of the culture broth of the fungus Stereum sp. YMF1.1684. The structures were elucidated using spectroscopic data from 1D, 2D NMR and HRESIMS experiments.

Stereumins H−J, Stereumane-Type Sesquiterpenes from the Fungus Stereum sp.

Stereumins H (1), I (2), and J (3), three sesquiterpenes possessing a novel stereumane-type backbone, were isolated from an extract of culture broth of the Basidiomycete Stereum sp. CCTCC AF 207024. The complete structural assignments including the absolute configurations are reported by using X-ray studies and density functional theory at the B3LYP/aug-cc-pVDZ//B3LYP/6-31G(d) level.

Two New Sesquiterpenes from the Fungus Stereum sp.

AbstractTwo new sesquiterpenes, stereumin F (1) and stereumin G (2), together with two known compounds (3β,5α,22E,24R)‐5,8‐epidioxyergosta‐6,22‐dien‐3‐ol (3) and 4‐(2‐hydroxyethyl)phenol (4), were isolated from the AcOEt extract of the culture broth and the MeOH extract of the mycelium of the fungal strain Stereum sp. CCTCC AF 207024. Their structures were established on the basis of spectral analyses.

Sterelactones: New Isolactarane Type Sesquiterpenoids with Antifungal Activity from Stereum sp. IBWF 01060

Four members of a new family of tetracyclic sesquiterenoids possessing the isolactarane skeleton have been isolated from mycelial cultures of Stereum sp. IBWF 01060. Their structure elucidation and their antifungal activity against several plant pathogens as well as other microorganisms are reported.

Stereumin A–E, sesquiterpenoids from the fungus Stereum sp. CCTCC AF 207024

Five cadinane sesquiterpenoids, named stereumin A ( 1), B ( 2), C ( 3), D ( 4) and E ( 5) were isolated from the CHCl 3 extract of the culture broth of the fungal strain CCTCC AF 207024. Based on the sequences at the internal transcribed spacer (ITS) region and partial 28S rDNA, this fungus was identified as a Stereum sp. The structures of the five compounds were elucidated using spectroscopic data from 1D, 2D NMR and HRESIMS experiments, and the structures of 1 and 2 were further confirmed by single-crystal X-ray diffraction analysis. Compounds 1– 5 showed nematicidal activities against the nematode Panagrellus redivivus at 400 mg l −1. Among these five compounds, compounds 3 and 4 killed 84.4% and 94.9% of P. redivivus, respectively in 48 h.

Sterenin A, B, C and D, Novel 11β-Hydroxysteroid Dehydrogenase Type 1 Inhibitors from Stereum sp. SANK 21205

The novel 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) inhibitors known as sterenin A, B, C and D were found in a solid-state culture of the producing basidiomycetes identified as Stereum sp. SANK 21205. Purification of the 50% aq Me(2)CO extract of the culture was performed by EtOAc extraction, reversed phase open-column chromatography and successive ODS HPLC preparation. These compounds, whose structures were determined by several spectroscopic methods, were found to be novel isoindolinone alkaloids which exhibited potent selective inhibitory activities against 11beta-HSD1.

The Chemical Constituents of the Fungus Stereum sp.

Four new compounds, including a sesquiterpene and three aromatic compounds, and a known compound were isolated from a culture broth of the fungus Stereum sp. The novel sesquiterpene was determined to be stereumone A ((+)-2,3,4a,5,6,7,8a,9-octahydro-5-hydroxy-6,6,9-trimethyl-4,8a-epoxynaphtho[2,3-b]furan-8(8H)-one; 1), and the three new aromatic compounds were elucidated as 3,5-dihydroxy-4-(3-methylbut-2-enyl)benzene-1,2-dicarbaldehyde (2), 5,7-dihydroxy-6-(3-methylbut-2-enyl)isobenzofuran-1(3H)-one (3), butyl 2,4-dihydroxy-6-methylbenzoate (4), together with the known compound methyl 2,4-dihydroxy-6-methylbenzoate (5). The structures were established by spectroscopic methods including 2D-NMR techniques. Compounds 2 and 4 showed evident nematicidal activity against nematode Panagrellus redivivus.

Degradation of sugar cane bagasse by several white‐rot fungi

AbstractForty‐two white‐rot fungi isolated in South America were incubated with long fibre sugar cane bagasse (LFB). The residual composition of LFB was determined after white‐rot decay at 30 and 60 days. The ratio of residual lignin to residual lignin to residual cellulose (RL/RC) of untreated material (LFB) was 0.48. After white‐rot‐decay, the residual material with lower RL/RC ratios indicated that mainly lignin was degraded. In only 30 days, Phlebia sp. MVHC 5535, Athelia sp. MVHC 5509 and Spongipellis pachyodon MVHC 5019 caused a decrease in the RL/RC ratio to 0.36, 0.37 and 0.38, respectively, while it took 60 days for Ganoderma applanatum MVHC 5347, Hyphodontia sp. MVHC 5544, Panus tigrinus MVHC 5400, Stereum sp. MVHC 5113, Phellinus punctatus MVHC 5346 and MVHC 6388 to reach a ratio lower than 0.40. No correlation was found between the amount of some ligninolytic enzymes secreted and the residual composition of bagasse after white‐rot fungi fermentation. Most of the fungal strains caused an increase in the relative amount of residual cellulose, indicating that hemicellulose was the preferred energy source.