Background: Sedentary lifestyle is among the leading cause of cardiovascular and respiratory diseases over the globe. Aerobic conditioning is among the beneficial intervention to improve pulmonary function and manage the complications caused by sedentary life style. Objective: To determine the effects of step aerobics training on pulmonary function among female students with Sedentary Lifestyle. Methods: This RCT was conducted in Margalla Institute of Health Sciences from February to July 2019. Nonprobability convenient sampling was employed to collect sample of 80 students, randomly allocated into groups (40 per group) by coin toss method. Group A performed step aerobics and Group B performed cycling for 6 weeks. Pulmonary function test (FVC, FEV1, PEF) and 3-minute step test was performed at baseline, after 2, 4 and 6 weeks in both groups. Heart rate and oxygen saturation was monitored pre and post exercise. Results: The mean age of Group A is 22.43±2.49 and Group B is 21.80±2.04. Results showed statistically significant difference in respiratory function between groups. Significant improvement of respiratory function was observed in group A which performed aerobics program as compared to group B. The level of significance was set on p <0.05. Conclusion: Step aerobics is considered beneficial in improvement of pulmonary functions and is a good alternative for conditioning program for female adults. Cycling also improved pulmonary function but the progress was slow as compared to step aerobics which showed quick impact in improving pulmonary function test. Clinical Trial Number: NCT04051788