Stem Position Research Articles

Comparative Proteomics of Mulberry Leaves at Different Developmental Stages Identify Novel Proteins Function Related to Photosynthesis.

Mulberry leaves at different positions are different in photosynthetic rate, nutrient substance and feeding impact to silkworms. Here, we investigated the proteomic differences of the first (L1), sixth (L6), and twentieth (L20) mulberry leaves at different stem positions (from top to the base) using a label-free quantitative proteomics approach. L1 contained less developed photosynthetic apparatus but was more active in protein synthesis. L20 has more channel proteins and oxidoreductases relative to L6. Proteins that detected in all measured leaves were classified into three groups according to their expression patterns in L1, L6, and L20. The protein group that displayed the maximum amount in L6 has the highest possibility that function related to photosynthesis. Nine function unknown proteins belong to this group were further analyzed in the light responsive expression, evolutionary tree and sub-cellular localization analysis. Based on the results, five proteins were suggested to be involved in photosynthesis. Taken together, these results reveal the molecular details of different roles of mulberry leaves at different developmental stages and contribute to the identification of five proteins that might function related to photosynthesis.

Crop stem detection and tracking for precision hoeing using deep learning

Developing alternatives to the chemical weeding process usually carried out in vegetable crop farming is necessary in order to reach a more sustainable agriculture. However, a precise mechanical weeding requires specific sensors and advanced computer vision algorithms to process crop and weed discrimination in real-time.In this paper we propose an algorithm able to detect, locate, and track the stem position of crops in images which is suitable for precision actions in vegetable fields such as mechanical hoeing within crop rows. The algorithm is twofold: (i) a deep neural network for object detection is first used to detect crop stems in individual RGB images and then (ii) an aggregation algorithm further refines the detections taking advantage of the temporal redundancy in consecutive frames.We evaluated the pipeline on images of maize and bean crops at an early stage of development, acquired in field conditions with a camera embedded in an experimental mechanical weeding system. We reported F1-scores of respectively 94.74% and 93.82% with a location accuracy around 0.7 cm when compared with human annotation. Moreover, this pipeline can operate in real-time on an embedded computer consuming as little power as 30 W.

Automatic segmentation of stem and leaf components and individual maize plants in field terrestrial LiDAR data using convolutional neural networks

High-throughput maize phenotyping at both organ and plant levels plays a key role in molecular breeding for increasing crop yields. Although the rapid development of light detection and ranging (LiDAR) provides a new way to characterize three-dimensional (3D) plant structure, there is a need to develop robust algorithms for extracting 3D phenotypic traits from LiDAR data to assist in gene identification and selection. Accurate 3D phenotyping in field environments remains challenging, owing to difficulties in segmentation of organs and individual plants in field terrestrial LiDAR data. We describe a two-stage method that combines both convolutional neural networks (CNNs) and morphological characteristics to segment stems and leaves of individual maize plants in field environments. It initially extracts stem points using the PointCNN model and obtains stem instances by fitting 3D cylinders to the points. It then segments the field LiDAR point cloud into individual plants using local point densities and 3D morphological structures of maize plants. The method was tested using 40 samples from field observations and showed high accuracy in the segmentation of both organs (F-score =0.8207) and plants (F-score =0.9909). The effectiveness of terrestrial LiDAR for phenotyping at organ (including leaf area and stem position) and individual plant (including individual height and crown width) levels in field environments was evaluated. The accuracies of derived stem position (position error =0.0141 m), plant height (R2 >0.99), crown width (R2 >0.90), and leaf area (R2 >0.85) allow investigating plant structural and functional phenotypes in a high-throughput way. This CNN-based solution overcomes the major challenges in organ-level phenotypic trait extraction associated with the organ segmentation, and potentially contributes to studies of plant phenomics and precision agriculture.

Strain Analysis in Cementless Hip Femoral Prosthesis using the Finite Element Method - Influence of the Variability of the Angular Positioning of the Implant.

Objective The present study aims to evaluate the influence of different positioning of the hip femoral prosthesis on the stress and strain over this implant. Methods A femoral prosthesis (Taper - Víncula, Rio Claro, SP, Brazil) was submitted to a stress and strain analysis using the finite element method (FEM) according to the International Organization for Standardization (ISO) 7206-6 Implants for surgery - Partial and total hip joint prostheses - Part 6: Endurance properties testing and performance requirements of neck region of stemmed femoral components standard. The analysis proposed a branch of the physical test with a +/- 5° angle variation on the standard proposed for α and β variables. Results The isolated +/- 5° variation on the α angle, as well as the association of +/- 5° variation on the α and β angles, presented significant statistical differences compared with the control strain ( p = 0.027 and 0.021, respectively). Variation on angle β alone did not result in a significant change in the strain of the prosthesis ( p = 0.128). The stem positioning with greatest implant strain was α = 5° and β = 14° ( p = 0.032). Conclusion A variation on the positioning of the prosthetic femoral stem by +/- 5° in the coronal plane and/or the association of a +/- 5° angle in coronal and sagittal planes significantly influenced implant strain.

Mapping tropical forest trees across large areas with lightweight cost-effective terrestrial laser scanning

Key messageWe used lightweight terrestrial laser scanning (TLS) to detect over 3000 stems per hectare across a 12-ha permanent forest plot in French Guiana, 81% of them < 10 cm in trunk diameter. This method retrieved 85% of the trees of a classic inventory. Finally, TLS revealed that stem positions of the classic inventory had geolocation errors of up to 6 m.ContextAccurate position mapping of tropical rainforest trees is crucial for baseline studies of tropical forest ecology but is labor-intensive. Terrestrial lidar scanning (TLS) is broadly used in temperate forest inventories, but its use in rainforests is restricted to the determination of individual tree volumes within small survey areas.AimsMapping tree stems across one large (12-ha) rainforest plot, including trees less than 10 cm DBH, and evaluating the precision of traditional mapping approaches.MethodsWe used lightweight TLS, co-registered the acquisitions, and developed a new efficient algorithm to process the TLS data.ResultsWe detected 36,422 stems of which 29,665 (81%) were < 10 cm in diameter at breast height (DBH). Of the trees ≥ 10 cm DBH previously censused in the plot, 85% were identified by TLS. Automatic DBH estimation from TLS data had an RMSE of 6 cm. RMSE was improved to 3 cm by a manual verification of the shape and quality of the stem points. The initial census map had substantial bias in tree geolocation with a maximum value around 6 m.ConclusionLightweight TLS technology is a promising tool for the estimation of stem tapering and volume. Here, we show that it also facilitates the establishment of large tropical forest inventories, by improving the positioning of trees, thus increasing the accuracy of forest inventories and their cost-effectiveness.

Experimental analysis of early periprosthetic femoral fractures with uncemented straight hip stems

BackgroundThe periprosthetic femoral fracture is one of the most severe complications after total hip arthroplasty and is associated with an increased mortality. The underlying causes and the patient- and implant-specific risk factors of periprosthetic femoral fractures remain insufficiently understood. The aim of this study was to gain a more profound understanding of the underlying fracture mechanisms and to provide experimental datasets for validation of computational models. MethodsSix cadaveric femurs were implanted with straight hip stems (Zweymueller design) and loaded until fracture reproducing the clinically relevant load cases stumbling and sideways fall. Displacements and the strain distribution on the surface of the femurs and implants, as well as the fracture load and implant subsidence were measured. FindingsFor the load case stumbling the mean fracture load was 6743 N and two different mechanisms leading to fracture could be identified: high subsidence with low femoral bending and small subsidence with high femoral bending. For the load case sideways fall the mean fracture load was 1757 N and both tested femurs fractured due to a rotation of the hip stem around its own axis. The detailed datasets provided by this study can be used in future computational models. InterpretationWe demonstrated that the underlying fracture mechanisms of periprosthetic femoral fractures can be fundamentally different in the load case stumbling. The seating and exact position of the hip stem in the femur may correlate with implant subsidence and therefore lead to different types of fracture mechanisms resulting in different patient-specific fracture risks.

The walls of the femoral neck as an auxiliary tool for femoral stem positioning.

Femoral anteversion is a major contributor to functionality of the hip joint and is implicated in many joint pathologies. Accurate determination of component version intraoperatively is a technically challenging process that relies on the visual estimation of the surgeon. The following study aimed to examine whether the walls of the femoral neck can be used as appropriate landmarks to ensure appropriate femoral prosthesis version intraoperatively. We conducted a retrospective study based on 32 patients (64 hips) admitted to our centre between July and September 2020 who had undergone a CT scan of their lower limbs. Through radiological imaging analysis, the following measurements were performed bilaterally for each patient: anterior wall version, posterior wall version, and mid-neck femoral version. Anterior and posterior wall version were compared and evaluated relative to mid-neck version, which represented the true version value. Mean anterior wall anteversion was 20° (95% CI, 17.6-22.8°) and mean posterior wall anteversion was -12° (95% CI, -15 to -9.7°). The anterior walls of the femoral neck had a constant of -7 and a coefficient of 0.9 (95% CI, -9.8 to -4.2; p < 0.0001; R2 0.77).The posterior walls of the femoral neck had a constant of 20 and a coefficient of 0.7 (95% CI, 17.8-22.5; p < 0.0001; R2 0.60). Surgeons can accurately obtain femoral anteversion by subtracting 7° from the angle taken between the anterior wall and the posterior femoral condyles or by adding 20° to the angle taken between the posterior wall and the posterior femoral condyles.

Clinical and radiographic outcomes after total hip arthroplasty with the NANOS neck preserving hip stem: a 10 to 16-year follow-up study

BackgroundShort-stem Hip Arthroplasty (SHA) are increasingly implanted in recent years thanks to their potential advantage in preserving metaphyseal bone-stock. Among them, the NANOS® short-stem implant demonstrated satisfactory results to short and mid-term. The purpose of this retrospective study was to evaluate the clinical and radiographic outcome of the Nanos® short stem at a minimum follow-up of 10 years.MethodsSixty-seven patients aged 53 ± 20 years were enlisted in the study, for a total of 72 hips. Primary outcomes were survivorship of the implant and clinical outcome measured using the Hip disability and Osteoarthritis Outcome scores (HOOS) and the Short Form Survey (SF12) questionnaire. The secondary outcome was a radiological evaluation calculating the inclination and the anteversion angle of the acetabular cup for each implant and investigating osteolysis, heterotopic ossifications and stem position.ResultsWe observed a 95.5% stem survivorship. The complication rate was 7.6% and three implants underwent revision because of an aseptic loosening, an infection and a periprosthetic fracture due to trauma.Among 58 patients (63 hips) evaluated in an outpatient visit 10–16 years after surgery, improvement in clinically relevant scores comparing with baseline was observed: HOOS score increased after surgery in all its subcategories (from 32.25 ± 14.07% up to 91.91 ± 9.13%) as well as SF12 which increased by more than 18 percentage points.On clinical assessment, the range of motion (ROM) was restored at follow-up, 1 patient (1.7%) showed a squeaking hip and 2 (3.4%) reported leg-length discrepancy. Neutral stem positioning was achieved in 58 hips and heterotopic ossifications occurred in 10 hips (16%).ConclusionsThe current study reports good clinical and radiological outcomes following NANOS® short-stem hip implant at minimum 10 years-follow-up. Since the high rate of stem survivorship, the low complication rate demonstrated and the overall patient satisfaction, our results suggest NANOS® neck-preserving prostheses should be considered as a valid alternative to standard implants.

Efficient coarse registration method using translation- and rotation-invariant local descriptors towards fully automated forest inventory

In this paper, we present a simple, efficient, and robust algorithm for 2D coarse registration of two point clouds. In the proposed algorithm, the locations of some distinct objects are detected from the point cloud data, and a rotation- and translation-invariant feature descriptor vector is computed for each of the detected objects based on the relative locations of the neighboring objects. Subsequently, the feature descriptors obtained for the different point clouds are compared against one another by using the Euclidean distance in the feature space as the similarity criterion. By using the nearest neighbor distance ratio, the most promising matching object pairs are found and further used to fit the optimal Euclidean transformation between the two point clouds. Importantly, the time complexity of the proposed algorithm scales quadratically in the number of objects detected from the point clouds. We demonstrate the proposed algorithm in the context of forest inventory by performing coarse registration between terrestrial and airborne point clouds. To this end, we use trees as the objects and perform the coarse registration by using no other information than the locations of the detected trees. We evaluate the performance of the algorithm using both simulations and three test sites located in a boreal forest. We show that the algorithm is fast and performs well for a large range of stem densities and for test sites with up to 10 ​000 trees. Additionally, we show that the algorithm works reliably even in the case of moderate errors in the tree locations, commission and omission errors in the tree detection, and partial overlap of the data sets. We also demonstrate that additional tree attributes can be incorporated into the proposed feature descriptor to improve the robustness of the registration algorithm provided that reliable information of these additional tree attributes is available. Furthermore, we show that the registration accuracy between the terrestrial and airborne point clouds can be significantly improved if stem positions estimated from the terrestrial data are matched to stem positions obtained from the airborne data instead of matching them to tree top positions estimated from the airborne data. Even though the 2D coarse registration algorithm is demonstrated in the context of forestry, the algorithm is not restricted to forest data and it may potentially be utilized in other applications, in which efficient 2D point set registration is needed.

The impact of functional combined anteversion on hip range of motion: a new optimal zone to reduce risk of impingement in total hip arthroplasty.

AimsPelvic tilt (PT) can significantly change the functional orientation of the acetabular component and may differ markedly between patients undergoing total hip arthroplasty (THA). Patients with stiff spines who have little change in PT are considered at high risk for instability following THA. Femoral component position also contributes to the limits of impingement-free range of motion (ROM), but has been less studied. Little is known about the impact of combined anteversion on risk of impingement with changing pelvic position.MethodsWe used a virtual hip ROM (vROM) tool to investigate whether there is an ideal functional combined anteversion for reduced risk of hip impingement. We collected PT information from functional lateral radiographs (standing and sitting) and a supine CT scan, which was then input into the vROM tool. We developed a novel vROM scoring system, considering both seated flexion and standing extension manoeuvres, to quantify whether hips had limited ROM and then correlated the vROM score to component position.ResultsThe vast majority of THA planned with standing combined anteversion between 30° to 50° and sitting combined anteversion between 45° to 65° had a vROM score > 99%, while the majority of vROM scores less than 99% were outside of this zone. The range of PT in supine, standing, and sitting positions varied widely between patients. Patients who had little change in PT from standing to sitting positions had decreased hip vROM.ConclusionIt has been shown previously that an individual’s unique spinopelvic alignment influences functional cup anteversion. But functional combined anteversion, which also considers stem position, should be used to identify an ideal THA position for impingement-free ROM. We found a functional combined anteversion zone for THA that may be used moving forward to place total hip components.Cite this article: Bone Jt Open 2021;2(10):834–841.

High varus stem alignment in short-stem total hip arthroplasty: a risk for reconstruction of femoro-acetabular offset, leg length discrepancy and stem undersizing?

PurposeShort stems are increasingly used in in total hip arthroplasty (THA) because of advantages in bone and soft tissue preservation and reconstruction of hip geometry. Short stems can be inserted in a more varus position compared to conventional straight stems. This poses the risk of final varus misplacement of the femoral component, which is not intended in all femoral short stems.MethodsWe wanted to evaluate the effect of a high varus stem positioning in MIS THA on hip offset, leg length and femoral canal fill index. A series of 1052 consecutive THAs with a singular cementless femoral short stem and press-fit cup was retrospectively screened for inclusion. One hundred six patients with unilateral THA and a contralateral healthy hip met the inclusion criteria. Measurements were carried out on preoperative and 3 months anterior–posterior postoperative radiographs. Patients were divided into Group A (varus stem alignment ≤ 3°) and Group B (varus stem alignment > 3°).ResultsHip offset (HO) increased significantly in Group B by 4 mm (p = 0.013). No influence on leg length difference was detected in both groups. Preoperative CCD angle was significantly lower in Group B (p < 0.001). Canal Fill Indices (CFI) were significantly lower in Group B (CFI I: p < 0.001; CFI II p = 0.003; CF III p = 0.002).ConclusionHigh varus stem alignment > 3° leads to a statistically significant but minor increase in HO and poses the risk of stem undersizing. A preoperatively low genuine CCD angle pose a risk for varus stem positioning.

Effect of Stem Positioning on Biomechanical Performance of a Novel Cementless Short-Stem Canine Total Hip Implant.

The aim of this study was to evaluate the effect of stem positioning on the biomechanical performance of a novel, collared, short-stem total hip implant under compression and torsion ex vivo. Six canine cadaveric femurs were implanted with a collared short-stem femoral implant. Canal flare index (CFI), stem angle, absolute and relative cut heights and relative size were measured radiographically and used as independent variables. Biomechanical performance of the construct was evaluated using physiologic loading (loading) and supraphysiologic loading (failure) protocols. During loading protocols, compressive stiffness was influenced by absolute cut height (p = 0.018). During failure protocols, peak torque was influenced by CFI (p = 0.004) and craniocaudal relative size (p = 0.005). Peak load and torsional stiffness were not impacted by any of the radiographic variables (p > 0.05). Three of six femurs developed longitudinal fractures originating at the medial calcar at the time of failure. The biomechanical performance of the collared short-stem implant was positively impacted by preserving more of the femoral neck, having a higher CFI and using a smaller implant size relative to the femoral neck isthmus.

CORR Insights®: A Modeling Study of a Patient-specific Safe Zone for THA: Calculation, Validation, and Key Factors Based on Standing and Sitting Sagittal Pelvic Tilt.

CORR Insights®: A Modeling Study of a Patient-specific Safe Zone for THA: Calculation, Validation, and Key Factors Based on Standing and Sitting Sagittal Pelvic Tilt.

20 YEARS OF RSP – THE 5 MOST IMPORTANT LESSONS I’VE LEARNED

Introduction and aim This paper reports on 5 key aspects to consider when planning a successful RSA procedure, including patient selection, glenosphere positioning, glenoid fixation, humeral fixation, and soft tissue management/tensioning. Material, methods, results, and discussion Key in patient selection for RSA is understanding the relationship between indications, outcomes, patient mental state, and their expectations. When placing a glenosphere, prioritize sound principles of shoulder kinematics but always consider bone preservation and ease of placement. Glenoid fixation must take advantage of structural features of individual implant designs while factoring in specific-bone morphology/morphometry to optimize the resultant glenohumeral loading. For the stem, fixation prioritizes press-fit where bone quality and quantity permit. Always aim to achieve anatomical pivot point restoration with planning for stem position and avoid distalization that may lead to nerve injury and scapular spine fractures. Conclusions To increase the chances of a successful RSA procedure, a surgeon should emphasize picking the right patient, placing the implant in an optimum position, ensuring adequate glenoid and humeral fixation, and correctly balancing soft tissues. Keywords: reverse shoulder arthroplasty, surgeon education, patient optimization

Cementless total hip arthroplasty with anatomic-shaped implants. Does the minimal invasive anterolateral technique influence the stem position or subsidence in contrast to the standard lateral approach?

Using cementless stems in total hip arthroplasty (THA) has revealed variable results. The risk for early aseptic loosening has been associated to certain surgical approaches and implant designs. This study determines misalignment of collared/collarless stems and the rate of subsidence using the anterolateral approach (ALA) compared to implantations via the direct lateral approach (DLA). One hundred consecutive patients underwent primary unilateral THA using the minimal invasive ALA in the lateral decubitus position and were compared to another cohort of 100 patients, treated trough the DLA. Clinical results were noted preoperatively and after 1year using the WOMAC score. The radiographic evaluation included the stem alignment and subsidence. Overall, all patients improved in WOMAC from 48.9 points (± 11.4; 21.0-82.0) to 3.1 (± 3.2; 0.0-22.0) (p < 0.001). For DLA and ALA, the stem was placed on average 2.5° in varus to the femoral axis. The mean alignment was about 0.5° more valgus for collarless stems. The mean subsidence in the DLA group was about 0.3mm higher compared to the ALA group. With collarless implants, the subsidence was about 1.0mm higher compared to collared implants (p < 0.05), especially in patients with Dorr type B femurs. Changing from the DLA to the minimal invasive ALA did not significantly affect the implant position. Misalignment and a potential risk for early aseptic loosening could not be seen. The use of a collared cementless stem seems safe and is likely to prevent a relevant subsidence, even more so in Dorr type B femurs. III.

Acoustic Properties of Musanga Cecrepoides Wood Samples Obtained from Different Stem Positions in Niger Delta Region of Nigeria

Musanga cecrepoides is an interesting wood species due to its diverse utility ranging from medicine, shade, ornaments etc. Research has been conducted on other prop-erties of this wood species with little information about its acoustic properties. Thus, the evaluation of its acoustic properties is pertinent so as to increase the in-formation bank of its properties. This study assessed the acoustic properties of Musanga cecrepoides wood obtained from selected states and locations in the Niger Delta region of Nigerian viz a vis, Rivers, Bayelsa and Delta States. Test samples were collected from different stem positions axially (top, middle, base) and radi-ally (inner and outer) and analyzed using the statistical software package IBM SPSS Statistics, Version 23 (IBM Corporation, New York, USA). Analysis of variance (ANOVA) was performed at 5% level of significance to determine whether the as-sessed acoustic properties were significantly different among different stem posi-tions. Results showed that sound frequency (f) at top wood (3061.71 Hz) and outer wood (3096.06 Hz) had significantly higher resonance frequency compared with the bottom wood (2768.01 Hz) and inner wood (2349.54 Hz) respectively. The mean sound velocity (v) at both the axial (2069.59 m/s) and radial (1905.96 m/s) stem po-sitions fell short of the estimated mean v of other wood species when compared. However, such result is suitable for other acoustic purposes with moderate sound velocity. The sound radiation coefficient (R) values was highest at the bottom (4.18) axially and outer (4.13) radially when compared to other stem orientations of the wood. Whereas axially at the top (1561815.86 Kg/(m2s)) and radially at the outer position (1558292.53 Kg/(m2s) Sound Impedance (z) was highest when compared with other stem positions of the Musanga cecrepoides wood.

Hip implants can restore anatomical and medialized rotation centres in most cases : a 3D templating study comparing four implantation strategies.

AimsHip arthroplasty does not always restore normal anatomy. This is due to inaccurate surgery or lack of stem sizes. We evaluated the aptitude of four total hip arthroplasty systems to restore an anatomical and medialized hip rotation centre.MethodsUsing 3D templating software in 49 CT scans of non-deformed femora, we virtually implanted: 1) small uncemented calcar-guided stems with two offset options (Optimys, Mathys), 2) uncemented straight stems with two offset options (Summit, DePuy Synthes), 3) cemented undersized stems (Exeter philosophy) with three offset options (CPT, ZimmerBiomet), and 4) cemented line-to-line stems (Kerboul philosophy) with proportional offsets (Centris, Mathys). We measured the distance between the templated and the anatomical and 5 mm medialized hip rotation centre.ResultsBoth rotation centres could be restored within 5 mm in 94% and 92% of cases, respectively. The cemented undersized stem performed best, combining freedom of stem positioning and a large offset range. The uncemented straight stem performed well because of its large and well-chosen offset range, and despite the need for cortical bone contact limiting stem positioning. The cemented line-to-line stem performed less well due to a small range of sizes and offsets. The uncemented calcar-guided stem performed worst, despite 24 sizes and a large and well-chosen offset range. This was attributed to the calcar curvature restricting the stem insertion depth along the femoral axis.ConclusionIn the majority of non-deformed femora, leg length, offset, and anteversion can be restored accurately with non-modular stems during 3D templating. Failure to restore hip biomechanics is mostly due to surgical inaccuracy. Small calcar guided stems offer no advantage to restore hip biomechanics compared to more traditional designs.Cite this article: Bone Jt Open 2021;2(7):476–485.

Feeling the Beat in an African Tone Language: Rhythmic Mapping Between Language and Music

Text-setting patterns in music have served as a key data source in the development of theories of prosody and rhythm in stress-based languages, but have been explored less from a rhythmic perspective in the realm of tone languages. African tone languages have been especially under-studied in terms of rhythmic patterns in text-setting, likely in large part due to the ill-understood status of metrical structure and prosodic prominence asymmetries in many of these languages. Here, we explore how language is mapped to rhythmic structure in traditional folksongs sung in Medʉmba, a Grassfields Bantu language spoken in Cameroon. We show that, despite complex and varying rhythmic structures within and across songs, correspondences emerge between musical rhythm and linguistic structure at the level of stem position, tone, and prosodic structure. Our results reinforce the notion that metrical prominence asymmetries are present in African tone languages, and that they play an important coordinative role in music and movement.

Sampling forests with terrestrial laser scanning.

Background and AimsTerrestrial laser scanners (TLSs) have successfully captured various properties of individual trees and have potential to further increase the quality and efficiency of forest surveys. However, TLSs are limited to line of sight observations, and forests are complex structural environments that can occlude TLS beams and thereby cause incomplete TLS samples. We evaluate the prevalence and sources of occlusion that limit line of sight to forest stems for TLS scans, assess the impacts of TLS sample incompleteness, and evaluate sampling strategies and data analysis techniques aimed at improving sample quality and representativeness.MethodsWe use a large number of TLS scans (761), taken across a 255 650-m2 area of forest with detailed field survey data: the Harvard Forest Global Earth Observatory (ForestGEO) (MA, USA). Sets of TLS returns are matched to stem positions in the field surveys to derive TLS-observed stem sets, which are compared with two additional stem sets derived solely from the field survey data: a set of stems within a fixed range from the TLS and a set of stems based on 2-D modelling of line of sight. Stem counts and densities are compared between the stem sets, and four alternative derivations of area to correct stem densities for the effects of occlusion are evaluated. Representation of diameter at breast height and species, drawn from the field survey data, are also compared between the stem sets.Key ResultsOcclusion from non-stem sources was the major influence on TLS line of sight. Transect and point TLS samples demonstrated better representativeness of some stem properties than did plots. Deriving sampled area from TLS scans improved estimates of stem density.ConclusionsTLS sampling efforts should consider alternative sampling strategies and move towards in-progress assessment of sample quality and dynamic adaptation of sampling.

CORR Insights®: The External Obturator Footprint Is a Usable, Accurate, and Reliable Landmark for Stem Depth in Direct Anterior THA.

CORR Insights®: The External Obturator Footprint Is a Usable, Accurate, and Reliable Landmark for Stem Depth in Direct Anterior THA.