This study aims to investigate the antibacterial activities of essential oils isolated from various parts (buds, leaves and stems) of Eugenia caryophylata. The essentials oils were distillated by steam distillation, and the isolated was analyzed by GC and GC-MS. Five components of each sample of oils (buds, leaves and stems) were identified. Main component in the bud are eugenol (75.30%), eugenyl acetate (20.93%) and β-caryophyllene (3.00%) and eugenol (82.97%), β-caryophyllene (12.84%) in leaf oil, while in stem oil eugenol (97.75%). The oils were tested for in vitro antimicrobial activities against Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis), the Gram-negative bacteria (Escherichia coli and Salmonella typhimurium). The essential oils of bud, leaf and stem oil show that antibacterial activity against Gram-positive and Gram-negative bacteria.