Eumetopias Research Articles

Dietary niche overlap and prey consumption for the Steller sea lion (Eumetopias jubatus) and California sea lion (Zalophus californianus) in northwest Washington during 2010-2013: suppl. table 1

Dietary niche overlap and prey consumption for the Steller sea lion (Eumetopias jubatus) and California sea lion (Zalophus californianus) in northwest Washington during 2010-2013: suppl. table 1

An Old Pathogen in a New Environment–Implications of Coxiella burnetii in Australian Fur Seals (Arctocephalus pusillus doriferus)

The Australian fur seal (Arctocephalus pusillus doriferus) has experienced a slower than expected population recovery since the end of the commercial sealing era, with a high third trimester abortion rate. There is currently no known proximate cause. Coxiella burnetii (Cb) is a well-known cause of abortion in domestic and wildlife species and an important zoonotic pathogen. It has been recorded from a small number of northern hemisphere marine mammals and may be a potential contributory factor to decreasing populations of northern fur seals (Callorhinus ursinus) and Steller sealions (Eumetopias jubatus). It has not been recorded from marine mammals in the southern hemisphere but is well documented in ruminants and wildlife in Australia as a cause of reproductive failure. Third trimester aborted fetuses (n = 46) and full-term placentas (n = 66) from Australian fur seals, were collected on Kanowna Island and Seal Rocks in Bass Strait, south-eastern Australia. Utilizing routine hematoxylin and eosin histopathology, Cb immunohistochemistry and two different qPCR targets–htpAB and com1, Cb was identified. Routine histopathology and immunohistochemistry were insensitive for the detection of Cb. The detected Cb prevalence ranged from 10.6% for com1 up to 40.9% with htpAB. Coxiella burnetti was readily detected in full-term placentas but in aborted fetal material only in a single placenta associated with a still birth. The exact significance is currently unclear, but this highlights that Cb is present in Australian fur seals, breeding in Bass Strait. Bass Strait is in one of the world’s fastest warming oceanic regions and marine mammals breeding in the area are likely to be key indicators of marine ecosystem stressors. This first description of Cb in a marine mammal from the southern hemisphere, highlights the need to further investigate the potential risks this pathogen poses to Australian fur seals and sympatric marine mammals. Additionally, it is important to determine the zoonotic risk of this pathogen to persons working with, and in proximity of, Australian fur seal breeding colonies.

Anatomical features of ossa cordis in the Steller sea lion

Irregular triangular cartilage or bone fragments are sometimes found in the fibrous triangle of the heart. Ossa cordis and/or cartilago cordis has been demonstrated in various terrestrial animal species. Regarding marine mammals, sperm whales lack heart bones, and there have been no studies on bones or cartilage in pinniped hearts. Therefore, we examined the ossa cordis and/or cartilago cordis of the Steller sea lion. Eleven Steller sea lion hearts were examined morphologically and histologically. Before dissection, some hearts were imaged by CT to confirm the presence of ossa cordis or cartilago cordis. As a result, ossa cordis-like fragments were confirmed in four adults and one pup. All of the fragments were found at the right fiber triangle, and one adult had ossified tissue, including adipose tissue in the bone marrow cavity. The ossa cordis probably support the aorta because they surround the aorta as in other terrestrial animals. Steller sea lions can dive to a few hundred meters, but they need to rest on land frequently. Hence, their ossa cordis help maintain heart function during the tachycardia that occurs upon repeated surfacing and movements on land after diving in water.

Recent divergent changes in Alaskan pinniped trophic position detected using compound-specific stable isotope analysis

Over the past century, Alaskan pinnipeds have experienced dramatic changes in abundance, but these changes have been highly variable across species and regions. In recent decades, changes in atmospheric forcing and sea surface temperature have been particularly pronounced in the Gulf of Alaska and eastern Bering Sea, impacting the food webs in which Alaskan pinnipeds forage. We used compound-specific stable isotope analysis of nitrogen in amino acids to estimate historic and modern trophic positions of harbor seals Phoca vitulina and Steller sea lions Eumetopias jubatus in the Gulf of Alaska and Bristol Bay. We applied a Bayesian hierarchical framework to determine whether shared trends through time exist across pinnipeds (classified by region and species) on decadal scales. Model results identified both shared trends through time and classification-specific decadal changes in pinniped trophic position. The largest change in trophic position occurred in the 2000s and 2010s and was observed in both Steller sea lions (median: 2.8) and harbor seals (median: 3.1) in the Gulf of Alaska, but not harbor seals in Bristol Bay or Iliamna Lake. Divergent trophic position patterns in the 2000s were identified in the western stock of Steller sea lions, which increased in trophic position, and sympatric harbor seals in the northern Gulf of Alaska, which decreased in trophic position. Our results indicate that these species have been experiencing unique food web conditions in recent decades in the Gulf of Alaska, likely in response to recent climate-induced ecological change in the region.

СИНАНТРОПИЗАЦИЯ СИВУЧА (EUMETOPIAS JUBATUS, SCHREBER, 1776) НА КАМЧАТКЕ В ЧЕРТЕ Г. ПЕТРОПАВЛОВСКА-КАМЧАТСКОГО (ИТОГИ 20-ЛЕТНЕГО МОНИТОРИНГА)

The problem of adaptation of the Steller's northern sea lion (Eumetopias jubatus, Schreber, 1776) to the coastal water area adjacent to the town of Petropavlovsk-Kamchatsky is presented in the article. There are two stages in the sea lion synanthropization. The first stage is associated with rookeries formation, foraging near vessels and in the bay in area, in the usual habitat of these animals, in seawater. The second stage is connected with pioneering a new habitat and feeding on land. During sea lion adaptation to new conditions over a 20-year peri-od, the fear of humans noticeably decreased in animals, new behavioural traits such as cadging appeared. The group of sea lions wintering in Petropavlovsk-Kamchatsky during the 20-year period was characterized by sta-tistically significant fluctuations in numbers by year and season. The further coexistence of wintering animals depends on the dynamics of the influence of both natural and anthropogenic factors.

One Health, Spatial, and Cumulative Organochlorines and Mercury Exposure Assessment in Steller Sea Lions of Alaska: Emphasizing Pups

One Health, Spatial, and Cumulative Organochlorines and Mercury Exposure Assessment in Steller Sea Lions of Alaska: Emphasizing Pups

Steller sea lion diet in the Eastern Kamchatka

We studied the diet of Steller sea lion (Eumetopias jubatus) on the eastern coast of Kamchatka based on the remains of undigested food parts found in feces at terrestrial sites near Cape Kozlova (rookery) and Cape Kekurny (haulout) in the summer. A total of 276 fecal samples were analyzed from 2004 to 2008. Forty eight food items of 17 fish families and one family of cephalopod mollusks were identified. Steller sea lion diet was diverse yet dominated by only several species. The pollock Gadus chalcogrammus, Atka mackerel Pleurogrammus monopterygius, Pacific sand lance Ammodytes hexapterus and Hemilepidotus sp. were dominant by frequency of occurrence in decreasing order at both sites. Differences were found in the Steller sea lion diet in the studied sites. Prey diversity at Kekurny Cape within scat samples ranged from one to twelve objects within the median of four prey item per a sample. The median of prey diversity at Kozlov Cape within scat samples was two food objects within a range of one to nine prey types per sample. Of the 37 scats (24,5 %) collected at Kozlov Cape that contained a single prey item, 75,7% contained Atka mackerel. Among scats collected at Kekurny Cape that contained a single prey species (n=10, 8,0 %), 50,0 % contained Atka mackerel and 40,0 % pollock. In contrast to the high diet diversity at Kekurny Cape haulout, breeding Steller sea lions at Kozlova Cape rookery fed on a narrow set of prey items. Interannual changes in the diet structure at each site were not statistically significant.

ЛЕТНЕЕ ПИТАНИЕ СИВУЧА В ВОДАХ ДАЛЬНЕГО ВОСТОКА РОССИИ В 2004–2008 ГОДАХ

The diet of Steller sea lion Eumetopias jubatus was studied based on undigested food parts found in feces collected in the summer season on 20 rookeries in the Russian Far East. A total of 915 samples were analyzed between 2004 and 2008, in which 65 food items were identified. The frequency of occurrence of 11 diet items was greater than 5%, including Atka mackerel Pleurogrammus monopterygius (64.8%), Pollock Theragra chalcogramma (44.3%), Pacific salmon Salmonidae (20.5%), Irish lord Hemilepidotus sp. (20.4%), Pacific sand lance Ammodytes hexapterus (15.8%), Pacific herring Clupea pallasi (9.5%), Squid and Octopus Cephalopoda (8.4%), Pacific cod Gadus macrocephalus (5, 9%), Pacific sandfish Trichodon trichodon (5.9%), Flatfishes Pleuronectidae (5.8%), Threespine stickleback Gasterosteus aculaeatus (4.9%). Three regions with different diets were identified – the Sea of Okhotsk, the Kuril Islands, and Kamchatka with the Commander Islands. In the Sea of Okhotsk, Pollock and Herring (83.6% by frequency of occurrence) dominated among undigested food remains in feces. The diet at the Kuril Islands rookeries was variable within the region. The general pattern was a low diversity of diet at each Kuril Island sites and predominance of 1-3 food items, probably the most accessible near the sites (Pollock, Squid and Octopus, Atka mackerel, Pacific salmon). The diet pattern in Kamchatka and the Commander Islands in the summer season was characterized by a high diet diversity. In contrast to other regions, the diet includes in high proportion small non-commercial species – Sand lance, Irish lord, Pacific sandfish, Flatfishes, Threespine stickleback and others. The regional patterns of sea lion diets show the spatial distribution and areas of abundant biomass of the main food items. The diet patterns of sea lions from rookeries differed from haulouts in having a higher content of abundant food items. Long-term changes in diet structure were not statistically significant.

Steller Sea Lion (Eumetopias jubatus) Response to Non-lethal Hazing at Bonneville Dam

Protected Steller sea lions (Eumetopias jubatus) aggregate at Bonneville Dam on the Columbia River and prey upon multiple species of endangered salmon ascending the river. Hazing is a non-lethal activity designed to repel sea lions that includes aversive auditory and physical stimuli to deter animals from an area and has been employed with sea lion—fisheries interactions for more than 40 years but sea lion responses to hazing through time is not well-documented. We observed the behavior of Steller sea lions in periods with and without hazing during two spring Chinook salmon passage seasons to evaluate: (1) what effect hazing had on the number of animals present and their foraging behavior, and (2) whether they habituated to hazing. We found that hazing temporarily reduced the number of Steller sea lions, but only when actively hazed. During hazing, Steller sea lions were more likely to move away from hazers on the dam, decreased their foraging, and increased their time investigating the environment. However, these effects were temporary; their behavior returned to initial observation levels once hazing ceased. Furthermore, their responsiveness to hazing declined throughout the season, indicating habituation and raising concern for the application and long-term efficacy of hazing in managing predation on endangered salmon.

California sea lion population decline at the southern limit of its distribution during warm regimes in the Pacific Ocean

We evaluated the effect of regime shifts on the population dynamics of the California sea lion colony of Santa Margarita Island from 1979–2020. This colony is found at the southern extreme of the California sea lion’s range in the North Pacific Ocean in the temperate–tropical​ transition zone characterized by high primary productivity where oceanic and lagoon environments converge. It is largely isolated from other colonies. We assessed the influence of sea surface temperature and net primary productivity on sea lion population dynamics using historical abundance data taken from the literature and collected in the field (1979–2015). We also performed a Population Viability Analysis to determine the population trend and predict the probability that the colony could go extinct. Anomalies of sea surface temperature (1981–2020) and net primary productivity (2002–2020) were analyzed. Warm and cold periods were determined using the Regime Shift Detector. Colony size decreased at a rate of 4% per year, a decrease of ∼75% over 36 years. Four regimes (two cold and two warm) were identified in relation to the sea surface temperature and two regimes (one cold and one warm) in relation to the net primary productivity. The number of births decreased during strong El Niño events under both warm and cold regimes. During warm regimes, a lower abundance of sea lions was observed compared to the cold regimes that preceded them. Population declines due to regime shifts have also been observed in several species of pinnipeds that inhabit the North Pacific (e.g., the Steller sea lion). The Santa Margarita Island breeding colony meets the IUCN’s quantitative criteria for “Vulnerable” or “Endangered”. Our results highlight the importance of a colony-level approach in understanding how populations respond to changes in environmental conditions.

Pinnipeds and salmon farming: Threats, conflicts and challenges to co‐existence after 50 years of industrial growth and expansion

AbstractIn this review, we summarize and discuss the main interactions affecting salmon farming and pinniped populations worldwide. Out of seven pinnipeds commonly engaged in these interactions, only one, Eumetopias jubatus, is currently listed, as ‘Near Threatened’, by the IUCN. This scenario may change as the salmon farming industry is expanding to higher latitudes and climate change is threatening many pinniped populations. Pinniped attacks cause productive and economic losses, equivalent to 1%–12% of gross production costs, due to predation, induced escapes and implementing deterrent measures. Other potential negative effects on the industry remain unassessed: lower fish growth, immunosuppression, diseases transmission, damaging infrastructure and injuring farmworkers. Main direct threats to pinnipeds include intended and unintended killing, which lack independent quantifications and have generated a growing number of ethical, economic and socio‐political concerns, as well as trade regulations affecting the industry. Physical perturbation, pollution, nutrification and/or prey subsidies related to this industry may induce behavioural, trophic and social changes, as well as breeding and/or foraging habitat loss/change in impacted areas. Despite their relevance, the magnitude and consequences of these potential impacts have been poorly evaluated and documented, precluding us from assembling accurate assessments on most interactions. Hence, we recommend focusing further research on assessing the effects of these interactions and make a call for implementing and maintaining reliable and independent records of: (i) intended and unintended killings and (ii) demography of affected populations. Implementing an ecosystem approach may be key to reduce negative impacts of salmon farming on pinnipeds and coastal ecosystems.

Species density models from opportunistic citizen science data

Abstract With the advent of technology for data gathering and storage, opportunistic citizen science data are proliferating. Species distribution models (SDMs) aim to use species occurrence or abundance for ecological insights, prediction and management. We analysed a massive opportunistic dataset with over 100,000 records of incidental shipboard observations of marine mammals. Our overall goal was to create maps of species density from massive opportunistic data by using spatial regression for count data with an effort offset. We illustrate the method with two marine mammals in the Gulf of Alaska and Bering Sea. We counted the total number of animals in 11,424 hexagons based on presence‐only data. To decrease bias, we first estimated a spatial density surface for ship‐days, which was our proxy variable for effort. We used spatial considerations to create pseudo‐absences, and left some hexagons as missing values. Next, we created SDMs that used modelled effort to create pseudo‐absences, and included the effort surface as an offset in a second stage analysis of two example species, northern fur seals and Steller sea lions. For both effort and species counts, we used spatial count regression with random effects that had a multivariate normal distribution with a conditional autoregressive (CAR) covariance matrix, providing 2.5 million Markov chain Monte Carlo (MCMC) samples (1,000 were retained) from the posterior distribution. We used a novel MCMC scheme that maintained sparse precision matrices for observed and missing data when batch sampling from the multivariate normal distribution. We also used a truncated normal distribution to stabilize estimates, and used a look‐up table for sampling the autocorrelation parameter. These innovations allowed us to draw several million samples in just a few hours. From the posterior distributions of the SDMs, we computed two functions of interest. We normalized the SDMs and then applied an overall abundance estimate obtained from the literature to derive spatially explicit abundance estimates, especially within subsetted areas. We also created ‘certain hotspots’ that scaled local abundance by standard deviation and using thresholds. Hexagons with values above a threshold were deemed as hotspots with enough evidence to be certain about them.

Counting sea lions and elephants from aerial photography using deep learning with density maps

BackgroundThe ability to automatically count animals is important to design appropriate environmental policies and to monitor their populations in relation to biodiversity and maintain balance among species. Out of all living mammals on Earth, 60% are livestock, 36% humans, and only 4% are animals that live in the wild. In a relatively short period, development of human civilization caused a loss of 83% of wildlife and 50% of plants. The rate of species extinction is accelerating. Traditional wildlife surveys provide rough population estimates. However, emerging technologies, such as aerial photography, allow to perform large-scale surveys in a short period of time with high accuracy. In this paper, we propose the use of computer vision, through deep learning (DL) architecture, together with aerial photography and density maps, to count the population of Steller sea lions and African elephants with high precision.ResultsWe have trained two deep learning models, a basic UNet without any feature extractor (Model-1) and another with the EfficientNet-B5 feature extractor (Model-2). We measured the model’s prediction accuracy, using Root Mean Square Error (RMSE) for the predicted and actual animal count. The results showed an RMSE of 1.88 and 0.60 to count Steller sea lions and African elephants, respectively, regardless of complex background, different illumination conditions, heavy overlapping and occlusion of the animals.ConclusionsOur proposed solution performed very well in the counting prediction problem, with relatively low training parameters and minimum annotation. The approach adopted, combining DL and density maps, provided better results than state-of-art deep learning models used for counting, indicating that the proposed method has the potential to be used more widely in large-scale wildlife surveying projects and initiatives.

Steller Sea Lion (Eumetopias jubatus) at Tuleny Island, Russia in Autumn 2018: Abundance, Composition, and Entanglement.

The Steller sea lion (SSL, Eumetopias jubatus) inhabits the North Pacific Ocean off both the North American and Asian coasts. The abundance of the species in Asia declined by more than half in the second part of the 20th century. Decline recurred in the second decade of the 21th century after a short period of restoration. In contrast with the total dynamics of SSL in Asia, the reproductive aggregation on Tuleny I. (Sea of Okhotsk) has been growing almost continuously since the beginning of its formation in the late 1980s. Long-term monitoring of SSL at Tuleny I. always covered only summer reproductive seasons. We surveyed Tuleny I. in October 2018, and counted 1058 non-pup sea lions and 396 pups. The majority of tagged animals encountered at the rookery were of local origin. About one-third of the summer non-pup sea lions' number could remain at the rookery until the middle of October, which coincides with the seasonal appearance of sea lions off the coast of Japan. The sex-age structure was characterized by total absence of adult males and reduced proportion of subadult males and juveniles. It contrasts with autumn behavior of SSLs in the rookery of the northern Sea of Okhotsk. We observed that 0.7% of the inspected sea lions were entangled in marine debris. The proportion of entangled animals is lower in comparison with that of the whole population of Tuleny I. due to reduction in the ratios of males and juveniles, which entangle in foreign objects more often than adult females.

CARDIAC EXAMINATIONS OF ANESTHETIZED STELLER SEA LIONS (EUMETOPIAS JUBATUS), NORTHERN FUR SEALS (CALLORHINUS URSINUS), AND A WALRUS (ODOBENUS ROSMARUS).

Pinniped hearts have been well described via dissection, but in vivo measurements of cardiac structure, function, and electrophysiology are lacking. Electrocardiograms (ECGs) were recorded under anesthesia from eight Steller sea lions (Eumetopias jubatus), five northern fur seals (Callorhinus ursinus), and one walrus (Odobenus rosmarus) to investigate cardiac electrophysiology in pinnipeds. In addition, echocardiograms were performed on all eight anesthetized Steller sea lions to evaluate in vivo cardiac structure and function. Measured and calculated ECG parameters included P-wave, PQ, QRS, and QT interval durations, P-, R-, and T-wave amplitudes, P- and T-wave polarities, and the mean electrical axis (MEA). Measured and calculated echocardiographic parameters included left ventricular internal diameter, interventricular septum thickness, and left ventricular posterior wall thickness in systole and diastole (using M-mode), left atrium and aortic root dimensions (using 2D), and maximum aortic and pulmonary flow velocities (using pulsed-wave spectral Doppler). ECG measurements were similar to those reported for other pinniped species, but there was considerable variation in the MEAs of Steller sea lions and northern fur seals. Echocardiographic measurements were similar to those reported for southern sea lions (Otaria flavenscens), including five out of eight Steller sea lions having a left atrial to aortic root ratio <1, which may indicate that they have an enlarged aortic root compared to awake terrestrial mammals. Isoflurane anesthesia likely affected some of the measurements as evidenced by the reduced fractional shortening found in Steller sea lions compared to awake terrestrial mammals. The values reported are useful reference points for assessing cardiac health in pinnipeds under human care.

The effect of food restriction on growth rates in Steller sea lions,Eumetopias jubatus

The effect of food restriction on growth rates in Steller sea lions,<scp><i>Eumetopias jubatus</i></scp>

First Confirmed Sighting of Steller Sea Lion (Eumetopias jubatus) in China Since 1990

First Confirmed Sighting of Steller Sea Lion (Eumetopias jubatus) in China Since 1990

Organochlorine exposure and health effects in stranded Steller sea lions (Eumetopias jubatus) and Pacific harbor seals (Phoca vitulina richardii) from Oregon and southern Washington coasts

AbstractOrganochlorine (OC) pesticides and polychlorinated biphenyls (PCBs) have been detected in a variety of marine mammal species at levels associated with adverse health effects. Little is known about OC levels and impacts on health in pinnipeds with different life histories. We determined the health and levels of 18 OC pesticides and 16 PCB congeners in blubber samples from 20 Steller sea lions and 39 Pacific harbor seals stranded from Oregon and Southern Washington. The most commonly detected OC at the highest concentration was p,p′‐ dichlorodiphenyldichloroethylene (DDE). PCBs were detected in all samples as well. Hypothesis testing indicated that diseased Steller sea lions (males and females combined) had higher contaminant concentrations than healthy Steller sea lions, and diseased Pacific harbor seals had higher concentrations of total OCs than healthy animals. Differences were also noted between diseased and healthy animals when looking at individual sexes of each species. Diseased Steller sea lions had higher mean contaminant levels than diseased harbor seals and healthy Steller sea lions had higher mean contaminant concentrations than healthy Pacific harbor seals. These results show that species differences exist in both contaminant loads and sensitivity to contaminants, which may be due to differences in life histories and physiology.

Regional variations and relationships among cytokine profiles, white blood cell counts, and blood mercury concentrations in Steller sea lion (Eumetopias jubatus) pups

The Steller sea lion (SSL) population west of 144°W longitude experienced a significant population decline. While there appears to be a stable or increasing population trend in rookeries in the Gulf of Alaska (GOA) and Southeast Alaska (SEA), some rookeries within the Aleutian Islands (AI) have failed to recover. Previous studies found regional differences in whole blood total mercury concentrations ([THg]) showing more than 20% of AI pups had [THg] above critical thresholds for increased risk of immunological effects and other adverse outcomes. Measurements of immune cell-signaling proteins can be used to evaluate the immune status of marine mammals in relation to [THg]. We compared serum cytokine and chemokine concentrations in pups among regions (AI, eastern GOA, SEA), and examined associations among cytokines, chemokines, white blood cell (WBC) counts, and [THg]. Considering liver is an important target organ for mercury and immune protein synthesis we additionally examined the relationship of [THg] with liver-related enzymes serum aspartate (AST) and alanine aminotransferase (ALT). We observed regional differences in cytokine and chemokine measurements and immune protein associations. There was a positive association between total WBC counts and [THg] in AI pups, whereas a negative association between lymphocytes and [THg] in SEA pups. These findings may indicate regional variation in proliferation and differentiation of hematopoietic cells, differences in immune system development, and/or a difference in antigenic stimuli. No associations between [THg] and cytokines, chemokines, AST or ALT were found. Observed regional differences in cytokine and chemokine milieu during gestational and early development in SSL pups could lead to an imbalance in cell differentiation that could impact immunological resiliency in juvenile and adult life stages. We report concentration ranges of a suite of cytokines and chemokines which may prove to be a useful metric for ecotoxicology and risk assessment studies in SSLs and other wildlife.

Abundance Estimates of Steller Sea Lions (Eumetopias jubatus) Off the Western Coast of Hokkaido, Japan

Aerial surveys were conducted to estimate the abundance of wintering populations of Steller sea lions (SSLs; Eumetopias jubatus) in the Sea of Japan originated from Russian waters using the line transect sampling method during spring from 2005 to 2019. The survey areas covered the continental shelf off the western coast of Hokkaido. A total of 28 196 km survey effort was made through the study period under suitable sighting conditions. The numbers of SSLs in haul-out sites were counted separately. Estimations and model selection for the effective strip width were carried out using the maximum-likelihood method. The abundance estimates of SSLs with the line transect method varied annually, ranging from 284 animals (coefficient of variation [CV] = 0.732) in 2016 to 6271 (CV = 0.194) in 2014. This large variation in abundance estimates might be attributable to interannual changes in the survey coverage, spatial distribution, haul-out behaviors, and seasonal variation of wintering populations of SSLs in the waters off Hokkaido. This warrants further investigation of seasonal and annual change of distribution to adjust key information on the abundance estimates reported here for use in the conservation and management of this species.