The localized loading behavior of cold-formed stainless steel (CFSS) rectangular hollow section (RHS) members at elevated temperatures is investigated in this paper. Fifteen localized loading tests conducted at various temperatures up to 800°C were reported. The tests were carried out under the Interior-One-Flange (IOF) load case as specified in American Specification for the design of cold-formed stainless steel structural members. Tensile coupon tests were performed to obtain the material properties of the CFSS RHS at various temperatures. Detailed information on dimensions, temperature history, failure modes, load-deformation curves, localized loading capacities of specimens at various temperatures were presented. In addition, a numerical investigation was supplemented, where a total of 192 finite element (FE) analyses were undertaken after validation of developed FE model against the elevated-temperature test results. The influence of various parameters on the localized loading capacities of CFSS RHS at different temperatures under IOF load case was revealed. The obtained results were utilized to assess the applicability of the localized loading design provision for CFSS RHS under IOF load case codified in the American Specification to elevated temperature conditions. Moreover, the results were also compared with predictions based on existing design rules in literatures for cold-formed RHS at elevated temperatures. Furthermore, reliability analyses were carried out to assess the reliability levels of the assessed design methods. It is shown that the existing design rules can generally provide conservative and reliable predictions of the localized loading capacities for the CFSS RHS under IOF load case at elevated temperatures.