In this work, the tribological properties of two diamond-like carbon (DLC) films were investigated at different temperatures using water-engine oil emulsions as lubricants, considering emerging fuel systems such as hydrogen and ammonia. The results revealed that the friction coefficient of a-C film raised with the increasing water content at 80 °C. The friction coefficient of a-C:H film initially raised and then decreased with varying water fractions at different temperatures. Elevated temperature exacerbated the wear of both DLC films at high water contents. The most significant wear was observed for a-C film at 80 °C and a-C:H film at 50 °C. The a-C:H film exhibited more sensitive wear characteristics to water content than the a-C film. Surface analysis indicated that water-engine oil emulsions prevented the formation of phosphate tribo-film on steel friction pair for two DLC films but promoted the tribo-oxidation of steel pair sliding contact with a-C film, especially at higher temperatures. Distinct reactivities of two DLC films towards water molecules could affect the adsorption of lubricants on DLC surfaces, leading to their different friction and wear behavior. It suggests that the water-caused emulsification of lubricating oil will be a pivotal consideration in tribological applications of DLC films in future internal combustion engines.