In this study, the temperature influence on iron loss of non-oriented steel laminations is investigated. The iron loss variation under different flux densities, frequencies and temperatures is systematically measured and analysed by testing two typical non-oriented steel laminations, V300-35 A and V470-50 A. The iron loss variation with temperature is almost linear in the typical operating temperature range of electrical machines. Furthermore, the varying rate of iron loss with temperature varies with flux density and frequency. A coefficient which can fully consider the temperature influence is introduced to the existing iron loss model to improve the iron loss prediction accuracy. The predicted and measured results show that the temperature influence on the iron loss can be effectively considered by utilising the improved model, i.e. the prediction accuracy of the improved iron loss model remains constant, even when the temperature varies significantly. A potential simplification of this improved model is also discussed in this study.