Steam Distillation Method Research Articles

Phytochemical Screening, Chemical Constitutions, and Antibacterial Activity for three Medicinal Plants Against Multidrug-Resistant Bacteria

Due to the importance of therapeutic plants in the production and preparation of drugs, The purpose of this study is to extract three medicinal plants, including seeds of Syzygium aromaticum “S. aromaticum”, leaves of Thymus vulgaris (T. vulgaris) and Myrtus communis (M. communis) using the steam distillation method. It evaluated their ability to inhibit pathogenic and antibiotic-resistant bacteria, including Staphylococcus aureus (S.aureus), Pseudomonas aeruginosa (P. aeruginosa), and Escherichia coli (E.coli) isolated from skin ulcers. Moreover, these extracts were cured to evaluate their phytochemicals and chemical constitutions using classical methods and Gc-mass spectroscopy. The phytochemicals results showed that the Flavonoids and terpenoids were observed in all samples, while the Alkaloids didn't appear. The extracts of S. aromaticum, T. vulgaris, and M. communis showed interesting compounds during the GC-mass spectroscopy test. It showed the presence of chemical compounds with active groups that could have a significant role in bacterial treatment, such as 9-octadecenamide, Caryophyllene, and Limonene. Moreover, The results of the bacterial test showed a high effectiveness of these oils on the types of bacteria used, especially S. aureus, and E. coli, where the highest results were for Myrtus on E. coli bacteria with an inhibition diam of 56 mm, while clove oil did not show any effectiveness against S. aureus, the inhibition zone diameter is zero. In general, the effectiveness of these essential oils against Gram N. P.aeruginosa was very weak compared to the effectiveness of these essential oils on other bacterial species used in the study, where the highest diameter of inhibition of these bacteria was by clove oil and reached 15 mm.

NMR Analysis of Pulegone in Food Products

Pulegone is a monoterpene ketone found in a variety of mint species. It has been classified as possibly carcinogenic to humans (Group 2B) by the International Agency for Research on Cancer (IARC). In previous studies, pulegone in food was analyzed exclusively via GC-MS, while 1H NMR methods were limited to essential oils. The aim of this study was to develop an NMR method for the detection and quantification of pulegone in essential oils and foods. A mixture of methanol-d4/chloroform-d1 in a 1:1 ratio (v/v) was identified as the most effective solvent for separating pulegone signals. The essential oils were subjected to analysis at this solvent-mixture ratio. The extraction of pulegone was required for food analysis, and the steam distillation method proved to be more effective than the ultrasonic-assisted extraction method. The highest pulegone concentrations were identified in pennyroyal oil and muña oil, whereas lower levels were observed in other matrices, including corn mint oil and select food items. A toxicological assessment showed that the amount consumed did not exert any adverse effects on human health.

PRESERVATION POTENTIAL AND MICROBIAL GROWTH INHIBITORY EFFECT OF GALANGAL AND LEMONGRASS OILS AND EXTRACTS ON CHICKEN FILLETS

Galangal (Alpinia galanga (L.) Willd.) and lemongrass (Cymbopogon citratus (DC.) Stapf) are spices known for their antimicrobial activity and potentially developed into a natural food preservative. This study evaluated the potential development of galangal and lemongrass oils and extracts for natural food preservatives. The essential oils and water extracts of galangal and lemongrass were obtained by water and steam distillation and infusion methods, respectively. Chicken fillets were immersed in three concentrations of galangal and lemongrass oils and extracts, with sterile water as the control. The fillets were tightly closed, kept in the refrigerator for 15 days, and evaluated for their preservation potential and microbial inhibitory activity on days 3, 6, 9, 12, and 15. The chicken fillet preservation potential was evaluated by comparing the time spoilage signs started to be observable in the control and treatment groups. The percent of microbial growth inhibition (%MI) was calculated from optical density (OD) and was used as the antimicrobial activity parameter. The %MI was analyzed by two-way ANOVA at α=0.05. Treatment and storage time significantly affected %MI. Galangal extracts at 10 and 20% delayed rancid odor, off-texture, and slime formation for nine days. Microbial growth inhibitory activity was shown by galangal oil at 0.25, 1.25, and 6.25 mg/ml and galangal extract at 20%. In conclusion, galangal extract at 20% was the most promising sample potentially developed into a natural chicken fillet preservative.

Isolation and Antibacterial Activity of Sembung (Blumea balsamifera) Leaf Essential Oil L., DC

Sembung leaf essential oil is a complex mixture in the form of oily yellow liquid with a unique aroma. It contains various volatile components that have antibacterial, antifungal, antioxidant and cytotoxic activities. This study aims to determine the antibacterial activity of essential oil Blumea balsamifera L., DC against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. Isolation of essential oil using steam distillation method with 0.21% rendement. Inhibition activity was performed by test tube method with variation concentrations of essential oil were 10%, 20%, 40%, 80%, and 100% for each bacteria. Results of this study showed that the highest antibacterial activity on Escherichia coli at 80% concentration with an inhibition zone diameter of 27.53 mm, 100% concentration for Pseudomonas aeruginosa with an inhibition zone diameter 25.72 mm and 100% concentration for Staphylococcus aureus with inhibition zone diameter 27.31 mm. Essential oil from (Blumea balsamifera) L., DC leaves have a strong activity inhibition category against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus.

The Effect of Harvest Time of Sapu-Sapu Leaves (Baeckea frutescens L.) the Yield, Characteristics and Composition of Essential Oils Extached Using Steam-Hydro Distillation Method

The sapu-sapu plant (Baeckea frutescens L.) is widespread in the Bangka Belitung Islands Province, thriving in sandy areas such as beaches and highlands with less fertile soil. This study aimed to determine the optimal harvest time for sapu-sapu leaves to obtain essential oils' highest yield and most desirable characteristics. The research focused on the duck-type sapu-sapu leaves, using five variations of harvest time (coded as A1, A2, A3, A4, and A5, representing leaves harvested at 1, 2, 3, 4, and 5 months of growth, respectively). Essential oil extraction was performed using the steam distillation method. The resulting oils were then analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) to determine their chemical composition. The results showed that sample A1 (1-month growth) produced the highest essential oil yield at 0.74% (w/w). This sample also exhibited the most optimal essential oil characteristics: clear yellow color, characteristic sapu-sapu odor, warm bitter taste, solubility in 96% ethanol at 1:13, specific gravity of 0.8863 g/mL, optical rotation of (+) 10.28°, and refractive index of 1.474. GC-MS analysis revealed that the main compounds in the A1 sample were α-pinene (43.84%), β-pinene (13.56%), and 1,8-cineol (24.26%). The study concluded that while the variation in harvest time of sapu-sapu leaves did not significantly affect the yield, characteristics, or composition of the essential oils, there were slight differences in yield and color. Sample A1, representing the youngest leaves, produced the most optimal results.

Chemical composition and antimicrobial activity of Plinia rivularis essential oil against multidrug-resistant bacteria

IntroductionThe increasingly common occurrences of multidrug-resistant bacteria represent a global health threat and the discovery of new alternative remedies is necessary. The use of essential oils (EOs) in the treatment of many pathogenic bacteria is promising and several examples have already been highlighted regarding their inhibitory action. Among the botanical families, Myrtaceae stands out for presenting important species that supply bioactive products. MethodsIn this work we investigate the chemical composition of Plinia rivularis (Myrtaceae) leaf EO and its antibacterial activity against resistant and non-resistant bacteria. The EO was obtained by two methods, hydrodistillation and steam distillation. Gas chromatography-flame ionization detector (GC-FID), gas chromatograph-mass spectrometry (GC-MS), nuclear magnetic resonance spectroscopy (NMR) and Fourier transform infrared spectroscopy (FTIR) were the techniques used in the EO chemical characterization. ResultsThe EOs were obtained with yields of 0.37 and 0.16% by hydrodistillation and steam distillation methods, respectively. The analyzes revealed the EOs chemical profile showing the compounds 2H-benzocyclohepten-2-one,3,4,4a,5,6,7,8,9-octahydro-4a-methyl (synonym: 4a-methyl-3,4,4a,5,6,7,8,9-octahydro-2H-benzo[7]annulen-2-one), rosifoliol, β-eudesmol and the diterpene kaurene as main constituents. Through preparative thin-layer chromatography (PTLC), it was possible to obtain the diterpene kaurene with a content of 91%, which was also characterized by NMR. The minimum inhibitory concentrations of EO against bacteria ranged from 0.56 to 2.25 mg.ml−1 and 2.25–36 mg.ml−1 for sensitive and resistant bacteria, respectively. Also, EO showed synergistic activity with amikacin and cefotaxime against carbapenem-resistant Pseudomonas aeruginosa (KPC). ConclusionsThis is the first report on the chemical composition of P. rivularis EO. Comparison of extraction methods showed variation in yields and small changes in component content. The EO demonstrated activity against resistant and non-resistant bacteria. In addition, the synergistic effect of EO against resistant bacteria may be explored in future studies.

Effect of humic acids extracted from different organic sources and inoculation with Bacillus subtilis or Aspergillus Niger on urease activity in calcareous soil

Humic substances have received a large share of research and development as amendments to poor fertility soils that are poor in fertility and have deteriorating physical properties, since the process of extracting is a common process and easy to implement, as well as diluting these acids with water enables them to be used at economically acceptable levels over large areas. It is necessary to noticed that the properties of these acids depending on the organic source and the concentration added to the soil. A laboratory experiment was conducted at the Department of Soil Sciences and Water Resources, College of Agriculture, University of Basrah ,IRAQ to demonstrate the effect of humic substances extracted from different sources and inoculation with Bacillus subtilis or Aspergillus niger on the activity of the urease in calcareous soil. Wheat straw, alfalfa leaves, goat waste and poultry waste were composted aerobically for 3 months by filling a hole of 2×2×1.5 m by layers of such waste ,turned weekly for aeration. Each pile was extracted for humic acids (humic acid + fulvic acid) . Humic acid mixed with to soil at levels of 0, 25, and 50 L h-1. The soil was also inoculated with Bacillus subtilis or Aspergillus niger isolated from local soil at rates of 20.06×106 and 40× 103 cfu ml-1, respectively The samples were incubated at field capacity level 28±2°C for 30 days. Urease activity was estimated after 7 or 30 days by determining NH4+ released using steam distillation method . A decrease in urease activity was observed when treated with different types of humic acid compared to the control treatment. The lowest activity was obtained at humic acids extracted from poultry waste, reaching 312.0 and 188.72 µg N-NH4+g-1soil.2h-1 at incubation periods of 7 and 30 days, respectively .The results also indicated that increasing the level of humic acids significantly inhibited the activity of the enzyme. However, in soil treated with Bacillus subtilis or Aspergillus niger, an increase in urease activity was observed compared to the non-inoculation treatment. Treatment with the fungal inoculant had higher activity than the bacterial inoculum, reaching 324.5 and 297.2 µg N-NH4+ g-1 soil 2 hours-1 for both inoculants at a period of 7 days, and 178.09 and 150.31 µg N-NH4+g-1soil.2h-1at a period of 30 days, respectively.It is proposed that the inhibition of urease by humic acid addition may benefit for increasing urea fertilizer efficiency.

CHEMICAL COMPOSITION OF THE ESSENTIAL OIL OF WORMWOOD (ARTEMISIA ABSINTHIUM L.), GROWING IN THE WESTERN REGION OF AZERBAIJAN

Wormwood (Artemisia absinthium L.) is a widespread plant species in the Western region of Azerbaijan. The dependence of the composition, quantity and growth of various biologically active substances contained in the above-ground parts of wormwood on the vegetation period and climatic conditions was studied. The results of the study of the essential oil obtained by the steam distillation method from wet and dry samples of the aerial parts of bitter wormwood are presented. The yield of essential oil is determined in % in terms of the weight of air-dried raw materials. The study of the yield of essential oil was different months of the year showed that its amount varies depending on the vegetation period (yield of 0.56-0.66% by mass of fresh raw materials; yield of 0.58-0.77% by mass of dry raw materials). The composition of volatile distillates was studied by the chromatography method. In all samples, the amount of camphor component, which belongs to oxygenated terpenoid hydrocarbons, prevailed with a percentage above 35%. 25.44-34.24% phase budding in June for fresh and dry plant material; 25.14-35.21% phase mass flowering in August; it was determined between 25.67-35.20% phase the seed ripening in September.

The benefits and challenges of treating skin with natural oils.

The term natural oil refers to a fixed (non-volatile) oil of animal or plant origin. These types of oils - in contrast to essential (volatile) oils, which are obtained by steam distillation methods of plant matter - are typically obtained from plant seeds and nuts by a mechanical pressing technique or solvent extraction. The natural movement in cosmetics of the 21st century has led to renewed interest in formulating skin care products with botanical ingredients. In this article, we discuss the benefits and caveats of natural oil treatments as moisturizing agents (as occlusives and emollients) as well as their utility in wound healing and treatment of skin diseases. We also address the paradoxical behaviour of natural oils in relation to barrier function and highlight the current state of our knowledge with respect to the use of natural oils in neonatal skin care. Finally, we provide a comparison of natural oils to conventional petroleum-based oils.

Validation Study on Spectrophotometric Measurement of Ethanol from Beer and Non-Alcoholic Beer Samples Distilled by Micro Steam Distillation Method

The method validation technique for determining the ethanol percentage (v/v) in beer and non-alcoholic beer is presented in this study. The underlying idea is to use the micro water vapor method to distill the ethanol from beer and then use the spectrophotometric method, which uses the oxidation of sodium dichromate to measure the amount of ethanol. The Harmonized Guidelines for Single-Laboratory Validation of Methods of Analysis were used to validate the analytical method that we provided. The following aspects of the method were assessed: precision, recovery, linearity, measuring range, detection and quantification limitations, method detection limit, and measurement uncertainty. The following were the limits of detection (LOD) and quantification values (LOQ), and the method detection limit (MDL): ethanol, 0.04%, 0.05%, and 0.15. For both repeatability and within-laboratory reproducibility, the relative standard deviation values were less than 2.36 and 4.12%, respectively. The spiked samples had recovery rates ranging from 97% to 102%. These findings fulfilled the minimal performance standards set forth in AOAC Official Methods of Analysis Appendix F: Guidelines for Standard Method Performance Requirements. As a result, the process can be used for the regular analysis of ethanol in the beer and non-alcoholic beer under study.

A Silica Nano-Additive's Impact on the Performance and Emissions of a Diesel Engine Using DTBP Blended Low Viscous Waste Orange Peel Oil

<p>In the current study, the effect of silicon dioxide (SiO2) nano-additive mixed with Orange peel oil (OPO) as Biodiesel was investigated in relation to the operation and emission characteristics of a diesel engine. Orange peel oil was extracted from the orange Peel waste by using steam distillation method. The addition of Di-tetra-butyl-peroxide (DTBP) as a cetane booster is a novel strategy that has been attempted to improve the ignition patterns of OPO. The properties of customized fuel produced with a varying volume percentage DTBP and SiO2in 25,50 and 75 ppm in OPO were determined and compared to those of diesel. Uniform blending of nano-additives + DTBP with OPO is achieved by ultrasonication and isopropyl alcohol is used as a surfactant in order to stabilise the nano-additives. The physical and chemical characteristics of samples of pure and mixed fuel were identified and found in accordance with ASTM standards. Under varied loading conditions, the performance and emissions characteristics of various samples of fuel were studied. Addition of SiO2 nano-additive + DTBP increased the brake thermal efficiency (BTE) by 3.20-8.37% while decreasing brake specific fuel consumption (BSFC) by 4.18-11.25%. Carbon monoxide (CO), hydrocarbon (HC),nitrogen oxides (NOx) and smoke emissions were reduced by 5.61-12.84%, 9.65-19.05%, 0.89-7.71%, and 10.11-19.71%, respectively, when orange peel oil is blended with SiO2 and DTBP, and the mixture 10DTBP/90OPO -50SiO2 is found best comparable to ordinary diesel.</p>

Surface modified ceramic membrane for natural volatile oil enrichment with high flux and high component retention

The natural volatile oil has high medicinal value, but the extraction and separation technology were limited by low composition, complex composition, and strong volatility. This study validated the feasibility of membrane separation technology for enriching volatile oil from the aspects of membrane separation efficiency, volatile oil components, and drug efficacy. A shellac coated alumina ceramic (lac@AC) membrane with irregular nanoneedle-like surface structure was prepared to enrich the volatile oil from vinegar-processed Cyperi Rhizoma (vpCR). Compared to the alumina ceramic (AC) membrane, the lac@AC membrane exhibited 29.77 % and 98.05 % increase in water and emulsion flux, respectively. Meanwhile, the oil rejection of lac@AC membrane increased 26.29 %. According to the gas chromatography-mass spectrometry analysis, the similarity between the volatile oils obtained by membrane separation and the traditional steam distillation method was higher than 99.5 %. In addition, antibacterial experiments showed that the volatile oil obtained by membrane separation has similar antibacterial effects to the volatile oil obtained by steam distillation. Component analysis and pharmacodynamic analysis both demonstrated the feasibility of using membrane separation to obtain vpCR volatile oil. This study offers a technical reference for the efficient, high-quality, and environmentally friendly extraction of natural plant oils with complex ingredients.

Patchouli essential oil: Unlocking antimicrobial potential for dandruff control

Abstract Dandruff causes the scalp to peel and results in itching, which is caused by one of the fungi, Malassezia globosa. Several studies have revealed that the compounds in essential oils can act as antimicrobials. To broaden insights regarding the potential compounds in essential oils, this research focuses on the potential of patchouli essential oil in inhibiting the growth of dandruff-causing microbes. One of the compounds with antimicrobial potential in patchouli oil is patchouli alcohol. The steam distillation method was used for extraction oil from dried patchouli stems and leaves to produce three different types of oil samples: crude patchouli oil, heavy fraction patchouli oil, and light fraction patchouli oil. Subsequently, the GC-MS analysis was conducted to compare the compound contents in samples. Our results indicate that the three patchouli oil samples have varying patchouli alcohol contents. Crude patchouli oil contains 31.2% patchouli alcohol; heavy fraction patchouli oil contains 61.82% patchouli alcohol, while light fraction patchouli oil contains 23.99% patchouli alcohol. This study demonstrates that heavy fraction patchouli oil has a greater potential as an antimicrobial compared to crude patchouli oil and light fraction patchouli oil.

Evaluation of the stability of hop pellets subjected to different storage conditions during three years

It is common knowledge that the composition of hops secondary metabolites depends on several factors, among which the storage conditions. In recent years, many studies have been conducted to evaluate changes in the chemical composition of hop products in response to different storage temperatures both in aerobic and anaerobic environments. However, little knowledge exists regarding the storage of hop pellets under modified atmosphere for a very long period of time. The aim of this study is to evaluate the effect on hop quality parameters of different storage conditions on U.S. hop cultivar pellets (Chinook and Nugget) in a three-year trial. Storage in modified atmosphere (atmosphere saturated with 70% nitrogen (N2) and 30% carbon dioxide (CO2) and vacuum storage were tested at two different temperatures, 4 °C and 10 °C. In order to establish the best storage condition, the bitter acids content (HPLC-UV method), the essential oil content (steam distillation method) and the essential oil profile (GC-MS method) were determined and characterised at 0, 6, 12 and 36 months. No statistical differences were observed between samples stored under different atmospheres, while significant differences were highlighted as a result of the storage temperature, e.g. the alpha acids content at 4 °C was significantly higher (P = 0.002). The two hop cultivars showed different behaviours in the storage trial, suggesting that high alpha hop varieties, like Nugget, could be stored even at higher temperatures.

Isolation and characterization of isomeric tibetin spiroethers from the roots of Tanacetum dolichophyllum (Kitam.) Kitam

The roots of Tanacetum dolichophyllum (Kitam.) Kitam. (syn. Hippolytia dolichophylla (Kitam.) K.Bremer & Humphries) were collected from high altitude area of Munsyari, district Pithoragarh (Uttarakhand, India) yielded essential oil by steam distillation method and the oil was analysed by TLC and GC-MS. The GC-MS analysis of the essential oil sample showed the dominance of two constituents visible in sesquiterpene range. These constituents were isolated by column chromatography. The structures of these compounds were determined on the basis of 1H-NMR,13C NMR, COSY, 135-DEPT, and HRMS (ESI-TOF) spectral data. The two major compounds were identified as isomeric C14-Tibetin spiroethers, namely (E)-2-(2’,4’-heptadiyn-1’-ylidene)-1,6-dioxaspiro[4.4]non-3-ene and (Z)-2-(2’,4’-heptadiyn-1’-ylidene)-1,6-dioxaspiro[4.4]non-3-ene.

Comparative chemical profiling of leaf essential oils from Cinnamomum kanehirae and related species using steam distillation and solvent extraction: Implications for plant-based classification

Cinnamomum kanehirae Hayata, belonging to Lauraceae family, is an indigenous and endangered species of considerable economic importance in Taiwan. It plays a crucial role as the host for the economically valuable saprotrophic fungus, Taiwanofungus camphorates. However, accurate species identification poses a challenge due to the similarity in morphological features and frequent natural hybridization with closely related species. Acquiring high-quality and pure leaf oils becomes imperative for precise species identification and producing superior goods. In this study, our objective was to establish methodologies for analyzing the chemical composition of leaf essential oils and subsequently apply this knowledge to differentiate among three Cinnamomum species. Gas chromatography-mass spectrometry (GC/MS) was employed to scrutinize the chemical makeup of leaf essential oils from three closely related species: C. kanehirae, C. micranthum, and C. camphora. We utilized Steam Distillation (SD) and steam distillation-solvent extraction (SDSE) methods, with the SDSE-Hexane approach chosen for optimization, enhancing extraction efficiency and ensuring essential oil purity. Through the SDSE-Hexane method, we identified seventy-four compounds distributed across three major classes: monoterpenes hydrocarbons (0.0–7.0 %), oxygenated monoterpenes (3.8–90.9 %), sesquiterpenes hydrocarbons (0.0–28.3 %), and oxygenated sesquiterpenes (1.6–88.1 %). Our findings indicated the presence of more than one chemotype in both C. kanehirae and C. camphora, whereas no specific chemotype could be discerned in C. micranthum. Furthermore, clustering based on chemotypes allowed for the differentiation of samples from the three species. Notably, we demonstrated that the chemical compositions of grafted C. kanehirae remained largely unaffected by the rootstock. Conversely, natural hybrids between C. kanehirae and C. camphora exhibited profiles more closely aligned with C. kanehirae. The optimized extraction method and the chemotype-based classification system established in this study present valuable tools for essential oil preparation, species identification, and further exploration into the genetic variation of Cinnamomum.

Extraction of Essential Oil from Moringa Oleifera Leaves Using Steam Distillation and Soxhlet Extraction Method

Essential oil is one of Indonesia's agro-industrial export commodities, one of which is essential oil from Moringa oleifera leaves. This study aims to study the results of the yield and analyze the essential oil content of Moringa oleifera leaves using the steam distillation method and Soxhlet extraction using ethanol and isopropyl alcohol as a solvent. The fresh feed was either directly extracted or pre-treated using an oven and then followed by several stages of extraction. The pre-treatment of fresh feed was carried out in the oven at 70°C for 1 hour. Atmospheric steam distillation was carried out at 100°C for 6 hours using 500 ml of distilled water. In the case of soxhlet extraction, the temperature was set at the boiling point of the solvent for 6 hours. Once soxhlet extraction was complete, the essential oil was separated from the solvent by distillation. After various extraction processes, the essential oils were analyzed for their compound content using GC-MS. Based on the results of the study, the highest yield of 29.04% was obtained from the treated feed using two stages of soxhlet extraction variable with ethanol as solvent.

Comparative assessment of anti-ulcer effects: Hydro-ethanolic extract from air-dried ray and disc florets vs essential oil of traditional Tagetes erecta (Asteraceae) in Swiss Albino rats

The Tagetes erecta Linn (family: Asteraceae) herb possesses medicinal and antioxidants value. The herb is traditionally used to treat various ailments, including ulcers. In order to investigate antiulcer activity of T. erecta herb, experimental study was performed between ray and disc florets extracted with 30 parts of water and 70 parts of ethanol solvents using Soxhlet apparatus and leaf's essential oil extracted in Clevenger apparatus by steam distillation method. Two doses of the hydroethanolic extract (400 and 800 mg/kg, Per os) and single dose of essential oil (10 ml/Kg, Per os) were administered for five consecutive days. Rat's pyloric ligation and aspirin (200 mg/kg, Per os) were used to induce acute and chronic gastric ulcers respectively. Antiulcer activity of both the herb's parts was compared on gastric juice parameters (pH, free acidity, total acidity) ulcer-index and ulcer percent protection. The p value of less than 0.05 was considered significant in statistical analysis. Elevation in pH, reduction in free acidity, total acidity and ulcer-index in both the ulcer models confirm that the test drug showed the ulcer-protective activity in rats. Dose-dependent effects were observed in the antiulcer activity of the hydroethanolic extract. However, the leaf's essential oil showed 94 % and 96.93 % ulcer protection in the pyloric ligation and aspirin-induced gastric ulcer models respectively. The hydroethanolic extract and the essential oil may be capable in part, regulating gastric acid secretion pathways or stimulating the cytoprotective secretion of bicarbonate, mucus and prostaglandins. Histological studies supported the observed antiulcer activity of the herb. The essential oil has more potent and significant anti-ulcer activity than hydro-ethanolic extract and respective standard drugs: ranitidine (50 mg/kg, Per os) & pantoprazole (20 mg/kg, Per os). The herb exhibits antiulcer activity, which could be due to the presence of lead phytochemicals: Quercetin-3-methyl ether, quercetin-7-methyl-ether & kaempferol flavonoids in the extract and limonene & terpinolene in leaf's essential oil and, thus, confirmed the traditional uses of T. erecta in the treatment of ulcers. The herb may cure acid pepsin disorders.

Antibacterial Potency and Physicochemical Profiles of Eucalyptus pellita Leaf Waste Essential Oil from PT Surya Hutani Jaya, East Kalimantan

Eucalyptus is one of the fast growing species plants, currently used in the pulp and paper industry. The leaves of Eucalyptus are known as forest harvesting waste. This research aim to examine the antibacterial potency and the physicochemical profiles of Eucalyptus pellita leaves waste essential oils grown in PT Surya Hutani Jaya Site 32, East Kalimantan named EP B077. The essential oils distilled using water and steam distillation methods. Analysis of physicochemical profiles from this oil included yield, colour, refractive index, specific gravity, and chemical compositions by GC-MS. Antibacterial activity assayed by agar diffusion method against Staphylococcus epidermidis, Streptoccoccus sobrinus and Streptoccoccus mutans with slight modification. The results showed that EP B077 had the yellow colour, yield oils was 0.44%, 1.471 of refractive index, and 0.8706 of specific gravity. Chemical components of EP B077 oils were dominated by α-Pinene (40.36%), 1-β-Pinene (31.75%), Cyclohexene, 1-methyl-4-(1-methylethenyl)- (9.64%), Trans(β)-Caryophyllene (4.29%) and Eucalyptol (1,8-Cineole) (3.88%). This EP B077 oils was active to inhibit bacteria against S. epidermidis and S. sobrinus range from 9-15 mm, also against S.mutans ranging from 9-14 mm. Based on the results, this Eucalyptus pellita leaves named EP B077 had potential to develop as a new source of essential oil and antibacterial agents.

Chemical composition and antibacterial activity of essential oil kaffir lime (Citrus hystrix DC) leaves from East Borneo

Kaffir lime (Citrus hystrix DC) leaves are a versatile plant with high commercialization potential. In the pharmaceutical industry, kaffir lime leaf essential oil is used to stimulate appetite, as a cosmetic ingredient, and as an antibacterial agent. Although most studies have concentrated on the essential oil of kaffir lime leaves, its components and biological activities need to be considered based on the growing location. The chemical components and biological activity of kaffir lime leaf essential oil are affected by differences in growth location. This research was conducted to determine the chemical composition and antibacterial activity of the essential oil of kaffir lime leaf essential oil from East Borneo. Fresh and dried kaffir lime leaves were extracted by steam distillation method and chemical compound analysis by Gas Chromatography-Mass Spectrometry (GC-MS) and the antibacterial activity of essential oils was analyzed by disc diffusion method. There are twenty-five compounds in the essential oil of fresh and dried kaffir lime leaves. The main components of fresh kaffir lime leave essential oil were citronellal (77.29%), linalool (4.88%), sabinene (4.83%), citronellol (3.41%), β-myrcene (1.23%), and citronellal (74.09%), sabinene (5.54%), linalool (3.59%), citronellol (3.56%), caryophyllene (2.26%) were the main components of the essential oil of dried kaffir lime leaves. The antibacterial activity of essential oils against Staphylococcus aureus ATCC 25925 and Escherichia coli ATCC 8733, with zones of inhibition of 21.75 and 19.17 mm, respectively. The minimum inhibitory concentration on all antibacterial activities was 6.25 μL/mL. The presence of strong antibacterial activity in dried and fresh kaffir lime leaf plant extracts was associated with the presence of citronellal compounds found in essential oil kaffir lime leaves