Lipid Peroxidation Status Research Articles

Evaluation of Antioxidant, Lipid Peroxidation and Toxic Effects after Pomegranate Intake in Healthy Human Volunteers

Studies have extensively demonstrated the activation of enzymic and nonenzymic antioxidants as well as decrease of lipid peroxidation status after pomegranate intake without any observable toxicity in animal models. Comparing to animal model studies, human trials are less done on these aspects. Cellular damage caused by reactive oxygen species appears to be a major contributor in ageing and other degenerative diseases such as cancer, cardiovascular diseases, cataracts, compromised immune system, rheumatoid arthritis and brain dysfunction. Several literatures show that pomegranate has been used as a folk medicine from ancient times itself. As per the reviews, pomegranate fruit possesses antioxidant, anti-inflammatory, antiatherogenic and antitoxic effects. The primary objective of this study was to evaluate the circulatory levels of selected antioxidant and lipid peroxidation marker in healthy human volunteers before and after pomegranate intake for a period of 0 - 90 days. As the secondary objective of the study, we also assessed the toxic effects of pomegranate supplementation by determining Hepatobiliary and Renal function tests. The final result showed that majority of the study population showed a significant increase in enzymic and non-enzymic antioxidants and a decrease in oxidative stress after pomegranate intake without any observable toxicity in liver function and renal function. The significant healthy variations were more after the intake of pomegranate for a period of 90 days than that of 45 days.

Effects of dietary supplementation with carnosine on meat quality and antioxidant capacity in broiler chickens

ABSTRACT1. This study aimed to investigate the effects of carnosine supplementation on meat quality, antioxidant capacity and lipid peroxidation status in broiler chickens.2. A total of 256 1-d-old male Arbor Acres broilers were randomly assigned to 4 treatments consisting of 8 replicates of 8 chickens each. The birds were supplied with 4 different diets: a basal diet or a basal diet supplemented with 100, 200 or 400 mg/kg carnosine, respectively. The whole experiment lasted 42 d.3. The results showed that dietary supplementation with carnosine linearly increased the values of pH45 min and redness and reduced drip loss of breast meat. Dietary carnosine increased the activity of antioxidant enzymes in liver, serum and breast meat and decreased the contents of lipid peroxides at 21 and 42 d of age.4. These findings indicated that dietary supplementation with carnosine was beneficial to enhance meat quality, antioxidant capacity and decrease lipid peroxidation status of breast meat.

Effects of silver nanoparticles of different sizes on cytotoxicity and oxygen metabolism disorders in both reproductive and respiratory system cells

Abstract Silver nanoparticles (AgNPs) are widely used in numerous industries and areas of daily life, mainly as antimicrobial agents. The particles size is very important, but still not suffi ciently recognized parameter infl uencing the toxicity of nanosilver. The aim of this study was to investigate the cytotoxic effects of AgNPs with different particle size (~ 10, 40 and 100 nm). The study was conducted on both reproductive and pulmonary cells (CHO-9, 15P-1 and RAW264.7). We tested the effects of AgNPs on cell viability, cell membrane integrity, mitochondrial metabolic activity, lipid peroxidation, total oxidative and antioxidative status of cells and oxidative DNA damage. All kinds of AgNPs showed strong cytotoxic activity at low concentrations (2÷13 μg/ml), and caused an overproduction of reactive oxygen species (ROS) at concentrations lower than cytotoxic ones. The ROS being formed in the cells induced oxidative damage of DNA in alkaline comet assay. The most toxic was AgNPs<10 nm. The results indicate that the silver nanoparticles, especially less than 10 nm, may be harmful to the organisms. Therefore, risk should be considered when using nanosilver preparations and provide appropriate protective measures when they are applied.

Molybdenum Stress Modulates Enzymes Responsive to Oxidative Stress and Affects Seeds Viability and Vigor in Chickpea

ABSTRACTThe importance of molybdenum (Mo) for plant growth is disproportionate with respect to the absolute amounts required by most plants. Chickpea (Cicer arietinum L.) cv. K-75 plants were raised in refined sand in glasshouse at graded levels from 0.002 to 1 µM for 100 days. Mo deficiency symptoms appeared as interveinal chlorosis of middle and old leaves. Compared with the control (0.2 µM Mo), dry matter, yield and seed protein decreased at low and excess Mo. The concentration of Mo in leaves and seed as well as the activity of nitrate reductase (NR) increased with an increase in Mo supply. The activities of antioxidative enzymes stimulate at both low and excess Mo supply. Low and excess Mo decreased the lipid peroxidation status in chickpea leaves, suggesting its antiperoxidative nature. The values of deficiency, threshold of deficiency and threshold of toxicity of Mo were, respectively, 0.38, 1.2 and 15 µg g−1 in leaves of chickpea.

In silico analysis of candidate proteins sharing homology with Streptococcus agalactiae proteins and their role in male infertility

ABSTRACTLeukocytospermia is a physiologic condition defined as human semen with a leukocyte count of >1 x 106 cells/ml that is often correlated with male infertility. Moreover, bacteriospermia has been associated with leukocytospermia ultimately leading to male infertility. We have found that semen samples with >1 x 106/ml leukocytes and/or bacteriospermia have oxidative predominance as evidenced by augmented protein carbonyl and lipid peroxidation status of the semen which is implicated in sperm dysfunction. It has been reported that Streptococcus agalactiae is present in bacteriospermic samples. Previous research has shown that human leukocyte antigen beta chain paralog (HLA-DRB) alleles interact best with the infected sperm cells rather than the non-infected cells. Little is known about the interaction of major histocompatibility complex (MHC) present on leukocytes with the sperm upon bacterial infection and how it induces an immunological response which we have addressed by epitope mapping. Therefore, we examined MHC class II derived bacterial peptides which might have human sperm-related functional aspects. Twenty-two S. agalactiae proteins were obtained from PUBMED protein database for our study. Protein sequences with more than two accession numbers were aligned using CLUSTAL Omega to check their conservation pattern. Each protein sequence was then analyzed for T-cell epitope prediction against HLA-DRB alleles using the immune epitope database (IEDB) analysis tool. Out of a plethora of peptides obtained from this analysis, peptides corresponding to proteins of interest such as DNA binding response regulator, hyaluronate lyase and laminin binding protein were screened against the human proteome using Blastp. Interestingly, we have found bacterial peptides sharing homology with human peptides deciphering some of the important sperm functions. Antibodies raised against these probable bacterial antigens of fertility will not only help us understand the mechanism of leukocytospermia/bacteriospermia induced male factor infertility but also open new avenues for immunocontraception.Abbreviations: AA: amino acid; ASA: antisperm antibodies; GBS: group B streptococcus; HLA: human leukocyte antigen; HAS3: hyaluronan synthase 3: IEDB: immune epitope database; MAPO2: O6–methylguanine–induced apoptosis 2; MHC: major histocompatibility complex; ROS: reactive oxygen species; Rosbin1: round spermatid basic protein 1; S. agalactiae: Streptococcus agalactiae;SA: sperm antigen; SPATA17: spermatogenesis associated protein17; SPNR: spermatid perinuclear RNA binding protein; TEX15: testis-expressed sequence 15 protein; TOPAZ: testis– and ovary-specific PAZ domain–containing protein; TPABP: testis-specific poly–A binding protein; TPAP: testis–specific poly(A) polymerase; WHO: World Health Organization

Status of photosynthetic pigments, lipid peroxidation and anti-oxidative enzymes in Vigna mungo in presence of arsenic

ABSTRACTThe response to arsenic was evaluated for photosynthetic pigments, lipid peroxidation and anti-oxidative enzymes in black gram. Black gram (Vigna mungo) subjected to arsenic doses of 100 µM and 200 µM, showed increased amount of lipid peroxidation. Activity of peroxidase (POD) increased tremendously in arsenic treated Vigna leaves over control. Similarly, activities of enzymes like superoxide dismutase (SOD) and ascorbate peroxidase (APX) also increased with increase in arsenic. Analysis of native PAGE superoxide dismutase activity showed one manganese (Mn)-SOD and two copper/zinc (Cu/Zn)-SOD isoenzymes in black gram leaves, whose intensity increased especially at 200 µM arsenic treatment. However, activity of catalase (CAT) decreased with increase in concentrations of arsenic. This clearly indicates that except CAT, all studied antioxidative enzymes like SOD, POD and APX showed increased expression at arsenic treatments, which reflects their protective role against arsenic toxicity in black gram plants. Photosynthetic pigments like chlorophyll and carotenoids showed reduction with increasing concentrations of arsenic in treatment solutions.

Lipid peroxidation and total antioxidant status in rural communities exposed to fluoride and arsenic in Chihuahua, Mexico

Lipid peroxidation and total antioxidant status in rural communities exposed to fluoride and arsenic in Chihuahua, Mexico

The effect of oxidative stress during exercise in the horse.

Oxidative stress is an imbalance of the oxidant-to-antioxidant ratio in the body. Increases in oxidative stress and changes in antioxidant status have been shown during endurance and intense exercise and eventing competition in horses. Antioxidants include vitamins, minerals, enzymes, and proteins that must be synthesized in the body or obtained from the diet. Therefore, exercise level and diet are both factors that play a role in influencing the oxidative stress and antioxidant status of the equine athlete. Along with exercise intensity and duration, diet, age, and training program can also affect oxidative stress in the horse. Several studies using exogenous supplementation of vitamin E, vitamin C, and alpha-lipoic acid have shown positive results in decreasing the effects of exercise (endurance and intense exercise)-induced oxidative stress and increasing the antioxidant status based on the markers and antioxidants measured, whereas other studies using superoxide dismutase showed little effects on the exercise horse. The "free radical theory of aging" states that long-term effects of the degenerative changes associated with aging may induce oxidative stress. However, in old horses (22 ± 2 yr), lipid peroxidation levels and blood antioxidant concentrations were similar to those found in younger but mature (12 ± 2 yr) horses both at rest and during exercise. Other studies found that yearlings (18 ± 2.4 mo) that are novel to forced exercise had less lipid peroxidation and greater antioxidant status than mature mares (13 ± 2.1 yr) prior to exercise training. Exercise training reduced oxidative stress markers and improved antioxidant status in mares, whereas few effects were seen in yearlings. This indicates that youth provided more defense against oxidative stress due to exercise than the exercise training program. Other studies during competition (endurance, jumping, eventing, and racing) have investigated the influence on oxidative stress with varying results. Despite the multitude of studies examining the levels of lipid peroxidation, antioxidant status, and other related metabolites in the horse during exercise, we still have a long way to go before we fully understand the large variation in results both with and without antioxidant supplementation.

Total antioxidant status and lipid peroxidation with and without in vitro zinc supplementation in infertile men.

The aim of this study was to assess the total antioxidant capacity (TAC) and malondialdehyde (MDA) level in infertile men with asthenozoospermia and asthenoteratozoospermia compared to fertile donors, and to examine the effect of zinc on sperm lipid peroxidation and antioxidant status in infertile and fertile men. Semen samples provided by infertile men (n=38) and fertile donors (controls; n=12) were exposed to 6mmol/L of zinc for 2hr at 37°C. After semen analysis, lipid peroxidation was detected by MDA assay and seminal TAC was assessed by colorimetric method using TAS (total antioxidant status) Kit. TAC was significantly lower in infertile group compared to controls (p=.037). However, lipid peroxidation did not alter in infertile patients compared to controls (p>.05). After in vitro incubation of samples with zinc, a significant increase in TAC level was found only in infertile men (p<.001). Meanwhile, zinc had no effect on sperm lipid peroxidation in both fertile and infertile men (p>.05). Our data indicate that antioxidant treatment based on zinc in vitro supplementation may be helpful to enhance the rate of seminal antioxidant status in infertile men; however, it does not prevent sperm lipid peroxidation.

Effect of different temperature humidity indices onantioxidant parameters in Surti buffaloes

The present study was conducted on 20 Surti buffalo heifers of 16 to 21 months age maintained under standard feeding and management practices. They were categorized based on their exposure to natural environment as Group I (Hot dry season: THI1), Group II- (Hot humid season: THI2) and Group III as Control group (Comfortable season: THI3). Whole blood and serum samples were collected from all the groups and analyzed for antioxidant parameters, viz., superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase, lipid peroxidation (LPO) and total antioxidant status (TAS). The temperature humidity index (THI) calculated for Group I, Group II and Group III were 81.70 (THI1), 80.30 (THI2) and 70.00 (THI3). For Group I, Group II and Group III, concentration of SOD (U) was 5.29±0.06, 4.65±0.04 and 2.94±0.09; GPx (U/ml) was 43.63±0.71, 36.40±0.56 and 25.84±1.00; catalase (µmoles/min/ml) was 1,609.17±45.93, 1,322.00±18.65 and 1,106.35±45.07; LPO (nM of MDA/ml of packed cells) was 6.00±0.13, 3.45±0.09 and 2.87±0.14 and TAS (mmol/l) was 5.18±0.11, 2.90±0.09 and 2.37±0.13, respectively. Significantly (P is less than 0.01) higher levels of SOD, GPx, catalase, TAS and LPO were observed in hot dry season (THI1) as compared to hot humid (THI2) and comfortable (THI3) seasons. Increased levels of SOD, GPx, catalase, TAS and LPO indicated that hot dry season is more stressful for animals as compared to hot humid season in Surti buffaloes as they are inhabitants of hot humid region

ПОКАЗНИКИ ПЕРОКСИДНОГО ОКИСНЕННЯ ЛІПІДІВ У КРОВІ ВІВЦЕМАТОК, ХВОРИХ НА ГЕПАТОДИСТРОФІЮ

The article deals with the results of studies on the causes and clinical manifestations of hepatodystrophy in ewes, indicators status of lipid peroxidation – TBA-active products, lipid hydroperoxides, diene conjugates.In order to establish the causes of hepatodystrophy in sheep, we have analyzed the feed rations. Sheep investigated 24 local breeds aged 3 – 4 years, weighing 45 – 56 kg. Conducted clinical study animals and blood were collected for laboratory analysis. Serum sheep determined the content of malondialdehyde (MDA-active products) using thiobarbituric acid (Stalnaja I.D., Haryshvyly T.G, 1977) and lipid hydroperoxides using ammonium thiocyanate (Romanova LA, Stalnaja I.D., 1977). In blood examined diene conjugates (Stalnaja I.D., 1977).High performance is ensured animals by creating appropriate technical conditions and feeding. Violation of maintenance processes and non-biological nutrition of the cause performance degradation, the spread of disease and culling of animals. Development hepatodystrophy animals observed in animals fed low-quality forage and depraved that contain a number of toxic products of oxidation of fats – aldehydes, ketones, low molecular weight acids, hydroxy acids, which have toxic properties and also due to an unbalanced diet for the main nutrients and biologically active substancesThe main causes of primary hepatodystrophy in sheep is imbalanced nutrition and lack of essential nutrients in the diet. In analyzing the diet of established lack of dry matter, raw protein, digestible protein, raw fiber, sugar and starch. Value sugar and starch with digestible protein accounted for 1,78: 1, and sugar-protein ratio – 0,77. Rations were scarce for vitamins D and E, microelements – Sulphur, copper, zinc, cobalt and iodine.In ewes suffering from hepatodystrophy was set the development of oxidative stress – increase in blood activity of TBA-active products, lipid hydroperoxides, diene conjugates. In particular, concentration of diene conjugates was increased by twice the number of hydroperoxides lipids was increased by three times and the activity of TBA-active products was increased by five times compared with clinically healthy animals. Histological examination of liver biopsies of ewes was set that the develop of protein granular and fatty degeneration of hepatocytes.

Oxidative Stress and Antioxidant Status in Standardbreds: Effect of Age and Training in Resting Plasma and Muscle

The hypothesis of this study was that yearlings would have more oxidative stress as measured by malondialdehyde (MDA), total glutathione (GSH-T), glutathione peroxidase (GPx), and nitric oxide (NO) than mature mares before training, but after they would have similar levels. Ten Standardbred yearling fillies and 10 mature Standardbred mares were split into trained and nontrained groups. Horses were trained 5 days/wk for 7 weeks. Blood and muscle samples were collected at rest on weeks 0, 2, 5, 7, and 9. At week 0, the yearlings had higher muscle GSH-T and NO concentrations (P = .03) than the trained mares. At week 9, plasma NO concentrations were lower in trained mares than in the trained yearlings (P = .007). Trained mares increased muscle MDA and decreased plasma MDA concentrations from weeks 0 to 9 (P < .01), while all mares increased muscle NO and GSH-T concentrations by week 9 (P < .05). Trained mares and yearlings had increased erythrocyte GPx activity at weeks 7 and 9 and GSH-T concentration at week 7 (P < .05). Mares had higher lipid peroxidation and lower antioxidant status in the muscle than the yearlings prior to training. Trained mares improved antioxidant status and oxidative stress levels through training, resulting in levels similar to the yearlings.

Identification of suitable combinations of in vitro sperm-function test for the prediction of fertility in buffalo bull

The present study assessed sperm functional characteristics in the frozen-thawed semen of buffalo bulls and estimated their relationship with field fertility. Frozen semen samples from three different freezing operations each from nine Murrah buffalo bulls were used for the assessment of different sperm functions related to fertilizing potential. Bulls were classified into high (n = 2), medium (n = 5), and low (n = 2) fertile based on adjusted field fertility. The sperm functions estimated included membrane integrity using carboxyfluorescein diacetate-propidium iodide, acrosome reaction status using fluorescein isothiocyanate peanut agglutinine, status of apoptosis using Annexin-V, protamine deficiency using Chromomycin A3, membrane stability using Merocyanine 540 and lipid peroxidation status using 4, 4-difluoro-4-bora-3a, 4a-diaza-s-indacene. The relationship between the proportion of live acrosome-intact spermatozoa and fertility was positive and significant (r = 0.59; P = 0.001). The proportion of moribund spermatozoa showed a significantly negative correlation with fertility (r = −0.50; P = 0.008). Similarly, the relationship of spermatozoa with unstable membrane (r = −0.51; P = 0.007), necrotic (r = − 0.42; P = 0.028), early necrotic (r = −0.42; P = 0.031), and apoptotic spermatozoa (r = −0.39; P = 0.046) with bull fertility was negative and significant. The correlation between the protamine-deficient spermatozoa and fertility was negative, but not significant. Among different combinations of tests, live acrosome-intact spermatozoa and lipid peroxidation status of spermatozoa revealed high positive correlation with buffalo bull fertility (adjusted R2 = 0.73, C[p] = 0.80). These preliminary findings may help in developing tools for assessing fertility of buffalo bulls, once validated in more animals.

Comparative analysis of in vitro characteristics of fresh and frozen-thawed urethral and epididymal spermatozoa from cats (Felis domesticus)

The first aim of this study was to provide a comprehensive analysis of structural and functional features of spermatozoa in semen collected from the same cat by two methods: urethral catheterization and epididymis slicing. The second aim was to assess if feline urethral (CT) and epididymal (EP) spermatozoa undergo the same changes during cryopreservation and to compare the postthaw characteristics of spermatozoa collected by the two methods. In the first phase, CT and EP semen were collected from 20 cats, and sperm motility, viability, morphology, computer-assisted sperm analysis (CASA) parameters, membrane and acrosome integrity, mitochondrial potential, lipid peroxidation, and chromatin status were assessed. In the second phase, both types of semen collected from 10 cats were cryopreserved, thawed, and the same sperm parameters were assessed as in fresh semen. Fresh CT spermatozoa (phase I) showed higher (P < 0.05) motility (subjective: median 75.0% vs. 62.5%; by CASA: mean ± SD 60.2 ± 10.7% vs. 43.1 ± 16.7%), morphology (mean ± SD, 57.5 ± 9.6% vs. 45.2 ± 15.9%), and membrane integrity (median live: 89.2% vs. 79.8%). Other parameters were not different between CT and EP spermatozoa. After cryopreservation (phase II), spermatozoa from both types of semen did not differ significantly, except for lipid peroxidation of live sperm cells (median CT: 3.5%, EP: 1.7%, P < 0.05). Urethral and EP spermatozoa showed a similar, significant drop in motility (CT to 18.6 ± 10.3% and EP to 21.6 ± 12.1%, P < 0.05), progressive motility (CT to 6.8 ± 5.9% and EP to 8.3 ± 8.8%, P < 0.05), and rapid movement (from 34.3 ± 20.6% to 8.5 ± 7.0% in CT and from 26.0 ± 14.7% to 10.1 ± 10.4% in EP, P < 0.05), whereas other motion characteristics assessed by CASA were not affected (P > 0.05). The strongest change after cryopreservation was noted in high mitochondrial potential (median CT: 1.3%, EP: 2.2%). Although cryopreservation increased acrosome damage and lipid peroxidation, the level of these changes in the population of live sperm cells remained low (median acrosome damage: CT: 3.3%, EP: 4.5%, lipid peroxidation CT: 3.5%, EP: 1.7%). Cryopreservation did not affect chromatin structure (median percent DNA fragmentation index, CT: 3.3%, EP: 2.3%). In this study, we confirmed that urethral catheterization for collection of semen allows the retrieval of spermatozoa with quality equally good as in those obtained by epididymal slicing. Spermatozoa from both types of semen collected showed similar characteristics after freezing/thawing so both types can be used for semen banking.

A relevant exposure to a food matrix contaminated environmentally by polychlorinated biphenyls induces liver and brain disruption in rats

Polychlorinated biphenyls (PCBs) are ubiquitous environmental contaminants present in dietary fats. Most studies evaluating PCB effects have been conducted with a single compound or a mixture of PCBs given as a single acute dose.The purpose of this study was to evaluate in vivo PCB toxicity in a realistic model of exposure: a low daily dose of PCBs (twice the tolerable daily intake (TDI)), chronically administered (8 weeks) to rats in contaminated goat milk. Liver and brain PCB toxicities were investigated by evaluating oxidative stress status and mitochondrial function. PCB toxicity in the liver was also estimated by transaminase enzymatic activity.This study shows that even at low doses, chronic PCB exposure resulted in a statistically significant reduction of mitochondrial function in liver and brain. In the liver, oxygen consumption in the condition of adenosine triphosphate (ATP) production (state 3) decreased by 22–29% (p < 0.01), according to the respiratory substrates. In the brain, respiratory chain complexes II and III were reduced by 24% and 39%, respectively (p < 0.005). The exposed rats presented higher lipid peroxidation status (+20%, p < 0.05) and transaminase activity (+30%, p < 0.05) in the blood.Thus, our study showed that exposure of rats to a daily realistic dose of PCBs (twice the TDI in a food complex mixture of environmental origin) resulted in multiple disruptions in the liver and brain.

Modulating effects of vanillic acid on circadian pattern of indices of redox homeostasis in N-Methly-N′-Nitro-N-Nitrosoguanidine induced endometrial carcinoma in rats

The circadian timing system controls drug metabolism and cellular proliferation over the 24-h period through molecular clocks in every cell. Accumulating epidemiological and genetic evidences indicate that the disruption of circadian rhythms might be directly linked to cancer. This study evaluates the effect of vanillic acid on the circadian rhythms of circulatory lipid peroxidation and antioxidant status during N-Methyl-N′-Nitro-N-Nitrosoguanidine (MNNG)-induced endometrial carcinoma in rats. The characteristics of circadian rhythms (acrophase, amplitude and mesor) of lipid peroxidation products – thiobarbituric acid reactive substances (TBARS), lipid hydroperoxides (LOOH) and enzymatic antioxidants like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and non-enzymatic antioxidants such as reduced glutathione (GSH), vitamins C and E were markedly declined in MNNG-treated rats when compared to other groups. Pre and co-treatment of vanillic acid to MNNG-treated animals significantly increased the mesor and altered amplitudes of antioxidants and significantly decreased the mesor values of TBARS and LOOH. Further, delays in acrophase in MNNG-induced rats were reversed by vanllic acid administration. Thus, oral treatment of vanillic acid results in normalization of the altered rhythms of these indices of redox homeostasis (compared to controls) by its anticarcinogenic, cytoprotective and antioxidant effects.

Oleuropein attenuates cognitive dysfunction and oxidative stress induced by some anesthetic drugs in the hippocampal area of rats

The present study was designed to evaluate the antioxidant effects of oleuropein against oxidative stress in the hippocampal area of rats. We used seven experimental groups as follows: Control, Propofol, Propofol-Ketamine (Pro.-Ket.), Xylazine-Ketamine (Xyl.-Ket.), and three oleuropein-pretreated groups (Ole.-Pro., Ole.-Pro.-Ket. and Ole.-Xyl.-Ket.). The oleuropein-pretreated groups received oleuropein (15mg/kg body weight as orally) for 10 consecutive days. Propofol 100mg/kg, xylazine 3mg/kg, and ketamine 75mg/kg once as ip was used on the 11th day of treatment. Spatial memory impairment and antioxidant status of hippocampus were measured via Morris water maze, lipid peroxidation marker, and antioxidant enzyme activities. Spatial memory impairment and lipid peroxidation significantly increased in Xyl.-Ket.-treated rats in comparison to the control, propofol, Ole.-Pro. and Ole.-Pro.-Ket. groups. Oleuropein pretreatment significantly reversed spatial memory impairment and lipid peroxidation in the Ole.-Xyl.-Ket. group as compared to the Xyl.-Ket.-treated rats. There was no significant difference between the control and the propofol group in lipid peroxidation and spatial memory status. Superoxide dismutase and catalase activities both significantly decreased in Xyl.-Ket.-treated rats when compared to the control, propofol, Ole.-Pro., Ole.-Pro.-Ket., and Ole.-Xyl.-Ket. groups. In contrast, glutathione peroxidase activity in Xyl.-Ket.-treated rats significantly increased as compared to the control, propofol, Pro.-Ket., Ole.-Pro., and Ole.-Pro.-Ket. groups. We concluded that xylazine in combination with ketamine is an oxidative anesthetic drug and oleuropein pretreatment attenuates cognitive dysfunction and oxidative stress induced by anesthesia in the hippocampal area of rats. We also confirmed the antioxidant properties of propofol as a promising antioxidant anesthetic agent.

Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa

The aim of this study was to evaluate different antioxidants-supplemented freeze-dried egg yolk based extenders for the post-thawing quality and incubation resilience of goat spermatozoa. Pooled semen were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in control and antoxidant supplemented freeze-dried extenders (methionine, cysteamine and butylated hydroxytoluene). Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypoosmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). Membrane lipid peroxidation status was also analyzed using the malondialdehyde (MDA) concentration. In the study, antioxidant supplemented freeze-dried egg yolk based extenders have beneficial effect on goat sperm parameters. In addition, we achieved a higher quality in post thawed goat semen even after 6 h incubation when the extender was supplemented by 5 mM BHT or cysteamine.

Nephroprotective effect of β-sitosterol on N-diethylnitrosamine initiated and ferric nitrilotriacetate promoted acute nephrotoxicity in Wistar rats.

The most abundant plant sterol β-sitosterol is widely used for treating heart diseases and chronic inflammatory conditions. The objective of the current study was to evaluate the nephroprotective effect of β-sitosterol against nephrotoxicants which were studied using renal function markers, antioxidant and lipid peroxidation status, and inflammatory markers. Male albino Wistar rats were randomly grouped into four: group 1 was vehicle control rats (0.1% carboxymethyl cellulose [CMC]); group 2 was rats treated with N-diethylnitrosamine (DEN) (200 mg/kg body weight [bw] i.p. on the 15th day) and ferric nitrilotriacetate (Fe-NTA) (9 mg/kg bw i.p. on 30th and 32nd days); group 3 was rats that received β-sitosterol (20 mg/kg bw in 0.1% CMC, p.o. for 32 days) 2 weeks prior to the exposure to the nephrotoxicant; and group 4 was rats that received β-sitosterol alone. The experiment was terminated after the 24 h of last dosage of Fe-NTA, and all the animals were sacrificed. The blood, liver and kidney from each group were analyzed for biochemical, molecular and histological changes. All the parameters showed significant changes in DEN and Fe-NTA treated animals, whereas β-sitosterol pretreated animals' altered biochemical parameters were restored to near normal. Histopathological and immunoexpression studies on tissues also corroborate the biochemical endpoints. Administration of β-sitosterol to nephrotoxicity induced rats showed significant positive changes in biochemical parameters, histopathological and immunohistochemical observations, and up-regulation of Nrf2 gene expression. From this, it was clear that β-sitosterol showed renal protective function.

Amelioration of age-dependent increase in oxidative stress markers in male mice by extract of Potentilla fulgens

Objective: To investigate the effect of Potentilla fulgens extract on lipid peroxidation and antioxidant status in male mice as a function of age.Methods: Eighteen-month-old Swiss albino male mice were administered the dichloromethane-methanol extract of P. fulgens (250 mg/kg b.w.) on alternate days via intraperitoneal route for a period of 14 days. Lipid peroxidation and activities of catalase (CAT) and glutathione peroxidase (GPx1) in liver and kidney were measured and serum oxygen radical absorbance capacity (ORAC) assay was estimated. Phytochemical analysis of P. fulgens extract using high performance thin layer chromatography (HPTLC) was carried out with gallic acid, quercetin, catechin, and epicatechin as markers.Results: Significant increase in level of thiobarbituric acid-reactive substances (TBARS), decreased GPx1, and CAT activities as well as reduction in ORAC were observed in 18-month-old mice as compared to that of 2-month-old mice. Treatment with P. fulgens extract significantly lowered TBARS level, ameliorated CAT, and GPx1 activities in liver and kidney and improved serum ORAC in aging mice. HPTLC studies revealed well resolved bands of P. fulgens extract containing epicatechin and catechin.Discussion: This study showed that P. fulgens is a potent antioxidative agent, which can emerge as a promising candidate in alleviating the age-associated oxidative stress and related diseases.