Uninterrupted OAC is the peri-procedural antithrombotic strategy of choice in patients undergoing AF ablation to minimize the incidence of peri-ablation thromboembolic event. With NOACs, a minimally-interrupted strategy (i.e., holding 1 dose) is often employed, but the effects on the coagulation status of patients is unknown. To compare clot kinetics in patients under uninterrupted vs minimally-interrupted oral anticoagulation strategy. We compared the thromboelastographic (TEG) profile of 91 consecutive patients who underwent EP procedures on uninterrupted (n=59) or minimally-interrupted (n=32) factor Xa inhibitors (FXaI). Among other parameters, TEG allows to determine the speed of thrombin generation (reaction time, R), a key step of the enzymatic phase of thrombus formation (figure). See figure. Compared to those with uninterrupted FXaI, patients on a minimally interrupted strategy had shorter R times (6.4±0.9 min vs 7.1±1.5 min; mean Δ -0.7 min, 95% CI -0.1 to -1.2 min). When considering patients on uninterrupted apixaban (n=28), the difference in time between apixaban morning dose and blood testing predicted R times, each hour associated with a decrease in R of approximately 0.2 minutes (β = -0.2; p=0.0074). The rate of thrombin generation is slower in patients under uninterrupted FXaI compared to those following a minimally-interrupted strategy. For those on uninterrupted apixaban, the amount of time passed since the morning dose affects clot kinetics, with possible implications for peri-procedural thromboembolic events.