ABSTRACT Barn Owls (Tyto alba) are a species of conservation interest, but management of nesting populations can require significant effort. To focus management activities, we conducted analyses of statewide survey data collected for Barn Owl nests (n = 515) and roosts (n = 145) in Kentucky, USA, from 2010–2022. Nest and roost sites included natural (e.g., trees, rock shelters) and human-made (e.g., barns, attics, hunting blinds, silos, etc.) structures and nest boxes (n = 270) installed on various substrates. Using data related to land cover, agricultural features, and other habitat-related variables, we examined factors influencing Barn Owl site occupancy by creating habitat suitability models. Additionally, we assessed changes in nest box occupancy over time and the factors that influenced nest box occupancy. The Shannon habitat diversity index and number of buildings within 75 m were the most important variables for habitat suitability throughout the study area, though the proportion of hay/pasture/grassland within 625 m and distance to an area intensively managed for Barn Owls were important regionally. Barn Owl nest box occupancy increased over the course of our study, likely due to management efforts. Owls were more likely to occupy nest boxes if they were installed on tall feed silos, retired utility poles, or barns. Moreover, nest boxes installed at sites where we observed owl pairs (prior to installation) were more likely to become occupied and used sooner than nest boxes installed opportunistically. Future management should focus on installing nest boxes for known pairs, on unused feed silos, on retired utility poles on reclaimed surface mines, and near areas intensively managed for the species.