Cauliflower (Brassica oleracea var. botrytis) is an important vegetable in India, where it is mostly grown in the states of Bihar, Gujarat, Haryana, Madhya Pradesh and West Bengal. India is the world's second largest producer of cauliflower with over 8.84 million tonnes produced annually (Food and Agriculture Organization, 2020). In August 2019, sunken, dark brown stem lesions, covered with acervuli, were observed on cauliflower seedlings in commercial fields in Jalna, Maharashtra, India (Figure 1). Approximately 5% of the seedlings were affected by the disease. Five diseased cauliflower seedlings were collected, and surface sterilised with 1% NaOCl for two minutes followed by two washings with sterile distilled water. Surface-sterilised sunken necrotic lesions on the stems were examined under the microscope. Conidial suspensions were obtained by dispensing 20 µl of distilled water onto acervuli. Serial dilutions of the suspensions were plated onto potato dextrose agar (PDA) with 50 mg/l streptomycin sulphate to obtain pure cultures. The plates were then incubated at 28°C with a 16 h light/8 h dark cycle for seven days. The fungal colonies were grey with scattered black conidial masses (Figure 2). The mycelial growth rate on PDA was 10 mm per day. Conidia were cylindrical, thin walled, hyaline and aseptate. The conidia measured 21.1 ± 1.0 × 8.3 ± 0.4 µm (Figure 3). These morphological characters align with the description of the Colletotrichum gigasporum species complex (Liu et al., 2014). For molecular characterisation, the glyceraldehyde 3-phosphate dehydrogenase (GAPDH), parts of the β-tubulin and actin (ACT) genes were amplified (Liu et al., 2014) and sequenced. BLAST analysis in the NCBI database showed 100% identity to GAPDH, β-tubulin and ACT gene sequences of C. gigasporum (GenBank Accession Nos. KF687829, KF687872 and KF687787, respectively). The sequences were deposited in GenBank (MW307265-MW307267) and aligned with published sequences using MEGA11 (Tamura et al., 2021), followed by phylogenetic analysis using the UPGMA method (Figure 4). Phylogenetic analyses confirmed the identification as C. gigasporum. To confirm the pathogenicity of the isolates, leaves of five potted 40-day-old cauliflower seedlings were surface sterilised with 70% ethanol for 30 seconds and inoculated with 10 µl of conidial suspension (c. 105 conidia/ml) obtained from a seven-day-old culture grown on PDA. Leaves of an additional five seedlings were inoculated with sterile water as a control. Typical anthracnose symptoms developed on C. gigasporum-inoculated cauliflower seedlings seven days after inoculation (Figure 5) and no symptoms were seen on control plants. The pathogenicity experiment was repeated twice with similar results. Conidia were re-isolated from symptomatic cauliflower seedlings. Colony morphology and conidial characteristics were identical to the cultures used for inoculation, thereby fulfilling Koch's postulates. Colletotrichum gigasporum has been reported as a causal agent of anthracnose of avocado (Persica americana) in Sri Lanka (Hunupolagama et al., 2015) and of fragrant rosewood (Dalbergia odorifera) in China (Wan et al., 2018). Colletotrichum gigasporum has not been reported previously in India. To our knowledge, this is also the first report of anthracnose on cauliflower caused by C. gigasporum. The authors are thankful to Mahyco Research Centre, Maharashtra for support and suggestions.