Premna integrifolia (Ganimari) is a precise widespread medicinal herb developed mainly in Sri Lanka and different regions of India. The plant grows wild in coastal districts of Andhra Pradesh, Orissa, West Bengal, Tamil Nadu and Kerala in India. Premna integrifolia, a valuable herb in Ayurveda, has many health advantages including analgesic, anti-inflammatory, antibacterial, antiarthritic, antioxidant, anti-ulcer, antidiabetic, CNS stimulant, hepatoprotective, cardiac stimulant, hypolipidemic and neurodegenerative disease-fighting properties. This review discusses the challenges and analytical approaches for determining the chemical components of Premna integrifolia, both quantitatively and qualitatively. This review also provides a thorough analysis of the methods used to accurately characterize the chemical profile of Premna integrifolia extract. The effectiveness of analytical methods like chromatography, UV-spectroscopy and mass spectrometry in identifying and quantifying bioactive constituents is being investigated.