Bananas are the most widely produced fruit in the world. Crown rot, one of the main postharvest diseases of banana fruit, causes significant losses during storage, transportation, and marketing. Different fungi, especially species of the genus Lasiodiplodia, are associated with this disease. Lasiodiplodia isolates were obtained from asymptomatic fruits in the states of Alagoas, Ceará and Pernambuco, Brazil. On Potato Dextrose Agar (PDA), the isolates initially produced moderately dense grayish-white mycelium, then turned olive green to grayish-black, with olive-green or black reverses. The fungal isolate was classified as Lasiodiplodia hormozganensis and Lasiodiplodia laeliocattleyae based on morphological and multigene (TEF1-α, ITS and TUB2) phylogenetic analysis. Pathogenicity tests were performed by inoculating mycelial discs containing pathogen structures on banana fruits, confirming Koch's postulates. This is the first report of L. hormozganensis and L. laeliocattleyae causing crown rot disease in banana plants in the world.