This study aims to identify the determining factors in the digital start-up valuation at the agro seed funding platform. To have a clear understanding, firstly this study provides a conceptual framework, and secondly, evaluates the digital start-up valuation for seed funding platforms (stages) by expert opinions from relevant experience. The study findings are important to develop further digital start-up business for the new agro seed funding. This study identifies that the determinant factors in digital start-up valuation are not merely based on financial aspects, but also based on subjective judgmental factors. The result indicates that the determining factors in digital start-up valuation at the seed funding stage are divided into the micro, meso and macro-levels of analysis. Each level of analysis focus on various aspects, e.g., micro-level on individual and commercial aspects, the meso-level on organisational and corporate aspects, and the macro-level on industry aspects. The findings of this study will be useful in the digital start-up valuation process and will provide guidelines for digital start-up businesses to improve their commercial performance.