Starch Flour Research Articles

Proximate Analysis On Nile Tilapia Artificial Feed From Shrimp Waste

Nile Tilapia (Oreochromis niloticus) is the most widely cultivated freshwater fish because it can grow well in tropical countries and temperate regions. This research aims to create artificial feed that matches the nutritional parameters needed by nile tilapia. This artificial feed will use the basic ingredients of shrimp waste, with addition of oilcake, bran, starch flour, vitamins, squid oil, and water. There are four variables used to make artificial feed with different basic and additional ingredients, as well as varied processing techniques. After artificial feed is completed, proximate analysis will be carried out to test composition of crude fiber, carbohydrates, water, fat, ash, and protein using various methods. The test method for the composition of crude fiber, carbohydrates, fat, ash, and protein follows the method set by the Association of Official Analytical Chemists (AOAC). Based on the results of proximate analysis, variable 1 (V1) has fulfilled the nutritional content of protein and moisture content needed by nile tilapia. This shows that artificial feed formulations from shrimp waste can be used because protein is an important element for nile tilapia growth, but the content of crude fiber, carbohydrates, fat, and ash exceeds ideal value for nile tilapia nutrition. This also occurs in variables 2, 3, and 4 that have not met nutritional needs, especially protein. However, the composition of artificial feed with shrimp waste and addition of meal, bran, starch flour, vitamins, and water in V1 can be further investigated to reduce the content of crude fiber, carbohydrates, fat, and ash.

Physico-Chemical and Organoleptic Characteristics of Cookies Made from Snake Fruit Cultivar Pondoh Flour (Sallaca edulis Reinw.) and Palm Stem Starch (Arenga pinnata Merr.)

Snake fruit is a food that has a fairly high fiber content and also has vitamin C content. Palm stem starch has starch content so it is used to soften the texture of cookies and also has a high fiber content. The use of snake fruit and palm stem starch aims to produce a cookie formulation that is healthier than commercial cookies with high fiber and vitamin C content. The purpose of this study was to determine the physico-chemical and organoleptic properties of cookies made from snake fruit cultivar pondoh flour and palm stem starch. This study used a completely randomized design (CRD) consisting of 5 formulations with 3 replications, namely the ratio of palm stem starch flour and snake fruit cultivar pondoh flour 100%:0% (F1), 75%:25% (F2), 50%:50% (F3), 25%:75% (F4) and 0%:100% (F5). Data were analyzed using One-Way ANOVA and Duncan's further test with 5% significance level. The experiment was conducted by testing physico-chemical and organoleptic properties on 30 untrained panelists. The results showed that making cookies made from snake fruit cultivar pondoh flour and palm stem starch flour had a significant effect on the physico-chemical and organoleptic properties of cookies. The results obtained are water content 4,96%-6,77%; ash content 1,97%-2,88%; fat content 11,78%-14,37%, protein content 6,34%-8,48%; carbohydrate content 57,05%-63,66%; crude fiber content 9,45%-12,25%; vitamin C content 1,00 mg- 2,87 mg; hardness 154,55 N-523,14 N; color L* 29,39-66,05; color a* 2,27-7,84; color b* 10,92-33,86; and organoleptic test color 1,80-4,43; aroma 2,57-3,92, texture 1,87-4,43, taste 1,97-4,23, overall 2,20-4,30.

Microwave‐assisted treatment for the improvement of rice flour properties and rice flour bread quality

SummaryModification of rice flour by microwave and its effect on bread making performance was evaluated. Rice flour having two different water content (20% and 30%) were exposed to different level of microwave power (600 W, 700 W and 800 W) with 8 min treatment time to assess its influence on amylose content, thermal properties, morphological structure, functional and crystalline structural that affect bread quality. Microwave‐treated rice flour at 600 W showed notable increment in amylose content of 1.03 folds (for both 20% and 30% water content) as compared to untreated rice flour. The microwave treatment destroyed the amorphous region of the rice flour starch, whereas starch crystal structure was slightly increased after treatment. Rice flour maintained its thermal stability even after exposure to high radiation of microwave. The treated rice flour exhibited starch granules with more voids on its surface, indicating the exudation of amylose at higher temperature. The major effect of microwave treatment was observed on the bread for rice flour treated at 800 W with higher specific volume, increase in gas cells pores, softer crumb, low hardness and delayed staling as compared to untreated rice flour. The microwave‐assisted treatment shows a significant potential in improving the rice flour quality led to increase the quality of bread.

Effects of soaking treatment on water distribution of rice grains, starch characteristics and eating quality of wet rice noodles

Herein, rice was subjected to different soaking processes on water distribution of rice grains, starch characteristics, and eating quality of fresh wet rice noodles. Results demonstrated that, when soaked at temperatures between 10 °C and 40 °C for 120 min, rice grains reached saturation in water absorption, and the hardness gradually stabilized. However, the moisture continued to penetrate the interior of rice grains after 120 min, leading to an increase in moisture content, higher water permeability, and enlarged water migration channels. With extended soaking time periods, the content of damaged starch in rice flour considerably decreased. Although the gelatinization temperature of rice starch decreased after soaking, the enthalpy required for gelatinization increased. The relative crystallinity of rice starch demonstrated an increasing trend, followed by a decreasing trend, and reached its highest value of 18.18 % after 60 min of soaking. To summarize, the texture indices of fresh rice noodles demonstrated an increasing trend, although stretching and cooking quality demonstrated a trend of initially increasing and then decreasing with no considerable changes observed between 120 and 240 min of soaking. In summary, moderate soaking treatment can enhance the edible quality of fresh wet rice noodles.

The multifactorial phenomenon of enzymatic hydrolysis resistance in unripe banana flour and its starch: A concise review.

Unripe banana flour starch possesses a high degree of resistance to enzymatic hydrolysis, a unique and desirable property that could be exploited in the development of functional food products to regulate blood sugar levels and promote digestive health. However, due to a multifactorial phenomenon in the banana flour matrix-from the molecular to the micro level-there is no consensus regarding the complex mechanisms behind the slow enzymatic hydrolysis of unripe banana flour starch. This work therefore explores factors that influence the enzymatic hydrolysis resistance of raw and modified banana flour and its starch including the proportion and distribution of the amorphous and crystalline phases of the starch granules; granule morphology; amylose-amylopectin ratio; as well as the presence of nonstarch components such as proteins, lipids, and phenolic compounds. Our findings revealed that the relative contributions of these factors to banana starch hydrolytic resistance are apparently dependent on the native or processed state of the starch as well as the cultivar type. The interrelatability of these factors in ensuring amylolytic resistance of unripe banana flour starch was further highlighted as another reason for the multifactorial phenomenon. Knowledge of these factors and their contributions to enzymatic hydrolysis resistance individually and interconnectedly will provide insights into enhanced ways of extraction, processing, and utilization of unripe banana flour and its starch.

Energy-size relationship and starch modification in planetary ball milling of quinoa

The effects of speed (250–450 rpm) and time (10–50 min) on milling energy (E), particle size distribution (PSD), crystallinity degree (CD), damaged starch (DS), microstructure, hydration and pasting properties of flours obtained in a planetary ball mill (PBM) were investigated. As increasing milling severity quinoa flours showed polydispersed PSD (peaks at 417, 40 and 2 μm) with a shift towards smaller size and greater dispersion (Span: 3.0–5.2) as well as particles with rounded edges and polished surfaces (SEM images). The relationship E − D50 were satisfactorily predicted by the Walker's equation. The hydration tests as function of temperature and milling conditions revealed a complex flour behavior due to differences in PSD, composition, and DS content. The increasing milling energy caused a decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of up to 36%, 29%, and 25%, respectively. Significant correlations between flour attributes were found (DS-CD, r = −0.83, p < 0.01; PV-D50, r = 0.92, p < 0.01; TV-D50, r = 0.94, p < 0.01; FV-D50, r = 0.90, p < 0.01) denoted the increase of starch degradation as milling severity rises. These results can be used to improve the manufacture and the selection criteria of quinoa flour with specific functional attributes.

Effect of chickpea thermal treatments on the starch digestibility of the fortified biscuits

Flour of roasted and heat-moisture treated chickpeas was used as a partial replacement of wheat flour, facilitating the development of biscuits with delayed digestion properties. The multiscale structural properties of starch and microstructural changes in chickpea flour and fortified biscuits were analyzed. Chickpea thermal treatment reduced the starch digestibility of fortified biscuits by 5.43%–7.33%, enhanced the resistant starch content of chickpea flour by 7.86%–12.28%. For thermal-treated chickpea fortified biscuits, the degree of relative crystallinity, short-range order, and double helix of starch was enhanced, the pasting viscosity was reduced, and the heat-moisture treatment showed a significant effect. Scanning electron microscope showed that fortified biscuits had a continuous and denser microstructure, reducing the accessibility of enzymes to hydrolyze starch molecules. The glycemic index values of fortified biscuits were reduced to below 66 in mice, as seen by their postprandial blood glucose response. Altogether, these results provide guidance for the development of delayed digestion meal replacement biscuits.

Effects of adding blueberry residue powder and extrusion processing on microstructure and in vitro digestibility of indica rice flour

Using crushed indica rice flour as the main raw material, after adding blueberry residue powder, the indica rice puffed powder containing blueberry residue (IREPBR) was obtained through extrusion and crushing. The effects of adding blueberry residue and extrusion processing on the microstructure and in vitro digestibility of indica rice flour were studied. The basic component analysis results showed that the fat and total starch content of indica rice powder significantly decreased after adding blueberry residue powder and extrusion puffing, while the total dietary fiber content significantly increased. The results of scanning electron microscope (SEM) showed that there were many pits and bubbles in the extruded indica rice flour after adding blueberry residue powder. Compared with indica rice flour (IRF), its Water Absorption Index (WAI) and Water Solubility Index (WSI) significantly increased, and its thermal properties also changed. XRD and FT-IR analysis showed that after extrusion, the crystalline morphology of indica rice starch changed from A-type to V-type, and the relative crystallinity also changed. In addition, the content of resistant starch (RS) in indica rice flour added with blueberry residue powder and processed by extrusion increased significantly, while the starch hydrolysis rate and estimated blood glucose index (eGI) decreased.

Physicochemical, Cooking Quality and Sensory Acceptability of Three Cowpea Varieties Grown in Botswana

Background: Information on the physicochemical, cooking and sensory qualities of cowpea seeds are required for nutrition information, to design processing and for consumer acceptance. In view of this, three cowpea varieties (Tswana, Blackeye and ER7) grown in Botswana were evaluated. Method: Proximate compositions, physical, gelatinization and sensory properties respectively were evaluated following AOAC, conventional, Amylograph and on 7-point hedonic sensory acceptability scale. Result: Significant variations (p less than 0.05) were observed in fat, fiber, ash and available carbohydrate (CHO) contents, length, thickness, hundred seeds weight (HSW), hydration capacity, hydration index, cooking time, flour starches maximum gelatinization temperature (Tm,°C) and viscosity at Tm. The percentage moisture, protein, fiber, fat, ash and available carbohydrate contents ranged 9.5-10.1, 21.7-22.8, 2.3-2.6, 1.1-2.0, 2.1-3.0 and 59.4-62.3, respectively. Initial gelatinization temperature (To,°C) and Tm (°C) were ranged 71.3(ER7)-73.7(Tswana) and 83.0(ER7)-84.7(Blackeye), respectively. Significant (p less than 0.05) variations in Tm and viscosity at Tm were observed because of differences in water uptake, swelling and thickening ability at high temperature. Longest cooking time was recorded for Tswana (43.57 min) while shortest was for Blackeye (36.10 min). Tswana recorded a longer cooking time probably because of low water hydration capacity, small seed size and low HSW. There was no significant difference in the sensory liking of the cooked cowpea seeds and were rated from slightly to moderately like. Cowpeas of high protein and fiber contents, short cooking time and high thickening ability on cooking are preferred for healthy diets. In terms of cooking time and thickening ability, Tswana variety appeared poor. Shorter cooking time, high protein content and high thickening ability of Blackeye cowpea is more acceptable. The ER7 is preferred for its large seed size.

Higher Grain-Filling Rate in Inferior Spikelets of Tolerant Rice Genotype Offset Grain Yield Loss under Post-Anthesis High Night Temperatures

Increased nighttime respiratory losses decrease the amount of photoassimilates available for plant growth and yield. We hypothesized that the increased respiratory carbon loss under high night temperatures (HNT) could be compensated for by increased photosynthesis during the day following HNT exposure. Two rice genotypes, Vandana (HNT-sensitive) and Nagina 22 (HNT-tolerant), were exposed to HNT (4 °C above the control) from flowering to physiological maturity. They were assessed for alterations in the carbon balance of the source (flag leaf) and its subsequent impact on grain filling dynamics and the quality of spatially differentiated sinks (superior and inferior spikelets). Both genotypes exhibited significantly higher night respiration rates. However, only Nagina 22 compensated for the high respiration rates with an increased photosynthetic rate, resulting in a steady production of total dry matter under HNT. Nagina 22 also recorded a higher grain-filling rate, particularly at 5 and 10 d after flowering, with 1.5- and 4.0-fold increases in the translocation of 14C sugars to the superior and inferior spikelets, respectively. The ratio of photosynthetic rate to respiratory rate on a leaf area basis was negatively correlated with spikelet sterility, resulting in a higher filled spikelet number and grain weight per plant, particularly for inferior grains in Nagina 22. Grain quality parameters such as head rice recovery, high-density grains, and gelatinization temperature were maintained in Nagina 22. An increase in the rheological properties of rice flour starch in Nagina 22 under HNT indicated the stability of starch and its ability to reorganize during the cooling process of product formation. Thus, our study showed that sink adjustments between superior and inferior spikelets favored the growth of inferior spikelets, which helped to offset the reduction in grain weight under HNT in the tolerant genotype Nagina 22.

Study of molecular dynamic interactions during the optimized extrusion processing of corn (Zea mays) and substandard bean (Phaseolus vulgaris)

Within the agri-food industry, substantial quantities of by-products are generated during the collection of raw materials and the preparation of derived food products. A common by-product is the low-value substandard bean that might have structural damages but retains its nutritional characteristics. This research aimed to evaluate the compatibility of bean protein with corn flour starch within an extruded snack model through molecular dynamics simulation by the calculation of the solubility parameter (δ). For the extrusion processing, a central composite design was used with four factors: temperature [150–190 °C], screw speed [150–210 rpm], moisture content [14–18%] and substandard bean content [20–80%]. Simulation calculations were performed using Materials Studio 8.0 software (Accelrys, San Diego, CA). The bean protein was identified as ARC5A, and its structure was obtained from the UniProt database. Starch and protein models evaluated within a temperature profile [25–150 °C] emulating the extrusion processing showed Δδ < 7.0 MPa0.5, indicating that are likely miscible. Optimal processing conditions of the extruded snack were obtained. This research developed an extruded snack using an agro-industrial by-product while predicting the solubility and miscibility parameters of more compounds using molecular dynamics simulations.

Comparison of noodle-making performance of purple-colored whole wheat flour prepared with a jet mill and an ultra-centrifugal mill.

This study explores the impact of milling methods on the quality and noodle-making performance by comparing jet-milled (WF-JM) and ultra-centrifugally milled (WF-UM) purple-colored whole wheat flours. WF-JM exhibits smaller starch granules and a fragmented protein matrix attributed to the increased milling pressure. Physicochemical analyses reveal lower moisture and higher damaged starch in WF-JM. Rheological analyses show lower viscosity in the WF-JM blends. The mixograph results reveal weaker dough-mixing stability and strength for WF-JM. Cooked noodles from WF-JM are uneven, in contrast to uniform WF-UM strands. Blending WF-UM enhances noodle quality. Overall, the noodle-making performance for WF-JM was inferior compared to WF-UM, confirming the significantly negative impact of damaged starch and fragmented protein matrix in whole wheat flour than the positive effect of particle size. This study highlights the complex interplay between milling methods, particle size, and physicochemical attributes, providing insights for optimizing whole wheat flour processing and product quality.

Interactions between partially gelatinized starch and nonstarch components in potato flour and their performance in emulsification

To develop natural complex materials as starch-dominated emulsifiers, pregelatinization was conducted on potato flour. The effects of gelatinization degrees (GDs, 0 %–50 %) on the structural characteristics, physicochemical properties, and emulsifying potentials of potato flour were investigated. Increasing GD of potato flour promoted protein aggregation on starch granules surfaces and transformed starch semicrystalline structures into melted networks. The emulsion stabilized with 50 % GD potato flour exhibited excellent storage stability (7 d) and gel-like behavior. With increasing GD from 0 to 50 %, the respective apparent viscosities and elastic moduli of emulsion increased from 21.4 Pa to 1126.7 Pa, and from 0.133 Pa·s to 1176.6 Pa·s, promoting the formation of a stable network structure in the emulsion. Fourier transform infrared spectra from emulsions with a continuous phase of >20 % GD displayed a new peak around 1740 cm−1, suggesting improved covalent interactions between droplets, thereby facilitating emulsion stability. Confocal laser scanning microscopy images indicated that droplets could be anchored in the melted networks and broken starch granules, inhibiting droplets coalescence. These results suggest that pregelatinization is a viable strategy for customizing natural starch-dominated emulsions.

Synthesis and Characterization of Briquette from Carbonized Pinus Patula Saw Dust as Alternative Energy Source

This study aimed at the synthesis and characterization of briquette from Pinus patula saw dust using different binders. The effects of particle size and type of binder on the fuel qualities of the briquette were investigated. The bulk density of biomass causes handling, storage, and transportation problems that limit its large application. Densification of biomass into briquettes can solve these problems. The experimental results indicate that briquette produced from waste paper, fruit waste, cow dung, and starch flour binders has higher calorific value, higher fixed carbon, and lower ash content. Whereas, molasses and wood ash binders decreased the calorific value and fixed carbon and increased the ash content of the briquette. As a result, a maximum calorific value of 6596 cal/g, fixed carbon content of 62.6%, and ash content of 3.66% are obtained using waste paper. The calorific value of 6232 cal/g, fixed carbon of 48.74%, and ash content of 3.33% was obtained using fruit waste while the calorific value of 6194 cal/g, fixed carbon content of 59.94 %, and ash content of 5.35% was observed when cow dung is used. When starch flour is used, a calorific value of 6170 cal/g, fixed carbon content of 54.63%, and ash content of 2.63 was obtained. Therefore, waste paper, fruit waste, cow dung, and starch flour are promising binders that improve the fuel qualities of biomass briquettes.

Unlocking the potential of sorghum: a comparative study exploring the physical, chemical, technological, and digestibility properties of new cultivars from Mozambique

SummaryThis study aimed to evaluate the physical, chemical, technological, and digestibility of three sorghum cultivars (Mucuvea, Otela, and Tocole) and compared them to a Local variety (criola) grown in Mozambique. Thousand‐grain weight, hectolitre weight, and colour were determined in the grains. Proximal composition, antinutritional factors, starch and protein digestibility, and antioxidant activity were analysed in flours; starch was isolated and characterised by its physical, chemical, morphological, and digestibility properties. The grain characterisation varied among the cultivars compared to the Local variety, but not between the cultivars. The cultivars showed lower amounts of protein, lipids, free phenolics, tannin, soluble protein, digestibility of starch flour and proteins, and higher flavonoids and antioxidant activity. A high content of amylose (~37%), glycaemic index (~82), Tp (64.83 °C), and resistant starch (57.73%) were verified in all cultivars. The peak viscosity of Local variety was higher (343.25 RVU) than the other cultivars, while the breakdown (less than 106.54 RVU) of the cultivars was significantly lower than Local (182.25 RVU). Such data suggest great potential use of Local variety in bakery products and the cultivars in products that require greater viscosity. These results will reinforce the research, production, and manufacture of new products in Africa, especially in sorghum‐producing countries.

Computational modelling of extrusion process temperatures on the interactions between black soldier fly larvae protein and corn flour starch

Insects such as the black soldier fly (BSF) are recently being studied as food sources to address concerns about how to meet the food demand of the growing world population, as conventional production lines for meat proteins are currently unsustainable sources. Studies have been conducted evaluating the use of insect proteins to produce extruded foods such as expanded snacks and meat analogues. However, this field of study is still quite new and not much has been studied beyond digestibility and growth performance. The purpose of this work was to evaluate the compatibility of protein extracted from BSF flour with corn flour starch within an extruded balanced shrimp feed model through molecular dynamics simulations, for which cohesive energy density and solubility parameter (δ) of both components were determined. The calculations’ results for the protein molecule systems yielded an average δ of 14.961 MPa0.5, while the δ for starch was calculated to be 23.166 MPa0.5. The range of difference between both δ (10 > δ > 7) suggests that the interaction of the BSF protein with corn starch is of a semi-miscible nature. These results suggest that it is possible to obtain a stable starch-protein mixture through the extrusion process.

Preparation of Multi‐Grain Flour with High Content of Resistant Starch and the Mechanism Underlying the Improved Digestion Resistance

AbstractTo acquire the optimum treatment strategy that leads to the highest yield of resistant starch (RS) in multigrain flour (MF), wheat flour, whole oat flour, and whole buckwheat flour are used to prepare antidigestive MF by using pullulanase debranching combining heat–moisture treatment (P‐HMT). Based on the single factor tests and response surface analysis, the optimum conditions for the treatment of MF are determined as follows: pullulanase 62.6 U g−1, autoclaving 21.3 min, HMT at 100 °C, and 30.4% water content for 6.0 h. Under these conditions, the yield of RS in the flour reaches 77.42%. P‐HMT causes protein denaturation, starch gelatinization, and flour clumps in MF, facilitating starch recrystallization and interactions among short‐chain starch, monomeric proteins, and lipids. All these findings are further confirmed by the conversion of starch crystal from A‐type to B+V‐type, the improvement of starch short‐range molecular order, and the presence of more binary/ternary complexes in treated flour. In brief, the starch digestion resistance improvement in the treated flour is attributed to the recrystallization of short‐chain starch and interactions between starch and protein/lipid.

Large flour aggregates containing ordered B + V starch crystals significantly improved the digestion resistance of starch in pretreated multigrain flour

The starch digestibility of flour is influenced by both physicochemical treatment and flour particle size, but the interactive effect of these two factors is still unclear. In this study, the effect of pullulanase debranching, combined with heat-moisture treatment (P-HMT), on starch digestibility of multi-grain flours (including oat, buckwheat and wheat) differing in particle size was investigated. The results showed that the larger-size flour always resulted in a higher resistant starch (RS) content either in natural or treated multi-grain flour (NMF or PHF). P-HMT doubled the RS content in NMFs and the large-size PHF yielded the highest RS content (78.43 %). In NMFs, the cell wall integrity and flour particle size were positively related to starch anti-digestibility. P-HMT caused the destruction of cell walls and starch granules, as well as the formation of rigid flour aggregates with B + V starch crystallite. The largest flour aggregates with the most ordered B + V starch were found in large-size PHF, which contributed to its highest RS yield, while the medium- and small-size PHFs with smaller aggregates were sensitive to P-HMT, resulting in the lower ordered starch but stronger interactions between starch and free lipid or monomeric proteins, eventually leading to their lower RS but higher SDS yield.

Effect of annealing on the chemical and physico- functional properties of fermented provitamin A cassava starch

This study reports the effect of annealing on chemical and physico-functional properties of fermented pro-vitamin A cassava starch of cassava roots TME-IBA 070539 variety. The provitamin cassava starch flour was extracted to produce native starch flours (NCS). Fermentation was carried out on the tubers at 24 and 48 hours to produce provitamin A cassava fermented starches FCS24 and FCS48 respectively. The starch flour samples NCS, FCS24 and FCS48 were then annealed at 50°C to produce annealed native starches (ANCS), annealed 24 hours fermented cassava starch (AFCS24) and annealed 48 hours fermented cassava starch (AFCS48). The chemical and physico-functional properties were evaluated using standard methods. The result revealed negligible amount of crude protein and ash content. However, the carbohydrate content in all the samples ranged between 93.39 – 95.01% signifying high purity starches. These processes imparted positively the β- carotene content which ranged between 2.50 – 2.80 µg/100g in the cassava starches. Although, no significant (p&gt;0.05) difference was observed in the β-carotene content when compared with the corresponding annealed starches except for a slight reduction at AFCS48. The amylose content decreased significantly (p&gt;0.05) by 21.72 and 38% in the combined process of annealing and fermentation as observed for AFCS24 and AFCS48 respectively. Also, amylose leaching was more pronounced in the NCS than the fermented and annealed starches. No significantly (p&lt;0.05) difference was observed in the corresponding samples; ANCS, AFCS24 and AFCS48 for total titratable acidity while pH decreased significantly in the annealed starches. The swelling and solubility increased with increase in temperature, generally annealing lowered the swelling capacity and increased the solubility of the starch samples and this could be harnessed in food preparations and in the industries.

Optimization of Tempe Fermentation With the Addition of a Combination of Yeast and Starch Flour: Effect on Process Speed and Product Freshness Period

This research explores the potential for adding a combination of tempeh yeast and starch to the tempeh fermentation process to speed up fermentation and extend the product's freshness period. Through an experimental approach, we investigated the effect of the proportion of yeast and starch on the fermentation speed and shelf life of tempeh. We found that a yeast to starch ratio of 1:2 significantly accelerated the fermentation process, while a ratio of 1:1 was effective in extending the shelf life of tempeh up to 80 hours. Organoleptic tests show positive consumer acceptance of the tempeh variations produced. This research makes a significant contribution to tempeh processing technology, offering practical solutions for the fermented food industry to improve the quality and attractiveness of tempeh products on the market.&#x0D;